Enzyme-free quantification of exosomal microRNA by the target-triggered assembly of the polymer DNAzyme nanostructure.

We herein report an efficient hybridization chain reaction (HCR)- and DNAzyme-based enzyme-free signal amplification for the detection of specific exosomal miRNAs in the culture medium of cancer cells and serum samples from cancer patients via the target-triggered self-assembly of the polymer DNAzyme nanostructure.

Temperature responsive fluorescent polymer nanoparticles (TRFNPs) for cellular imaging and controlled releasing of drug to living cells.

Recent studies have demonstrated that drug delivery by using functional nanomaterials with imaging capability could afford plenty of insightful information for the better control of the delivery process. In this work, we developed temperature responsive fluorescent nanoparticles (TRFNPs) for drug delivery and cellular imaging. The TRFNP was fabricated by one-pot co-precipitation of thermal sensitive amphiphilic block copolymers polystyrene-b-poly(N-isopropyl acrylamide) (PS-b-PNIPAM) and fluorescent conjugated polymer poly [(9,9-dioctylfluorenyl-2,7-diyl)-alt-co-(1,4-benzo(2,1',3)-thiadiazole)] (PFBT) in the presence of desired small guest molecules. The dynamic light scattering (DLS) measurements verified that this functional nanoparticle exhibited temperature dependent size variation, which could therefore regulate the releasing rate of loaded guest molecules (e.g. drugs) inside the polymer core. Besides, the TRFNPs displayed good photostability in terms of optical characterization. The cellular cytotoxicity characterization demonstrated that this nanoparticle exhibited good biocompatibility even under the mass concentration of 10µg/mL. By using Nile Red as a model molecule, the temperature-controlled releasing process from TRFNPs in solution as well as inside living cells was monitored directly according to the spectroscopic and microscopic characterizations. Furthermore, anti-cancer drug was successfully delivered into living cells via TRFNPs and released in a temperature dependent manner. As a consequence, owing the attractive merits as mentioned above, this nanostructure would find broad applications in nanomedicine in the future.