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1.
Mikrochim Acta ; 188(11): 399, 2021 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-34716816

RESUMO

Pathogenic bacteria can cause the outbreaks of disease and threaten human health, which stimulates the development of advanced detection techniques. Herein, a specific and sensitive electrochemical biosensor for Gram-negative bacteria was established based on the conductive polymer with artificial muscle properties.  The effective recognition was achieved through the specific carbohydrate-carbohydrate interaction between gluconamide and lipopolysaccharide.  The application of impulse voltage enhances the efficiency of recognition and shortens the detection time through the temporary deformation of the electrode surface, with a limit of detection (LOD)  of 1 × 100 CFU/mL and a linear range of 1 × 100 - 1 × 106 CFU/mL for Escherichia coli (E. coli). In addition  to the merits of low cost, high efficiency, and rapidity,  the developed label-free electrochemical biosensor can also be applicable for other Gram-negative bacteria, owning promising potential in the application of portable devices and paving a potential way for the construction of electrochemical biosensors.


Assuntos
Técnicas Biossensoriais/métodos , Escherichia coli/isolamento & purificação , Gluconatos/química , Lipopolissacarídeos/química , Pseudomonas putida/isolamento & purificação , Animais , Técnicas Biossensoriais/instrumentação , Água Potável/microbiologia , Técnicas Eletroquímicas/instrumentação , Técnicas Eletroquímicas/métodos , Eletrodos , Escherichia coli/química , Contaminação de Alimentos/análise , Sucos de Frutas e Vegetais/microbiologia , Limite de Detecção , Leite/microbiologia , Nanoestruturas/química , Polímeros/química , Pseudomonas putida/química , Pirróis/química , Rios/microbiologia , Poluentes da Água/análise
2.
J Mater Chem B ; 9(8): 2016-2024, 2021 03 04.
Artigo em Inglês | MEDLINE | ID: mdl-33544115

RESUMO

Nanomaterial-based artificial enzyme mimetics have attracted increasing attention because of their robust stability, adjustable activity, and cost-effectiveness. In this study, we developed a simple and effective method for the synthesis of highly dispersed ultrafine PdCo alloys with peroxidase- and catalase-like activities. The aberration-corrected transmission electron microscopy analysis verified that the cyanogel precursor in the mesoporous silica nanospheres (MSNs) was converted to PdCo alloy in NH3 at a high temperature. The PdCo alloy was homogenously distributed in MSNs as ultrafine and monodispersed particles. By selectively removing the Co species from the binary alloy through an acid-leaching approach, the role of each component in the enzyme-like mimetics was systematically studied. Using glutathione (GSH) as the model analyte, the potential application of PdCo@MSNs in GSH detection from complex cell media was confirmed via colorimetric assay. The ultrafine alloy size, double mimetic activities, and abundant loading space of PdCo@MSNs make them promising not only in clinical diagnosis but also in overcoming hypoxia-induced photodynamic therapy resistance in tumor treatment.


Assuntos
Ligas/química , Materiais Biomiméticos/química , Catalase/metabolismo , Desenho de Fármacos , Nanopartículas/química , Peroxidase/metabolismo , Dióxido de Silício/química , Cobalto/química , Glutationa/análise , Paládio/química , Porosidade , Temperatura
3.
ACS Appl Mater Interfaces ; 11(19): 17215-17225, 2019 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-31007010

RESUMO

As a highly oxygen-dependent process, the effect of photodynamic therapy is often obstructed by the premature leakage of photosensitizers and the lack of oxygen in hypoxic cancer cells. To overcome these limitations, this study designs bovine serum albumin protein (BSA)-encapsulated Pt nanoclusters (PtBSA) as O2-supplied biocoats and further incorporates them with mesoporous silica nanospheres to develop intelligent nanoaggregates for achieving improved therapeutic outcomes against hypoxic tumors. The large number of amino groups on BSA can provide sufficient functional groups to anchor tumor targeting agents and thus enhance the selective cellular uptake efficiency. Owing to the outstanding biocompatibility features of BSA and the state-of-the-art catalytic activity of Pt nanoclusters, the nanocomposites have lower dark cytotoxicity, and O2 continuously evolves via the decomposition of H2O2 in a tumor microenvironment. Both in vivo and in vitro experiments indicate that the resulting nanocomposites can effectively relieve hypoxic conditions, specifically induce necrotic cell apoptosis, and remarkably hinder tumor growth. Our results illuminate the great potential of BSA-encapsulated Pt nanoclusters as versatile biocoats in designing intelligent nanocarriers for hypoxic-resistant photodynamic therapy.


Assuntos
Materiais Biocompatíveis/farmacologia , Nanoestruturas/administração & dosagem , Neoplasias/terapia , Fotoquimioterapia , Células A549 , Animais , Apoptose/efeitos dos fármacos , Materiais Biocompatíveis/química , Proliferação de Células/efeitos dos fármacos , Xenoenxertos , Humanos , Peróxido de Hidrogênio/química , Peróxido de Hidrogênio/farmacologia , Camundongos , Nanocompostos/química , Nanoestruturas/química , Neoplasias/patologia , Oxigênio/metabolismo , Fármacos Fotossensibilizantes/química , Fármacos Fotossensibilizantes/farmacologia , Soroalbumina Bovina/química , Soroalbumina Bovina/farmacologia , Dióxido de Silício/química , Dióxido de Silício/farmacologia , Hipóxia Tumoral/efeitos dos fármacos , Microambiente Tumoral/efeitos dos fármacos
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