Recent advances in food-packing, pharmaceutical and biomedical applications of zein and zein-based materials.

Zein is a biodegradable and biocompatible material extracted from renewable resources; it comprises almost 80% of the whole protein content in corn. This review highlights and describes some zein and zein-based materials, focusing on biomedical applications. It was demonstrated in this review that the biodegradation and biocompatibility of zein are key parameters for its uses in the food-packing, biomedical and pharmaceutical fields. Furthermore, it was pointed out that the presence of hydrophilic-hydrophobic groups in zein chains is a very important aspect for obtaining material with different hydrophobicities by mixing with other moieties (polymeric or not), but also for obtaining derivatives with different properties. The physical and chemical characteristics and special structure (at the molecular, nano and micro scales) make zein molecules inherently superior to many other polymers from natural sources and synthetic ones. The film-forming property of zein and zein-based materials is important for several applications. The good electrospinnability of zein is important for producing zein and zein-based nanofibers for applications in tissue engineering and drug delivery. The use of zein's hydrolysate peptides for reducing blood pressure is another important issue related to the application of derivatives of zein in the biomedical field. It is pointed out that the biodegradability and biocompatibility of zein and other inherent properties associated with zein's structure allow a myriad of applications of such materials with great potential in the near future.

Application of a PLA/PBAT/Graphite sensor obtained by electrospinning on determination of 2,4,6-trichlorophenol.

The widespread use of pesticides requires effective detection and quantification tools to improve monitoring of environmental quality. Electrochemical sensors offer a fast and sensitive response, and can also be optimized by combining several constituents and techniques, including biodegradable materials, being useful in the determination of chemical agents from environmental samples. Here, we produced a polymer-based sensor for 2,4,6-trichlorophenol determination, through electrospinning of poly(lactic acid)/poly(butylene adipate-co-terephthalate) (PLA/PBAT) blend with graphite. The graphite-containing fibres were thermally treated and wetted in mineral oil, thus forming a paste, used as an electrode in the electrochemical sensor. The thermal analysis indicated a disorganization of the polymeric chains between the aromatic carbon chain of the PBAT polymer, resulting in a material with low enthalpy, lower crystallinity and greater thermal degradability after insertion of graphite in polymeric fibres. NIR spectra revealed changes related to the carbonyls of the polymeric ester groups. Cyclic voltammetry and square wave voltammetry techniques were applied to study the electrochemical behaviour of developed sensor. The thermal treatment of graphite-containing fibres increased the adhesion surface in which occurs the adsorption of the analyte on the electrode, which improved the peak current in the electrochemical tests. The PLA/PBAT/Graphite sensor applied to determination of 2,4,6-TCP presented the detection and quantification limits of 7.84 × 10-8 mol L-1 (0.0155 mg L-1) and 2.36 × 10-7 mol L-1 (0.0466 mg L-1) with a linearity response of 1.00 × 10-7 mol L-1 and 2.00 × 10-6 mol L-1 with correlation coefficient of 0.993 (r2).

Recent advances of Pluronic-based copolymers functionalization in biomedical applications.

The design of polymeric biocompatible nanomaterials for biological and medical applications has received special attention in recent years. Among different polymers, the triblock type copolymers (EO)x(PO)y(EO)x or Pluronics® stand out due its favorable characteristics such as biocompatibility, low tissue adhesion, thermosensitivity, and structural capacity to produce different types of macro and nanostructures, e.g. micelles, vesicles, nanocapsules, nanospheres, and hydrogels. However, Pluronic itself is not the "magic bullet" and its functionalization via chemical synthesis following biologically oriented design rules is usually required aiming to improve its properties. Therefore, this paper presents some of the main publications on new methodologies for synthetic modifications and applications of Pluronic-based nanoconstructs in the biomedical field in the last 15 years. In general, the polymer modifications aim to improve physical-chemical properties related to the micellization process or physical entrapment of drug cargo, responsive stimuli, active targeting, thermosensitivity, gelling ability, and hydrogel formation. Among these applications, it can be highlighted the treatment of malignant neoplasms, infectious diseases, wound healing, cellular regeneration, and tissue engineering. Functionalized Pluronic has also been used for various purposes, including medical diagnosis, medical imaging, and even miniaturization, such as the creation of lab-on-a-chip devices. In this context, this review discusses the main scientific contributions to the designing, optimization, and improvement of covalently functionalized Pluronics aiming at new strategies focused on the multiple areas of the biomedical field.

Films composed of white angico gum and chitosan containing chlorhexidine as an antimicrobial agent.

Anadenanthera colubrina, popularly known as white angico, is a species extensively cultivated in Brazil, mainly in the cerrado region, including the state of Piauí. This study examines the development of films composed of white angico gum (WAG) and chitosan (CHI) and containing chlorhexidine (CHX), an antimicrobial agent. The solvent casting method was used to prepare films. Different combinations and concentrations of WAG and CHI were used to obtain films with good physicochemical characteristics. Properties such as the in vitro swelling ratio, the disintegration time, folding endurance, and the drug content were determined. The selected formulations were characterised by scanning electron microscopy, Fourier-transform infrared spectroscopy, differential scanning calorimetry, thermogravimetric analysis, and X-ray diffraction, and the CHX release time and antimicrobial activity were evaluated. CHX showed a homogenous distribution in all CHI/WAG film formulations. The optimised films showed good physicochemical properties with 80% CHX release over 26 h, which is considered promising for local treatment of severe lesions in the mouth. Cytotoxicity tests of the films did not show toxicity. The antimicrobial and antifungal effects were very effective against the tested microorganisms.

Magnetic chitosan microgels: Synthesis, characterization, and evaluation of magnetic field effect over the drug release behavior.

Magnetic microgels based on chitosan, modified with glycidyl methacrylate (GMA) and activated with folic acid (FA), and cobalt ferrite nanoparticles, modified with GMA (GMACoFe2O4), were synthesized by emulsion polymerization. The size of the round-shaped microgels, with and without GMACoFe2O4, ranged from (1.62 ± 0.38) µm to (1.71 ± 0.61) µm, respectively. Their release behavior was evaluated in the presence and absence of a magnetic field (MF), using vitamin-B12 as a model drug. In the absence of MF, at pH 7.4, a fast release was observed, reaching the equilibrium after 30 min. In the MF presence, the alignment of the chains to it promoted an initial fast release, followed by a more controlled one, lasting for 50 min at pH 7.4. This type of release is attractive for the treatment of gastric wounds, which is improved by the presence of FA, conferring anti-oxidative and anti-secretory properties to the microgels.