RESUMO
Water soluble NaYF4 nanocrystals codoped with 20 mol% Yb3+, 2 mol% Er3+ were prepared by a facile solvothermal approach using polyvinylpyrrolidone (PVP) as a surfactant. As a potential material for luminescent probes, in votroeffects of upconversion nanoparticles (UCNPs) on human aenocarcinoma (SGC-7901) cells with different concentrations were observed. These effects range from cell viability, mitochondrial membrane potential (MMP), reactive oxygen species (ROS) production, AnnexinV-FITC-propidium iodide (PI) apoptosis detection, and cell cycles. Our results demonstrated that the cells treated with UCNPs showed a decrease in cell viability accompanied the decreased MMP and the release of ROS. When treated with 400 µg/mL UCNPs, AnnexinV-FITC-PI apoptosis detection showed the UCNPs induced apoptosis, the cell cycle indicated the UCNPs suppressor cells in the G1 phase obviously, thereby reducing cell activity.
Assuntos
Sobrevivência Celular/efeitos dos fármacos , Fluoretos/química , Fluoretos/toxicidade , Nanopartículas Metálicas/química , Nanopartículas Metálicas/toxicidade , Ítrio/química , Ítrio/toxicidade , Materiais Biocompatíveis , Érbio/química , Érbio/toxicidade , Teste de Materiais , Nanopartículas Metálicas/ultraestrutura , Solubilidade , Água/química , Itérbio/química , Itérbio/toxicidadeRESUMO
We investigated the cellular uptake behavior and cell viability of semiconducting polymer dots (Pdots) on human gastric adenocarcinoma (SGC-7901) cells and human gastric mucosal (GES-1) cells. MTT studies indicate the Pdot treatment induces obvious cell proliferation in both types of cell lines. We performed further investigations such as reactive oxygen species (ROS) generation and mitochondrial membrane potential (MMP) change, which indicate that the cell proliferation is in good agreement with the increase in the ROS and MMP levels. Moreover, expression of protein kinase B (Akt) decreased as the Pdot concentration increases, but the expression of protein dual-phosphorylated Erk (p-Erk) and phosphorylated c-Jun N-terminal kinases (p-JNK) were increased. These effects indicated that the Pdots could promote the growth of SGC-7901 cells and GES-1 cells by appropriately regulating the expressions of protein Akt, p-Erk, and p-JNK.
Assuntos
Adenocarcinoma/patologia , Mucosa Gástrica/patologia , Polímeros/farmacologia , Semicondutores , Neoplasias Gástricas/patologia , Ciclo Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Citometria de Fluxo , Fluorescência , Mucosa Gástrica/efeitos dos fármacos , Humanos , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Imagem Molecular , Proteínas de Neoplasias/metabolismo , Espécies Reativas de Oxigênio/metabolismoRESUMO
In this work, a simple, low-cost and one-step microwave approach has been demonstrated for the synthesis of water-soluble carbon dots (C-dots). The average size of the resulting C-dots is about 4 nm. From the photoluminescence (PL) measurements, the C-dots exhibit excellent biocompatibility and intense PL with the high quantum yield (QY) at Ca. 25%. Significantly, the C-dots have excellent biocompatibility and the capacity to specifically target the cells overexpressing the folate receptor (FR). These exciting results indicate the as-prepared C-dots are promising biocompatible probe for cancer diagnosis and treatment.