Exploring the role of ITGB6: fibrosis, cancer, and other diseases.

Integrin ß6 (ITGB6), a member of the integrin family of proteins, is only present in epithelial tissues and frequently associates with integrin subunit αv to form transmembrane heterodimers named integrin αvß6. Importantly, ITGB6 determines αvß6 expression and availability. In addition to being engaged in organ fibrosis, ITGB6 is also directly linked to the emergence of cancer, periodontitis, and several potential genetic diseases. Therefore, it is of great significance to study the molecular-biological mechanism of ITGB6, which could provide novel insights for future clinical diagnosis and therapy. This review introduces the structure, distribution, and biological function of ITGB6. This review also expounds on ITGB6-related diseases, detailing the known biological effects of ITGB6.

Periodontal disease and Helicobacter pylori infection in oral cavity: a meta-analysis of 2727 participants mainly based on Asian studies.

OBJECTIVES: To assess the association between periodontal disease and Helicobacter pylori (H. pylori) infection in oral cavity. MATERIALS AND METHODS: We searched PubMed, Embase, Web of Science, Cochrane library, Gray literature, and clinicaltrials.gov for eligible studies up to September 25, 2019. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated. The random-effects model was used with the software STATA 13.0. The Newcastle-Ottawa-Scale was used for quality evaluation. RESULTS: Twelve observational studies (eight from Asia, one from Europe, and three from the South America) involving 2727 participants were included in the meta-analysis. The overall pooled results showed that H. pylori infection in oral cavity was associated with periodontal disease (OR 2.53, 95% CI 1.86-3.44, P < 0.05). No significant heterogeneity among the articles was observed (I2 = 44.3%, P < 0.05). The sensitivity analysis indicated that the result of our meta-analysis was generally stable. The Begg test and the Egger test both showed no publication bias was observed (P = 0.45 and P = 0.18 respectively). CONCLUSIONS: Based on current available evidence, it seemed there was a correlation between oral H. pylori infection and the occurrence of periodontal disease. However, since most of the data comes from Asia, more large-scale investigations with high quality from all over the world are needed to confirm the association. CLINICAL RELEVANCE: H. pylori infection in oral may have a positive association with the prevalence of periodontal disease mainly in Asian populations.

Distinct gene expression characteristics in epithelial cell-Porphyromonas gingivalis interactions by integrating transcriptome analyses.

Porphyromonas gingivalis is a pivotal periodontal pathogen, and the epithelial cells serve as the first physical barrier to defend the host from bacterial attack. Within this host-bacteria interaction, P. gingivalis can modify the host immune reaction and adjust the gene expression, which is associated with periodontitis pathogenesis and developing strategies. Herein, a meta-analysis was made to get the differential gene expression profiles in epithelial cells with or without P. gingivalis infection. The network-based meta-analysis program for gene expression profiling was used. Both the gene ontology analysis and the pathway enrichment analysis of the differentially expressed genes were conducted. Our results determined that 290 genes were consistently up-regulated in P. gingivalis infected epithelial cells. 229 gene ontology biological process terms of up-regulated genes were discovered, including "negative regulation of apoptotic process" and "positive regulation of cell proliferation/migration/angiogenesis". In addition to the well-known inflammatory signaling pathways, the pathway associated with a transcriptional misregulation in cancer has also been increased. Our findings indicated that P. gingivalis benefited from the survival of epithelial cells, and got its success as a colonizer in oral epithelium. The results also suggested that infection of P. gingivalis might contribute to oral cancer through chronic inflammation. Negative regulation of the apoptotic process and transcriptional misregulation in cancer pathway are important contributors to the cellular physiology changes during infection development, which have particular relevance to the pathogenesis and progressions of periodontitis, even to the occurrence of oral cancer.

Novel Electrochemical Sensor Fabricated for Individual and Simultaneous Ultrasensitive Determination of Olaquindox and Carbadox Based on MWCNT-OH/CMK-8 Hybrid Nanocomposite Film.

A hybrid nanocomposite consisting of hydroxylated multi-walled carbon nanotubes (MWCNTs-OH) and cube mesoporous carbon (CMK-8) was applied in this study to construct an MWCNT-OH/CMK-8/gold electrode (GE) electrochemical sensor and simultaneously perform the electro-reduction of olaquindox (OLA) and carbadox (CBX). The respective peak currents of CBX and OLA on the modified electrode increased by 720- and 595-fold relative to the peak current of GE. The performances of the modified electrode were investigated with electrochemical impedance spectroscopy, cyclic voltammetry, and differential pulse voltammetry. Then, the modified electrodes were used for the individual and simultaneous determination of OLA and CBX. The fabricated sensor demonstrated a linear response at 0.2-500 nmol/L in optimum experimental conditions, and the detection limits were 104.1 and 62.9 pmol/L for the simultaneous determination of OLA and CBX, respectively. As for individual determination, wide linear relationships were obtained for the detected OLA with levels of 0.05-500 nmol/L with LOD of 20.7 pmol/L and the detected CBX with levels of 0.10-500 nmol/L with LOD of 50.2 pmol/L. The fabricated sensor was successfully used in the independent and simultaneous determination of OLA and CBX in spiked pork samples.

Congenital Loss of Permanent Teeth in a Patient With Congenital Insensitivity to Pain With Anhidrosis due to 2 Novel Mutations in the NTRK1 Gene.

Congenital insensitivity to pain with anhidrosis (CIPA), also known as hereditary sensory and autonomic neuropathy type IV, is an extremely rare autosomal recessive disorder. This study investigated the oral and craniofacial manifestations of a 7-year-old Chinese boy affected by CIPA and identified 2 novel mutations in the NTRK1 gene, and a new feature of the disorder was identified. The patient had typical features, including insensitivity to pain, anhidrosis, and mental retardation; recurrent fractures and osteoporosis also were noted. His oral and craniofacial manifestations included congenital blepharoptosis, a large number of missing teeth, serious tooth abrasion, severe soft tissue injuries, and dental caries. Radiographic examination showed congenital loss of the permanent tooth germs, thin and weak alveolar bone of the mandible, and a fracture of the right mandible. This study extends the spectrum of NTRK1 mutations observed in patients with a diagnosis of CIPA and is the first to propose that congenital loss of permanent teeth may occur in CIPA patients. Furthermore, it highlights the importance of including an oral and maxillofacial surgeon and a pediatric dentist on the multidisciplinary team.

Selective extraction and determination of fluoroquinolones in bovine milk samples with montmorillonite magnetic molecularly imprinted polymers and capillary electrophoresis.

A sensitive and selective method for separating fluoroquinolones (FQs) from bovine milk samples was successfully developed using montmorillonite magnetic molecularly imprinted polymers (MMMIPs) as adsorbents. MMMIPs were prepared using montmorillonite as carrier, fleroxacin (FLE) as template molecule, and Fe3O4 magnetite as magnetic component. MMMIPs possessed high adsorption capacity of 46.3 mg g(-1) for FLE. A rapid and convenient magnetic solid-phase extraction procedure coupled with capillary electrophoresis was established with MMMIPs as adsorbents for simultaneous and selective extraction of four FQs in bovine milk samples. Limits of detection ranged between 12.9 and 18.8 µg L(-1), and the RSDs were between 1.8% and 8.6%. The proposed method was successfully applied to spike bovine milk samples with recoveries of 92.7%-108.6%.

Performance and mechanism of microbial fuel cell coupled with anaerobic membrane bioreactor system for fouling control.

To remove membrane fouling, a bio-electrochemical system that can generate a micro-electric field and micro-current was constructed. After 11 days of operation, the trans-membrane pressure difference of membrane modules in the open- and closed-circuit groups increased by 35.8 kPa and 6.2 kPa, respectively. The concentrations of total polysaccharide and protein in the open-circuit group were 1.8 and 1.1 times higher than those in the closed-circuit group, respectively. In addition, X-ray photoelectron spectroscopy and thermogravimetric analysis showed that inorganic crystals such as calcium carbonate were present on the membrane surface, and the concentration of calcium ion in the control group was 14.7 times that of the experimental group. High-throughput sequencing demonstrated that the enrichment of some electroactive bacteria and other microorganisms has a positive effect on the control of membrane fouling. Therefore, this system can effectively alleviate membrane fouling of a bioreactor, by targeting the membrane foulants.

Study on the performance and mechanism of bio-electrochemical system to mitigate membrane fouling in bioreactors.

To alleviate membrane fouling, a membrane of the membrane bioreactor was directly used as the anode of the bio-electrochemical system. On the 14th day, the control group had blocked, while the experimental group with a current of 0.44 mA, the increase in ΔTMP was only 2.2 kPa. The polysaccharide and protein concentrations in the open-circuit group were 4.2 and 2.9 times higher than those in the closed-circuit group, respectively. Three-dimensional fluorescence spectroscopy and gas chromatography mass spectrometry showed that most of the deposition in the control group contained high-molecular-weight compounds, especially long-chain ester derivatives, phenols, and complex hydrocarbons, whereas the experimental group was the opposite. Therefore, current (electrons) can change the composition of the cake layer. High-throughput sequencing indicated that a significantly higher abundance of electroactive microorganisms on the experimental than control group. Two-dimensional correlation spectroscopy showed that electrons promote the degradation of polysaccharides, thereby alleviating membrane fouling.

Porphyromonas gingivalis infection promotes mitochondrial dysfunction through Drp1-dependent mitochondrial fission in endothelial cells.

Porphyromonas gingivalis (P. gingivalis), a key pathogen in periodontitis, has been shown to accelerate the progression of atherosclerosis (AS). However, the definite mechanisms remain elusive. Emerging evidence supports an association between mitochondrial dysfunction and AS. In our study, the impact of P. gingivalis on mitochondrial dysfunction and the potential mechanism were investigated. The mitochondrial morphology of EA.hy926 cells infected with P. gingivalis was assessed by transmission electron microscopy, mitochondrial staining, and quantitative analysis of the mitochondrial network. Fluorescence staining and flow cytometry analysis were performed to determine mitochondrial reactive oxygen species (mtROS) and mitochondrial membrane potential (MMP) levels. Cellular ATP production was examined by a luminescence assay kit. The expression of key fusion and fission proteins was evaluated by western blot and immunofluorescence. Mdivi-1, a specific Drp1 inhibitor, was used to elucidate the role of Drp1 in mitochondrial dysfunction. Our findings showed that P. gingivalis infection induced mitochondrial fragmentation, increased the mtROS levels, and decreased the MMP and ATP concentration in vascular endothelial cells. We observed upregulation of Drp1 (Ser616) phosphorylation and translocation of Drp1 to mitochondria. Mdivi-1 blocked the mitochondrial fragmentation and dysfunction induced by P. gingivalis. Collectively, these results revealed that P. gingivalis infection promoted mitochondrial fragmentation and dysfunction, which was dependent on Drp1. Mitochondrial dysfunction may represent the mechanism by which P. gingivalis exacerbates atherosclerotic lesions.

Effects of water improvement and defluoridation on fluorosis-endemic areas in China: A meta-analysis.

This meta-analysis systematically evaluated the effects of water improvement and defluoridation on fluorosis-endemic areas in North and South China. The study used PubMed, Embase, China National Knowledge Infrastructure, and Wanfang to retrieve relevant research studies published between January 2000 and October 2019. The data included water fluoride levels, dental fluorosis prevalence in children 8-15 years of age, urinary fluoride levels in children and adults, and skeletal fluorosis prevalence in adults. Fixed-effects and random-effects models were used in the meta-analysis. A total of 17 research articles met the inclusion criteria and had an average water improvement period of 15.8 years. With water improvement, water fluoride levels decreased from 2.72 mg/L to 0.54 mg/L (95% confidence intervals: -2.75, -1.58), which was below the standard for drinking water (1.5 mg/L). Additionally, after water improvement, the prevalence of dental fluorosis decreased from 54.5% to 36.2% (95% confidence intervals: 0.12, 0.31) in children, and the prevalence of skeletal fluorosis decreased from 13.7% to 4.2% (95% confidence intervals: 0.16, 0.40) in adults. Urinary fluoride levels decreased from 3.06 mg/L to 1.70 mg/L (OR = -2.03, 95% confidence intervals: -2.77, -1.30) in children and from 2.29 mg/L to 1.72 mg/L (OR = -0.57, 95% confidence intervals: 0.65, -0.49) in adults. The results showed that the prevalence of dental fluorosis and skeletal fluorosis and urinary fluoride levels were significantly reduced by water improvement. This study findings revealed that the effects of water improvement and defluoridation were greater in South China than in North China, and it is obviously related to the time of water improvement and reducing fluoride.

Salivary cortisol concentrations in police officers - a cross-sectional study in Beijing, China.

OBJECTIVE: We investigated the relationship between salivary cortisol level and the prevalence of depression 585 police officers working at the Police Departments of Beijing. METHOD: Cross-sectional data were obtained from 585 Chinese police officers recruited from Beijing, China. Salivary cortisol was assayed using the chemiluminescence immunoassay. A multiple logistic regression analysis adjusted for potential confounders was used to assess independent associations between salivary cortisol level and depression. RESULTS: The median age of the included was 38 years (IQR, 29-45), 20.9% were female (n = 122). Finally, 15.6% (91/585; 95% CI: 12.6-18.5%) were considered to have depression. The median salivary cortisol level was significantly higher in police with depression than those police without depression [14.5(IQR, 11.9-15.9) nmol/l vs. 11.8(IQR, 9.4-14.2) nmol/l; P < 0.001]. The depression distribution across the salivary cortisol quartiles ranged between 5.4% (first quartile) and 26.9% (fourth quartile), P for trend <0.001. In multivariate models comparing the second (Q2), third and fourth quartiles against the first quartile of the salivary cortisol, cortisol in Q3 and Q4 were associated with depression, and increased prevalence of depression by 148% (OR: 2.48; 95% CI: 1.55-3.86) and 277% (3.77; 2.12-5.36). Based on ROC curves, the optimal cutoff value of salivary cortisol level to diagnose the depression was 13.8 nmol/l, which yielded the highest sensitivity and specificity [63.8% and 71.7%, respectively; area under the curve (AUC) = 0.695, 95% CI: 0.639-0.751; P < 0.0001]. CONCLUSIONS: The data showed that elevated levels of salivary cortisol were associated with increased prevalence of depression.

The Additional Effect of Autologous Platelet Concentrates to Coronally Advanced Flap in the Treatment of Gingival Recessions: A Systematic Review and Meta-Analysis.

BACKGROUND: To improve the efficacy of regenerative treatment for gingival recessions, the autologous platelet concentrates (APCs) combined with coronally advanced flap (CAF) have been investigated. However, few studies systematically assess the complementary effect of APCs in periodontal regeneration. The present study aims to evaluate the additional effect of different types of APCs to CAF in the treatment of gingival recessions. METHODS: Electronic databases (EMBASE, MEDLINE, and Cochrane Central Register of Controlled Trails) and relevant journals were searched until May 15, 2019. Only randomized controlled trials (RCTs) in English were included. Outcome variables include root coverage (RC), recession depth (RD), clinical attachment level (CAL), keratinized tissue width (KTW), and gingival thickness (GT). Data were analyzed with Revman5.3. The estimate of effect sizes was expressed as the mean differences and the 95% confidence interval. RESULTS: 8 RCTs involving 170 patients (328 sites) were included. Our meta-analysis indicated RC, RD, CAL, KTW, and GT were better improved in the CAF plus APCs groups than the CAF alone. The subgroup analyses revealed that platelet-rich fibrin (PRF) brought significant improvement in RC, RD, CAL, and GT. Concentrated growth factors (CGF) lead clinic beneficial in CAL, KTW, and GT. No significant effect of platelet-rich plasma (PRP) could be found in any clinical parameters when combined with CAF. CONCLUSIONS: PRF could exert additional effect to CAF; the preferred treatment for gingival recessions was considered. Based on the limited studies, it seemed that PRP failed to show any additional effect and it was not suggested for gingival recessions. Given the limited research and high risk of bias, it is still needed to confirm the additional effect of CGF by more high-quality studies.

Effect of platelet-rich fibrin on alveolar ridge preservation: A systematic review.

BACKGROUND: Platelet-rich fibrin (PRF) is the second generation of platelet concentrates and is used in many areas of dentistry. However, whether PRF is effective for alveolar ridge preservation remains controversial. The authors conducted research to evaluate the potential of PRF to preserve the alveolar ridge. METHODS: A comprehensive literature search was conducted in MEDLINE, Cochrane Central Register of Controlled Trials, and Embase. Only randomized controlled trials were included. A systematic review was made for postoperative pain, soft-tissue healing, bone density, horizontal and vertical ridge dimension changes, and histologic analysis. The meta-analysis was performed on the alveolar osteitis, mesial and distal bone height changes, and bone fill with Review Manager Version 5.3 software. RESULTS: Among the 588 eligible articles found in the initial search, 7 published studies from 2012 through 2019 were included. The authors' qualitative analysis showed that PRF may play a positive role in reducing postoperative pain and ridge dimension changes after tooth extraction. Among the 7 articles, only 2 trials assessed the effect of PRF on the alveolar osteitis, mesial and distal bone height changes, and bone fill. Results of our meta-analysis showed that smaller mesial bone height changes (standard mean difference, -1.07; 95% confidence interval, -1.92 to 0.22) and a greater percentage of bone fill (standard mean difference, 0.82; 95% confidence interval, 0.32 to 1.33) were observed in the PRF group. CONCLUSIONS: Given the potential value of PRF, consideration should be given to PRF after tooth extraction. However, more high-quality trials are necessary to evaluate the exact role of PRF. PRACTICAL IMPLICATIONS: Based on the authors' results, the usage of PRF was suggested in alveolar ridge preservation.

The expression of macrophage migration inhibitory factor and intercellular adhesion molecule-1 in rats with periodontitis and atherosclerosis.

OBJECTIVE: The aim of this study was to explore the role of macrophage migration inhibitory factor (MIF) and intercellular adhesion molecule (ICAM)-1 in rats with periodontitis and atherosclerosis. METHODS: Twenty-four male Sprague-Dawley rats were randomly divided into 4 groups: control group (C), periodontitis group (P), atherosclerosis group (AS), and periodontitis plus atherosclerosis group (P + AS). The levels of MIF and ICAM-1 in serum were detected by an enzyme-linked immunosorbent assay (ELISA). The protein expression of MIF and ICAM-1 in the carotid artery tissues was examined by immunohistochemical staining. RESULTS: The results of the ELISA showed that the serum level of MIF in the P + AS group (59.40 ±â€¯3.92 ng/mL) was significantly higher than that in the C group (42.93 ±â€¯2.63 ng/mL), the P group (45.57 ±â€¯2.59 ng/mL) and the AS group (50.88 ±â€¯4.20 ng/mL) (P < 0.05). Similarly, the ICAM-1 level in the P + AS group (6.77 ±â€¯1.47 ng/mL) was much higher than that in the C (1.33 ±â€¯0.25 ng/mL), P (3.99 ±â€¯0.44 ng/mL) and AS groups (4.19 ±â€¯0.89 ng/mL) (P < 0.05). Furthermore, the expression of MIF, as assessed by immunohistochemical staining, was significantly higher in the artery tissues of the P + AS group than in the tissues of the other three groups (P < 0.05). However, there was no significant difference in the protein level of ICAM-1 between the P + AS and AS groups, where its expression was much stronger than in the C and the P groups. CONCLUSION: Our results indicate that there is a close association between periodontitis and atherosclerosis. MIF and ICAM-1 may play a role in the development of atherosclerosis and periodontitis.

Porphyromonas gingivalis-Induced MIF Regulates Intercellular Adhesion Molecule-1 Expression in EA.hy926 Cells and Monocyte-Endothelial Cell Adhesion Through the Receptors CD74 and CXCR4.

Porphyromonas gingivalis (P. gingivalis) is an important pathogen that contributes to periodontal disease and causes infections that promote the progression of atherosclerosis. Our previous studies showed that macrophage migration inhibitory factor (MIF) facilitates monocyte adhesion to endothelial cells by regulating the expression of intercellular adhesion molecule-1 (ICAM-1) in P. gingivalis-infected endothelial cells. However, the detailed pathological role of MIF has yet to be elucidated in this context. To explore the functional receptor(s) of MIF that underlie its participation in the pathogenesis of atherosclerosis, we investigated the expression of the chemokine receptors CD74 and CXCR4 in endothelial cells, both of which were shown to be involved in the adhesion of monocytes to endothelial cells pretreated with P. gingivalis. Furthermore, the formation of a MIF, CD74, and CXCR4 ligand-receptor complex was revealed by our immunofluorescence staining and coimmunoprecipitation results. By interacting with the CD74/CXCR4 receptor complex, MIF may act as a crucial regulator of monocyte-endothelial cell adhesion and promote the atherosclerotic plaque formation induced by P. gingivalis.

Selective recognization of dicyandiamide in bovine milk by mesoporous silica SBA-15 supported dicyandiamide imprinted polymer based on surface molecularly imprinting technique.

In this work, a highly selective molecular imprinted polymer (SBA-15@MIP) was prepared using dicyandiamide (DCD) as the template molecule and mesoporous silica SBA-15 as the carrier. The resulting polymers were characterized by scanning electron microscopy and FT-IR spectra. A highly ordered mesoporous nanostructure was observed for the fabricated polymer. The performance tests showed that the adsorption capacity of SBA-15@MIP for DCD was higher than those of non-imprinted mesoporous silica polymer (SBA-15@NIP) and SBA-15. SBA-15@MIP was successfully used as the solid-phase extraction (SPE) sorbent coupled with HPLC for trace DCD determination in bovine milk and milk powder. Under selected conditions, a favorable linearity between 1µg/mL and 100µg/mL was obtained and the detection limit was estimated to be 0.08µg/mL (bovine milk) and 0.10µg/mL (milk powder). These results indicated that SBA-15@MIP could be used as an efficient SPE adsorbent for enrichment of DCD in bovine milk and milk powder.

Molecularly imprinted electrochemical sensor based on Au nanoparticles in carboxylated multi-walled carbon nanotubes for sensitive determination of olaquindox in food and feedstuffs.

A novel molecularly imprinted electrochemical sensor (MIECS) was proposed to determine olaquindox (OLA) using gold nanoparticles in molecularly imprinted polymer (AuNPs@MIP) and carboxylated multi-walled carbon nanotubes (cMWCNTs). Glassy carbon electrode (GCE) was modified with cMWCNTs (cMWCNTs/GCE), and AuNPs/cMWCNT/GCE was obtained by electrodeposition on cMWCNTs/GCE using chronoamperometry in HAuCl4. Then, the obtained MIP/AuNPs/cMWCNTs/GCE was electropolymerized using OLA as template and o-PD as monomer to determine OLA. Important experimental parameters, namely, scan cycles, mole ratio of template molecules to functional monomers, pH value, and incubation time were optimized. The novel MIP sensor can offer a 2.7nM of detection limit for OLA. In addition, a series of food and feedstuffs were analyzed to demonstrate the feasibility of MIECS.

Risk Factors for Intraoperative Proximal Femoral Fracture During Primary Cementless THA.

Intraoperative proximal femoral fracture, one of the most common complications of total hip arthroplasty (THA), occurs more often in cementless procedures and can affect rehabilitation, hospitalization time, and cost of treatment. The goal of this study was to identify risk factors for intraoperative proximal femoral fracture in THA to identify high-risk groups preoperatively and minimize the incidence of this complication. This nested case-control study included 904 primary cementless THA procedures (769 patients) performed between January 2009 and July 2015. Of this group, 24 fractures occurred, accounting for 2.65% of cases. Predisposing factors for intraoperative proximal femoral fracture from the medical records included patient sex, diagnosis of osteoarthritis, operated on hip (left or right), type of implant, alcohol consumption, operative approach, age, and body mass index. The Noble classification, Dorr classification, and Metaphyseal-Diaphyseal Index score measured by picture archiving and communication systems were used to evaluate the anatomy and morphologic features of the proximal femur. A multivariate analysis was performed to evaluate potential risk factors for fracture during THA, including anterolateral (modified Hardinge) approach, use of the Corail (DePuy, Warsaw, Indiana) stem, Metaphyseal-Diaphyseal Index score, age, and sex. A Corail stem, the anterolateral approach, advanced age, and a low Metaphyseal-Diaphyseal Index score were associated with increased risk of fracture. All fractures were treated with cerclage wire techniques, and none has required revision to date. [Orthopedics. 2017; 40(2):e281-e287.].