[Sjögren's syndrome combined with cold agglutinin disease: A case report].

Sjögren's syndrome(SS)is a chronic autoimmune disease that affects exocrine glands, especially salivary and lacrimal glands. The main clinical manifestations are dry mouth and dry eyes, but also multi-organ and multi-system can be involved. Cold agglutinin disease(CAD)is an autoimmune disease characterized by red blood cell agglutination in the blood vessels of extremities caused by cold agglutinin at low temperature, resulting in skin microcirculation disturbance, or hemolytic anemia. Cold agglutinin disease is divided into two categories, primary cold agglutinin disease and secondary cold agglutinin disease. Primary cold agglutinin disease is characterized with cold agglutinin titer of 1 ∶4 000 or more and positive Coomb's test. However, the Coomb's test is not necessarily positive and the cold agglutinin titer is between 1 ∶32 and 1 ∶4 000 in secondary cold agglutinin disease. Here, we reported an elderly patient admitted to hospital due to fever. He was diagnosed with respiratory infection, but he showed incompletely response to the anti-infection treatment. Further laboratory tests showed the patient with positive ANA and anti-SSA antibodies. Additionally, the patient complained that he had dry mouth and dry eyes for 1 year. Schirmer test and salivate gland imaging finally confirmed the diagnosis Sjogren's syndrome. During the hospital stay, the blood clots were found in the anticoagulant tubes. Hemolytic anemia was considered as the patient had anemia with elevated reticulocytes and indirect bilirubin. In addition, further examination showed positive cold agglutination test with a titer of 1 ∶1 024, and cold agglutinin disease was an important type of cold-resistant autoimmune hemolytic anemia. Furthermore, the patient developed cyanosis after ice incubating at the tip of the nose. Hence, the patient was diagnosed as CAD and he was successfully treated with glucocorticoids instead of anti-infection treatments. Hence, the patient was diagnosed with SS combined with secondary CAD. SS combined CAD are rarely reported, and they are both autoimmune diseases. The abnormal function of B lymphocytes and the production of autoantibodies might be the common pathogenesis of them. Cold agglutinin disease can lead to severe hemolytic anemia, even life-threatening. In clinical practice, timely recognizing and dealing with CAD might promote the prognosis of the patient.

Construction of actinomycetes complex flora in degrading corn straw and an evaluation of their degradative effects.

OBJECTIVES: As a type of agricultural waste, there is a large amount of lignocellulose in corn (Zea mays) straw, but it is difficult to utilize efficiently owing to its recalcitrance to enzymatic degradation. Three strains of actinomycetes that degrade cellulose were constructed as complex flora, and the conditions of cellulose degradation conditions and their degradative activity were optimized and evaluated. RESULTS: When the complex flora were inoculated into the fermentation medium at pH 7 and 3% (v/v), the rate of degradation of corn straw reached 38.24% after 5 d of fermentation at 28 ºC and 180 rpm. Cellulose, hemicellulose, and lignin in the corn straw were degraded by 33.97%, 34.08%, and 21.52%, respectively. The results from scanning electron microscopy showed that the waxy layer on the surface of corn straw became thin and gradually disappeared following fermentation by the complex flora. Fourier-transform infrared spectroscopy showed that the complex flora could change the internal functional groups of corn straw at different fermentation periods. The compounds detected in the fermentation system indicated that the corn straw was efficiently degraded. CONCLUSIONS: These results indicated that the constructed complex flora was more effective at degrading corn straw than the individual strains and provides research concepts for the development and utilization of biomass resources.

The screening and validation process of miR-223-3p for saliva identification.

More accurate identification of the types of body fluids left at a crime scene is indispensable for improving the judicial chain of evidence. MicroRNAs (miRNAs) have become recognized as ideal molecular markers for the identification of body fluids in forensic science due to their short length, stability and high tissue specificity. In this study, small RNA sequencing was performed on 20 samples of five types of body fluids (peripheral blood, menstrual blood, saliva, semen, and vaginal secretions) with the BGISEQ-500 sequencing platform, and the specific miRNA markers of saliva and vaginal secretions were screened by bioinformatics methods, including differential expression analysis and significant enrichment analysis. Through RT-qPCR validation of 169 samples, we confirmed that miR-223-3p can be used as a saliva-specific marker. In addition, we considered miR-223-3p in combination with four other miRNA molecules (miR-451a, miR-891a-5p, miR-144-5p, miR-203a-3p) that had been previously screened and verified in our laboratory, and seven body fluid prediction models based on machine learning algorithms were constructed and verified. The results showed that a kernel density estimation (KDE) model based on the five miRNA markers for body fluid identification could achieve 100% accuracy in the samples tested in the present study.

Identification and Characterization of Mercury Contamination in Vegetables and Herbs Cultivated on a Commercial Vertical Indoor Farming System with Light-Emitting Diode Lighting: Unveiling an Unusual Food Safety Risk of Some Improperly Manufactured High-Density Agricultural Production Systems.

Artificial grow lights, such as light-emitting diodes (LEDs) and fluorescent grow lights, are commonly used in modern day indoor farming, citing advantages in energy efficiency and a higher controlled environment. However, the use of LEDs poses a risk in mercury contaminations as a result of its production process, specifically LEDs with polyurethane encapsulates that were traditionally produced using mercury resins as a catalyst. A total of 10.0 ppm of mercury was detected in a curly kale sample harvested from an indoor hydroponic vegetable farm, exceeding Singapore Food Regulation's limit of 0.05 ppm. Vegetables, farming inputs, and surface swabs from the affected farm were analyzed using wet acid digestion followed by cold vapor atomic absorption spectroscopy analysis. The investigation found high concentrations of mercury in the LED encapsulant, and the encapsulant material was identified to be polyurethane by Fourier transform infrared spectroscopy and pyrolysis-gas chromatography-mass spectrometry analysis, indicating the source of mercury contamination to be the LED polyurethane encapsulant.

Preparation, characterization, and in vivo evaluation of levonorgestrel-loaded thermostable microneedles.

To facilitate the storage and use of poly (lactic-co-glycolic acid) (PLGA)-based microneedles (MNs) in hot seasons and regions, thermally stable MNs loaded with levonorgestrel (LNG) were developed. Due to its good biocompatibility and high glass transition temperature (Tg), Hydroxypropyl methylcellulose (HPMC) was added to the PLGA-based MNs to increase thermal stability. MNs with HPMC exhibited excellent thermal stability at high temperatures. After the MNs has been applied to the skin for 10 min, the backing layer of the MNs was dissolved by contact with the interstitial fluid of skin, which resulted in the separation of the MN tips from the backing layer. The MN tips were implanted intradermally and sustained-release LNG. Biodegradable polymers were used to encapsulate the LNG, providing long-acting contraception. The in vitro release rate of LNG from the MNs reached 72.78%-83.76% within 21 days. In rats, the MNs maintained plasma concentrations of LNG above the human contraceptive level for 8-12 days. In mice, the time required for complete degradation of the MN tips was 12-16 days. MNs have excellent medication adherence due to the advantages of painlessness, minimally invasive, and self-administered. MNs can make long-acting contraceptives more readily available to humans.

Tumor RNA-loaded nanoliposomes increases the anti-tumor immune response in colorectal cancer.

PURPOSE: Tumor RNA vaccines can activate dendritic cells to generate systemic anti-tumor immune response. However, due to easily degraded of RNA, direct RNA vaccine is less effective. In this study, we optimized the method for preparing PEGylated liposom-polycationic DNA complex (LPD) nanoliposomes, increased encapsulate amount of total RNA derived from CT-26 colorectal cancer cells. Tumor RNA LPD nanoliposomes vaccines improved anti-tumor immune response ability of tumor RNA and can effectively promote anti-tumor therapeutic effect of oxaliplatin. METHODS: Total tumor-derived RNA was extracted from colorectal cancer cells (CT-26 cells), and loaded to our optimized the LPD complex, resulting in the LPD nanoliposomes. We evaluated the characteristics (size, zeta potential, and stability), cytotoxicity, transfection ability, and tumor-growth inhibitory efficacy of LPD nanoliposomes. RESULTS: The improved LPD nanoliposomes exhibited a spherical shape, RNA loading efficiency of 9.07%, the average size of 120.37 ± 2.949 nm and zeta potential was 3.34 ± 0.056 mV. Also, the improved LPD nanoliposomes showed high stability at 4 °C, with a low toxicity and high cell transfection efficacy toward CT-26 colorectal cancer cells. Notably, the improved LPD nanoliposomes showed tumor growth inhibition by activating anti-tumor immune response in CT-26 colorectal cancer bearing mice, with mini side effects toward the normal organs of mice. Furthermore, the effect of the improved LPD nanoliposomes in combination with oxaliplatin can be better than that of oxaliplatin alone. CONCLUSION: The improved LPD nanoliposomes may serve as an effective vaccine to induce antitumor immunity, presenting a new treatment option for colorectal cancer.

Deeper insight into hydrolysis mechanisms of polyester/cotton blended fabrics for separation by explicit solvent models.

Aiming to get a deeper and accurate understanding on separation of polyester/cotton blended fabrics in subcritical water, the hydrolysis mechanisms of cellulose and polyester were studied using dispersion-corrected density functional theory (DFT-D) method with and without explicit H2O under the conductor-like screening model (COSMO) set. The number and locations of explicit H2O were determined by their likely functions including being dissociation and solvent and catalyst. The calculations disclosed that explicit H2O provide inductive activation on glycosidic bond of cellulose and ester groups at the center of polyester and the assistance on the transfer of proton as proton-carrier and as catalyst of proton shuttle, affecting the reaction and activation energies in a realistic manner. In addition, the number of explicit H2O molecules functioning as catalyst of proton shuttle may also has a strong influence on catalytic activity. Based on the improved explicit solvation models, the overall activation energies of proposed hydrolysis mechanisms for cellulose and polyester are 14.81 and 21.46 kcal/mol respectively, which explains the preferential hydrolysis of cellulose from experimental results.

A supramolecular structure insight for conversion property of cellulose in hot compressed water: Polymorphs and hydrogen bonds changes.

Waste paper samples with different cellulose supramolecular structure were treated in hot compressed water (HCW) at 375°C and 22.5MPa within 200s to evaluate the specific effect mechanism of cellulose supramolecular structure on the conversion of waste paper to reusable resource. Although the distribution of liquid products and the oligosaccharides were related to reaction time, depolymerization and decrystallization of the cellulose, the characteristics absorption peak of cellulose from FTIR analysis and crystal structure of the cellulose detected in the residues with hydrolysis rate up 96.5% indicated crystal structure was the dominant factor that affect conversion behavior of waste paper. The conversion of cellulose Iß to cellulose Iα or cellulose I(α+ß) in HCW demonstrated that the recrystallization occurred during the decrystallization of cellulose through the rearrangement of hydrogen bonds.

Studies on the development of latent fingerprints by the method of solid-medium ninhydrin.

A new series of fingerprint developing membrane were prepared using ninhydrin as the developing agent, and pressure-sensitive emulsifiers as the encapsulated chemicals. The type of emulsifier, plastic film, concentration of the developing agent, modifying ions and thickness of the membrane were studied in order to get the optimized fingerprint developing effect. The membrane can be successfully applied to both latent sweat fingerprints and blood fingerprint on many different surfaces. The sensitivity of the method toward the latent sweat fingerprint is 0.1 mg/L amino acid. The membrane can be applied to both porous and non-porous surfaces. Fingerprints that are difficult to develop on surfaces such as leather, glass and heat-sensitive paper using traditional chemical methods can be successfully developed with this membrane.