European Organization for Caries Research Workshop: Methodology for Determination of Potentially Available Fluoride in Toothpastes.

Toothpastes are the most universally accepted form of fluoride delivery for caries prevention. To provide anti-caries benefits, they must be able to release fluoride during the time of tooth brushing or post brushing into the oral cavity. However, there is no standard accepted procedure to measure how much fluoride in a toothpaste may be (bio) available for release. The European Organization for Caries Research proposed and supported a workshop with experts in fluoride analysis in toothpastes and representatives from industry. The objective of the workshop was to discuss issues surrounding fluoride analysis in toothpaste and reach consensus on terminology and best practices, wherever the available evidence allowed it. Participants received a background paper and heard presentations followed by structured discussion to define the problem. The group also reviewed evidence on the validity, reliability and feasibility of each technique (namely chromatography and fluoride electroanalysis) and discussed their strengths and limitations. Participants were able to reach a consensus on terminology and were also able to identify and summarize the advantages and disadvantages of each technique. However, they agreed that most currently available methods were developed for regulatory agencies several decades ago, utilizing the best available data from clinical trials then, but require to be updated. They also agreed that although significant advances to our understanding of the mechanism of action of fluoride in toothpaste have been achieved over the past 4 decades, this clearly is an extraordinarily complex subject and more work remains to be done.

Correlation between polymerization shrinkage and marginal fit of temporary crowns.

OBJECTIVES: During polymerization, temporary c&b materials (t-c&b) undergo shrinkage which may lead to marginal inaccuracies of the temporary restoration. The degree of correlation between these two factors is still unknown. Hence, the objective of this study was to determine the polymerization shrinkage of t-c&bs and to evaluate, to which degree the shrinkage is correlated to the width of the marginal gap. METHODS: Six different t-c&bs (2 monomethacrylates and 4 dimethacrylates) were used to fabricate temporary crowns (n = 12) on two prepared teeth (stainless steel) of different diameters (5 and 7 mm). The crown diameters and marginal discrepancy were measured at various storage times after mixing (10, 30, 60 min) using a travelling microscope. In addition, the shrinkage (bonded-disk method) and content of inorganic filler (ashing method) were recorded. The statistical analysis was carried out using parametric tests, the F-test and Pearson's correlation (p = 0.05). RESULTS: The marginal discrepancies increased as a function of increasing storage time (10 min: 0.162-0.218 mm; 60 min: 0.271-0.521 mm). Lower values were recorded for monomethacrylates than for the dimethacrylates (greatest changes recorded during the first 30 min). The shrinkage values recorded 10 min after mixing varied between 3.25 and 4.10%. There was no significant relationship between the width of the marginal gap and shrinkage (p > 0.05). SIGNIFICANCE: Shrinkage values are not suitable to predict the marginal accuracy of a temporary restoration. At least 30 min should elapse between fabricating and trimming temporary crowns to avoid further inaccuracies.

TGFB3 displays parent-of-origin effects among central Europeans with nonsyndromic cleft lip and palate.

Mice with a deletion of Tgf-beta3 (-/-) and association studies in humans of different ethnicities support the involvement of TGFB3 in the etiology of orofacial clefts. In this study, we investigated the relevance of TGFB3 in the development of cleft lip and palate (CL/P) among 204 triads of central European origin. Transmission-disequilibrium test (TDT) analysis revealed no significant transmission distortions for each marker alone, and none for any possible haplotypes. However, we found strong evidence for parent-of-origin effects, with lower risk of maternal transmission compared with paternal transmission [I (M) = 0.38; confidence interval (CI): 0.17-0.86] of the risk allele T to an affected offspring at marker rs2300607. This is also expressed in an increased risk of heterozygous children having the T allele inherited from the father (R (P) = 3.47; CI: 1.32-9.11). Our data support the involvement of TGFB3 in the development of oral clefts in patients of central European origin.

Recognition and modulation of cytochrome c's redox properties using an amphiphilic homopolymer.

An amphiphilic homopolymer scaffold has been used to bind to the protein, cytochrome c. This interaction is analyzed using cyclic voltammetry, native gel electrophoresis, UV-visible absorption, and circular dichroism spectroscopy. The polymer binds to cytochrome c with micromolar affinity and the association of polymer with cytochrome c leads to a structural change of the protein. This conformational change exposes the heme unit of the protein, which affords an opportunity to reversibly modulate its electron-transfer properties. We have also shown that the electrostatic binding of polymer to cytochrome c can be used to disrupt its interaction with its natural partner, cytochrome c peroxidase.