Full-Chain FeCl3 Catalyzation Is Sufficient to Boost Cellulase Secretion and Cellulosic Ethanol along with Valorized Supercapacitor and Biosorbent Using Desirable Corn Stalk.

Cellulosic ethanol is regarded as a perfect additive for petrol fuels for global carbon neutralization. As bioethanol conversion requires strong biomass pretreatment and overpriced enzymatic hydrolysis, it is increasingly considered in the exploration of biomass processes with fewer chemicals for cost-effective biofuels and value-added bioproducts. In this study, we performed optimal liquid-hot-water pretreatment (190 °C for 10 min) co-supplied with 4% FeCl3 to achieve the near-complete biomass enzymatic saccharification of desirable corn stalk for high bioethanol production, and all the enzyme-undigestible lignocellulose residues were then examined as active biosorbents for high Cd adsorption. Furthermore, by incubating Trichoderma reesei with the desired corn stalk co-supplied with 0.05% FeCl3 for the secretion of lignocellulose-degradation enzymes in vivo, we examined five secreted enzyme activities elevated by 1.3-3.0-fold in vitro, compared to the control without FeCl3 supplementation. After further supplying 1:2 (w/w) FeCl3 into the T. reesei-undigested lignocellulose residue for the thermal-carbonization process, we generated highly porous carbon with specific electroconductivity raised by 3-12-fold for the supercapacitor. Therefore, this work demonstrates that FeCl3 can act as a universal catalyst for the full-chain enhancement of biological, biochemical, and chemical conversions of lignocellulose substrates, providing a green-like strategy for low-cost biofuels and high-value bioproducts.

10-Year Results of Therapeutic Ratio by Intensity-Modulated Radiotherapy Versus Two-Dimensional Radiotherapy in Patients with Nasopharyngeal Carcinoma.

BACKGROUND: The purpose of this study was to verify 10-year results of survival and late toxicities and assess the ultimate therapeutic ratio of intensity-modulated radiotherapy (IMRT) versus two-dimensional radiotherapy (2DRT) in patients with nasopharyngeal carcinoma (NPC). MATERIALS AND METHODS: We retrospectively reviewed the data from 1,276 patients with nonmetastatic NPC who received IMRT or 2DRT from January 2003 to December 2006. RESULTS: Of the 1,276 patients, 512 were treated with IMRT and 764 with 2DRT. Median follow-up was 115 months. At 10 years, the IMRT group demonstrated significantly better results than the 2DRT group in local failure-free survival (L-FFS; 90% vs. 84%; hazard ratio [HR], 0.57, 95% confidence interval [CI], 0.40-0.81; p = .001), failure-free survival (FFS; 69% vs. 58%; HR, 0.69, 95% CI, 0.57-0.83; p < .001), and overall survival (OS; 75% vs. 63%; HR, 0.62, 95% CI, 0.51-0.77; p < .001). Subgroup multivariate analyses showed that radiotherapeutic technique (IMRT vs. 2DRT) remained an independent prognostic factor for L-FFS in the T1 subgroup (HR, 0.30; 95% CI, 0.11-0.80; p = .02); for FFS in the stage II subgroup (HR, 0.42; 95% CI, 0.24-0.73; p = .002); and for OS in the stage I (HR, 0.20; 95% CI, 0.04-0.96; p = .04), stage II (HR, 0.39; 95% CI, 0.21-0.75; p = .004), and stage IVA-B (HR, 0.74, 95% CI, 0.56-0.98; p = .04) subgroups. The incidence of grade 3-4 temporal lobe necrosis, cranial neuropathy, eye damage, ear damage, neck soft tissue damage, trismus, and dry mouth was significantly lower in the IMRT group than in the 2DRT group. CONCLUSION: IMRT demonstrated an improved ultimate therapeutic ratio compared with 2DRT in patients with NPC after a 10-year follow-up, with significant improvement of L-FFS, FFS, and OS and decrease in most late toxicities. IMPLICATIONS FOR PRACTICE: The ultimate therapeutic ratio of intensity-modulated radiotherapy versus two-dimensional radiotherapy in patients with nasopharyngeal carcinoma is unclear. In this retrospective study of 1,276 patients with nonmetastatic nasopharyngeal carcinoma with a follow-up of 115 months, intensity-modulated radiotherapy demonstrated an improved ultimate therapeutic ratio compared with two-dimensional radiotherapy, with significant improvement of local failure-free survival, failure-free survival, and overall survival and decrease in most late toxicities and noncancer deaths. However, distant control remains insufficient with this treatment modality.

Robust and Reversible Vapoluminescent Organometallic Copper Polymers.

Emissive organometallic polymers are an important class of functional materials characterized by the combined photoluminescent features of organometallic molecules and the properties of traditional polymers. In this work, the emissive organometallic complex, [CuBr(PPh3 )2 (4-methylpyridine)], is successfully, mechanically ground into a random copolymer built on 4-(diphenylphosphino)styrene (DPVP) and n-butyl acrylate (BA) monomers. The resultant hybrid materials successfully inherit the emissive centers, and are significantly reinforced by the copper complexes as chemical crosslinkers in the polymeric continuum. These organometallic polymers are also proved to have excellent vapoluminescent properties, exhibiting unique responses to many organic solvent vapors, reflecting their rapid loss and recovery of photoluminescence. Mechanically robust and flexible films prepared with these organometallic Cu(I)-polymers are tested as recoverable sensors for hazardous volatile chemical compounds (VOCs) such as toluene, acetone, chloroform, and dichloromethane, and the low limits of detection (LOD) can reach as low as 1 × 10-3 -8 × 10-3 mg L-1 (0.2-3.3 ppmV, parts per million-volume) for various VOCs. This work sheds lights on the design and fabrication of organometallic polymers for advanced applications.

Investigation of the Source of Snoring Sound by Drug-Induced Sleep Nasendoscopy.

OBJECTIVE: To investigate the source of snoring sound in patients with simple snoring (SS) and different degrees of obstructive sleep apnea syndrome (OSAS) in order to provide a basis for the surgical treatment of snoring. METHODS: Fifty-two patients with either SS or OSAS (with an apnea-hypopnea index ≤40) underwent drug-induced sleep nasendoscopy (DISN). Vibration sites in the pharyngeal cavity were observed. RESULTS: Vibration of the soft palate, pharyngeal lateral wall, epiglottis, and tongue base appeared in 100, 53.8, 42.3, and 26.9% of the patients, respectively. The source of snoring sound was divided into two types: palatal fluttering only (type I) and multisite vibration (type II). The latter was divided into 3 subtypes: palatal fluttering with epiglottis vibration (type IIa), palatal fluttering with lateral wall vibration (type IIb), and palatal fluttering with vibration of the lateral wall, epiglottis, and tongue base together (type IIc). The distribution of type I snoring was the highest in SS patients. Type IIb was more common in patients with medium and severe OSAS. Type IIc was most common in patients with severe OSAS. CONCLUSION: The source of snoring sound is diverse, with SS and OSAS patients showing different features. DISN is a very effective method of identifying the snoring source.

Upgraded cellulose and xylan digestions for synergistic enhancements of biomass enzymatic saccharification and bioethanol conversion using engineered Trichoderma reesei strains overproducing mushroom LeGH7 enzyme.

Crop straws provide enormous lignocellulose resources transformable for sustainable biofuels and valuable bioproducts. However, lignocellulose recalcitrance basically restricts essential biomass enzymatic saccharification at large scale. In this study, the mushroom-derived cellobiohydrolase (LeGH7) was introduced into Trichoderma reesei (Rut-C30) to generate two desirable strains, namely GH7-5 and GH7-6. Compared to the Rut-C30 strain, both engineered strains exhibited significantly enhanced enzymatic activities, with ß-glucosidases, endocellulases, cellobiohydrolases, and xylanase activities increasing by 113 %, 140 %, 241 %, and 196 %, respectively. By performing steam explosion and mild alkali pretreatments with mature straws of five bioenergy crops, diverse lignocellulose substrates were effectively digested by the crude enzymes secreted from the engineered strains, leading to the high-yield hexoses released for bioethanol production. Notably, the LeGH7 enzyme purified from engineered strain enabled to act as multiple cellulases and xylanase at higher activities, interpreting how synergistic enhancement of enzymatic saccharification was achieved for distinct lignocellulose substrates in major bioenergy crops. Therefore, this study has identified a novel enzyme that is active for simultaneous hydrolyses of cellulose and xylan, providing an applicable strategy for high biomass enzymatic saccharification and bioethanol conversion in bioenergy crops.

Cellulose de-polymerization is selective for bioethanol refinery and multi-functional biochar assembly using brittle stalk of corn mutant.

As lignocellulose recalcitrance principally restricts for a cost-effective conversion into biofuels and bioproducts, this study re-selected the brittle stalk of corn mutant by MuDR-transposon insertion, and detected much reduced cellulose polymerization and crystallinity. Using recyclable CaO chemical for biomass pretreatment, we determined a consistently enhanced enzymatic saccharification of pretreated corn brittle stalk for higher-yield bioethanol conversion. Furthermore, the enzyme-undigestible lignocellulose was treated with two-step thermal-chemical processes via FeCl2 catalysis and KOH activation to generate the biochar with significantly raised adsorption capacities with two industry dyes (methylene blue and Congo red). However, the desirable biochar was attained from one-step KOH treatment with the entire brittle stalk, which was characterized as the highly-porous nanocarbon that is of the largest specific surface area at 1697.34 m2/g and 2-fold higher dyes adsorption. Notably, this nanocarbon enabled to eliminate the most toxic compounds released from CaO pretreatment and enzymatic hydrolysis, and also showed much improved electrochemical performance with specific capacitance at 205 F/g. Hence, this work has raised a mechanism model to interpret how the recalcitrance-reduced lignocellulose is convertible for high-yield bioethanol and multiple-function biochar with high performance.

Kawasaki disease with peritonsillar abscess as the first symptom: A case report.

BACKGROUND: Kawasaki disease (KD), also known as mucocutaneous lymph node syndrome, is an acute, self-limiting vasculitis of unknown aetiology that mainly involves the medium and small arteries and can lead to serious cardiovascular complications, with a 25% incidence of coronary artery aneurysms. Periton-Sillar abscesses are a rare symptom of KD and is easily misdiagnosed at its early stages. CASE SUMMARY: A 5-year-old boy who presented to a community hospital with a 3-d fever, difficulty in opening his mouth, and neck pain and was originally treated for throat infection without improvement. On the basis of laboratory tests, ultrasound of submandibular and superficial lymph nodes and computed tomography of the neck, the clinician diagnosed the periamygdala abscess and sepsis that did not resolve after antibiotic therapy. On the fifth day of admission, the child developed conjunctival congestion, prune tongue, perianal congestion and desquamation, and slightly stiff and swollen bunions on both feet. A diagnosis of KD was reached with complete remission after intravenous immunoglobulin treatment. CONCLUSION: Children with neck pain, lymph node enlargement, or airway obstruction as the main manifestations are poorly treated with intravenous broad-spectrum antibiotics. Clinicians should not rush invasive operations such as neck puncture, incision, and drainage and should be alert for KD when it cannot be explained by deep neck space infection and early treatment with aspirin combined with gammaglobulin.

Braided scaffolds with polypyrrole/polydopamine/hydroxyapatite coatings with electrical conductivity and osteogenic properties for bone tissue engineering.

When impaired bones are grafted with bone scaffolds, the behaviors of osteoblast are dependent on the implant materials and surface morphology. To this end, we modulated the surface morphology of scaffolds that promote cell growth. In this study, ice-template and spraying method methods are employed to coat different proportions of PDA and PPy over the PLA/PVA weaving scaffolds, after which HA is Coated over via the electrochemical deposition, forming weaving scaffolds with electrically conductive PDA/PPy/HA coating. The test results indicate that with a PPy/PDA concentration ratio is 30, the PPy particles are more uniformly distributed on the fiber surface. The scaffolds are wrapped in a HA coating layer with a high purity, and calcium and phosphorus elements are evenly dispersed with a Ca/P ratio being 1.69. Owing to the synergistic effect between PDA and PPy coating, the scaffolds demonstrate excellent electrochemical stability and electrochemical activity. The biological activity of the scaffold increased to 274.66% under electrical stimulation. The new thinking proposed by this study extends the worth of applying textile structure to the medical field, the application of which highly increases the prospect of bone tissue engineering.

Intermittent ultrasound retains cellulases unlock for enhanced cellulosic ethanol with high-porosity biochar for dye adsorption using desirable rice mutant straw.

In this study, optimal ultrasound pretreatment was performed with recalcitrance-reduced rice mutant straw to effectively extract lignin and hemicellulose for improved cellulose accessibility. Intermittent ultrasound-assistant enzymatic hydrolyses were followed to maintain more cellulases unlock and less cellulose surface block with lignin for raised hexose yield at 81 % (% cellulose) and bioethanol concentration at 9.9 g/L, which was higher than those of other mechanical pretreatments as previously conducted. Using all enzyme-undigestible lignocellulose residues, this work generated the biochar with the highest porosity (SBET at 2971 m2/g) among all biomass-based biochar obtained from previous studies. Furthermore, the biochar were respectively examined with high adsorption capacity for Congo red and methylene blue at 7946 mg/g and 861 mg/g. Therefore, this study has demonstrated a green-like process technology for high-yield bioethanol and high-porosity biochar with full biomass utilization by integrating optimal ultrasound pretreatment with intermittent ultrasound-assistant enzymatic hydrolyses of recalcitrance-reduced lignocellulose in crop straws.

Zein nanospheres assisting inorganic and organic drug combination to overcome stent implantation-induced thrombosis and infection.

The complication of stent implantation is the biggest obstacle to the success of its clinical application. In this study, we developed a combination way of 3D printing and the coating technique for preparation of functional polyurethane stents against stent implantation-induced thrombosis and postoperative infection. SEM, XPS, static water contact angle, and XRD demonstrated that the functional polyurethane stent had a 37 µm-thickness membrane composed of zein nanospheres (250-350 nm). Meanwhile, ZnO nanoparticles were encapsulated in zein nanospheres while heparin was adsorbed on the surface, causing 97.1 ± 6.4 % release of heparin in 120 min (first-order kinetic model) and 62.7 ± 5.6 % release of Zn2+ in 9 days (Korsmeyer-Peppas model). The mechanical analysis revealed that the functional polyurethane stents had about 8.61 MPa and 2.5 MPa tensile strength and bending strength, respectively. The in vitro biological analysis showed that the functional polyurethane stents had good EA.hy926 cells compatibility (97.9 ± 3.8 %), anti-coagulation response (comparable plasma protein, platelet adhesion and suppressed clotting) and sustained antibacterial activities by comparison with the bare polyurethane stent. The preliminary evaluation by rabbit ex vivo carotid artery intervention experiment demonstrated that the functional polyurethane stents could maintain blood circulation under the continuous stresses of blood flow. Meanwhile, the detailed data from the simulated implant infection experiment in vivo showed the functional polyurethane stents could effectively reduce microbial infection by 3-6 times lower and improve fibrosis and macrophage infiltration.

Silk fibroin/polycaprolactone-polyvinyl alcohol directional moisture transport composite film loaded with antibacterial drug-loading microspheres for wound dressing materials.

Drug delivery technology can prevent wound infection and inflammatory reactions and accelerate wound healing and quality. In this paper, we propose preparing a multifunctional medical dressing to meet the various needs of people for dressing. A multi-layered composite nanofiber membrane was constructed using silk fibroin as the substrate, and mesoporous silica nanoparticles (MSN) with high adsorption properties were first prepared and then electrosprayed on silk fibroin (SF)/chitosan (CS) microspheres to form MSN-SF/CS microspheres with uniform distribution. Then the MSN-SF/CS microspheres were sprayed on the silk fibroin (SF)/polycaprolactone (PCL)-polyvinyl alcohol (PVA) unidirectional water-conducting composite nanofiber membrane. The test results showed that the encapsulation rate of bovine serum albumin (BSA) by MSN-SF/CS drug-loaded microspheres was 65.53% and the cumulative release rate in vitro was 54.46%. The results of in vitro experiments also showed its good antibacterial effect and good biocompatibility. To eliminate excess wound exudate and reduce inflammation, the cumulative unidirectional transport capacity (AOTC) of 651.75% was achieved by spraying the microspheres on an SF/PCL- PVA unidirectional water conductive composite membrane. This study could stimulate and promote the use of additional wound healing biomaterials in clinical medicine.

Recent progress of redox-responsive polymeric nanomaterials for controlled release.

Redox-responsive polymeric nanomaterials (PNMs) have been attractive research targets for drug delivery systems because disturbed levels of redox molecules are associated with the progression of various diseases. To enable PNMs to target biorelevant redox molecules, including reactive oxygen species (ROS), glutathione (GSH) and hydrogen sulfide (H2S), appropriate responsive moieties have to be installed within the polymer structure. Upon application of redox stimuli, redox-responsive PNMs undergo structural changes to release encapsulated payloads. Chalcogen ether, thioketal and arylboronic ester have been widely incorporated into the structure of ROS-responsive PNMs. While disulfide is commonly utilized in GSH-responsive PNMs, azide is a newly explored responsive motif targeting H2S selectively. Diselenide, on the other hand, is a group susceptible to both oxidative and reducing conditions and therefore it has been exploited in dual redox-responsive PNMs. Here, we review PNMs, mainly reported in the last four years, that contain these redox-responsive moieties for controlled payload release.

Effects of clay minerals on the transport of nanoplastics through water-saturated porous media.

Clay minerals are important constituents of porous media. To date, only little is known about the transport and retention behavior of nanoplastics in clay-containing soil. To investigate the effects of clay minerals on the mobility of nanoplastics in saturated porous media, polystyrene nanoplastics (PS-NPs) were pumped through columns packed with sand and clay minerals (kaolinite and illite) at different pH and ionic strengths (IS). Mobility of PS-NPs decreased with increasing clay content attributed to physical straining effects (smaller pore throats and more complex flow pathways). Variations in pH and IS altered the surface charges of both PS-NPs and porous media and thus affecting the interaction energy. An increase of IS from 10 mM to 50 mM NaCl decreased the maximum energy barrier and secondary minimum from 142 KBT to 84 KBT and from -0.1 KBT to -0.72 KBT, respectively. Thus, the maximum C/C0 ratio decreased from ~51% to ~0% (pH 5.9, 3% kaolinite). Among the two clay minerals, kaolinite showed a stronger inhibitory effect on PS-NPs transport compared to illite. For instance, at the same condition (3% clay content, pH 5.9, 10 mM NaCl), the (C/C0)max of PS-NPs in kaolinite was ~51%, while for illite, it was ~77%. The difference in transport inhibition was mainly attributed to amphoteric sites on the edges of kaolinite which served as favorable deposition sites at pH 5.9 (pHpzc-edge is ~2.5 for illite and ~6.5 for kaolinite). Besides, the morphology of kaolinite was more complex than illite, which may retain more PS-NPs in kaolinite. Results and conclusions from the study will provide some valuable insights to better understand the fate of NPs in the soil-aquifer system.

Two-step strategy for constructing hierarchical pore structured chitosan-hydroxyapatite composite scaffolds for bone tissue engineering.

Chitosan (CS) combined with hydroxyapatite (HA) was injected into a composite braid, and a hierarchical pore structure scaffold was obtained by freeze drying and cold atmospheric plasma (CAP) technology. The CS/HA/braid scaffold with hierarchical pore structure was analyzed and characterized by scanning electronic microscopy, Fourier transform infrared spectroscopy, true color confocal microscopy, improved liquid replacement method, and phosphate buffer solution immersion. The mechanical properties and degradation ability of the scaffold were evaluated through compression test and degradation test. Results showed that HA addition endowed the core of the scaffold with macroscopic pore sizes of 80-180 µm, and CAP treatment endowed the shell of the scaffold with microscopic pore sizes ≤10 µm. All scaffolds exhibited high porosity and swelling rates of ≥80 % and ≥300 %, respectively. The scaffold with a hierarchical pore structure had good mechanical properties and twice the degradation rate. In addition, the treated scaffold precipitated intact spherical HA crystals. Under the synergistic effect of HA and CAP treatment, scaffolds achieved 277.6 % cell viability compared with pure CS scaffold. Overall, this method was feasible for preparing bone scaffolds with hierarchical pore structure for potential bone tissue engineering.

Enhanced adsorption capacity of MgO/N-doped active carbon derived from sugarcane bagasse.

MgO/N-doped active carbon (Mg/N-C) derived from sugarcane bagasse was prepared for the removal of methyl orange (MO). Mg/N-C composites presented the better adsorption capacity than that of active carbon and N-doped active carbon, of which the maximum adsorption capacity of 2-Mg/N-C-b2 for the MO removal is 384.61 mg g-1. The effects of MgO dosage, N-doped content, pyrolysis temperature, pH value, inorganic ions and solution temperature on the adsorption performance of Mg/N-C composites in the MO removal were investigated in detail. The pseudo-second order model and Langmuir isotherm model fitted well with the adsorption kinetics and isotherms of Mg/N-C. The rate-determining step was the boundary diffusion and intra-particle diffusion. The adsorption process of 2-Mg/N-C-b2 was a spontaneous and physisorption process.

Highly responsive and rapid hydrogen peroxide-triggered degradation of polycaprolactone nanoparticles.

We synthesized an oxidation-responsive polycaprolactone (O-PCL) bearing pendant arylboronic esters as H2O2-responsive motifs. H2O2 induces fast depolymerization of O-PCL within days. Nanoparticles formulated from O-PCL disintegrate and release payload in response to concentrations of H2O2 (50 µM) that are relevant to human disease.

In situ growth polydopamine decorated polypropylen melt-blown membrane for highly efficient oil/water separation.

The removal of organic pollutants from water is highly desired because of the development of industrial and social economy. Superhydrophilic and underwater superoleophobic membranes are emerging materials for effective oil/water separation. In this paper, superhydrophilic and underwater superoleophobic polypropylene (PP) melt-blown membranes were prepared through melt-blown and in situ growth method, achieving highly efficient oil/water separation. After in situ growth, polydopamine (PDA) grows on the surface of PP fibers, and the addition of coupling agent (3-aminopropyltriethoxysilane, APTES) can improve the stability of the membrane in harsh environments (1 M HCl, 1 M NaOH, 1 M NaCl). The PDA/APTES@PP membrane could dramatically enhance the wetting (water contact angle ∼0, underwater oil contact angle∼154°) compare with the pristine PP melt-blown membrane (water contact angle ∼130°, underwater oil contact angle ∼0). Moreover, the filtration performance is at a high level (∼99%). The behaviors are comparable or even superior to the typical reported results in the references (such as the mussel-inspired superhydrophilic PVDF membrane and copper mesh). This method provides a facile route to prepared multi-functional membrane for highly efficiency oil/water separation and industrial oily wastewater remediation.

Reduction in murine acute GVHD severity by human gingival tissue-derived mesenchymal stem cells via the CD39 pathways.

Human gingival tissue-derived mesenchymal stem cells (GMSCs) present an accessible source of mesenchymal stem cells (MSCs) for treating autoimmune diseases. Here we show that human GMSCs can prevent and treat acute graft-versus-host disease (GVHD) in two different mouse models. Our results indicate that besides exhibiting suppressive function in vitro and in vivo, GMSCs may also regulate the conversion of Tregs to Th1 and/or Th17-like cells, as well as stabilize Foxp3 expression. Furthermore, GMSC-mediated prevention of acute GVHD was dependent on CD39 signaling that play an important role in the function and stability of Tregs. Finally, we also observed stronger protective ability of GMSCs with greater expansion ability compared with BMSCs or ASCs. These results indicate that human GMSCs have the potential to be used to treat GVHD.

Comparison of therapeutic effect between percutaneous vertebroplasty and kyphoplasty on vertebral compression fracture.

OBJECTIVE: To compare the clinical efficacy of percutaneous vertebroplasty (PVP) with percutaneous kyphoplasty (PKP) in the treatment of vertebral compression fracture (VCF). METHODS: Ninety-eight patients with VCF were treated by PVP (n=42) or PKP (n=56). The anterior midline and posterior heights of vertebrae body, preoperative and postoperative visual analogue scale (VAS), operation time and amount of blood loss were compared between 2 groups. RESULTS: There was statistical difference in vertebral height between two groups (P < 0.01). No significant difference was seen in VAS, operation time and blood loss between two groups (P < 0.05). CONCLUSIONS: PKP and PVP have the similar therapeutic efficacy in treatment of VCF with minimal invasion, less operation time and blood loss. However, PKP is superior in the recovery of vertebral height.

Effect of scaffold morphology and cell co-culture on tenogenic differentiation of HADMSC on centrifugal melt electrospun poly (L­lactic acid) fibrous meshes.

Engineered tendon grafts offer a promising alternative for grafting during the reconstruction of complex tendon tears. The tissue-engineered tendon substitutes have the advantage of increased biosafety and the option to customize their biochemical and biophysical properties to promote tendon regeneration. In this study, we developed a novel centrifugal melt electrospinning (CME) technique, with the goal of optimizing the fabrication parameters to generate fibrous scaffolds for tendon tissue engineering. The effects of CME processing parameters, including rotational speed, voltage, and temperature, on fiber properties (i.e. orientation, mean diameter, and productivity) were systematically investigated. By using this solvent-free and environmentally friendly method, we fabricated both random and aligned poly (L-lactic acid) (PLLA) fibrous scaffolds with controllable mesh thickness. We also investigated and compared their morphology, surface hydrophilicity, and mechanical properties. We seeded human adipose derived mesenchymal stem cells (HADMSC) on various PLLA fibrous scaffolds and conditioned the constructs in tenogenic differentiation medium for up to 21 days, to investigate the effects of fiber alignment and scaffold thickness on cell behavior. Aligned fibrous scaffolds induced cell elongation and orientation through a contact guidance phenomenon and promoted HADMSC proliferation and differentiation towards tenocytes. At the early stage, thinner scaffolds were beneficial for HADMSC proliferation, but the scaffold thickness had no significant effects on cell proliferation for longer-term cell culture. We further co-seeded HADMSC and human umbilical vein endothelial cells (HUVEC) on aligned PLLA fibrous mats and determined how the vascularization affected HADMSC tenogenesis. We found that co-cultured HADMSC-HUVEC expressed more tendon-related markers on the aligned fibrous scaffold. The co-culture systems promoted in vitro HADMSC differentiation towards tenocytes. These aligned fibrous scaffolds fabricated by CME technique could potentially be utilized to repair and regenerate tendon defects and injuries with cell co-culture and controlled vascularization.