How different attributes are weighted in professionals' decision-making in Pediatric Dentistry-a protocol for guiding discrete choice experiment focused on shortening the evidence-based practice implementation for dental care.

BACKGROUND: Important evidence has been constantly produced and needs to be converted into practice. Professional consumption of such evidence may be a barrier to its implementation. Then, effective implementation of evidence-based interventions in clinical practice leans on the understanding of how professionals value attributes when choosing between options for dental care, permitting to guide this implementation process by maximizing strengthens and minimizing barriers related to that. METHODS: This is part of a broader project investigating the potential of incorporating scientific evidence into clinical practice and public policy recommendations and guidelines, identifying strengths and barriers in such an implementation process. The present research protocol comprises a Discrete Choice Experiment (DCE) from the Brazilian oral health professionals' perspective, aiming to assess how different factors are associated with professional decision-making in dental care, including the role of scientific evidence. Different choice sets will be developed, either focusing on understanding the role of scientific evidence in the professional decision-making process or on understanding specific attributes associated with different interventions recently tested in randomized clinical trials and available as newly produced scientific evidence to be used in clinical practice. DISCUSSION: Translating research into practice usually requires time and effort. Shortening this process may be useful for faster incorporation into clinical practice and beneficial to the population. Understanding the context and professionals' decision-making preferences is crucial to designing more effective implementation and/or educational initiatives. Ultimately, we expect to design an efficient implementation strategy that overcomes threats and potential opportunities identified during the DCEs, creating a customized structure for dental professionals. TRIAL REGISTRATION: https://osf.io/bhncv .

Carbonic anhydrase III protects osteocytes from oxidative stress.

Osteocytes are master orchestrators of bone remodeling; they control osteoblast and osteoclast activities both directly via cell-to-cell communication and indirectly via secreted factors, and they are the main postnatal source of sclerostin and RANKL (receptor activator of NF-kB ligand), two regulators of osteoblast and osteoclast function. Despite progress in understanding osteocyte biology and function, much remains to be elucidated. Recently developed osteocytic cell lines-together with new genome editing tools-has allowed a closer look at the biology and molecular makeup of these cells. By using single-cell cloning, we identified genes that are associated with high Sost/sclerostin expression and analyzed their regulation and function. Unbiased transcriptome analysis of high- vs. low-Sost/sclerostin-expressing cells identified known and novel genes. Dmp1 (dentin matrix protein 1), Dkk1 (Dickkopf WNT signaling pathway inhibitor 1), and Phex were among the most up-regulated known genes, whereas Srpx2, Cd200, and carbonic anhydrase III (CAIII) were identified as novel markers of differentiated osteocytes. Aspn, Enpp2, Robo2, Nov, and Serpina3g were among the transcripts that were most significantly suppressed in high-Sost cells. Considering that CAII was recently identified as being regulated by Sost/sclerostin and capable of controlling mineral homeostasis, we focused our attention on CAIII. Here, we report that CAIII is highly expressed in osteocytes, is regulated by parathyroid hormone both in vitro and in vivo, and protects osteocytes from oxidative stress.-Shi, C., Uda, Y., Dedic, C., Azab, E., Sun, N., Hussein, A. I., Petty, C. A., Fulzele, K., Mitterberger-Vogt, M. C., Zwerschke, W., Pereira, R., Wang, K., Divieti Pajevic, P. Carbonic anhydrase III protects osteocytes from oxidative stress.

The new generation of conventional and bulk-fill composites do not reduce the shrinkage stress in endodontically-treated molars.

PURPOSE: To compare flowable and regular paste bulk-fill resin composites with old and new generation conventional composites that use incremental filling techniques for direct restoration of endodontically-treated teeth. METHODS: Four resin composites produced by the same company (3M-ESPE) were used: two conventional resin composites (old formulation, Z100, and new nanofilled formulation, Filtek Supreme XT); and two bulk-fill resin composites (flowable composite, Filtek Bulk-fill Flowable associated with Filtek Supreme, and regular paste, Filtek Bulk-fill Posterior). Elastic modulus (E), Vickers hardness (VH), post-gel shrinkage (Shr), diametral tensile strength (DTS) and compressive strength (CS) were determined (n= 10) and statistically analyzed using ANOVA and Tukey's test (α=0.05). Shrinkage stresses were analyzed using non-linear finite element analysis. RESULTS: Filtek Bulk-fill flowable and Filtek Supreme XT had higher CS than Z100 and Filtek Bulk-fill Posterior. Z100 and Filtek Supreme XT had higher DTS than Filtek Bulk-fill Posterior. Filtek Bulk-fill flowable had the lowest values and Z100 the highest E and Shr. Z100 resulted in higher stresses in the enamel and in root dentin close to the pulp chamber than the other filling techniques. Filtek Bulk-fill Flowable resulted in lower stress than other resin composites. CLINICAL SIGNIFICANCE: Using bulk-fill composites, especially flowable resin composite, created lower stresses in restored endodontically-treated teeth. Clinicians, when deciding for direct restoration of endodontically-treated teeth, may choose the bulk-fill composite to decrease undesirable effects of direct restoration while simplifying filling procedure.

Properties of a Dental Adhesive Containing Graphene and DOPA-Modified Graphene.

Graphene is a promising biomaterial. However, its dispersion in aqueous medium is challenging. This study aimed to modify graphene nanoparticles with L-dopa to improve the properties of experimental dental adhesives. Adhesives were formulated with 0% (control), 0.25%, 0.5%, and 0.75% of graphene, modified or not. Particle modification and dispersion were microscopically assessed. Degree of conversion was tested by Fourier-transform infrared spectroscopy. Flexural strength and modulus of elasticity were evaluated by a 3-point flexural test. Bond strength was tested by shear. To test water sorption/solubility, samples were weighed during hydration and dehydration. Antibacterial activity was tested by Streptococcus mutans colony-forming units quantification. Cytotoxicity on fibroblasts was evaluated through a dentin barrier test. The modification of graphene improved the particle dispersion. Control presented the highest degree of conversion, flexural strength, and bond strength. In degree of conversion, 0.25% of groups were similar to control. In bond strength, groups of graphene modified by L-dopa were similar to Control. The modulus of elasticity was similar between groups. Cytotoxicity and water sorption/solubility decreased as particles increased. Compared to graphene, less graphene modified by L-dopa was needed to promote antibacterial activity. By modifying graphene with L-dopa, the properties of graphene and, therefore, the adhesives incorporated by it were enhanced.

Effect of Different Bleaching Gels Thickeners on Cytotoxicity to Human Gingival Fibroblasts and Enamel Physical Properties: an in Situ Study.

Objective: This study evaluated in vitro, the effects of carbamide peroxide 10% (CP) associated with Carbopol® (CP-ct) and Aristoflex® (CP-at) thickeners on human gingival fibroblasts (HGF) cytotoxicity and assessed in situ their effects on dental enamel. Material and methods: The cytotoxicity was analyzed using MTT - Vybrant® proliferation test. For in situ stage, 144 bovine enamel/dentin blocks were randomized into seven groups (n=12). Samples were stained, fixed in intraoral palatal devices and bleached for 4 h, during 14 days, with: Carbopol thickener (ct), Aristoflex thickener (at), CP-ct, CP-at, CP without thickener (CP-wot), Commercial CP (CP-com). The samples had their microhardness (SMH), roughness (Ra) and color analyzed using a microdurometer, a rugosimeter and a spectrophotometer, respectively. The analyses were performed at baseline and 24-h after completion of tooth bleaching. Results: Different thickeners were similar regarding their cytotoxicity. The experimental gels with Carbopol exhibited lower SMH values, while the groups treated with CP exhibited higher Ra values. For the color change results, the groups treated with CP had values above the acceptability and perceptibility limits. Conclusion: CP-at was able to promote an effective bleaching with less alterations of the tooth surface compared to the CP-ct. Hence, Aristoflex stands as a promising thickener in conjunction with CP in order to preserve the physical properties of dental enamel after home bleaching.

Screening coffee genotypes for brown eye spot resistance in Brazil.

Several researchers have attempted to develop coffee plants that are resistant to brown eye spot (BES); however, no coffee cultivars are resistant to the disease. In the present study, a blend of strains from Cercospora coffeicola was inoculated into 19 Brazilian commercial cultivars and 41 accessions from the Germplasm Collection of Minas Gerais to evaluate the genetic resistance ability within the population and select superior genotypes for the breeding program. After predicting the genotypic values of the estudied material, the evaluations number necessary for selecting genotypes with accuracy and efficiency was determined based on the data of severity to BES. The action of defense mechanisms plant was also investigated by assessing the levels of total soluble phenolic compounds and soluble lignin in contrasting genotypes for disease susceptibility. Based on the results, the accession MG 1207 Sumatra, had an intrinsic genetic capacity to maintain low levels of severity to BES. The genotype MG 1207 Sumatra can substantially contribute to the development of new cultivars, which may lead to the reduced use of pesticides. According to the accuracy and efficiency results obtained, four evaluations BES severity are sufficient to achieve accuracy, providing expressive genetic gains. Finally, the levels of lignin and phenolic compounds were not found to be associated with the resistance of coffee genotypes to BES.

Effect of wetting agent coverage on the surface properties of resin composite submitted to brushing and staining cycles.

BACKGROUND: Wetting agents facilitate the composites handling, acting as a lubricant and decreasing their stickness to spatula. The effects of these materials on the properties of composites are not completely clear. This study aimed to evaluate Gloss, Color, Roughness and Microhardness of a composite (Filtek Z250 XT, 3M Oral Care) covered by a wetting agent (Modeling Resin, Bisco), submitted to brushing and staining cycles with red wine. MATERIAL AND METHODS: Cylinder-shaped samples (8 mm ø x 2 mm height) were divided into 4 groups, according to application of wetting agent and brushing cycles (n = 20). The composite was placed in the orifice of a polytetrafluoroethylene mold, received wetting agent coverage, and was light-cured. Gloss, Color, Roughness and Microhardness were evaluated in two times: after samples confection and after brushing + staining cycles. Data were submitted to one-way ANOVA and Tukey's test (ΔL, Δa, Δb and ΔE) and two-way repeated measures ANOVA and Bonferronis's test (gloss, roughness and microhardness). RESULTS: In general, the composite showed higher gloss values when added by the wetting agent. No statistical differences were observed regarding colors' groups. Roughness increased after brushing cycles, regardless of wetting agent application. The only group which presented decreased Microhardness after cycles was the group without wetting agent, only submitted to staining with red wine. CONCLUSIONS: The application of wetting agent on the composite did not interfere negatively with its properties of Gloss, Color, Roughness and Microhardness. Key words:Resin composite, wetting agent, surface properties, gloss, color, esthetic dentistry.

Effect of handpiece light and material used in pulp chamber on dentin removal during root canal retreatment.

This study evaluated the effect of the use of glass ionomer cement (GIC) and flowable bulk-fill resin composite (BFRC) for filling pulp chambers and the type of high-speed handpiece light used on dentin removal during access preparation for endodontic retreatment in molar teeth. Twenty maxillary molars were treated endodontically. BFRC (Opus Bulk Fill Flow APS, FGM) was used to fill the pulp chamber and replace coronal dentin (n = 10). In the remaining teeth, the pulp chamber was filled with GIC (Maxion R, FGM). Conventional resin composite (Opallis, FGM) was used to restore the enamel layer in all teeth. The samples in each group were divided into two subgroups, and the root canals were reaccessed using a handpiece with white or ultraviolet light. The teeth were scanned using micro-CT before and after root canal reaccess. The dentin volume removed was calculated and analyzed using 2-way analysis of variance and Tukey's test (α = 0.05). The crown and pulp chamber locations with dentin removal are described using frequency distribution. During the access, fewer pulp chamber walls were affected and a lower volume of dentin was removed from the pulpal floor in the group restored with GIC than in the group restored with BFRC. No effect was observed on the coronal dentin walls with respect to the filling protocols and type of light used. For dentin removal from the pulp chamber, handpieces with white light performed better than those with ultraviolet light, irrespective of the filling protocol used. The use of GIC to fill the pulp chamber and use of white handpiece light reduced dentin removal from the pulpal floor and resulted in fewer affected dentin walls.

Direct resin composite restoration of endodontically-treated permanent molars in adolescents: bite force and patient-specific finite element analysis.

Objective To evaluate the influence of three levels of dental structure loss on stress distribution and bite load in root canal-treated young molar teeth that were filled with bulk-fill resin composite, using finite element analysis (FEA) to predict clinical failure. Methodology Three first mandibular molars with extensive caries lesions were selected in teenager patients. The habitual occlusion bite force was measured using gnathodynamometer before and after endodontic/restoration procedures. The recorded bite forces were used as input for patient-specific FEA models, generated from cone-beam computed tomographic (CT) scans of the teeth before and after treatment. Loads were simulated using the contact loading of the antagonist molars selected based on the CT scans and clinical evaluation. Pre and post treatment bite forces (N) in the 3 patients were 30.1/136.6, 34.3/133.4, and 47.9/124.1. Results Bite force increased 260% (from 36.7±11.6 to 131.9±17.8 N) after endodontic and direct restoration. Before endodontic intervention, the stress concentration was located in coronal tooth structure; after rehabilitation, the stresses were located in root dentin, regardless of the level of tooth structure loss. The bite force used on molar teeth after pulp removal during endodontic treatment resulted in high stress concentrations in weakened tooth areas and at the furcation. Conclusion Extensive caries negatively affected the bite force. After pulp removal and endodontic treatment, stress and strain concentrations were higher in the weakened dental structure. Root canal treatment associated with direct resin composite restorative procedure could restore the stress-strain conditions in permanent young molar teeth.

Characterization of low-cost Brazilian resin composites submitted to tooth brushing.

This in vitro study aimed to evaluate surface microhardness, roughness, color, gloss and topography of low-cost Brazilian resin composites, compared with an international one. All 120 samples (8mm ø x 2mm height) were prepared and divided into 5 groups: Ultrafill (Biodinâmica), Llis (FGM), Fill Magic (Coltene), Applic (Makira), and Filtek Z250XT (3M Oral Care). Surface microhardness (KHN) (n=10) was analyzed at two time periods: before and after simulated tooth brushing (STB). Other standardized samples of each group (n=10) were analyzed for surface roughness (Ra), color (ΔL, Δa, Δb, ΔE, ΔE00) and gloss unit (GU) at the same two periods. The topography of non-brushed and brushed samples of each group (n=4) was evaluated by scanning electron microscopy. Data were analyzed statistically by two-way repeated measures ANOVA with Bonferroni post-hoc test (KHN, Ra, GU), and one-way ANOVA with Tukey post-hoc test (ΔL, Δa, Δb, ΔE, ΔE00) (α=0.05). Topographic images were submitted to descriptive analysis. The low-cost Brazilian resin composites investigated were compared with the international one and revealed: lower KHN, regardless of before or after STB; statistically similar Ra before STB, except Ultrafill, which presented higher values; lower Ra after STB, except Ultrafill, which presented statistically similar values; statistically similar color change in ΔL, Δa, Δb parameters; statistically similar color change in ΔE, ΔE00 parameters, except Fill Magic, which presented lower values; lower gloss before STB; lower gloss after STB, except Ultrafill, which presented statistically similar values. In a situation of scarce resources, low-cost Brazilian composites might be an acceptable cost-effective restorative alternative.

Effect of Endodontic Sealer in Young Molars Treated by Undergraduate Students - A Randomized Clinical Trial.

The progression of caries in permanent molar teeth of young patients, frequently result in endodontic treatment. This randomized blinded clinical trial assessed the effect of two endodontic sealers (Sealer 26 and AH Plus) on the incidence of pain and apical repair after endodontic treatment of young molar teeth, and secondly identify factors associated with the outcomes. Endodontic treatment was performed by undergraduate students in molar teeth (n=69) of young patients (n=54) at the Endodontic and Restorative Public Extension Clinic Service. Protaper Next was used and the endodontic sealers were allocated which were restored with direct composite resin. Two primary clinical outcomes - apical repair and postoperative pain, were assessed after 12 and 24 months for blinded operators. Description of incidence rates and mixed-model regression using Generalized Estimation Equations (GEE). After 2 years 69 molar teeth of 54 patients were evaluated. No effect of the endodontic sealer was observed irrespective of period of evaluation. Apical repair incidences and asymptomatic teeth were, respectively, 90.5 and 89.3, 96.8 and 90.0% during 1 and 2 years of follow-up. Failed apical repair was associated with unsatisfactory pulpectomy (p=0.003) and periapical conditions (p=0.007) as well as their interaction (p=0.016). None of these independent variables was able to predict the occurrence of pain in both periods. Prognosis of apical repair is dependent on the initial conditions. Endodontic treatment of young molars associated with composite resin restorations performed by undergraduate students have satisfactory results after 2 years and was effective to prevent the risk of permanent teeth loss.

Practice-based analysis of direct posterior dental restorations performed in a public health service: Retrospective long-term survival in Brazil.

The aim of this retrospective study was to evaluate the survival and associated factors for the longevity of direct posterior restorations and to verify whether the geographic location of public health units could influence the long-term survival of such restorations. Data were extracted from electronic patient files of the Brazilian public oral health services. The sample comprised 2,405 class I and II restorations performed 4 to 24 years ago (mean, 8.9 years) in 351 patients (6.8 teeth/patient) across 12 public health units located in different city regions (42 professionals-55 restorations). The restoration was considered successful if it had not been repaired or replaced at the time of evaluation; failure was defined as replacement of the restoration, the need for endodontic treatment, tooth/restoration fracture or tooth extraction. Data were analyzed using the Kaplan-Meier test for restoration survival and Cox regression to evaluate the factors associated with failure. The majority of the restorations involved the use of amalgam (85%), involved a single face (70%), and were without pulp/dentin capping (85%). The overall survival rate was 95%, and the mean observation time was 8.9 years. The restoration survival was 79% (95% CI: 60.6-89.5) over 24 years, and the mean survival time was 22.2 years (95% CI: 21.9-22.6 years). The annual failure rate up to 24 years was 0.9%. After the adjustment, only the number of restored faces and the geographic location where the restoration was performed remained associated with failure of the restoration. The direct posterior restorations performed at the evaluated public health service units presented high survival rates. The restorations of people with lower access to POHS had lower survival rates. Class I restorations presented higher survival rates than class II restorations with two or more faces, regardless of the restorative material used.

Evaluation of Bond Strength, Nanoleakage, and Marginal Adaptation of Bulk-fill Composites Submitted to Thermomechanical Aging.

PURPOSE: To evaluate the bond strength (BS), nanoleakage, and marginal adaptation (MA) of three bulk-fill and one conventional composite, submitted or not to mechanical and thermal cycling. MATERIALS AND METHODS: Ninety-six molars were selected and 4-mm-deep class I cavities were prepared and restored. Half of the teeth were submitted to mechanical and thermal cycling (MTC). Teeth were divided into 8 groups (n = 12), according to the composite used - Filtek Z350 XT (Z350), Tetric N-Ceram Bulk Fill (TET), Filtek Bulk Fill Posterior Restorative (FBF) and SonicFill (SF) - and aging, submitted or not to MTC. Fifty-six teeth (n = 7) were used for bond-strength testing, which was performed on stick-shaped specimens obtained from the restored area. Two sticks per tooth were selected to assess nanoleakage. For MA analysis, 40 teeth (n = 5) were sectioned parallel and perpendicular to the occlusal surface and resin-based replicas from the obtained surfaces were prepared. Fracture pattern, nanoleakage, and MA were evaluated using SEM. Quantitative analysis of nanoleakage and MA were performed with ImageJ software. Data obtained were submitted to two-way ANOVA and Tukey's test (ɑ = 0.05). RESULTS: TET presented good MA and higher values of BS when compared to SF. Z350 and FBF presented poorer MA and lower BS, which was statically similar to the other groups. SF obtained the best MA values. Regarding nanoleakage, the highest values were obtained for TET, which differed significantly from the other groups, which presented similar results among themselves. Aging by MTC solely affected MA. CONCLUSION: Bulk-fill composites presented similar performance to the conventional nanocomposite and remained stable when aged.

Effect of cavity preparation design and ceramic type on the stress distribution, strain and fracture resistance of CAD/CAM onlays in molars.

Objective This study aimed to evaluate the effect of the cavity preparation and ceramic type on the stress distribution, tooth strain, fracture resistance and fracture mode of human molar teeth restored with onlays. Material and Methods Forty-eight molars were divided into four groups (n=12) with assorted combinations of two study factors: BL- conventional onlay preparation with boxes made from leucite ceramic (IPS-Empress CAD, Ivoclar Vivadent); NBL- conservative onlay preparation without boxes made from leucite ceramic; BD- conventional onlay preparation with boxes made from lithium disilicate glass ceramic (IPS e.max CAD, Ivoclar Vivadent); NBL- conservative onlay preparation with boxes made from lithium disilicate glass ceramic cuspal deformation (µS) was measured at 100 N and at maximum fracture load using strain gauge. Fracture resistance (N) was measured using a compression test, and the fracture mode was recorded. Finite element analysis was used to evaluate the stress distribution by modified von Mises stress criteria. The tooth strain and fracture resistance data were analyzed using the Tukey test and two-way ANOVA, and the fracture mode was analyzed by the chi-square test (α=0.05). Results The leucite ceramic resulted in higher tooth deformation at 100 N and lower tooth deformation at the maximum fracture load than the lithium disilicate ceramic (P<0.001). The lithium disilicate ceramic exhibited higher fracture resistance than the leucite ceramic (P<0.001). The conservative onlay resulted in higher fracture strength for lithium disilicate ceramic. Finite element analysis results showed the conventional cavity preparation resulted in higher stress concentration in the ceramic restoration and remaining tooth than the conservative onlay preparation. The conservative onlays exhibited increased fracture resistance, reduced stress concentration and more favorable fracture modes. Conclusion Molars restored with lithium disilicate CAD-CAM ceramic onlays exhibited higher fracture resistance than molars restored with leucite CAD-CAM ceramic onlays.

Irradiance and Radiant Exposures Delivered by LED Light-Curing Units Used by a Left and Right-Handed Operator.

The combination of the restoration location, the hand preference of the operator using the light-curing unit (LCU), and the design of the LCU all can have an impact on the amount of the light delivered to the restoration. To evaluate the effect of left-handed or right-handed users, the position of the operator (dentist or assistant), and the LCU design on the irradiance, radiant exposure and emission spectrum delivered to the same posterior tooth. Two light emitting diode (LED) LCUs were tested: an angulated monowave LCU Radii-Cal (SDI, Victoria, Australia) and a straight aligned multi-peak LCU Valo Cordless (Ultradent, South Jordan, UT, USA). The irradiance values (mW/cm2), radiant exposure (J/cm2) and emission spectrum were measured using a sensor in maxillary left second molar tooth. The irradiance and radiant exposure were analyzed using three-way ANOVA followed by Tukey test (a=0.05). The emission spectra (nm) were analyzed descriptively. The interaction between LCU design, operator position, and hand preference significantly influenced the irradiance and radiant exposure (P<0.001). In all cases, Valo delivered significantly higher irradiance than Radii-Cal. The handedness and the operator position affected the irradiance and radiant exposure delivered from Valo. Operator position and access affect the irradiance and radiant exposure delivered to the maxillary left second molar. The irradiance and radiant exposure can be greater when a right-hand operator is positioned on the right side of the chair and a left-hand operator is positioned on the left side of the chair. This may result in better resin composite polymerization.

Physical and photoelastic properties of bulk-fill and conventional composites.

PURPOSE: This study evaluated the influence of thickness increment on degree of conversion (DC), Knoop microhardness (KHN), and polymerization-shrinkage stress (PSS) by photoelasticity of three dental composites. METHODS: For DC and KHN, 45 samples were prepared and divided into nine groups (n=5), according to composite (microhybrid [Filtek Z250 - Z250], bulk-fill flowable [SureFil SDR Flow - SDR], and nanohybrid composite [N'Durance - NDU]) and increment thickness (1, 1.5, and 3 mm). PSS was measured by photoelastic analysis. Composites were placed into a photo-elastic model cavity and light-cured. DC and KHN data were subjected to two-way ANOVA and Bonferroni post hoc test. PSS results were qualitatively evaluated through Kruskal-Wallis test. RESULTS: SDR showed the highest DC values. At top and bottom surfaces, the highest KHN was obtained by Z250. Z250 showed higher PSS than SDR in 1.5 mm increments. NDU showed higher PSS than SDR in 3 mm increments. CONCLUSION: The bulk-fill composite demonstrated better DC and similar KHN and PSS in deeper layers compared to conventional composites. Bulk-fill composites may perform as well as conventional nanohybrid and microhybrid composites.

Consumo de processados e ultraprocessados por gestantes da atenção básica no tocantins / Consumption of processed and ultraprocessed by pregnant pregnant women in basic care in Tocantins / Consumo de procesados y ultraprocesados por mujeres embarazadas en cuidados básicos en Tocantins

Introdução:oconsumo frequente de alimentos industrializados, prontos para o consumo, é considerado um mau hábito alimentar em todas as fases do ciclo da vida e pode ser especialmente prejudicial aos grupos populacionais mais vulneráveis, como as mulheres grávidas.Noentanto, são preparações altamente palatáveis e práticas que, mesmo contraindicadas, continuam representando parcela importante no cardápio dos brasileiros.Objetivo:caracterizar o consumo de alimentos quanto ao grau de processamento, por gestantes adultas, atendidas na atenção básica de Palmas, Tocantins.Metodologia:o estudo investigou 60 mulheres em quatro dos sete territórios de saúde de Palmas, por meio de um recordatórioque investigou o consumo alimentar nas 24 horas anteriores à entrevista. Foram estimados pelo Dietbox, o consumo de energia, macronutrientes, ferro, cobalamina e ácido fólico totais e por grupo de alimentos, conformecategorização em quatro grupos:Grupo 1: alimentos in natura ou minimamente processados; Grupo 2: Ingredientes culinários processados; Grupo 3: Alimentos processados; Grupo 4: Alimentos ultraprocessados. Os dados foram analisados no programa Statistical Package for Social Sciences versão 23.0,por meio de estatísticas descritivas.Resultados:observou-se para o grupo de gestantes estudadas que a ingestão de micronutrientes, em sua maior parte, foi oriunda de alimentos in natura ou minimamente processados, reforçando a importância da ingestão desses tipos de alimentos para uma dieta saudável, sobretudo na gestação.No entanto, observou-se que os alimentos processados e ultraprocessados(vegetais em conservas, alguns pães, macarrão instantâneo, bolacha recheada, margarina, achocolatados, refrigerantese salsichas)ainda figuram expressivamente nas dietas das gestantes estudadas, tendo fornecido em média 28% da energia diária consumida, 83,2% dos carboidratos diários, 22% dos lipídeos totais e 15,25% das proteínas.Conclusão:processados e ultraprocessados ainda figuram na dieta das gestantes, fornecendo percentuais expressivos da energia e dos carboidratos ingeridos, porém não ofertando quantidades razoáveis de ferro, cobalamina ou ácido fólico (AU).

Introduction:the frequent consumption of industrialized, ready-to-eat foods is considereda bad eating habit at all stages of the life cycle and can be especially harmful to the most vulnerable population groups, such as pregnant women. However, they are highly palatable and practical preparations that, even contraindicated, continue to represent an important part of the Brazilian menu. Objective: to characterize food consumption according to the degree of processing, by adult pregnant women attended in primary care in Palmas, Tocantins.Methodology: the study investigated 60 women in four of the seven health territories in Palmas, through a recall that investigated food consumption in the 24 hours prior to the interview. The consumption of energy, macronutrients, iron, cobalamin and folic acid total and by food group were estimated by Dietbox, as categorized into four groups: Group 1: fresh or minimally processed foods; Group 2: Processed culinary ingredients; Group 3: Processed foods; Group 4: Ultra-processed foods. Data were analyzed using the Statistical Package for Social Sciences version 23.0 using descriptive statistics. Results:it was observed for the group of pregnant women studied that the intake of micronutrients, for the most part, came from fresh or minimally processed foods, reinforcing the importance of ingesting these types of foods for a healthy diet, especially during pregnancy. However, it was observed that processed and ultra-processed foods (canned vegetables, some breads, instant noodles, stuffed biscuits, margarine, chocolate drinks, soft drinks and sausages) still figure significantly in the diets of the pregnant women studied, providing an average of 28% of the daily energy consumed, 83.2% of daily carbohydrates, 22% of total lipids and 15.25% of proteins. Conclusion: processed and ultra-processed products still figure in the pregnant women's diet, providing significant percentages of energy and carbohydrates ingested, but not offering reasonable amounts of iron, cobalamin or folic acid (AU).

Introducción:elconsumo de alimentos industrializados se considera un mal hábito alimentario en todas las etapas del ciclo de vida,puede ser especialmente perjudicial para los grupos de población más vulnerables, como las mujeres embarazadas. Sin embargo, son preparaciones muy apetecibles y prácticas que, incluso contraindicadas, continúan representando una parte importante del menú brasileño.Objetivo: caracterizar el consumo de alimentos según el grado de procesamiento, por gestantes adultas atendidas en atención primaria en Palmas, Tocantins.Metodología: el estudio investigó 60 mujeres en cuatro de los siete territorios de salud en Palmas, mediante recordatorio del consumo de alimentos en las 24 horas previas a la entrevista. Dietbox estimó el consumo de energía, macronutrientes, hierro, cobalamina y ácido fólico total por grupo de alimentos, categorizados en cuatro grupos: Grupo 1: alimentos frescos o mínimamente procesados; Grupo 2: Ingredientes culinarios procesados; Grupo 3: Alimentos procesados; Grupo 4: Alimentos ultraprocesados. Los datos se analizaron utilizando el paquete estadístico Statistical Package for Social Sciences version 23.0 y estadística descriptiva.Resultados:para el grupo de gestantes estudiadasla ingesta de micronutrientes, en su mayor parte, provino de alimentos frescos o mínimamente procesados, lo que refuerza la importancia de ingerir este tipo de alimentos para una dieta saludable, especialmente durante el embarazo. Sin embargo, se observó que los alimentos procesados y ultraprocesados (vegetales enlatados, panes, fideos instantáneos, bizcochos rellenos, margarina, bebidas de chocolate, refrescos y embutidos) todavía figuran de manera significativa en las dietas de las embarazadas estudiadas, proporcionando un promedio del28% de la energía diaria, el 83,2% de los carbohidratos diarios, el 22% de los lípidos totales y el 15,25% de las proteínas.Conclusión: los productos procesados y ultraprocesados siguen figurando en la dieta de las embarazadas, aportando porcentajes importantes de energía y carbohidratos, pero sin ofrecer cantidades razonables de hierro, cobalamina o ácido fólico (AU).

Ingestão de nutrientes por gestantes da atenção básica no Tocantins / Nutrient intake by primary care pregnant women in Tocantins / Consumo de nutrientes por mujeres embarazadas de cuidado primario en Tocantins

Introdução:Em decorrência de alterações no comportamento alimentar materno durante os trimestres gestacionais, além das variações diárias e sazonais, podem ocorrer alterações na ingestão de nutrientes, que impactam diretamente no resultado obstétrico, sobretudo durante o primeiro trimestre de gestação. Objetivo:investigar o consumo de nutrientes por gestantes adultas, assistidas na atenção básica de Palmas,Tocantins, buscando identificar precocemente desvios evitáveis por meio de orientações dietéticas pré-natais.Metodologia:oestudo investigou 50 mulheres em quatro dos sete territórios de saúde de Palmas,Tocantins,por meio de umrecordatório que investigou o consumo alimentar nas 24 horas anteriores à entrevista. Foram estimadospelo Dietbox, a ingestão diária de energia, carboidratos, proteínas, lipídios totais, ferro, calciferol, folato e cobalamina sem considerar os nutrientes obtidos por meio da suplementação medicamentos.Os dados foram analisados no programa Statistical Package for Social Sciences versão 23.0por meio de estatísticas descritivas.Resultados:foram observados altos percentuais de inadequação da ingestão para todos os macros e micronutrientes estudados na população de gestantes. Destacaram-se 82% das gestantes com ingestões inadequadas de energia; 98% de inadequação para o ácido fólico e 94% para ferro e cobalamina. A ingestão diária de calciferol esteve inadequada em 100% das gestantes. Conclusão: ogrupo de gestantes estudado está ingerindo nutrientes aquém do recomendado, estando o binômio mãe-filho sujeitos a resultados obstétricos indesejáveis, caso nenhuma intervenção seja realizada (AU).

Introduction: Due to changes in maternal eating behavior during the gestational trimesters, in addition to daily and seasonal variations, changes in nutrient intake may occur, which directly impact obstetric outcome, especially during the first trimester of pregnancy.Objective: to investigate the consumption of nutrients by adult pregnant women, assisted in primary care in Palmas, Tocantins, seeking to early identify preventable deviations through prenatal dietary guidelines.Methodology: the study investigated 50 womenin four of the seven health territories in Palmas, through a recall that investigated food consumption in the 24 hours prior to the interview. The daily intake of energy, carbohydrates, proteins, total lipids, iron, calciferol, folate and cobalamin were estimated by Dietbox without considering the nutrients obtained through drug supplementation. Data were analyzed using the Statistical Package for Social Sciences version 23.0using descriptive statistics.Results: high percentages of inadequate intake wereobserved for all macro and micronutrients studied in the population of pregnant women. 82% of pregnant women with inadequate energy intake stood out; 98% unsuitable for folic acid and 94% for iron and cobalamin. The daily intake of calciferol was inadequate in 100% of the pregnant women.Conclusion: the group of pregnant women studied is ingesting less than recommended nutrients, and the mother-child binomial is subject to undesirable obstetric results, if no intervention is performed (AU).

Introducción: Debido a los cambios en la conducta alimentaria materna durante los trimestres gestacionales, además de las variaciones diarias y estacionales, pueden ocurrir cambios en la ingesta de nutrientes que impactan directamente en el resultado obstétrico, especialmente durante el primer trimestre del embarazo. Objetivo:investigar el consumo de nutrientes por parte de gestantes adultas, atendidas en atención primaria en Palmas, Tocantins, buscando identificar desviaciones precoces evitables a través de guías dietéticas prenatales. Metodología:el estudio investigó a 50 mujeres en cuatro de los siete territorios de salud en Palmas, a través de un recordatorio que investigó el consumo de alimentos en las 24 horas previas a la entrevista. Dietbox estimó la ingesta diaria de energía, carbohidratos, proteínas, lípidos totales, hierro, calciferol, folato y cobalamina sin considerar los nutrientes obtenidos a través de la suplementación farmacológica. Los datos se analizaron utilizando el paquete estadístico para ciencias sociales versión 23.0utilizando estadística descriptiva. Resultados:se observaron altos porcentajes de ingesta inadecuada para todos los macro y micronutrientes estudiados en la población de gestantes. Se destacó el 82% de las embarazadas con aporte energético inadecuado; 98% inadecuado para ácido fólico y 94% para hierro y cobalamina. La ingesta diaria de calciferol fue inadecuada en el 100% de las embarazadas. Conclusión: el grupo de gestantes estudiado está ingiriendo menos nutrientes de los recomendados, y el binomio madre-hijo está sujeto a resultados obstétricos indeseables, si no se realiza ninguna intervención (AU).

Osteocyte Protein Expression Is Altered in Low-Turnover Osteoporosis Caused by Mutations in WNT1 and PLS3.

Context: Osteocytes express proteins that regulate bone remodeling and mineralization. Objective: To evaluate the relationship between osteocyte-specific protein expression and bone histology in patients with monogenic osteoporosis due to wingless integration site 1 (WNT1) or plastin 3 (PLS3) mutations. Design and Setting: Cross-sectional cohort study at a university hospital. Participants: Six patients (four males; ages: 14 to 72 years) with a heterozygous WNT1 mutation and five patients (four males; ages: 9 to 70 years) with a heterozygous/hemizygous PLS3 mutation. Methods and Main Outcome Measures: Immunohistochemistry was performed for fibroblast growth factor 23 (FGF23), dentin matrix protein 1 (DMP1), sclerostin, and phosphorylated (phospho-)ß-catenin in iliac crest samples and compared with bone histomorphometry. Results: FGF23 expression in WNT1 patients was 243% that observed in PLS3 patients (P < 0.01). DMP1, sclerostin, and phospho-ß-catenin expression did not differ between groups. Serum phosphate correlated inversely with FGF23 expression (r = -0.79, P = 0.01) and serum ionized calcium correlated inversely with sclerostin expression (r = -0.60, P = 0.05). Phospho-ß-catenin expression correlated inversely with DMP1 expression (r = -0.88, P < 0.001), osteoid volume/bone volume (r = -0.68, P = 0.02), and bone formation rate (r = -0.78, P < 0.01). FGF23 expression did not correlate with DMP1 expression, sclerostin expression, or bone histomorphometry. Marrow adiposity was higher in WNT1 than in PLS3 patients (P = 0.04). Conclusions: Mutations that disrupt WNT signaling and osteocytic mechanosensing affect osteocyte protein expression. Abnormal osteocyte function may play a role in the pathogenesis of monogenetic forms of osteoporosis.

Effect of handpiece light and material used in pulp chamber on dentin removal during root canal retreatment

Abstract This study evaluated the effect of the use of glass ionomer cement (GIC) and flowable bulk-fill resin composite (BFRC) for filling pulp chambers and the type of high-speed handpiece light used on dentin removal during access preparation for endodontic retreatment in molar teeth. Twenty maxillary molars were treated endodontically. BFRC (Opus Bulk Fill Flow APS, FGM) was used to fill the pulp chamber and replace coronal dentin (n = 10). In the remaining teeth, the pulp chamber was filled with GIC (Maxion R, FGM). Conventional resin composite (Opallis, FGM) was used to restore the enamel layer in all teeth. The samples in each group were divided into two subgroups, and the root canals were reaccessed using a handpiece with white or ultraviolet light. The teeth were scanned using micro-CT before and after root canal reaccess. The dentin volume removed was calculated and analyzed using 2-way analysis of variance and Tukey's test (α = 0.05). The crown and pulp chamber locations with dentin removal are described using frequency distribution. During the access, fewer pulp chamber walls were affected and a lower volume of dentin was removed from the pulpal floor in the group restored with GIC than in the group restored with BFRC. No effect was observed on the coronal dentin walls with respect to the filling protocols and type of light used. For dentin removal from the pulp chamber, handpieces with white light performed better than those with ultraviolet light, irrespective of the filling protocol used. The use of GIC to fill the pulp chamber and use of white handpiece light reduced dentin removal from the pulpal floor and resulted in fewer affected dentin walls.

Resumo Este estudo avaliou os efeitos do cimento de ionômero de vidro (GIC) e da resina composta fluida bulk fill (BFRC) usados como preenchimento da câmara pulpar; e o tipo de iluminação das turbinas de alta rotação na remoção dentinária após cavidades de acesso para retratamento endodôntico em dentes molares. Vinte molares superiores foram tratados endodonticamente. Dez dentes foram restaurados usando BFRC (Opus Bulk Fill Flow APS, FGM) para preencher a câmara pulpar e dentina coronária; e resina composta convencional (Opallis, FGM) para restaurar a camada de esmalte. Os outros dentes foram restaurados usando GIC (Maxion R, FGM) para preencher a câmara pulpar e resina composta (Opallis, FGM). As amostras foram divididas em dois grupos e os canais radiculares foram novamente acessados com turbina de alta-rotação com iluminação branca ou ultravioleta. Os dentes foram escaneados usando micro-CT antes e após o novo acesso ao canal radicular. O volume de dentina removida foi calculado e os dados foram analisados por ANOVA bidirecional e teste de Tukey (α=0,05). As regiões na coroa e na câmara pulpar que apresentaram dentina removida no acesso dos canais foram descritas por meio de distribuição por frequência. A reabertura do canal radicular com GIC resultou em menos paredes afetadas da câmara pulpar e menor volume de dentina removida no assoalho. Nenhum efeito foi observado nas paredes de dentina coronária considerando aos protocolos de preenchimento. A turbina de alta rotação com iluminação branca reduziu a remoção de dentina da câmara pulpar, independentemente do protocolo de restauração utilizado. O uso de turbina de alta rotação com iluminação branca e GIC para preencher a câmara pulpar reduziram a remoção de dentina do assoalho e afetaram menos paredes dentinárias.