RESUMO
Oleoyl-carboxymethy chitosan (OCMCS) nanoparticles based on chitosan with different molecular weights (50, 170 and 820 kDa) were prepared by self-assembled method. The nanoparticles had spherical shape, positive surface charges and the mean diameters were 157.4, 274.1 and 396.7 nm, respectively. FITC-labeled OCMCS nanoparticles were internalized via the intestinal mucosa and observed in liver, spleen, intestine and heart following oral deliverance to carps (Cyprinus carpio). Extracellular products (ECPs) of Aeromonas hydrophila as microbial antigen was efficiently loaded to form OCMCS-ECPs nanoparticles and shown to be sustained release in PBS. Significantly higher (P < 0.05) antigen-specific antibodies were detected in serum after orally immunized with OCMCS-ECPs nanoparticles than that immunized with ECPs alone and non-immunized in control group in carps. These results implied that amphiphilic modified chitosan nanoparticles had great potential to be applied as carriers for the oral administration of protein drugs.
Assuntos
Quitosana/análogos & derivados , Nanopartículas/química , Nanotecnologia/métodos , Administração Oral , Aeromonas/metabolismo , Animais , Anti-Infecciosos/farmacologia , Antígenos/química , Materiais Biocompatíveis/química , Carpas , Quitosana/química , Portadores de Fármacos/química , Sistemas de Liberação de Medicamentos , Luz , Teste de Materiais , Microscopia Eletrônica de Transmissão/métodos , Peso Molecular , Solubilidade , Estresse Mecânico , Água/químicaRESUMO
Two types of poly-lactic-co-glycolic acid (PLGA) based nanoparticles were prepared by surface modification (C-NPs) and homogeneous modification (G-NPs) with chitosan. The naked PLGA NPs were served as control. These nanoparticles all showed regularly spherical shape with mean diameters as 191.3 ± 3.6 nm, 211.9 ± 13.2 nm and 187.5 ± 17.6 nm for PLGA NPs, C-NPs and G-NPs, respectively. Their zeta potentials were -22.4 ± 1.31 mV, -8.7 ± 0.45 mV, -3.1 ± 0.12 mV, respectively. Both C-NPs (15.3% PLGA remained after 2 weeks) and G-NPs (3.7% PLGA remained after 2 weeks) had higher hydrolysis rate than PLGA NPs (18.4% PLGA remained after 6 weeks), with G-NPs showing the highest rate in hydrolysis due to the incorporation of chitosan and its self-assembled structure. Self-assembling properties and controllable biodegradability of G-NPs indicated that it could be a promising drug delivery carrier for tumor drug delivery.