RESUMO
Polyaniline (PANi) films were prepared by direct polymerizing deposition with four different kinds of acids as dopants or were prepared by a casting method on the surface of a polytetrafluoroethylene substrate. The properties of PANi films were characterized using atomic force microscopy, electrical conductivity measurements, and water contact angle measurements. Unlike the casting PANi film, experimental results indicated that the synthesized PANi films had a similar nanostructure as that of average nanoparticles (approximate diameter of 30-50 nm). To investigate the potential usefulness of PANi films in biomedical applications, we also studied their biocompatibility through the adhesion and proliferation properties of PC-12 pheochromocytoma cells. All the films were found to be biocompatible and allowed cell attachment and proliferation. However, the synthesized films have a much higher ability for cell adhesion than the casting film. After 4 days of culture on different PANi films, the cells formed more confluent monolayers on the synthesized PANi films than on the casting films. These results demonstrate that the PANi films could be used to culture neurotic cells and that their surface architecture on the nanoscale may affect cell function such as attachment and proliferation.
Assuntos
Ácidos Acíclicos/química , Ácidos não Carboxílicos/química , Compostos de Anilina/química , Materiais Biocompatíveis/química , Nanoestruturas/química , Alicerces Teciduais/química , Compostos de Anilina/síntese química , Animais , Materiais Biocompatíveis/síntese química , Adesão Celular , Proliferação de Células , Forma Celular , Ácido Cítrico/química , Condutividade Elétrica , Ácido Clorídrico/química , Interações Hidrofóbicas e Hidrofílicas , Malatos/química , Microscopia de Força Atômica , Microscopia Eletrônica de Varredura , Nanopartículas/química , Células PC12 , Tamanho da Partícula , Percloratos/química , Politetrafluoretileno/química , Ratos , Propriedades de Superfície , MolhabilidadeRESUMO
High quality films of polythiophene with different alkyl side chains were successfully synthesized by a novel method in the presence of sodium dodecylbenzenesulonate (SDBS) under N2 atmosphere on the PTFE (polytetrafluorethylene) substrate. The as-prepared films were characterized by scanning electron microscopy (SEM), conductivity measurement, and water contact angle measurement. The morphologies of the films were homogeneous with micro-/nanostructures, and their conductivities were high enough for biomedical applications. Hydrophobicity of the films could be adjusted easily by inducing alkyl side chains with different length, which could control protein adsorption in succession. Hydrophobic polythiophene film with a long alkyl side chain had a higher ability of protein adsorption and PC 12 cell proliferation. The biocompatibility study of the synthesized films in vitro proved that the synthesized films were not cytotoxic to two cell lines used and could support cell attachment and proliferation well. Polythiophenes films prepared by in-situ deposition will be good candidates for biomedical applications.