RESUMO
A complex system, based on a dendrimer solubilized in the aqueous core of water-in-oil microemulsion, may combine the advantages of both dendrimers and microemulsions to provide better control of drug release. We report for the first time the use of EPR technique to determine the effect of solubilized dendrimer on the structure of the microemulsion. The solubilized poly(propyleneimine) (PPI-G2) interacts with sodium bis(2-ethylhexyl) sulfosuccinate (AOT). EPR analysis provided information on polarity, microviscosity, and molecular order of the systems. Polarity and microviscosity increased from unloaded water-in-oil microemulsion to the system loaded with 0.2 wt % PPI-G2, but remained unchanged with higher PPI-G2 loads. The degree of order also increased with 0.2 wt % PPI-G2 with only minor additional increase with larger quantities (25 wt %) of PPI-G2. Variations in pH only slightly affected the structure of microemulsion in the absence and presence of the loaded dendrimers. Aliphatic oils with longer lipophilic chains enhanced the structural order of the microemulsion. On increasing water content, polarity and degree of order increased. PPI-G2 dendrimer in small loads is attracted by the negatively charged AOT and thus intercalates in the interface of the droplets. Yet, at higher PPI-G2 loads, the excess molecules are solubilized in the water core.
Assuntos
Dendrímeros/química , Nanopartículas/química , Óleos/química , Polipropilenos/química , Água/química , Espectroscopia de Ressonância de Spin Eletrônica , Emulsões/química , Modelos Moleculares , Estrutura Molecular , Tamanho da Partícula , Solubilidade , Propriedades de SuperfícieRESUMO
Advanced drug delivery nanosystems (aDDnSs) combining liposomal and dendritic materials have only recently appeared in the research field of drug delivery. The nature and localization of the interactions between the components of such systems are not yet fully described. In this study, liposomes are combined with hyperbranched polyesters for the development of new aDDnSs. The polymer-lipid interactions along with their dependence on the polyesters pseudogeneration number and the liposomal lipid composition have been examined. The results indicate that the interaction between the materials takes place in the headgroup region, where H-bonds between the polymers terminal hydroxyls and the phospholipids phosphate moiety are formed. Due to the polymers' compact imperfect structure, which varies with pseudogeneration number, no linear trends are observed with increasing pseudogeneration number. Moreover, it is shown that high percentages of cholesterol in the lipid bilayer affect the penetration of the polymers in the headgroup region.
Assuntos
Lipossomos/química , Nanotecnologia , Polímeros/química , Ligação de Hidrogênio , Bicamadas Lipídicas/química , Poliésteres/química , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
The present work investigates, for the first time, a system comprising a dendrimer incorporated into the water core of water-in-oil (W/O) microemulsion (ME). A second generation (G-2) poly(propyleneimine) dendrimer (PPI) was solubilized into W/O ME composed of AOT (sodium bis(2-ethylhexyl)sulfosuccinate), heptane, and water. Such a model system possessing the benefits of both dendrimers and ME, can potentially offer superior control of drug administration. The localization of PPI within the system, its specific interactions with the components of the carrier, and its effect on the ME structure was explored by SAXS, DSC, ATR-FTIR, and electrical conductivity measurements. Considerable water binding by PPI, accompanied by partial dehydration of AOT polar heads, was detected by ATR-FTIR and DSC analysis, suggesting that PPI acted as a "water pump". In addition, SAXS measurements showed periodicity increase and disordering of the droplets. Hence, localization of PPI within the core and interfacial regions of the droplets was assumed. Direct electrostatic interactions between PPI and the sulfonate group were not noticed, since the dendrimer molecules were mostly not protonated in the current basic environment at pH 12. However, slight hydrogen bonding between PPI and the S=O groups allowed the dendrimer to behave as a "spacer" between sodium and sulfonate ions. This affected the electrical conductivity behavior of the system, revealing that PPI favored the percolation process. Most likely, PPI decreased the rigidity of the interfacial layer, facilitating the diffusion of sodium ions through the channels. The characterized model system can be advantageously utilized to design specific delivery vehicles, allowing administration of dendrimers as a therapeutic agent from host MEs.