RESUMO
Hierarchical solution self-assembly has become an important biomimetic method to prepare highly complex and multifunctional supramolecular structures. However, despite great progress, it is still highly challenging to prepare hierarchical self-assemblies on a large scale because the self-assembly processes are generally performed at high dilution. Now, an emulsion-assisted polymerization-induced self-assembly (EAPISA) method with the advantages of inâ situ self-assembly, scalable preparation, and facile functionalization was used to prepare hierarchical multiscale sea urchin-like aggregates (SUAs). The obtained SUAs from amphiphilic alternating copolymers have a micrometer-sized rattan ball-like capsule (RBC) acting as the hollow core body and radiating nanotubes tens of micrometers in length as the hollow spines. They can capture model proteins effectively at an ultra-low concentration (ca. 10â nm) after functionalization with amino groups through click copolymerization.
Assuntos
Emulsões/química , Polímeros/química , Animais , Materiais Biomiméticos/química , Materiais Biomiméticos/metabolismo , Bovinos , Química Click , Compostos de Epóxi/química , Fluoresceína-5-Isotiocianato/química , Microscopia Eletrônica de Varredura , Nanotubos/química , Polimerização , Soroalbumina Bovina/química , Soroalbumina Bovina/metabolismoRESUMO
A class of cationic supramolecular block copolymers with readily controlled charges has been exploited. Upon post-synthetic structural optimization, this copolymer exhibits comparable biocompatibility, greatly improved pDNA condensation capability and biostability, and further enhanced transfection efficiency in vitro. This work provides valuable insight into the creation of advanced nonviral vectors for gene delivery.