Nanoencapsulation of R-phycoerytrin extracted from Solieria filiformis improves protein stability and enables its biological application as a fluorescent dye.

We aimed to encapsulate R-PE to improve its stability for use as a fluorescent probe for cancer cells. Purified R-PE from the algae Solieria filiformis was encapsulated in polymeric nanoparticles using PCL. Nanoparticles were characterised and R-PE release was evaluated. Also, cellular uptake using breast and prostate cancer cells were performed. Nanoparticles presented nanometric particle size (198.8 ± 0.06 nm) with low polydispersity (0.13 ± 0.022), negative zeta potential (-18.7 ± 1.10 mV), and 50.0 ± 7.3% encapsulation. FTIR revealed that R-PE is molecularly dispersed in PCL. DSC peak at 307 °C indicates the presence of R-PE in the nanoparticle. Also, in vitro, it was demonstrated low release for nanoparticles and degradation for the free R-PE. Finally, cellular uptake demonstrated the potential of R-PE/PCL nanoparticles for cancer cell detection. Nanoparticles loaded with R-PE can overcome instability and allow application as a fluorescent probe for cancer cells.

Mechanism of Action of TiF4 on Dental Enamel Surface: SEM/EDX, KOH-Soluble F, and X-Ray Diffraction Analysis.

This in vitro study aimed to evaluate the action of TiF4 on sound and carious bovine and human enamel. Sound (S) and pre-demineralised (DE) bovine and human (primary and permanent) enamel samples were treated with TiF4 (pH 1.0) or NaF varnishes (pH 5.0), containing 0.95, 1.95, or 2.45% F for 12 h. The enamel surfaces were analysed using SEM-EDX (scanning electron microscopy/energy-dispersive X-ray spectroscopy) (n = 10, 5 S and 5 DE) and KOH-soluble fluoride was quantified (n = 20, 10 S and 10 DE). Hydroxyapatite powder produced by precipitation method was treated with the corresponding fluoride solutions for 1 min (n = 2). The formed compounds were detected using X-ray diffraction (XRD). All TiF4 varnishes produced a coating layer rich in Ti and F on all types of enamel surface, with micro-cracks in its extension. TiF4 (1.95 and 2.45% F) provided higher fluoride deposition than NaF, especially for bovine enamel (p < 0.0001). It also induced a higher fluoride deposition on DE samples compared to S samples (p < 0.0001), except for primary enamel. The Ti content was higher for bovine and human primary enamel than human permanent enamel, with some differences between S and DE. The XRD analysis showed that TiF4 induced the formation of new compounds such as CaF2, TiO2, and Ti(HPO4)2·H2O. In conclusion, TiF4 (>0.95% F) interacts better, when compared to NaF, with bovine and human primary enamel than with human permanent enamel. TiF4 provoked higher F deposition compared to NaF. Carious enamel showed higher F uptake than sound enamel by TiF4 application, while Ti uptake was dependent on the enamel condition and origin.

Absenteísmo e suas causas no contexto da saúde dos trabalhadores em oncologia: revisão integrativa / Absenteeism and it's causes in the context of oncology workers health: integrative review / Ausentismo y sus causas en el contexto de la salud de los trabajadores en oncología: revisión integradora

O ritmo elevado de trabalho, somado às demandas físicas e psicológicas, levam ao estresse nos contextos pessoal e laboral, o que faz com que as pessoas se afastem de seus ambientes de trabalho como um dos motivos apontados para a incapacidade para o trabalho. Essa realidade tem sido amplamente observada no ambiente hospitalar, possivelmente associada a problemas relacionados à fadiga da compaixão, geralmente em serviços de oncologia. Dessa forma, a motivação deste estudo foi compreender os motivos do absenteísmo em oncologia, e se esse episódio ocorre devido ao processo de trabalho. Objetivo:Investigar as causas do absenteísmo entre profissionais expostos a riscos ambientais e biopsicossociais em hospitais oncológicos. Metodologia:Trata-se de uma revisaointegrativa sobre o tema do absenteísmo, o que indica novos rumos para futuras investigações. Foi realizada uma revisão da literatura com base em três pilares: 1) O processo de trabalho multidisciplinar em oncologia e o risco de adoecimento; 2) O absentismo dos profissionais de saúde em oncologia; 3) O problema da pandemia de COVID-19 para os trabalhadores da saúde. Posteriormente, foram escolhidos os descritores e a partir deles foram realizadas buscas nas bases de dados eletrônicas PUBMED, LILACS e SCOPUS. Resultados:Obteve-se um resultado de dez estudos. Constatou-se que os principais transtornos, que levam à incapacidade para o trabalho e, por sua vez, ao absenteísmo, foram de origem psíquica (depressão e Síndrome de Burnout) e de origem musculoesquelética. Conclusões:A dupla jornada de trabalho foi citada como fator facilitador para o aparecimento desses transtornos, onde tais cenários não incapacitam o trabalhador para o desenvolvimento de suas atividades, que podem ser temporárias ou permanentes (AU).

The high pace of work, added to the physical and psychological demands, lead to stress in personal and work contexts, which causes people to withdraw from their work environments as one of the reasons mentioned for incapacitation for work. This reality hasbeen widely observed in the hospital setting, possibly associated with problems related to compassion fatigue, usually in oncology services. The motivation of this study was to understand the reasons for absenteeism in oncology, and if this episode occursdue to the work process. Objective:Investigating the causes of absenteeism among professionals exposed to environmental and biopsychosocial risks in cancer hospitals. Methodology:This is a integrative review on the theme of absenteeism, which indicates new directions for future investigations. A literature review was carried out based on three pillars: 1) The multidisciplinary work process in oncology and the risk of illness; 2) The absenteeism of health professionals in oncology; 3) The problem of the COVID-19 pandemic for health workers. Subsequently, the descriptors were chosen and based on them, searches were carried out in the electronic databases PUBMED, LILACS and SCOPUS. Results:A result of ten studies was obtained. It was found that the main disorders, which lead to incapacity for work and, in turn, absenteeism, were of psychic origin (depression and Burnout Syndrome) and of musculoskeletal origin. Conclusions: Texto das conclusões em inglêsThe double work shift was cited as a facilitating factorfor the appearance of these disorders, where such scenarios do not incapacitate the worker to develop their activities, which may be temporary or permanent (AU).

El alto ritmo de trabajo, sumado a las exigencias físicas y psicológicas, genera estrés en el contexto personal y laboral, lo que provoca que las personas se alejen de sus ambientes laborales como una de las razones esgrimidas para la incapacidad detrabajar. Esta realidad ha sido ampliamente observada en el ambiente hospitalario, posiblemente asociada a problemas relacionados con la fatiga por compasión, generalmente en los servicios de oncología. Así, la motivación de este estudio fue comprender las razones del ausentismo en oncología y si este episodio ocurre debido al proceso de trabajo. Objetivo:Investigar las causas del ausentismo entre profesionales expuestos a riesgos ambientales y biopsicosociales en hospitales oncológicos. Metodología:Se trata deuna revisión integradorasobre el tema del ausentismo, que indica nuevos rumbos para futuras investigaciones. Se realizó una revisión de la literatura basada en tres pilares: 1) El proceso de trabajo multidisciplinario en oncología y el riesgo de enfermedad; 2) El ausentismo de los profesionales de la salud en oncología; 3) El problema de la pandemia de COVID-19 para los trabajadores de la salud. Posteriormente se eligieron los descriptores y a partir de ellos se realizaron búsquedas en las bases de datos electrónicas PUBMED, LILACS y SCOPUS. Resultados:Se obtuvo un resultado de diez estudios. Se encontró que los principales trastornos que conducen a la incapacidad para trabajar y, a su vez, al ausentismo, fueron de origen psíquico (depresión y síndrome deBurnout) y de origen músculoesquelético. Conclusiones:La doble jornada laboral fue citada como un factor facilitador para la aparición de estos trastornos, dondedichos escenarios no incapacitan al trabajador para el desarrollo de sus actividades, las cuales pueden ser temporales o permanentes (AU).

Arsenicum albumas a treatment for pneumonia in snakes: three serial cases

two Python bivittatussnakes were received at Project Selva Viva, a zoo in Taubaté, Brazil, both presenting respiratory noises and oro-nasal discharge. A thoracic ultrasonographic examination was performed for evaluation on February 25th,2022, which diagnosed the presence of multiple vertical hyperechoic artifactsemerging from the pleural line, coalescing in some of the examined areas, and the presence a hypoechoic structure located in the subpleural region. Another serpent, from the Boa constrictor species, was apprehended and arrived at the same zoo without medical history, showing a low body score. During a thoracic ultrasonographic evaluation realized on April 1st,2022, B lines emerging from the pleural line were found. These vertical reverberating lines are a result of respiratory illness (SOLDATI et al., 2014).Methodology:The report was authorized by the owner of the zoo. All the patients received homeopathic therapy with 2 globules of Arsenicum album30 cH/ BID into the mouth, after being diagnosed with the respiratory condition by the ultrasonographic examination. The medication was chosen according to the similarity with the symptoms. The snakes had a runny nose and hissing breathing noise. Weekly ultrasound scans were performed on the Python bivittatus snakes to follow up on the respiratory condition. Results: On March 04th, it was observed that both snakes showed a reduction in respiratory noises and were more active. Ars 30 cH was maintained BID. On March 25th, both presented significant improvement in the ultrasound images, which showed only A lines, compatible with a healthy lung, and the treatment was suspended. Boa constrictor snake ́streatment started on April 1st. On April 8th, the ultrasonographic examination performed only presented A lines, which are characterized in ultrasound by parallel horizontal lines in the near field with the loss of image continuity in the far field, indicating improvement of the condition (LICHTENSTEIN et al., 2003). Conclusion:Given these results, the homeopathic treatment is an option to be considered for the treatment of respiratory symptoms in snakes, although the duration of the therapy varies based on the stage and chronicity of the disease.

Prevalence of oral lesions diagnosed at the ULBRA Canoas of Dental Diagnosis Service / Prevalência das lesões bucais observadas no Serviço de Diagnóstico Bucal da ULBRA Canoas

Objective: To evaluate the prevalence of oral lesions diagnosed at the ULBRA Canoas of Dental Diagnosis Service, from 2010 to 2016. Methods: This is a cross-sectional descriptive study based on the analysis of the biopsy files, filed in the Laboratory of Histology of ULBRA Canoas. A database was created with all the information collected in the biopsy record and the analysis of the data was performed by the STATA data processor. Results: The sample consisted of 405 patients, mean age was 45.7 years (18.6%); The majority of the patients were female (52.0%); and caucasian (81.2%). The majority of the lesions presented rosea staining (25.7%), defined limits and sessile base (32.6%), firm consistency (25.4%). The most frequent groups of oral lesions were: soft tissue neoplasms (20.5%), reactional lesions (17.5%), periapical pathologies (11.8%) and potentially malignant lesions (11.8%). Among the soft tissue neoplasms the most prevalent were fibroma (43.4%) and papiloma (31.3%). Conclusion: Thus, this study emphasizes the importance of knowledge by dentistry on the prevalence of oral lesions in the metropolitan region of Porto Alegre, such knowledge is essential for oral health care actions to be based on the epidemiological profile and frequency of lesions observed in this population.

Objetivos: Avaliar a prevalência de lesões bucais observadas no Serviço de Diagnóstico Bucal da ULBRA Canoas no período de 2010 a 2016. Métodos: estudo transversal descritivo a partir da análise das fichas de biópsia arquivadas no laboratório de Histologia da ULBRA Canoas. Foi criada uma base de dados com todas as informações coletadas na ficha de biópsia e a análise dos dados foi realizada pelo processador de dados STATA. Resultados: A amostra foi composta por 405 pacientes, média de idade foi de 45,7 anos (18,6%); a maioria dos pacientes era do sexo feminino (52,0%); e de raça branca (81,2%). O tipo de biópsia de maior prevalência foi a excisional/total (78,3%) A maioria das lesões apresentavam coloração rósea (25,7%), limites definidas e base séssil (32,6%), consistência firme (25,4%) e superfície lisa (46,4%), por fim, os grupos mais frequentemente encontrados de lesões bucais foram: neoplasias de tecido mole 83 (20,5%), lesões reacionais 71 (17,5%), patologias periapicais 48 (11,8%) e lesões potencialmente malignas 48 (11,8%). Dentre as neoplasias de tecido mole as mais prevalentes foram fibroma 36 (43,4%) e papiloma 26 (31,3%). Conclusão: Este estudo ressalta a importância do conhecimento pelos cirurgiões-dentistas sobre a prevalência de lesões bucais na região metropolitana de Porto Alegre, com intuito de promover ações de assistência à saúde bucal, que possam estar baseadas no perfil epidemiológico e na frequência das lesões observadas nesta população.

Educação em saúde com adolescentes: análise da aquisição de conhecimentos sobre temas de saúde / Health education with adolescents: analysis of knowledge acquisition on health topics / Educación en salud para adolescentes: análisis de la adquisición de conocimientos sobre temas de salud

Objetivo:O estudo tem por objetivo analisar a aquisição de conhecimentos sobre os temas: Saúde Bucal, Prevenção ao uso de Drogas e Sexualidade, junto a adolescentes matriculados na rede pública de ensino do Sul de Santa Catarina.Métodos:Pesquisa de campo, temporal, prospectiva, analítica, realizada em duas escolas públicas do sul catarinense. Amostra de adolescentes de ambos os sexos, idades entre 11 e 17 anos, variou conforme tema: 108 (saúde bucal), 105 (prevenção-drogas), 99 (sexualidade). Para cada ação foi construído e aplicado questionário (pré/pós-ação) com temas específicos. Pesquisa aprovada Comitê Ética nº 278.224/2013.Resultados:As ações, mesmo que pontuais, apresentaram resultados positivos quanto ao aumento de conhecimento dos adolescentes nas temáticas sobre prevenção de drogas e sexualidade, fato que não se configurou na temática saúde bucal.Conclusão:Os resultados estão relacionados a fatores que despertaram o interesse dos adolescentes como: métodos empregados que favoreceram o diálogo, troca de experiências e reflexão sobre as próprias práticas, relacionamentos com grupos de amigos/famílias.

Objective:The study aimed to analyze the acquisition of knowledge on three themes: Oral Health, Drug use Prevention and Sexuality. The research subjects were adolescents enrolled in public schools, in the south of Santa Catarina state.Methods:It is a field research, temporal, prospective, analytical, carried out in two public schools on the mentioned state. The sample size of adolescents, of both sexes, aged 11-17 years, varied according the theme: 108 (oral health), 105 (drugs prevention), 99 (sexuality). For every action, it was developed and implemented a questionnaire (pre/post-action) with specific topics. The research was approved by the Ethics Committee of Research.Results:The actions, although punctual, showed positive results in increasing the knowledge of adolescents on drug prevention and sexuality, which did not happen on the theme of oral health.Conclusion:The results are related to factors that aroused the interest of adolescents, for example, methods which promote dialogue, exchange of experiences, reflect on their own practices and relationships with groups of friends/family.

Objetivo:Analizar la adquisición de conocimientos sobre los temas Salud Bucal, Prevención al Uso de Drogas y Sexualidad, tratados con adolescentes matriculados en escuelas públicas de Santa Catarina.Métodos:Investigación de campo, temporal, prospectiva, analítica, realizada en dos escuelas públicas. Participaron adolescentes de ambos sexos, entre 11 y 17 años: 108 (salud oral); 105 (prevención contra las drogas); 99 (sexualidad). Para cada acción, se aplicó un cuestionario (pre/post acción) con temas específicos. Investigación aprobada en el Comité de Ética nº 278.224/2013.Resultados:Las acciones, aunque puntuales, mostraron resultados positivos cuanto al aumento de los conocimientos de los adolescentes sobre prevención de drogas y sexualidad, lo que no se ha constatado en la temática de salud bucal.Conclusión:Resultados relacionados con factores que despertaron el interés de los adolescentes como: métodos empleados que favorecieron el diálogo, intercambio de experiencias y reflexión sobre sus propias prácticas, las relaciones con los grupos de amigos/familiares.