RESUMO
The synthesis and antitumour and antibacterial activity of coumarin and chromone phosphorohydrazones have been reported. This study describes influence of phosphorohydrazones derivatives of coumarin and chromone on the polymerization and viscosity of fibrin. The fibrin polymerization assay was performed by the Shen and Lorand method and the clot viscosity was measured on the basis of Shen and Lorand and Marchi and coworkers methods. Among the eight compounds tested, one coumarin derivative and two chromone derivatives showed significant activity.
Assuntos
Cromonas/farmacologia , Cumarínicos/farmacologia , Fibrina/química , Fibrina/efeitos dos fármacos , Biopolímeros/química , Cromonas/química , Cumarínicos/química , Humanos , Técnicas In Vitro , Estrutura Molecular , Espectrofotometria Ultravioleta , Viscosidade/efeitos dos fármacosRESUMO
Microcrystalline chitosan (MCCh) characterise by many valuable features, including biocompatibility, antibacterial, fungicidal and high adhesive properties, high water retention value (WRV) factor, potent sorption, high bioactivity, biodegradability, ability to create membranes directly out of water suspensions. This biopolymer due to its biodegradability and safety in application no cytotoxic or immune interaction was assessed as a very effective haemostatic factor. MCCh with such properties form useful initial material for biological bandages; it can be used in surgery as growth factor carrier. Biodegradable mambranes are applied in surgery for guided tissue regeneration, what contributed to make the manufacturing technology of modified membranes out of microcrystalline chitosan. Peformed research was to show how certain angiogenic factors are released--platelet derived angiogenic factor (PDAF-AB) and transforming factor beta (TGF-beta) from doubled-layered alginate-chitosane membrane which was already used in dental surgery. Assessment of angiogenic factors release (PDGF-AB and TGF-beta) from polymer base may become helpful in choosing the right carrier for growth factors.
Assuntos
Indutores da Angiogênese/química , Bandagens , Quitosana/química , Portadores de Fármacos , Membranas Artificiais , Fator de Crescimento Derivado de Plaquetas/análise , Fator de Crescimento Transformador beta/análise , Materiais Biocompatíveis , Biodegradação Ambiental , Concentração de Íons de Hidrogênio , Fator de Crescimento Derivado de Plaquetas/química , Fator de Crescimento Transformador beta/químicaRESUMO
The research was aimed at determining the abundance and viability of biofilm formed on the surface of polylactide (PLA) during its biodegradation in different environments. It was also aimed at isolating biofilm forming bacteria, determining their hydrolytic activity and taxonomic status. The first step was to evaluate PLA biodegradability in lake water, compost and soil, using OxiTop Control. The next step was to assess the ability of isolated bacteria to form biofilm in the investigated environments and to evaluate the biofilm structure. The results indicate that PLA is sensitive to biodegradation in any environment, particularly in compost. During this process biofilm of high viability was observed on the surface of PLA. Based on the 16S rRNA gene sequence, the biofilm-forming bacteria were classified as the following species: Acidovorax sp. LW9, Chryseobacterium sp. LW2, Aeromonas veronii LW8, Arthrobacter aurescens LG2, Arthrobacter sp. LG12, A. aurescens LG9, Elizabethkingia meningoseptica LK3, A. aurescens LK9, A. aurescens and LK7. The results show that different bacterial species formed biofilm of different abundance and hydrolytic activitiy levels.