Determination of Environmental Micro(Nano)Plastics by Matrix-Assisted Laser Desorption/Ionization-Time-of-Flight Mass Spectrometry.

Micro(nano)plastics (MNPs) are widely acknowledged as global environmental threat while determination methods for MNPs are still lacking and becoming a growing concern. This study provides a novel method for MNPs identification/quantification by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS). Factors affecting the measurement were optimized, including laser energy, matrix (M), analyte (A), cationization agent (C), and MAC volume ratio. Under the optimal conditions, the peaks representative of polystyrene (PS) and polyethylene terephthalate (PET) were identified, and the mass differences were consistent with the molecular weight of the corresponding oligomer. A quantitative correlation was built between normalized signal intensity and ln[polymer concentration] with a correlation coefficient above 0.96 for low-molecular-weight polymers and 0.98 for high-molecular-weight polymers. Furthermore, two types of environmental MNP samples were prepared, including aviation cup particles as fresh plastics and aged MNPs extracted from river sediment. By using MALDI-TOF MS, the PS-related MNPs (in both aviation cup and sediment) consisted of C8H8 and C16H16O oligomers, while the PET-related MNPs (only found in sediment) were identified with repeated units of C10H8O4 and C12H12O4. According to the quantitative correlation curve, the contents of PS and PET MNPs were quantified as 8.56 ± 0.04 and 28.71 ± 0.20 mg·kg-1, respectively, in the collected sediment. This study is the first attempt to propose a quantification method with the employment of MALDI-TOF MS for aged MNPs analysis in environmental samples, which can not only supply an effective method for MNP analysis but also inspire future studies on the in situ distribution and transformation of MNPs in environmental and biological samples.

Microplastic-induced gut microbiota and serum metabolic disruption in Sprague-Dawley rats.

Toxic effects of exposure to microplastics (MPs) on living organisms and humans have attracted global concern. However, most previous studies exposed animals to only one type of MP (mainly polystyrene) to assess the health risk of MPs for animals. Therefore, we conducted a laboratory gavage experiment on rats based on the types and concentration of MPs to which humans are exposed in their daily life. The purpose of this study is to use Sprague-Dawley (SD) rat models to assess the potential health risks in mammals from co-exposure to various MPs. In the present study, SD rats were exposed to 12 mg/kg bw/day mixed-MPs (containing 10 types of MPs) for 42 days, and then examined the alteration of gut microbes and serum metabolites. The results showed that 6 gut microbes at the family level (f_Muribaculaceae, f_Oscillospiraceae, f_Bacteroidaceae, f_Neisseriaceae, f_Prevotellaceae, and f_Veillonellaceae) were significantly perturbed (t-test, p < 0.05) in rats after MP exposure. After MP intervention, 47 metabolites significantly regulated in SD rat serum, mainly including lipids and lipid-like molecules (e.g., fatty acids), organic acids and derivatives (e.g., phosphoric acids), and isoflavonoids (e.g., daidzein). These findings contribute to assessing the health risks of various MP co-exposure in mammals in the actual environment and provide a novel insight into the toxicity mechanism of MPs.

Twenty bisphenol analogues in take-out polystyrene-made food containers: concentration levels, simulated migration, and risk evaluation.

Bisphenol A (BPA) is mainly used to produce polycarbonate consumer products. However, the occurrence of BPA and other bisphenol analogues (BPs) in polystyrene-made plastic products, such as white foam take-out containers (WFTOCs), has not been well investigated. In this study, occurrence of 20 BPs in WFTOC samples collected from China, Canada, and Poland were investigated with a sample size of 152. Results showed that 11 out of 20 BPs have been detected at least in one WFTOC sample. Among them, BPA was the most frequently detected BP, followed by bisphenol S (BPS) and bisphenol AF, while BPF was the least detected BP. Very high concentrations of BPA and BPS were detected in WFTOCs from China (mean 2694 and 552 ng/g), compared with Canada (81 and 45 ng/g, respectively) and Poland (95 and 16 ng/g). Other BPs, such as bisphenol TMC (BPTMC; detection frequency 65%, range < LOQ - 224 ng/g), bisphenol G (17%, < LOQ - 11 ng/g), and bisphenol BP (11%, < LOQ - 1.6 ng/g), were also detected in Chinese WFTOC samples. The mean partitioning coefficients of BPA, BPS, bisphenol AP, and BPTMC between WFTOCs and tap water, 10% ethanol, 50% ethanol, corn oil, or steamed rice were 0.22 - 2.9%, 0.16 - 5.1%, 0.11 - 7.5%, 2.3 - 6.5%, or 0.19 - 0.36%, respectively. The estimated daily intake of BPA, BPS, and BPTMC through using WFTOCs were 0.50 - 547, 0.054 - 229, and < 0.66 ng/kg bw/day, respectively, for general population in China, Canada, and Poland. Overall, this study first reveals the unexpected presence of BPs in WFTOCs made of polystyrene, which contributes to the better understanding of the sources of human exposure to BPs.

Microplastics in Antarctic krill (Euphausia superba) from Antarctic region.

Pollution of microplastics (MPs) has become a potential threat to Antarctic marine ecosystems. However, the occurrence of MPs in Antarctic krill (Euphausia superba), a keystone species in Antarctic ecosystems, remains unclear. In this study, the abundance and characteristics of MPs were examined in Antarctic krill samples (n = 437) collected from two Antarctic regions. MPs were extracted using an alkali digestion method and analyzed using Fourier-transform infrared spectroscopy. The mean abundance of MPs in Antarctic krill samples from the South Shetland Islands (n = 355) and the South Orkney Islands (n = 82) were 0.29 ± 0.14 and 0.20 ± 0.083 items/individual, respectively. >90 % of MPs found in Antarctic krill were < 150 µm in size. Fibers represented 77 % and 87 % of the MPs in Antarctic krill samples from the South Shetland Islands and the South Orkney Islands, respectively. Black, blue, and red were the predominant colors of MPs in Antarctic krill, accounting for 32 %, 22 %, and 21 % of the total MPs, respectively. Seven polymer compositions were identified for the MPs in Antarctic krill, with the predominance of polyethylene (37 % of total MPs), followed by polypropylene (22 %) and polyester (21 %). To our knowledge, this is the first study to investigate the occurrence of MPs in Antarctic krill samples. The results of this study are important for evaluating the risks of MP exposure in Antarctic krill.

Microplastics in polystyrene-made food containers from China: abundance, shape, size, and human intake.

Polystyrene-made food containers (PMFCs) have been widely used as takeout containers in China. However, the pollution of microplastics (MPs) in PMFCs used in Chinese restaurants remains not well known. For the first time, this study analyzed MPs in PMFC samples (n = 354) collected from different restaurants in 28 Chinese cities. MPs were detected in all PMFC samples, with an abundance of 5-173 items/container. PMFC samples from Taiyuan (mean of 86 items/container) contained the highest mean abundance of MPs. A relatively lower abundance of MPs was found in PMFCs from Urumqi (mean of 19 items/container) and Fuzhou (18 items/container). Fiber was the predominant shape of MPs in most of the PMFC samples. The abundance of MPs in PMFCs was positively correlated with the proportion of fiber. The major polymer composition of MPs was polystyrene, accounting for a mean of 45-90% of total polymers for MPs in PMFCs from different cities. The abundance of MPs in PMFC samples was negatively correlated with the proportion of polystyrene. The mean estimated oral exposure of MPs for the general population in different Chinese cities was 0.24-1.4 items/kg bw/day. Such data is important for human MP exposure risk assessment and also for elucidating the sources of human exposure to MPs.

Microplastics in terrestrial insects, long-horned beetles (Coleoptera: Cerambycidae), from China.

Despite studies have proposed that microplastics (MPs) could exert adverse effects on terrestrial ecosystems and biota, the occurrence of MPs in wild terrestrial insects has been rarely investigated. This study examined MPs in 261 long-horned beetle (Coleoptera: Cerambycidae) samples collected from four different Chinese cities. Detection frequency of MPs in long-horned beetles from different cities was 68-88 %. Long-horned beetles from Hangzhou (4.0 items/individual) had the highest mean abundance of MPs, followed by that from Wuhan (2.9 items/individual), Kunming (2.5 items/individual), and Chengdu (2.3 items/individual). The mean size of MPs in long-horned beetles from four Chinese cities was 381-690 µm. Fiber consistently represented the major shape of MPs in long-horned beetles from different Chinese cities, contributing 60, 54, 50, and 49 % of total items of MPs in Kunming, Chengdu, Hangzhou, and Wuhan, respectively. Polypropylene was the major polymer composition of MPs in long-horned beetles from Chengdu (68 % of total items of MPs) and Kunming (40 %). However, polyethylene and polyester were the major types of polymer compositions of MPs in long-horned beetles from Wuhan (39 % of total MP items) and Hangzhou (56 %), respectively. To our knowledge, this is the first study investigating the occurrence of MPs in wild terrestrial insects. These data are important for evaluating the risks of exposure to MPs for long-horned beetles.

Microplastics in Seawater, Sediment, and Organisms from Hangzhou Bay.

Microplastics (MPs) are widely present in global oceans, and can pose a threat to marine organisms. This study examined the abundance and characteristics of MPs in seawater, sediment, and organism samples collected from Hangzhou Bay. Abundance of MPs in seawater (n = 26) and sediment (n = 26) were 0.77-9.6 items/m3 and 44-208 items/kg dw, respectively. Size of MPs in sediment (mean 2.5 mm, range 0.21-5.3 mm) was significantly (p < 0.05) larger than that in seawater (1.1 mm, 0.13-4.9 mm). Fiber was consistently the predominant shape of MPs in seawater and sediment. The major polymer composition of MPs was polyethylene (PE; mean 47 %) in seawater, but textile cellulose (60 %) was the main polymer type of MPs in sediment. Average abundance of MPs in marine organisms (n = 388) ranged from 0.064 (zooplankton) to 2.9 (Harpodon nehereus) items/ind, with the mean size of 0.19-1.4 mm. MP abundance in marine organisms was not significantly correlated with their trophic level. Fiber was always the predominant shape of MPs in different marine organisms, contributing mean 67 (fish)-93 % (zooplankton) of total MPs. MPs in crustacean (mean 58 %), shellfish (64 %), and cephalopod (29 %) were dominated by textile cellulose. Whereas, PE (mean 44 %) and polypropylene (43 %) were the major polymer compositions of MPs in fish and zooplankton, respectively. To our knowledge, this is the most comprehensive study investigating the occurrence of MPs in environmental matrixes from Hangzhou Bay, which contributes to the better understanding of environmental behaviors of MPs in estuarine sea environment.

Microplastics in dust from different indoor environments.

Microplastics (MPs) are present in global indoor dust, which is an important source of MPs for humans. However, few researchers have investigated differences in the abundance and characteristics of MPs in dust in different indoor environments. In this study, we found that residential apartments (mean: 1174 MPs/g; n = 47) had the highest abundance of MPs in indoor dust samples, followed by offices (896 MPs/g; n = 50), business hotels (843 MPs/g; n = 53), university dormitories (775 MPs/g; n = 48), and university classrooms (209 MPs/g; n = 44). The predominant shape of MPs was fiber in most indoor dust samples. The main size fraction of the MPs in the indoor dust samples from university classrooms and business hotels was 201-500 µm, and it was 501-1000 µm in those from offices, university dormitories, and residential apartments. The main MP polymer in indoor dust samples from business hotels, university dormitories, and residential apartments was polyester, whereas those from offices and university classrooms were mainly polyethylene and polypropylene. We calculated the estimated daily intake (EDI) of MPs through the inhalation of indoor dust, and found that infants (7.4 MPs/kg bw/day) had a higher mean EDI of MPs than toddlers (1.4 MPs/kg bw/day), children (0.49 MPs/kg bw/day), adults (0.23 MPs/kg bw/day), and university students (0.22 MPs/kg bw/day). To the best of our knowledge, we are the first to report differences in MP occurrence in dust samples from different indoor environments, and our findings provide a more accurate understanding of exposure risks of MPs to humans.

Absorption, distribution, metabolism, excretion and toxicity of microplastics in the human body and health implications.

Microplastics (MPs; <5 mm) in the biosphere draws public concern about their potential health impacts. Humans are potentially exposed to MPs via ingestion, inhalation, and dermal contact. Ingestion and inhalation are the two major exposure pathways. An adult may consume approximately 5.1 × 103 items from table salts and up to 4.1 × 104 items via drinking water annually. Meanwhile, MP inhalation intake ranges from 0.9 × 104 to 7.9 × 104 items per year. The intake of MPs would be further distributed in different tissues and organs of humans depending on their sizes. The excretion has been discussed with the possible clearance ways (e.g., urine and feces). The review summarized the absorption, distribution, metabolic toxicity and excretion of MPs together with the attached chemicals. Moreover, the potential implications on humans are also discussed from in vitro and in vivo studies, and connecting the relationship between the physicochemical properties and the potential risks. This review will contribute to a better understanding of MPs as culprits and/or vectors linking to potential human health hazards, which will help outline the promising areas for further revealing the possible toxicity pathways.