A survey to investigate shortfalls in the dental care professional (DCP) workforce in South Yorkshire in 2004.

AIM: To determine the composition of the dental care professional (DCP) workforce in South Yorkshire, the existence of any training requirements, and future intentions and motivations relating to the provision of National Health Service (NHS) dental care. METHODS: The study used mixed methods and incorporated a cross-sectional postal questionnaire and focus groups. The questionnaire was sent to all NHS dental practices in South Yorkshire (n=201) for completion by a principal general dental practitioner (GDP). Focus groups were held with GDPs in these practices. RESULTS: Responses were received from principal GDPs at 156 practices (78%). A total of 624 dental nurses, 65 dental hygienists and 24 dental therapists were employed in the responding General Dental Services (GDS) practices, representing whole-time equivalents (WTEs) of 458.5, 18.5 and 10.1, respectively. Many practices had current vacancies for DCPs, with 24.9 WTE available for dental nurses, 3.7 WTE for dental hygienists, and 5.0 WTE for dental therapists. Workforce shortages were evident among dental nurses (5.2%), dental hygienists (16.7%), and dental therapists (33.0%). Principal GDPs suggested that improved terms and conditions of employment for DCPs, particularly dental nurses, might aid recruitment and retention in NHS practice. CONCLUSIONS: Respondents perceived that there were shortages in the DCP workforce in South Yorkshire. Initiatives are required to address these shortages.

A novel method for exploring elemental composition of microbial communities: laser ablation-inductively coupled plasma-mass spectrometry of intact bacterial colonies.

Bacterial colonies are spatially complex structures whose physiology is profoundly dependent on interactions between cells and with the underlying semi-solid substratum. Here, we use bacterial colonies as a model of a microbial community to evaluate the potential of laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) to delineate elemental distributions within colonies with minimal pre-treatment. To reduce water content of the colony and limit undesirable absorption of laser energy, we compared methods of preparing 24h-old colonies of Escherichia coli TG1 on agar for laser ablation. Colonies on excised agar segments dried on chromatography paper were superior to colonies dried in a dessicator or by prolonged incubation, with respect to signal magnitude, signal:noise ratio and background signal. Having optimised laser scan speed (10 microm s(-1)) and laser beam diameter (100 microm), further improvements were achieved by growing colonies on nylon membranes over agar, which were then transferred to the ablation chamber without further treatment. Repeated line rasters across individual membrane-supported colonies yielded three-dimensional elemental maps of colonies, revealing a convex morphology consistent with visual inspection. By normalising isotope counts for P, Mn, Zn, Fe and Ca against Mg, the most abundant cellular divalent cation, we sought elemental heterogeneity within the colony. The normalised concentration of Mn in the perimeter was higher than in the colony interior, whereas the converse was true for Ca. LA-ICP-MS is a novel and powerful method for probing elemental composition and organisation within microbial communities and should find numerous applications in, for example, biofilm studies.