RESUMO
Combination therapy using chemically distinct drugs has appeared as one of the promising strategies to improve anticancer treatment efficiency. In the present investigation, poly-(lactic-co-glycolic) acid (PLGA) nanoparticles electrostatically conjugated with polyethylenimine (PEI)-based co-delivery system for epirubicin and paclitaxel (PLGA-PEI-EPI-PTX NPs) has been developed. The PLGA-PEI-EPI-PTX NPs exhibited a monodispersed size distribution with an average size of 240.93 ± 12.70 nm as measured through DLS and 70.8-145 nm using AFM. The zeta potential of 41.95 ± 0.65 mV from -17.45 ± 2.15 mV further confirmed the colloidal stability and PEI modification on PLGA nanoparticles. Encapsulation and loading efficiency along with in vitro release of drug for nanoparticles were done spectrophotometrically. The FTIR analysis of PLGA-PEI-EPI-PTX NPs revealed the involvement of amide moiety between polymer PLGA and PEI. The effect of nanoparticles on the cell migration was also corroborated through wound healing assay. The MTT assay demonstrated that PLGA-PEI-EPI-PTX NPs exhibited considerable anticancer potential as compared to the naïve drugs. Further, p53 protein expression analysed through western blot showed enhanced expression. This study suggests that combination therapy using PLGA-PEI-EPI-PTX NPs represent a potential approach and could offer clinical benefits in the future for lung cancer patients.
Assuntos
Antineoplásicos/química , Portadores de Fármacos/química , Epirubicina/química , Nanopartículas/química , Paclitaxel/química , Copolímero de Ácido Poliláctico e Ácido Poliglicólico/química , Antineoplásicos/metabolismo , Antineoplásicos/farmacologia , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Liberação Controlada de Fármacos , Epirubicina/metabolismo , Epirubicina/farmacologia , Humanos , Concentração de Íons de Hidrogênio , Cinética , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/patologia , Paclitaxel/metabolismo , Paclitaxel/farmacologiaRESUMO
The present study aims at developing PGMD (poly-glycerol-malic acid-dodecanedioic acid)/curcumin nanoparticles based formulation for anticancer activity against breast cancer cells. The nanoparticles were prepared using both the variants of PGMD polymer (PGMD 7:3 and PGMD 6:4) with curcumin (i.e. CUR NP 7:3 and CUR NP 6:4). The size of CUR NP 7:3 and CUR NP 6:4 were found to be ~ 110 and 218 nm with a polydispersity index of 0.174 and 0.36, respectively. Further, the zeta potential of the particles was - 18.9 and - 17.5 mV for CUR NP 7:3 and CUR NP 6:4, respectively. The entrapment efficiency of both the nanoparticles was in the range of 75-81%. In vitro anticancer activity and the scratch assay were conducted on breast cancer cell lines, MCF-7 and MDA-MB-231. The IC50 of the nanoformulations was observed to be 40.2 and 33.6 µM at 48 h for CUR NP 7:3 and CUR NP 6:4, respectively, in MCF-7 cell line; for MDA-MB-231 it was 43.4 and 30.5 µM. Acridine orange/EtBr and DAPI staining assays showed apoptotic features and nuclear anomalies in the treated cells. This was further confirmed by western blot analysis that showed overexpression of caspase 9 indicating curcumin role in apoptosis.
Assuntos
Antineoplásicos/administração & dosagem , Curcumina/administração & dosagem , Portadores de Fármacos , Glicerol , Malatos , Nanopartículas , Polímeros , Apoptose/efeitos dos fármacos , Neoplasias da Mama/diagnóstico , Neoplasias da Mama/tratamento farmacológico , Neoplasias da Mama/mortalidade , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Portadores de Fármacos/síntese química , Portadores de Fármacos/química , Composição de Medicamentos , Liberação Controlada de Fármacos , Feminino , Glicerol/síntese química , Humanos , Cinética , Malatos/síntese química , Nanopartículas/química , Nanopartículas/ultraestrutura , Tamanho da Partícula , Polímeros/síntese química , Análise EspectralRESUMO
This study aims to determine the anticancer efficacy of diosgenin encapsulated poly-glycerol malate co-dodecanedioate (PGMD) nanoparticles. Diosgenin loaded PGMD nanoparticles (variants 7:3 and 6:4) were synthesized by the nanoprecipitation method. The synthesis of PGMD nanoparticles was systematically optimized employing the Box-Behnken design and taking into account the influence of various independent variables such as concentrations of each PGMD, diosgenin and PF-68 on the responses such as size and PDI of the particles. Mathematical modeling was done using the Quadratic second order modeling method and response surface analysis was undertaken to elucidate the factor-response relationship. The obtained size of PGMD 7:3 and PGMD 6:4 nanoparticles were 133.6 nm and 121.4 nm, respectively, as measured through dynamic light scattering (DLS). The entrapment efficiency was in the range of 77-83%. The in vitro drug release studies showed diffusion and dissolution controlled drug release pattern following Korsmeyer-Peppas kinetic model. Furthermore, in vitro morphological and cytotoxic studies were performed to evaluate the toxicity of synthesized drug loaded nanoparticles in model cell lines. The IC50 after 48 h was observed to be 27.14 µM, 15.15 µM and 13.91 µM for free diosgenin, PGMD 7:3 and PGMD 6:4 nanoparticles, respectively, when administered in A549 lung carcinoma cell lines.
Assuntos
Diosgenina/farmacologia , Sistemas de Liberação de Medicamentos/métodos , Glicerol/química , Malatos/química , Nanopartículas/química , Polímeros/química , Células A549 , Laranja de Acridina , Antineoplásicos/farmacologia , Apoptose , Sobrevivência Celular/efeitos dos fármacos , Portadores de Fármacos , Liberação Controlada de Fármacos , Difusão Dinâmica da Luz , Etídio/química , Humanos , Técnicas In Vitro , Concentração Inibidora 50 , Cinética , Luz , Modelos Teóricos , Tamanho da Partícula , Espalhamento de RadiaçãoRESUMO
Polyethylenimine (PEI) is one of the most efficient polycationic non-viral gene delivery vectors. Its efficiency and cytotoxicity depends on molecular weight, with the 25-kDa PEI being most efficient but accompanied with cytotoxicity. In the present study, enhancement in gene delivery efficiency along with reduction in cytotoxicity by attachment of guanidinium side group was explored. The hypothesis was that the guanidination would lead to the delocalization of charge present on primary amines of the polymer thereby leading to enhancement in gene delivery efficiency along with reduction in cytotoxicity. The polymer was guanidinated using O-methylisourea hemisulfate and the chemical linkage characterized by FTIR spectroscopy. The hydrodynamic diameter of guanidinated PEI-DNA complexes was determined using DLS. Subsequently, these complexes were used for DNA binding assay and zeta-potential measurements, taking native PEI as reference. Further, guanidinated PEI-DNA complexes were investigated for their gene delivery efficacy on HEK 293 cells. The hydrodynamic diameter of guanidinated PEI-DNA complexes was found to be in the range of 176-548 nm. As expected, the zeta potential values increased, on increasing the N/P ratios. It was found that guanidinated PEI had higher transfection efficiency at the majority of the N/P ratios tested as compared to commercially available transfecting agent lipofectin and native PEI itself. The toxicity of guanidinated PEI-DNA complexes was also reduced considerably in comparison to PEI polymer, as determined by MTT colorimetric assay. Out of the various derivatives prepared, gPEI 56% was found to be the most efficient in in vitro transfection.
Assuntos
DNA/administração & dosagem , Técnicas de Transferência de Genes , Guanidina/administração & dosagem , Polietilenoimina/administração & dosagem , Transfecção/métodos , Células Cultivadas , Humanos , PlasmídeosRESUMO
Polyethylenimine (750 kDa) has been derivatized to influence the proton sponge mechanism and hydrophobic-hydrophilic balance. The polymer was acylated using acid anhydrides of varying carbon chain length, followed by cross-linking with PEG-bis-P to form compact nanoparticles. The chemical linkages in the particles were characterized by FTIR and NMR spectroscopy. The hydrodynamic diameter of nanoparticles was found to be in the range of 83.5-124 nm. AFM imaging of native and DNA-loaded nanoparticles revealed highly compact and spherical shape. The positive surface charge on particles decreased with the increase in percentage of acylation and also on complexing with DNA. The buffering capacity of PEI was reduced considerably on preparing acylated nanoparticles. The nanoparticles formed stable complexes with DNA and higher weight ratios were required for formation of electro-neutral complexes. Further, these nanoparticles were investigated for their gene delivery efficacy on COS-1 cells. It was found that acylated PEI nanoparticles were 5-12-fold more efficient transfecting agents as compared to native PEI (750 kDa) and commercially available transfecting agent lipofectin. The MTT colorimetric assay revealed of considerable reduction in toxicity of acylated PEI nanoparticles as compared PEI. Of all the systems prepared, nanoparticles with 30% acylation using propionic anhydride were found to be the most efficient in in vitro transfection.
Assuntos
Anidridos/química , DNA/química , Nanopartículas , Polietilenoimina/química , Transfecção/métodos , Anidridos Acéticos/química , Acilação , Animais , Butiratos/química , Células COS , Sobrevivência Celular/efeitos dos fármacos , Chlorocebus aethiops , Reagentes de Ligações Cruzadas/química , DNA/metabolismo , Relação Dose-Resposta a Droga , Concentração de Íons de Hidrogênio , Luz , Espectroscopia de Ressonância Magnética , Microscopia de Força Atômica , Peso Molecular , Tamanho da Partícula , Fosfatidiletanolaminas/química , Polietilenoglicóis/química , Polietilenoimina/metabolismo , Polietilenoimina/toxicidade , Propionatos/química , Espalhamento de Radiação , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
The positive charge on PEI was partially shielded by forming ionic nanocomposites with a polysaccharide, alginic acid, in aqueous solution, bypassing tedious chemical synthesis. The content of alginic acid was varied systematically to obtain a series of nanocomposites. The nanocomposites were first characterized by assessing the surface charge (zeta potential), size (DLS) and morphology (AFM) followed by evaluation for their DNA interaction ability, cytotoxicity and transfection efficiency on various cell lines. The transfection efficiency of PEI-alginate (6.26%) nanocomposites improved dramatically (2-16-fold over native PEI) in all the cell lines studied. However, a decrease in transfection efficiency was observed on deviating from this optimal concentration of alginic acid in nanocomposites. Cytotoxicity of PEI-alginate/DNA complexes was nearly abolished on increasing the concentration of alginic acid in nanocomposites. PEI-alginate (6.26%) nanocomposites also delivered SiRNAs efficiently into mammalian cells, resulting in 80% suppression of GFP expression. The cellular uptake and endosomal escape of PEI-alginate nanocomposites and PEI were found to follow a similar route when transfection was carried out in presence of chloroquine, bafilomycin A1, cytochalasin B and methyl-beta-cyclodextrin. The results demonstrate a versatile vector that can be used for efficient cytoplasmic delivery of a broad range of nucleic acids.
Assuntos
Alginatos/administração & dosagem , DNA/administração & dosagem , Iminas/administração & dosagem , Nanoestruturas , Polietilenos/administração & dosagem , Transfecção/métodos , Animais , Células COS , Sobrevivência Celular/efeitos dos fármacos , Chlorocebus aethiops , Citocalasina B/farmacologia , Ácido Glucurônico/administração & dosagem , Ácidos Hexurônicos/administração & dosagem , Tamanho da Partícula , RNA Interferente Pequeno/administração & dosagemRESUMO
Two cross-linkers based on polyethylene glycol (PEG) (MW=6 and 8 kDa), were synthesized for self-assembling and formation of nanoparticles of branched, high molecular weight polyethylenimine (PEI). Cross-linking was realized in two ways, viz., ionic as well as covalent. Ionic cross-linking was accomplished by using PEG-bis (phosphate) whereas, the covalent one was achieved by using PEG-bis (p-nitrophenylcarbonate). A range of nanoparticles of PEI was prepared by varying the degree of cross-linking (i.e. the amount of cross-linkers used). PEI-PEG nanoparticles were characterized by dynamic light scattering and transmission electron microscopy and found to be in the range of approximately 18-75 nm (hydrodynamic radii) with almost uniform population. Subsequently, these particles were used for DNA binding assay and zeta-potential measurements, taking native PEI-PEG nanoparticles as reference. As expected, the zeta potential values decreased, on increasing the percentage of cross-linking as well as on complexation with DNA. Further, PEI-PEG nanoparticles were investigated for their transfecting efficacy on COS-1 cells. It was found that PEI-PEG nanoparticles were 5- to 16-fold more efficient as transfecting agents compared to lipofectin and PEI itself. The toxicity of PEI-PEG nanoparticles was found to be reduced considerably in comparison to PEI polymer, as determined by MTT colorimetric assay. Out of the various systems prepared, PEI-PEG8000 (5% ionic) nanoparticles were found to be the most efficient transfecting agent for in vitro transfection.
Assuntos
Nanoestruturas , Polietilenoimina/química , Transfecção/métodos , Animais , Células COS , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Chlorocebus aethiops , Reagentes de Ligações Cruzadas , DNA/administração & dosagem , DNA/química , Eletroquímica , Eritrócitos/efeitos dos fármacos , Excipientes , Hemólise/efeitos dos fármacos , Humanos , Microscopia Eletrônica de Transmissão , Peso Molecular , Tamanho da Partícula , Fosfatos/química , Polietilenoglicóis/químicaRESUMO
Polymeric nanoparticles of AADG cross-linked with MBA encapsulating water soluble macromolecules such as FITC-Dextran have been prepared in the reverse micellar system. The particles obtained were of >85nm in diameter which were highly monodisperse. An optically clear solution was obtained on redispersing these nanoparticles in aqueous buffer. Size and morphology of the particles remains the same on re-dispersing the lyophilized powder of these nanoparticles in aqueous buffer. The size dependency of the particles on the monomer and surfactant concentration was observed. The average size of the nanoparticles as obtained from DLS studies ranges from 74 to 114nm in case 0.06M AOT and 62-104nm in case of 0.1M AOT concentration. FITC-Dextran was entrapped into nanoparticles with high efficiency (>70%). The pH dependent release of the entrapped molecules from these nanoparticles was also studied. At pH 5.0 solution, approximately 43% of FITC-Dx was released and at pH 7.4 it was about 70%.
Assuntos
Sistemas de Liberação de Medicamentos/métodos , Nanopartículas/química , Polímeros/química , Acrilamidas/química , Acrilatos/química , Resinas Acrílicas/síntese química , Resinas Acrílicas/química , Preparações de Ação Retardada/síntese química , Preparações de Ação Retardada/química , Dextranos/administração & dosagem , Dextranos/química , Dextranos/farmacocinética , Portadores de Fármacos/síntese química , Portadores de Fármacos/química , Composição de Medicamentos/métodos , Fluoresceína-5-Isotiocianato/administração & dosagem , Fluoresceína-5-Isotiocianato/análogos & derivados , Fluoresceína-5-Isotiocianato/química , Fluoresceína-5-Isotiocianato/farmacocinética , Glucosamina/análogos & derivados , Glucosamina/química , Luz , Microscopia Eletrônica de Transmissão , Tamanho da Partícula , Polímeros/síntese química , Espalhamento de Radiação , Espectroscopia de Infravermelho com Transformada de Fourier , Succinatos/químicaRESUMO
CONTEXT: The size of nanoparticles plays a pivotal role in determining the gene delivery efficiency. OBJECTIVE: A focus on the studies done to investigate the effect of nanoparticles size on biological aspects of gene delivery. METHODS: A through literature survey has been done regarding studies done to investigate the effect of nanoparticles size on uptake, transfection efficiency and biodistribution has been cited in the present review. RESULTS AND CONCLUSION: The gene delivery efficacy may depend on conjugation of several factors such as the chemical structure of polymers, cell type, and nanoparticle size, composition and interaction with cells.
Assuntos
Técnicas de Transferência de Genes , Terapia Genética/métodos , Nanopartículas/química , Transgenes , Linhagem Celular , Sobrevivência Celular , DNA/genética , DNA/metabolismo , Endocitose/genética , Humanos , Nanopartículas/metabolismo , Tamanho da Partícula , Polietilenoimina/química , Polilisina/química , Distribuição Tecidual , Transferrina/químicaRESUMO
CONTEXT: Polymers have been largely explored for the preparation of nanoparticles due to ease of preparation and modification, large gene/drug loading capacity, and biocompatibility. Various methods have been adapted for the preparation and characterization of chitosan nanoparticles. OBJECTIVE: Focus on the different methods of preparation and characterization of chitosan nanoparticles. METHODS: Detailed literature survey has been done for the studies reporting various methods of preparation and characterization of chitosan nanoparticles. RESULTS AND CONCLUSION: Published database suggests of several methods which have been developed for the preparation and characterization of chitosan nanoparticles as per the application.
Assuntos
Quitina/química , Quitosana/química , Portadores de Fármacos/química , Nanopartículas/química , Polietilenoglicóis/química , Acetilação , Precipitação Química , Reagentes de Ligações Cruzadas/química , Portadores de Fármacos/uso terapêutico , Emulsões , Géis , Humanos , Microscopia/métodos , Nanopartículas/uso terapêutico , Nanopartículas/ultraestrutura , Tamanho da PartículaRESUMO
Small interfering RNAs (siRNAs) are rapidly emerging as new therapeutic tools for the treatment of some of the deadly diseases such as cancer. However, poor cellular uptake and instability in physiological milieu limit its therapeutic potential, hence there arises a need of a delivery system which can efficiently and repeatedly deliver siRNA to the target cells. Nanoparticles have shown immense potential as suitable delivery vectors with enhanced efficacy and biocompatibility. These delivery vectors are usually few nanometers in size, which not only protects siRNA against enzymatic degradation but also leads to tissue and cellular targeting. Nanoparticles prepared from various cationic polymers like polyethylenimine, and chitosan have been largely exploited as they bear several advantages such as, ease of manipulation, high stability, low cost and high payload. This review summarizes some of the recent patents on siRNA delivery employing polymer or lipid-based nano-vectors for therapeutic applications.
Assuntos
Nanopartículas/administração & dosagem , Nanopartículas/química , RNA Interferente Pequeno/administração & dosagem , Animais , Cátions/administração & dosagem , Cátions/química , Quitosana/química , Técnicas de Transferência de Genes/tendências , Humanos , Lipídeos/administração & dosagem , Lipídeos/química , Lipossomos , Camundongos , Neoplasias/genética , Neoplasias/terapia , Patentes como Assunto , Peptídeos/química , Polietilenoimina/química , RNA Interferente Pequeno/químicaRESUMO
Recent discovery of RNA interference (RNAi) technology for gene therapy has triggered explosive research efforts towards development of small interfering RNA (siRNA) as therapeutic modality for gene silencing. Owing to its large molecular weight (~13 kDa), polyanionic nature (~40 negative phosphate groups) and rapid enzymatic degradation, delivery of siRNA remains an unresolved issue. Hence, there arises a need of an appropriate delivery vector to overcome the intrinsic, poor intracellular uptake and limited in vitro and in vivo stability. Amongst the various non-viral delivery vectors, the application of polymeric vectors such as polyethylenimine (PEI) or its derivatives has attracted much attention due to its high transfection efficiency and ease of manipulation. PEI has been extensively investigated for DNA delivery, only recently this polymer has been employed for siRNA delivery. This review will focus on studies done on PEI to deliver siRNA, with emphasis on the targeted, self-assembled polymeric nanoparticles with promising potential to evolve as therapeutic tool in gene therapy.
Assuntos
Iminas/química , Polietilenos/química , Interferência de RNA , RNA Interferente Pequeno/administração & dosagem , Animais , Inativação Gênica , Terapia Genética/métodos , Humanos , Nanopartículas , Polímeros/química , TransfecçãoRESUMO
siRNA are a rapidly emerging class of new therapeutic molecules for the treatment of inherited and acquired diseases. However, poor cellular uptake and instability in physiological conditions limits its therapeutic potential, hence a need to develop a delivery system that can protect and efficiently transport siRNA to the target cells has arisen. Nanoparticles have been proposed as suitable delivery vectors with reduced cytotoxicity and enhanced efficacy. These delivery vectors form condensed complexes with siRNA which, in turn, provides protection to siRNA against enzymatic degradation and further leads to tissue and cellular targeting. Nanoparticles derived from polymers, such as chitosan and polyethylenimine have found numerous applications owing to ease of manipulation, high stability, low cost and high gene carrying capability. This article focuses on various aspects of nanomedicine based siRNA delivery with emphasis on targeted delivery to tumors.
Assuntos
Nanomedicina/métodos , Nanopartículas/administração & dosagem , Nanopartículas/química , RNA Interferente Pequeno/administração & dosagem , Animais , Quitosana/química , Humanos , Modelos Biológicos , Modelos Teóricos , Neoplasias/genética , Neoplasias/metabolismo , Neoplasias/terapia , Polietilenoimina/química , Polímeros/química , RNA Interferente Pequeno/químicaRESUMO
The design and development of nucleic acids based therapeutics for the treatment of diseases arising from genetic abnormalities has made significant progress over the past few years. Advances in synthetic oligonucleotide chemistry resulted in synthesis of nucleic acids that are relatively stable in in vivo environments. However, cellular targeting and intracellular delivery of nucleic acids still remains a challenge. Further, development of nucleic acids based therapeutics depends on the progress of safe and effective carriers for systemic administration. Cationic vectors with diminished cytotoxicity and enhanced efficacy are rapidly emerging as systems of choice. These vectors protect nucleic acids from enzymatic degradation by forming condensed complexes along with targeted tissue and cellular delivery. During the past few years, myriad of reports have appeared reporting delivery of nucleic acids mediated by nanocarriers. This review provides an overview of various nanocarriers employed for in vitro and in vivo delivery of therapeutically relevant nucleic acids e.g., DNA, siRNA, LNA, PNA etc.
Assuntos
Nanocápsulas/química , Ácidos Nucleicos/administração & dosagem , Ácidos Nucleicos/uso terapêutico , Animais , Cátions/química , DNA Catalítico/administração & dosagem , Sistemas de Liberação de Medicamentos , Humanos , Nanocápsulas/administração & dosagem , Nanotecnologia , Oligodesoxirribonucleotídeos Antissenso/administração & dosagem , Oligonucleotídeos/administração & dosagem , Ácidos Nucleicos Peptídicos/administração & dosagem , Plasmídeos/administração & dosagem , Polímeros/química , RNA Interferente Pequeno/administração & dosagemRESUMO
Degradation of mRNA by RNA interference is one of the most powerful and specific mechanism for gene silencing. Owing to this property, siRNAs are emerging as promising therapeutic agents for the treatment of inherited and acquired diseases, as well as research tools for the elucidation of gene function in both health and disease. Here we have explored the potential of polyethylenimine (PEI) to deliver siRNA to mammalian cells. Nanoparticles of PEI were prepared by acylating PEI with propionic anhydride followed by cross-linking with polyethylene glycol-bis(phosphate). The nanoparticles size as revealed by DLS studies was found to be approximately 110 nm and AFM investigations showed spherical and compact complexes with an average size of 100 nm. For electro-neutralization of negative charge of siRNA higher amount of nanoparticles was required as compared to native PEI. The siRNA delivery efficiency of nanoparticles was assessed by using siRNA against gene coding for green fluorescent protein (GFP). The gene silencing efficiency of PEI nanoparticles was found to be comparable to commercially available transfecting agent Lipofectin but with reduced cytotoxicity.