RESUMO
We present the preparation of 11 nm polyacrylamide-stabilized polystyrene latex particles for conjugation to a microRNA model by surfactant-free RAFT emulsion polymerization. Our synthetic strategy involved the preparation of amphiphilic polyacrylamide-block-polystyrene copolymers, which were able to self-assemble into polymeric micelles and "grow" into polystyrene latex particles. The surface of these sterically stabilized particles was postmodified with a disulfide-bearing linker for the attachment of the microRNA model, which can be released from the latex particles under reducing conditions. These nanoparticles offer the advantage of ease of preparation via a scaleable process, and the versatility of their synthesis makes them adaptable to a range of applications.
Assuntos
Portadores de Fármacos/síntese química , Látex/química , MicroRNAs/administração & dosagem , Nanopartículas/química , Poliestirenos/química , Resinas Acrílicas/química , Portadores de Fármacos/química , Liberação Controlada de Fármacos , Emulsões/química , Oxirredução , Polimerização , Tensoativos/químicaRESUMO
The authors report the preparation of a novel range of functional polyacrylamide stabilized polystyrene nanoparticles, obtained by surfactant-free reversible addition-fragmentation chain transfer (RAFT) emulsion polymerization, their fluorescent tagging, cellular uptake, and biodistribution. The authors show the versatility of the RAFT emulsion process for the design of functional nanoparticles of well-defined size that can be used as drug delivery vectors. Functionalization with a fluorescent tag offers a useful visualization tool for tracing, localization, and clearance studies of these carriers in biological models. The studies are carried out by labeling the sterically stabilized latex particles chemically with rhodamine B. The fluorescent particles are incubated in a healthy human renal proximal tubular cell line model, and intravenously injected into a mouse model. Cellular localization and biodistribution of these particles on the biological models are explored.
Assuntos
Portadores de Fármacos , Corantes Fluorescentes/química , Nanopartículas/química , Imagem Óptica/métodos , Rodaminas/química , Coloração e Rotulagem/métodos , Resinas Acrílicas/química , Animais , Transporte Biológico , Linhagem Celular , Emulsões , Células Epiteliais/citologia , Células Epiteliais/metabolismo , Humanos , Injeções Intravenosas , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Microesferas , Nanopartículas/administração & dosagem , Tamanho da Partícula , Polimerização , Poliestirenos/química , Distribuição TecidualRESUMO
Alternating-block hyperbranched polymers were synthesized using the highly versatile thiol-yne reaction. Dimethyl acrylamide-styrene and tert-butyl acrylate-styrene polymers were prepared, with subsequent hydrolysis of the tert-butyl ester to acrylic acid. The dimethyl acrylamide-styrene hyperbranched polymers self-assembled into large aggregates, as did the acrylic acid-styrene system at low pH. However, high pH triggers the formation of very well defined small particles in the latter system.