RESUMO
Microscale hydrogels can be synthesized within microfluidic systems and subsequently assembled to make tissues composed of units such as myofibers in muscle tissue. Importantly, the nanofibrous surface of hydrogels is essential for tissue engineering aims due to inducing beneficial cell-surface interactions. In this study, a new microfluidic platform, embedded with a hydrogel, was introduced that allowed for performing multiple non-parallel steps for the synthetic approaches. Satellite cells, isolated from skeletal tissues of 10-day Naval Medical Research Institute-murine were cultured on the prepared hydrogel within the microfluidic system. The normal proliferation of satellite cells occurred after the employment of continuous perfusion cell culture. Interestingly, the positive results of the immuno-staining assay along with the cellular bridge formation between hydrogel fragments confirmed the muscle differentiation of seeded satellite cells. Further on, COMSOl simulations anticipated that the thermodynamic conditions of the microfluidic system during hydrogel synthesis had to be kept steady while a shear stress value of 15 × 10-6 Pa was calculated, exhibiting a cell culture condition free of environmental stress.
Assuntos
Compostos de Anilina/química , Hidrogéis/química , Desenvolvimento Muscular , Nanofibras/química , Células Satélites de Músculo Esquelético/citologia , Alicerces Teciduais/química , Animais , Materiais Biocompatíveis/química , Técnicas de Cultura de Células/instrumentação , Diferenciação Celular , Células Cultivadas , Dispositivos Lab-On-A-Chip , Camundongos , Nanofibras/ultraestruturaRESUMO
Nanoscale patterning of scaffolds provides broad surface for adhesion and differentiation of stem cells. As we know, the combination of tissue engineering with stem cells technology hold the key for regeneration of damaged tissues for example skeletal muscle tissues. On the other hand, the mechanical assessments of poly ε-caprolacton nanofibers determined the required features of biomedical scaffold for skeletal muscle tissue. In this study, skeletal muscle satellite cells as the main group of stem cells were cultivated on the electrospun poly ε-caprolacton nanofibers. Our results indicated that in comparison with tissue culture polystyrene, the nanoscale of scaffolds provided more induction to matured cells of skeletal muscle. Moreover, the immobilization of cells by collagen on poly ε-caprolacton nanofibers significantly improved the differential potency of satellite cells.