RESUMO
This study proposes a new composite scaffold system. A woven polyethylenterephtalate (PET) fabric was coated on one side with a biodegradable PLGA film, in order to obtain a geometrically polarized scaffold structure for an bioartificial liver support system. The composite structure ensures the stability of the membrane during degradation of the membrane polymer. The mesh size of the composite does not significantly influence the degradation behavior. Hepatocyte culturing studies reveal that the formation of aggregates depends on the mesh size and on the pretreatment: The largest aggregates could be observed after 48 h when PVLA coating, large mesh size and EGF were combined. Thus, the combination of a geometrically structured, partially degradable scaffold with receptor-mediated cell attachment sites offers promising possibilities in liver tissue engineering.
Assuntos
Materiais Biocompatíveis/química , Engenharia Biomédica , Ácido Láctico/química , Fígado/fisiologia , Polietilenotereftalatos/química , Ácido Poliglicólico/química , Polímeros/química , Animais , Sítios de Ligação , Células Cultivadas , Estabilidade de Medicamentos , Fator de Crescimento Epidérmico/química , Lactose/análogos & derivados , Lactose/química , Microscopia Eletrônica de Varredura , Copolímero de Ácido Poliláctico e Ácido Poliglicólico , Poliestirenos/química , Fatores de TempoRESUMO
In this study, a composite scaffold combining textile superstructures and biomimetic glycopolymers is introduced, which may allow engineering of organotypic liver tissue in vitro. Woven poly(ethylene therephtalat) (PET) fabrics were coated on one side with a thin biodegradable polymer film (poly[D-L-lactic-co-glycolic acid] PLGA), in order to obtain a polar structure. The composite structure ensured the stability of the membrane during in vitro degradation, independently of mesh size. Matrix porosity increased when a polymer blend matrix was used. For hepatocyte culturing studies, the scaffolds were additionally coated with an artificial glycopolymer (poly[N-p-vinylbenzyl-D-lactoamide], PVLA) in order to improve cell attachment. It was observed that formation of aggregates depends on the scaffold geometry as well as on the pretreatment and medium conditions. After 4 days in culture, the pores of the fabric were filled with aggregates illustrating the possibility of immobilizing hepatocyte aggregates in well-defined spatial configurations on textile structures.