The construction of elastin-like polypeptides and their applications in drug delivery system and tissue repair.

Elastin-like polypeptides (ELPs) are thermally responsive biopolymers derived from natural elastin. These peptides have a low critical solution temperature phase behavior and can be used to prepare stimuli-responsive biomaterials. Through genetic engineering, biomaterials prepared from ELPs can have unique and customizable properties. By adjusting the amino acid sequence and length of ELPs, nanostructures, such as micelles and nanofibers, can be formed. Correspondingly, ELPs have been used for improving the stability and prolonging drug-release time. Furthermore, ELPs have widespread use in tissue repair due to their biocompatibility and biodegradability. Here, this review summarizes the basic property composition of ELPs and the methods for modulating their phase transition properties, discusses the application of drug delivery system and tissue repair and clarifies the current challenges and future directions of ELPs in applications.

Effect of LSD1 on osteogenic differentiation of human periodontal ligament stem cells in periodontitis.

OBJECTIVE: Periodontitis is a chronic inflammation of periodontal tissues. This study is expected to assess the effect of LSD1 on the osteogenic differentiation of hPDLSCs in periodontitis. METHODS: hPDLSCs were separated, cultivated, and identified, and then treated by LPS to induce inflammatory microenvironment and subjected to osteogenic differentiation. Subsequently, LSD1 expression was determined, and then silenced to assess its effect on hPDLSCs. Next, the binding relation between LSD1 and miR-590-3p was analyzed. miR-590-3p expression was detected and then overexpressed to evaluate its role in hPDLSCs in periodontitis. Afterward, the relation between LSD1 and OSX was analyzed. H3K4me2 level and OSX transcription were measured, and the role of H3K4me2 was determined. Additionally, the role of OSX in hPDLSCs was verified. RESULTS: LSD1 was poorly expressed after osteogenic differentiation of hPDLSCs while it was rescued upon LPS induction. The osteogenic differentiation of hPDLSC in periodontitis was strengthened upon LSD1 downregulation. Besides, miR-590-3p targeted LSD1 transcription, and LSD1 inhibited OSX transcription via H3K4me2 demethylation. miR-590-3p overexpression improved osteogenic differentiation of hPDLSCs in periodontitis. But this improvement was annulled by OSX inhibition. CONCLUSION: miR-590-3p targeted LSD1 transcription and upregulated H3K4me2 methylation to promote OSX transcription, thereby encouraging osteogenic differentiation of hPDLSCs in periodontitis.

Recent advances in the research on effects of micro/nanoplastics on carbon conversion and carbon cycle: A review.

Massive production and spread application of plastics have led to the accumulation of numerous plastics in the global environment so that the proportion of carbon storage in these polymers also increases. Carbon cycle is of fundamental significance to global climate change and human survival and development. With the continuous increase of microplastics, undoubtedly, there carbons will continue to be introduced into the global carbon cycle. In this paper, the impact of microplastics on microorganisms involved in carbon transformation is reviewed. Micro/nanoplastics affect carbon conversion and carbon cycle by interfering with biological fixation of CO2, microbial structure and community, functional enzymes activity, the expression of related genes, and the change of local environment. Micro/nanoplastic abundance, concentration and size could significantly lead to difference in carbon conversion. In addition, plastic pollution can further affect the blue carbon ecosystem reduce its ability to store CO2 and marine carbon fixation capacity. Nevertheless, problematically, limited information is seriously insufficient in understanding the relevant mechanisms. Accordingly, it is required to further explore the effect of micro/nanoplastics and derived organic carbon on carbon cycle under multiple impacts. Under the influence of global change, migration and transformation of these carbon substances may cause new ecological and environmental problems. Additionally, the relationship between plastic pollution and blue carbon ecosystem and global climate change should be timely established. This work provides a better perspective for the follow-up study of the impact of micro/nanoplastics on carbon cycle.

Submental Island Flap Combined With Reconstruction Titanium Plate: A New Way to Treat Mandibular Osteoradionecrosis.

Objective. The purpose of this study was to investigate the effect of reconstruction for bone defects in patients with mandibular osteoradionecrosis using submental flap combined with reconstructive titanium plate. Methods. A total of 23 patients with mandibular osteoradionecrosis were included. All patients underwent surgical resection and immediate reconstruction with submental flap combined with reconstructive titanium plate. The postoperative effects and flap success rate were evaluated with a 6- to 18-month follow-up. Results. Twenty-two flaps were successful; 1 flap suffered from partial necrosis in the distal end, but the patient was treated with hyperbaric oxygen therapy and iodoform gauze dressing, and no plate exposure was found after operation in the follow-up period. Conclusion. The use of submental flap combined with reconstructive titanium plate for treating the patients with osteoradionecrosis is a feasible, cost-effective, simple, and safe procedure.

[A tooth cone beam computer tomography image segmentation method based on the local Gaussian distribution fitting].

Oral teeth image segmentation plays an important role in teeth orthodontic surgery and implant surgery. As the tooth roots are often surrounded by the alveolar, the molar's structure is complex and the inner pulp chamber usually exists in tooth, it is easy to over-segment or lead to inner edges in teeth segmentation process. In order to further improve the segmentation accuracy, a segmentation algorithm based on local Gaussian distribution fitting and edge detection is proposed to solve the above problems. This algorithm combines the local pixels' variance and mean values, which improves the algorithm's robustness by incorporating the gradient information. In the experiment, the root is segmented precisely in cone beam computed tomography (CBCT) teeth images. Segmentation results by the proposed algorithm are then compared with the classical algorithms' results. The comparison results show that the proposed method can distinguish the root and alveolar around the root. In addition, the split molars can be segmented accurately and there are no inner contours around the pulp chamber.

Albiflorin Inhibits Advanced Glycation End Products-Induced ROS and MMP-1 Expression in Gingival Fibroblasts.

BACKGROUND: Periodontitis is a common complication of diabetes, with advanced glycation end products (AGEs) playing a key role in its pathogenesis. Albiflorin, a monoterpene glycoside, has shown potential anti-inflammatory and antioxidant properties. This study aims to investigate the effects of albiflorin on AGEs-induced gingival fibroblasts and its underlying mechanisms. OBJECTIVE: This study aimed to evaluate the role of albiflorin in mitigating ROS production, inflammation, and MMP-1 expression in AGEs-induced gingival fibroblasts. METHODS: The viability of gingival fibroblasts treated with albiflorin and AGEs was assessed using CCK-8 assays. ROS levels were measured by DCF staining, and the expression of inflammatory markers and MMP-1 was evaluated by ELISA and qPCR. The involvement of the NF-κB and Nrf2 pathways was examined by immunoblotting. RESULTS: Albiflorin enhanced the viability of AGEs-induced gingival fibroblasts and reduced ROS production. It also decreased the expression of IL-6, IL-8, RAGE, and MMP-1, suggesting an anti- inflammatory effect. Mechanistically, albiflorin modulated the NF-κB and Nrf2 pathways in AGEs-induced gingival fibroblasts. CONCLUSION: Albiflorin exhibited protective effects against AGEs-induced oxidative stress and inflammation in gingival fibroblasts, highlighting its potential as a therapeutic agent for periodontitis in diabetic patients. The modulation of the NF-κB and Nrf2 pathways by albiflorin provides insight into its mechanism of action.

Is the application of organic fertilizers becoming an undeniable source of microplastics and resistance genes in agricultural systems?

The application of organic fertilizers is becoming an undeniable source of microplastics and antibiotic resistance genes (ARGs) in agricultural soils. The complex microbial activity further transfers resistance genes and their host bacteria to agricultural products and throughout the entire food chain. Therefore, the current main focus is on reducing the abundance of microplastics and ARGs in organic fertilizers at the source, as well as managing microplastics and ARGs in soil. The control of microplastic abundance in organic fertilizers is currently only achieved through pre-composting selection and other methods. However, there are still many shortcomings in the research on the distribution characteristics, propagation and diffusion mechanisms, and control technologies of ARGs, and some key scientific issues still need to be urgently addressed. The high-temperature composting of organic waste can effectively reduce the abundance of ARGs in organic fertilizers to a certain extent. However, it is also important to consider the spread of ARGs in residual antibiotic-resistant bacteria (ARB). This article systematically explores the pathways and interactions of microplastics and resistance genes entering agricultural soils through the application of organic fertilizers. The removal of microplastics and ARGs from organic fertilizers was discussed in detail. Based on the limitations of existing research, further investigation in this area is expected to provide valuable insights for the development and practical implementation of technologies aimed at reducing soil microplastics and resistance genes.

Distribution and characteristics of microplastics in 16 benthic organisms in Haizhou Bay, China: Influence of habitat, feeding habits and trophic level.

Microplastics (MPs) are widely found in the ocean and cause a serious risk to marine organisms. However, fewer studies have been conducted on benthic organisms. This study conducted a case study on the pollution characteristics of MPs on 16 marine benthic organisms in Haizhou Bay, and analyzed the effects of habitat, trophic level, and feeding mode on the MPs pollution characters. The results showed that MPs were detected in all 16 organisms with an average abundance of 8.84 ± 9.14 items/individual, which is in the middle-high level in the international scale. Among the detected MPs, the main material was cellophane. This study showed that benthic organisms can be used as indicator organisms for MPs pollution. MPs in organisms can be affected by their habitat, trophic level, and feeding mode. Comprehensive analysis of MPs in benthic organisms will contribute to fully understand the characterization and source resolution of MPs pollution.

Can microplastics and disinfectant resistance genes pose conceivable threats to water disinfection process?

Microplastic pollution in the environment has aroused widespread concerns, however, the potential environmental risks caused by excessive use of disinfectants are still unknown. Disinfectants with doses below the threshold can enhance the communication of resistance genes in pathogenic microorganisms, promoting the development and spread of antimicrobial activity. Problematically, the intensification of microplastic pollution and the increase of disinfectant consumption will become a key driving force for the growth of disinfectant resistance bacteria (DRB) and disinfectant resistance genes (DRGs) in the environment. Disinfection plays a crucial role in ensuring water safety, however, the presence of microplastics and DRGs seriously disturb the water disinfection process. Microplastics can reduce the concentration of disinfectant in the local environment around microorganisms and improve their tolerance. Microorganisms can improve their resistance to disinfectants or generate resistance genes via phenotypic adaptation, gene mutations, and horizontal gene transfer. However, very limited information is available on the impact of DRB and DRGs on disinfection process. In this paper, the contribution of microplastics to the migration and transmission of DRGs was analyzed. The challenges posed by the presence of microplastics and DRGs on conventional disinfection were thoroughly discussed. The knowledge gaps faced by relevant current research and further research priorities have been proposed in order to provide a scientific basis in the future.

Are microplastics in aquaculture an undeniable driver in accelerating the spread of antibiotic resistance genes?

Aquaculture products have been a key source of protein in the human food supply. Contamination by microplastics and antibiotic resistance genes (ARGs) directly affects food quality and safety. Plastic fishing gear and the long-term misuse of antibiotics result in the persistent residue, migration, and spread of microplastics and ARGs in the aquaculture environment, causing in ecological imbalance and endangering human security. Microplastics can act as "petri dishes" for the reproduction, communication, and spread of ARGs, which adds an additional layer of complexity to the global issues surrounding microplastics and ARGs. Aquaculture has become an important source of microplastics and ARGs in natural waters. Accordingly, this paper mainly discusses the contribution of aquaculture to the presence of microplastics and ARGs in aquatic ecosystems. Microplastics and ARGs can (1) affect the production and quality of aquatic products; (2) influence the development and reproduction of aquatic organisms; and (3) accelerate the spread of resistant bacteria. How to eliminate microplastics and ARGs and block their transmission has become a worldwide problem. Actually, further research is required to understand the scale and scope of these effects.

Disulfide-Bridged Dendritic Organosilicas-Based Biodegradable Molecularly Imprinted Polymers for Multiple Targeting and pH/Redox-Responsive Drug Release toward Chemical/Photodynamic Synergistic Tumor Therapy.

In this study, a sialic acid (SA) and transferrin (TF) imprinted biodegradable disulfide bridging organosilicas-based drug delivery system (SS-DMONS/DOX-Ce6@MIPs) for targeted cancer therapy is constructed, for the first time. Disulfide bridged dendritic mesoporous organosilicas nanoparticles (SS-DMONs) not only enhance drug loading as the drug repository, but also provide enough specific surface area for the molecular imprinting shell to expose more degradation and imprinted sites on the surface. In addition, SS can be disturbed in a highly reducing tumor microenvironment to achieve degradation. The biodegradable imprinting film, prepared with customized 2-amino-N-(3,4-dihydroxyphenethyl)-3-mercaptopropanamide and 4-mercaptophenylboronic acid as functional monomers, endows SS-DMONs with active targeting capacity, and responsive drug release through degradation under acidic and highly reductive tumor microenvironment. SS-DMONS/DOX-Ce6@MIPs after binding of TF can target tumor cells actively through multiple interactions, including the affinity between antigen and antibody, and the specific recognition between molecularly imprinted polymers and template molecules. Under laser irradiation the loaded chlorin e6 (Ce6) that can produce toxic reactive oxygen, combined with the doxorubicin (DOX), achieves chemical/photodynamic synergistic anticancer effects. SS-DMONS/DOX-Ce6@MIPs present excellent tumor targeting and dual-responsive drug release, which provides an effective strategy for chemical/photodynamic antitumor therapy.

Are algae a promising ecofriendly approach to micro/nanoplastic remediation?

How to reduce microplastic pollution in aquatic ecosystem has become the focus of the global attention. The re-removal of microplastics of wastewater treatment plant (WWTP) effluent is gradually being put on the agenda. Recently, algae have been used as an ecofriendly remediation strategy for microplastic removal. Microplastics in sewage can be removed by algae through interception, capture, and entanglement, and can also form heterogeneous aggregates with algae, thereby reducing their free suspensions. Algae can recover nitrogen and carbon from wastewater and can be made into biochar, biofertilizers, and biofuels. However, problematically, this technology has been in the laboratory research stage, and existing research results cannot provide effective basis for its application. Microplastic removal via algae is influenced by wastewater flow rate, microplastic types, and pollutants. Microplastics are only physically fixed by algae, and ensuring that microplastics do not re-enter the environment during resource and capacity recovery is also a key factor limiting the implementation of this technology. The topic of this paper is to discuss the performance of the current tertiary wastewater treatment process - algae process to remove microplastics. Algae can remove nitrogen and phosphorus pollutants in sewage and remove microplastics at the same time, which can realize energy recovery and reduce ecological risks of the effluent. Although algae combined tertiary sewage treatment is a green technology for microplastic removal, its application still needs to be explored. The key challenges that need to be addressed, from single laboratory conditions to complex conditions, from small-scale testing to large-scale simulations, lie ahead of the application of this friendly technology.

Biodegradable Imprinted Polymer Based on ZIF-8/DOX-HA for Synergistically Targeting Prostate Cancer Cells and Controlled Drug Release with Multiple Responses.

Improving the drug loading and delivery efficiency of biodegradable nanomaterials used for targeting prostate cancer (PCa) remains a challenging task. To accomplish this task, herein, a new surface molecularly imprinted polymer (ZIF-8/DOX-HA@MIP) was designed and constructed with a hyaluronic acid (HA)-modified zeolitic imidazolate framework-8 (ZIF-8) metal-organic framework loaded with doxorubicin (DOX) as a substrate and a responsive molecularly imprinted polymer film as a shell. Owing to the large surface area of ZIF-8, DOX was successfully loaded into the ZIF-8/DOX-HA@MIP with a high drug loading efficiency (more than 88%). In vitro cell experiments have shown that the strengthened targeting ability of ZIF-8/DOX-HA@MIP to PCa cells was realized through the synergistic effect of HA and the molecularly imprinted membrane. Under the condition of simulated tumor microenvironment solution, Zn species were released and the particle size of ZIF-8/DOX-HA@MIP decreased gradually by the synergistic effect of hyaluronidase, pH, and glutathione, showing excellent biodegradability. In vivo antitumor research indicated the excellent antitumor activity and biocompatibility of ZIF-8/DOX-HA@MIP. The multifunctional ZIF-8/DOX-HA@MIP constructed herein provides a novel impetus for the development of targeted drug delivery in PCa treatment and a new strategy for treating other tumors.

Research advances on impacts micro/nanoplastics and their carried pollutants on algae in aquatic ecosystems: A review.

The widespread presence of micro/nanoplastics in aquatic ecosystems has certainly affected ecosystem functions and food chains/webs. The impact is worsened by the accumulation of different pollutants and microorganisms on the surface of microplastics. At the tissue, cellular, and molecular levels, micro/nanoplastics and the contaminants they carry can cause damage to aquatic organisms. Problematically, the toxic mechanism of micro/nanoplastics and contaminants on aquatic organisms is still not fully understood. Algae are key organisms in the aquatic ecosystem, serving as primary producers. The investigation of the toxic effects and mechanisms of micro/nanoparticles and pollutants on algae can contribute to understanding the impact on the aquatic ecosystem. Micro/nanoplastics inhibit algal growth, reduce chlorophyll and photosynthesis, induce ultrastructural changes, and affect gene expression in algae. The effects of energy flow can alter the productivity of aquatic organisms. The type, particle size, and concentration of micro/nanoparticles can influence their toxic effects on algae. Although there has been some research on the toxic effects of algae, the limited information has led to a significant lack of understanding of the underlying mechanisms. This paper provides a comprehensive review of the interactions between micro/nanoplastics, pollutants, and algae. The effects of various factors on algal toxicity are also analyzed. In addition, this article discusses the combined effects of microplastics, global warming, and oil pollution on algae and aquatic ecosystems in the context of global change. This research is of great importance for predicting future environmental changes. This review offers a more comprehensive understanding of the interactions between microplastics/nanoplastics and algae, as well as their impact on the carbon cycle.

Constructed wetlands as neglected fixed source of microplastics and antibiotic resistance genes in natural water bodies?

The pollution status and the harm caused by microplastics and antibiotic resistance genes (ARGs) in aquatic ecosystems have been a growing concern. The presence of microplastics could accelerate the transfer and spread of ARGs. Before sewage reaches natural water bodies, microplastics and ARGs need to be eliminated through specific processes. Constructed wetlands are currently an effective and environmentally friendly wastewater treatment process. Research has shown significant effectiveness in removing microplastics and ARGs. Microplastics and ARGs can be removed through processes such as adsorption, capture, adhesion, and biodegradation. However, long-term continuous operation could lead to constructed wetlands becoming significant reservoirs of microplastics and ARGs. Inflow loads and seasonal variations in constructed wetlands may result in the reintroduction of persistent microplastics and ARGs into the receiving water body, establishing the constructed wetland as a continuous source of these pollutants in the receiving water body. The key to the widespread application of constructed wetlands lies in solving this challenging problem. Therefore, although constructed wetlands serve as a green strategy for removing microplastics and ARGs, there are still many gaps in our knowledge. Based on the current accumulation of microplastics and ARGs in constructed wetlands, this paper summarizes the removal of microplastics and ARGs in existing constructed wetlands and explores the interaction between them. Additionally, it proposes suggestions for optimizing the process and improving the reliability of monitoring microplastics and ARGs in sewage.

Zn-doped MnO2 nanocoating with enhanced catalase-mimetic activity and cytocompatibility protects pre-osteoblasts against H2O2-induced oxidative stress.

Therapeutic application in prevention and treatment of bone diseases, particularly osteoporosis, has recently started to emerge for manganese dioxide (MnO2) nanoparticles and nanocoatings whereby their antioxidant catalase-mimetic property can be exploited to control oxidative stress by reducing the amount of H2O2. Doping is an efficient method to enhance the catalase-mimetic activity of MnO2, which can potentially ameliorate osteogenesis under oxidative stress. Herein, Zn2+ doped MnO2 (Zn-MnO2) nanocoating was fabricated on orthopedic titanium implant by a facile UV-photolysis reaction. The Zn-MnO2 nanocoating showed better cytocompatibility than the MnO2 nanocoating, as indicated by enhanced cell proliferation, differentiation and mineralization of MC3T3-E1 pre-osteoblasts. This was probably due to the increased surface hydrophilicity as well as the combination effect of released Zn2+ and Mn2+ from the Zn-MnO2 nanocoating. Importantly, the Zn-MnO2 nanocoating with enhanced catalase-like activity exerted greater effects to suppress the intracellular oxidation products generation and prevent the depletion of dismutase superoxide levels under H2O2-induced oxidative stress, which in turn protected MC3T3-E1 pre-osteoblast functions. Overall, surface modification of titanium implants with the Zn-MnO2 nanocoating could be utilized to ameliorate oxidative stress-inhibited osteogenesis.

Boron-incorporated micro/nano-topographical calcium silicate coating dictates osteo/angio-genesis and inflammatory response toward enhanced osseointegration.

Orthopedic implant coatings with optimal surface features to achieve favorable osteo/angio-genesis and inflammatory response would be of great importance. However, to date, few coatings are capable of fully satisfying these requirements. In this work, to take advantage of the structural complexity of micro/nano-topography and benefits of biological trace elements, two types of boron-containing nanostructures (nanoflakes and nanolamellars) were introduced onto plasma-sprayed calcium silicate (F-BCS and L-BCS) coatings via hydrothermal treatment. The C-CS coating using deionized water as hydrothermal medium served as control. Boron-incorporated CS coating stimulated osteoblastic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs). Specifically, the combination of ß1 integrin-vinculin-mediated cell spreading and activation of bone morphogenetic protein signaling pathway acted synergistically to cause significant upregulation of runt-related transcription factor 2 (RUNX2) protein and Runx2 gene expression in BMSCs on the F-BCS coating surface, which induced the transcription of downstream osteogenic differentiation marker genes. F-BCS coating allowed specific boron ion release, which favored angiogenesis as evidenced by the enhanced migration and tube formation of human umbilical vein endothelial cells in the coating extract. Boron-incorporated coatings significantly suppressed the expression of toll-like receptor adaptor genes in RAW264.7 macrophages and subsequently the degradation of nuclear factor-κB inhibitor α, accompanied by the inactivation of the downstream pro-inflammatory genes. In vivo experiments confirmed that F-BCS-coated Ti implant possessed enhanced osseointegration compared with L-BCS- and C-CS-coated implants. These data highlighted the synergistic effect of specific nanotopography and boron release from orthopedic implant coating on improvement of osseointegration.

[Evaluation of the effectiveness of treatment of 16 patients with tongue microcystic lymphatic malformation using bipolar coagulation forceps combined with 1% lauromacrogol foam sclerotherapy].

PURPOSE: To investigate the clinical efficacy and safety of bipolar coagulation forceps combined with 1% lauromacrogol foam sclerotherapy in the treatment of microcystic lymphatic malformation of tongue. METHODS: The clinical data of 16 patients with microcystic lymphatic malformation of the tongue admitted to the Department of Stomatology of Foshan First People's Hospital from April 2017 to April 2020 were retrospectively analyzed, including 7 males and 9 females, aging from 15 months to 21 years. The average age of patients was 8.32±1.21 years. The efficacy and complications of the patients were evaluated. RESULTS: All 16 patients were followed up for 6 to 12 months. The clinical response was evaluated as eleven patients (68.75%) for grade Ⅳ, three (18.75%) for grade Ⅲ, two (12.5%) for grade Ⅱ, and the effective rate was 87.5%. Necrosis of tongue mucosa and muscle occurred in 1 patient. CONCLUSIONS: Bipolar coagulation forceps combined with 1% lauromacrogol foam sclerotherapy is a minimally invasive, safe and effective treatment for tongue microcystic lymphatic malformation.

Hygiene practices among young adolescents aged 12-15 years in low- and middle-income countries: a population-based study.

BACKGROUND: Poor personal hygiene increases disease risk, however, the prevalence of hygiene practices among adolescents is poorly described in low- and middle-income countries (LMICs). We aimed to assess the hygiene practices among young adolescents in LMICs using data from the Global School-based Student Health Surveys (GSHS). METHODS: This population-based study analysed the GSHS data for adolescents aged 12-15 years from 75 LMICs. Data were collected between 2003 and 2015 using standardised, anonymous, self-reported questionnaires. This report focuses on hygiene related behaviours such as tooth brushing, washing hands after using the toilet, washing hands before eating and washing hands with soap. The weighted prevalence and 95% confidential intervals (CIs) for the hygiene practices, and overall and regional estimates were calculated with random-effects meta-analysis. RESULTS: A total of 181 848 young adolescents from 75 LIMCs with available hygiene data were analysed. The overall prevalence for never washing hands were 7.4% (95% confidence interval (CI) = 4.4-10.3) for before eating, 5.9% (95% CI = 3.8-7.9) for after using the toilet, and 9.0% (95% CI = 6.2-11.8) for with soap. For tooth brushing, the overall prevalence estimates were 8.6% (95% CI = 5.5-11.7) for never brushing teeth, 80.9% (95% CI = 74.7-87.1) for 1-3 times per day, and 9.7% (95% CI = 5.8-13.6) for >3 times per day. However, the prevalence of different hygiene practices varied significantly among countries and regions (all P values <0.001). Poor hygiene status, with a prevalence >20%, was still observed in several LMICs (eg, 12 countries for never washing hands and 4 for never brushing teeth). CONCLUSIONS: The hygiene practices of young adolescents aged 12-15 years were generally frequent, but poor status was still observed in several LMICs. These findings emphases the need for hygiene and health education targeting young adolescents in LMICs.

Key process parameters for deep eutectic solvents pretreatment of lignocellulosic biomass materials: A review.

Deep eutectic solvent (DES) has been considered as a novel green solvent for lignocellulosic biomass pretreatment. The efficiency of DES pretreatment is affected by the synergy of various process parameters. The study of effect of DES physicochemical properties and pretreatment reaction conditions on the mechanism of lignocellulose biomass fractionation was of great significance for the development of biomass conversion. Form the view of process control, this review summarized recent advances in DES pretreatment, analyzed the challenges, and prospected the future development of this research field.