[Drug delivery of CPC/amifostine/cisplatin complex in vitro and its ability in repairing bone defect and eliminating tumor in vivo].

OBJECTIVE: To investigate the feasibility of using calcium phosphate cement/amifostine/cisplatin complex to fill and repair bone defect, caused by tumor resection. METHODS: Drug concentration in the CPC/ amifostine/cisplatin complex was determined. Rabbits with bone defect and rats with osteosarcoma were implanted with CPC and CPC/amifostine/cisplatin complex. RESULTS: Similar bone growth was observed in the femurs of rabbits implanted with CPC/amifostine/cisplatin complex and those implanted with CPC. CPC/amifostine/cisplatin complex delivered amifostine and cisplatin consistently and eliminated osteosarcoma cells implanted in the rats. CONCLUSION: CPC/amifostine/cisplatin complex repairs bone defect caused by tumors as a filling material.

[Physicochemical and sustained drug release properties of calcium phosphate cement/amifostine/cisplatin complex and its effect in repairing bone defect due to tumor resection in rabbits].

OBJECTIVE: To investigate the feasibility of using calcium phosphate cement/amifostine complex as an new filling material for repairing bone defect caused by tumor resection. METHODS: Calcium phosphate cement (CPC)/cisplatin/amifostine complex was prepared at the mass ratio of 1000:2:5. The setting time, mechanical strength, and porosity of the complex were determined, and scanning electron microscopy and assessment of sustained drug release and inhibitory effect against osteosarcoma cells were carried out. The degradation of the material and new bone ingrowth were also observed in a rabbit model of femoral bone defect. RESULTS: The setting time, strength, and porosity, appearances under scanning electron microscope, and sustained drug release properties of CPC/cisplatin/amifostine complex were identical to those of CPC, and the integration of amifostine in the complex did not affect the cytotoxicity of cisplatin against the osteosarcoma cells. Pathological evidences of the degradation of the material and new bone ingrowth into the material were observed with the passage of time following its implantation into the bone defect in rabbits. CONCLUSION: The CPC/cisplatin/amifostine complex can be used as a filling material for repairing bone defect caused by tumor resection and eliminating the residual tumor cells in rabbits.