RESUMO
The evolutionary relationships of extinct species are ascertained primarily through the analysis of morphological characters. Character inter-dependencies can have a substantial effect on evolutionary interpretations, but the developmental underpinnings of character inter-dependence remain obscure because experiments frequently do not provide detailed resolution of morphological characters. Here we show experimentally and computationally how gradual modification of development differentially affects characters in the mouse dentition. We found that intermediate phenotypes could be produced by gradually adding ectodysplasin A (EDA) protein in culture to tooth explants carrying a null mutation in the tooth-patterning gene Eda. By identifying development-based character inter-dependencies, we show how to predict morphological patterns of teeth among mammalian species. Finally, in vivo inhibition of sonic hedgehog signalling in Eda null teeth enabled us to reproduce characters deep in the rodent ancestry. Taken together, evolutionarily informative transitions can be experimentally reproduced, thereby providing development-based expectations for character-state transitions used in evolutionary studies.
Assuntos
Evolução Biológica , Fósseis , Dente/anatomia & histologia , Dente/crescimento & desenvolvimento , Animais , Simulação por Computador , Ectodisplasinas/deficiência , Ectodisplasinas/genética , Ectodisplasinas/farmacologia , Feminino , Deleção de Genes , Proteínas Hedgehog/antagonistas & inibidores , Proteínas Hedgehog/genética , Técnicas In Vitro , Masculino , Camundongos , Dente Molar/anatomia & histologia , Dente Molar/efeitos dos fármacos , Dente Molar/crescimento & desenvolvimento , Fenótipo , Transdução de Sinais/efeitos dos fármacos , Dente/efeitos dos fármacosRESUMO
Much of the basic information about individual organ development comes from studies using model species. Whereas conservation of gene regulatory networks across higher taxa supports generalizations made from a limited number of species, generality of mechanistic inferences remains to be tested in tissue culture systems. Here, using mammalian tooth explants cultured in isolation, we investigate self-regulation of patterning by comparing developing molars of the mouse, the model species of mammalian research, and the bank vole. A distinct patterning difference between the vole and the mouse molars is the alternate cusp offset present in the vole. Analyses of both species using 3D reconstructions of developing molars and jaws, computational modeling of cusp patterning, and tooth explants cultured with small braces show that correct cusp offset requires constraints on the lateral expansion of the developing tooth. Vole molars cultured without the braces lose their cusp offset, and mouse molars cultured with the braces develop a cusp offset. Our results suggest that cusp offset, which changes frequently in mammalian evolution, is more dependent on the 3D support of the developing jaw than other aspects of tooth shape. This jaw-tooth integration of a specific aspect of the tooth phenotype indicates that organs may outsource specific aspects of their morphology to be regulated by adjacent body parts or organs. Comparative studies of morphologically different species are needed to infer the principles of organogenesis.
Assuntos
Evolução Biológica , Arcada Osseodentária , Desenvolvimento Maxilofacial/fisiologia , Dente/anatomia & histologia , Animais , Arvicolinae/embriologia , Fenômenos Biomecânicos , Simulação por Computador , Embrião de Mamíferos , Desenvolvimento Embrionário , Camundongos , Modelos BiológicosRESUMO
From gastrulation to late organogenesis animal development involves many genetic and bio-mechanical interactions between epithelial and mesenchymal tissues. Ectodermal organs, such as hairs, feathers and teeth are well studied examples of organs whose development is based on epithelial-mesenchymal interactions. These develop from a similar primordium through an epithelial folding and its interaction with the mesenchyme. Despite extensive knowledge on the molecular pathways involved, little is known about the role of bio-mechanical processes in the morphogenesis of these organs. We propose a simple computational model for the biomechanics of one such organ, the tooth, and contrast its predictions against cell-tracking experiments, mechanical relaxation experiments and the observed tooth shape changes over developmental time. We found that two biomechanical processes, differential tissue growth and differential cell adhesion, were enough, in the model, for the development of the 3D morphology of the early tooth germ. This was largely determined by the length and direction of growth of the cervical loops, lateral folds of the enamel epithelium. The formation of these cervical loops was found to require accelerated epithelial growth relative to other tissues and their direction of growth depended on specific differential adhesion between the three tooth tissues. These two processes and geometrical constraints in early tooth bud also explained the shape asymmetry between the lateral cervical loops and those forming in the anterior and posterior of the tooth. By performing mechanical perturbations ex vivo and in silico we inferred the distribution and direction of tensile stresses in the mesenchyme that restricted cervical loop lateral growth and forced them to grow downwards. Overall our study suggests detailed quantitative explanations for how bio-mechanical processes lead to specific morphological 3D changes over developmental time.
Assuntos
Adesão Celular , Odontogênese , Dente/embriologia , Animais , Movimento Celular , Proliferação de Células , Simulação por Computador , Dentina/embriologia , Ectoderma/embriologia , Células Epiteliais/citologia , Gastrulação , Regulação da Expressão Gênica no Desenvolvimento , Técnicas In Vitro , Mesoderma/embriologia , Camundongos , Modelos Biológicos , Transdução de Sinais , Estresse MecânicoRESUMO
It is not known whether natural selection can encounter any given phenotype that can be produced by genetic variation. There has been a long-lasting debate about the processes that limit adaptation and, consequently, about how well adapted phenotypes are. Here we examine how development may affect adaptation, by decomposing the genotype-fitness map-the association between each genotype and its fitness-into two: one mapping genotype to phenotype by means of a computational model of organ development, and one mapping phenotype to fitness. In the map of phenotype and fitness, the fitness of each individual is based on the similarity between realized morphology and optimal morphology. We use three different simulations to map phenotype to fitness, and these differ in the way in which similarity is calculated: similarity is calculated for each trait (in terms of each cell position individually), for a large or a small number of phenotypic landmarks (the 'many-traits' and 'few-traits' phenotype-fitness maps), and by measuring the overall surface roughness of morphology (the 'roughness' phenotype-fitness map). Evolution is simulated by applying the genotype-phenotype map and one phenotype-fitness map to each individual in the population, as well as random mutation and drift. We show that the complexity of the genotype-phenotype map prevents substantial adaptation in some of the phenotype-fitness maps: sustained adaptation is only possible using 'roughness' or 'few-traits' phenotype-fitness maps. The results contribute developmental understanding to the long-standing question of which aspects of phenotype can be effectively optimized by natural selection.
Assuntos
Adaptação Fisiológica/genética , Genótipo , Modelos Biológicos , Fenótipo , Dente/crescimento & desenvolvimento , Algoritmos , Evolução Biológica , Simulação por Computador , Aptidão Genética/genética , Densidade Demográfica , Análise de Componente Principal , Seleção Genética , Dente/anatomia & histologiaRESUMO
The relationship between the genotype and the phenotype, or the genotype-phenotype map, is generally approached with the tools of multivariate quantitative genetics and morphometrics. Whereas studies of development and mathematical models of development may offer new insights into the genotype-phenotype map, the challenge is to make them useful at the level of microevolution. Here we report a computational model of mammalian tooth development that combines parameters of genetic and cellular interactions to produce a three-dimensional tooth from a simple tooth primordia. We systematically tinkered with each of the model parameters to generate phenotypic variation and used geometric morphometric analyses to identify, or developmentally ordinate, parameters best explaining population-level variation of real teeth. To model the full range of developmentally possible morphologies, we used a population sample of ringed seals (Phoca hispida ladogensis). Seal dentitions show a high degree of variation, typically linked to the lack of exact occlusion. Our model suggests that despite the complexity of development and teeth, there may be a simple basis for dental variation. Changes in single parameters regulating signalling during cusp development may explain shape variation among individuals, whereas a parameter regulating epithelial growth may explain serial, tooth-to-tooth variation along the jaw. Our study provides a step towards integrating the genotype, development and the phenotype.
Assuntos
Modelos Biológicos , Phoca , Dente/anatomia & histologia , Dente/fisiologia , Animais , Redes Reguladoras de Genes/genética , Genótipo , Fenótipo , Phoca/anatomia & histologia , Phoca/genética , Phoca/crescimento & desenvolvimento , Transdução de Sinais , Dente/crescimento & desenvolvimentoRESUMO
An increasing number of mammalian species have been shown to have a history of hybridization and introgression based on genetic analyses. Only relatively few fossils, however, preserve genetic material, and morphology must be used to identify the species and determine whether morphologically intermediate fossils could represent hybrids. Because dental and cranial fossils are typically the key body parts studied in mammalian palaeontology, here we bracket the potential for phenotypically extreme hybridizations by examining uniquely preserved cranio-dental material of a captive hybrid between grey and ringed seals. We analysed how distinct these species are genetically and morphologically, how easy it is to identify the hybrids using morphology and whether comparable hybridizations happen in the wild. We show that the genetic distance between these species is more than twice the modern human-Neanderthal distance, but still within that of morphologically similar species pairs known to hybridize. By contrast, morphological and developmental analyses show grey and ringed seals to be highly disparate, and that the hybrid is a predictable intermediate. Genetic analyses of the parent populations reveal introgression in the wild, suggesting that grey-ringed seal hybridization is not limited to captivity. Taken together, we postulate that there is considerable potential for mammalian hybridization between phenotypically disparate taxa.
RESUMO
A central aim of evolutionary developmental research is to decipher the relative roles of ecological and molecular interactions in explaining biological diversity. Tetrapod teeth show diverse evolutionary patterns with a repeated increase in dental complexity, especially in response to herbivorous habits. Most extensively in mammals, dentition increases in complexity by elaborating morphology of individual teeth rather than increasing the number of teeth. Even though evolution of mammalian dentition is governed by ecology, recent evidence on molecular signalling suggests that many details and even some general evolutionary tendencies may be instigated by development. Specifically, iterative use of the same developmental modules, the enamel knots, may have facilitated developmentally efficient, or economical, elaboration of tooth shapes without substantially compromising the existing morphology. These kinds of developmentally influenced tendencies may be hypothesized to be typical to many organs and systems showing repeated evolutionary patterns.
Assuntos
Biologia do Desenvolvimento/métodos , Dente/embriologia , Dente/fisiologia , Animais , Evolução Biológica , Simulação por Computador , Ecologia , Evolução Molecular , Genótipo , Humanos , Modelos Biológicos , Mutação , Fenótipo , Transdução de SinaisRESUMO
Teeth are a good system for studying development and evolution. Tooth development is largely independent of the rest of the body and teeth can be grown in culture to attain almost normal morphology. Their development is not affected by the patterns of movement or sensorial perception in the embryo. Teeth are hard and easily preserved. Thus, there is plenty of easily accessible information about the patterns of morphological variation occurring between and within species. This review summarises recent work and describes how tooth development can be understood as the coupling between a reaction-diffusion system and differential growth produced by diffusible growth factors: which growth factors are involved, how they affect each other's expression and how they affect the spatial patterns of proliferation that lead to final morphology. There are some aspects of tooth development, however, that do not conform to some common assumptions in many reaction-diffusion models. Those are discussed here since they provide clues about how reaction-diffusion systems may work in actual developmental systems. Mathematical models implementing what we know about tooth development are discussed.
Assuntos
Padronização Corporal/genética , Modelos Teóricos , Odontogênese/genética , Dente/crescimento & desenvolvimento , Animais , Evolução Biológica , Padronização Corporal/fisiologia , Regulação da Expressão Gênica no Desenvolvimento , Redes Reguladoras de Genes , Humanos , Dente/metabolismoRESUMO
One of the aims of developmental biology is to understand how a single egg cell gives rise to the complex spatial distributions of cell types and extracellular components of the adult phenotype. This review discusses the main genetic and epigenetic interactions known to play a role in tooth development and how they can be integrated into coherent models. Along the same lines, several hypotheses about aspects of tooth development that are currently not well understood are evaluated. This is done from their morphological consequences from the model and how these fit known morphological variation and change during tooth development. Thus the aim of this review is two-fold. On one hand the model and its comparison with experimental evidence will be used to outline our current understanding about tooth morphogenesis. On the other hand these same comparisons will be used to introduce a computational model that makes accurate predictions on three-dimensional morphology and patterns of gene expression by implementing cell signaling, proliferation and mechanical interactions between cells. In comparison with many other models of development this model includes reaction-diffusion-like dynamics confined to a diffusion chamber (the developing tooth) that changes in shape in three-dimensions over time. These changes are due to mechanical interactions between cells triggered by the proliferation enhancing effect of the reactants (growth factors). In general, tooth morphogenesis can be understood from the indirect cross-regulation between extracellular signals, the local regulation of proliferation and differentiation rates by these signals and the effect of intermediate developing morphology on the diffusion, dilution, and spatial distribution of these signals. Overall, this review should be interesting to either readers interested in the mechanistic bases of tooth morphogenesis, without necessarily being interested in modeling per se, and readers interested in development modeling in general.
Assuntos
Modelos Dentários , Odontogênese , Dente/crescimento & desenvolvimento , Animais , Evolução Biológica , Biologia do Desenvolvimento , Regulação da Expressão Gênica no Desenvolvimento , Substâncias de Crescimento/genética , Substâncias de Crescimento/fisiologia , Técnicas In Vitro , Mamíferos/genética , Mamíferos/crescimento & desenvolvimentoRESUMO
The single replacement from milk teeth to permanent teeth makes mammalian teeth different from teeth of most nonmammalian vertebrates and other epithelial organs such as hair and feathers, whose continuous replacement has been linked to Wnt signaling. Here we show that mouse tooth buds expressing stabilized beta-catenin in epithelium give rise to dozens of teeth. The molar crowns, however, are typically simplified unicusped cones. We demonstrate that the supernumerary teeth develop by a renewal process where new signaling centers, the enamel knots, bud off from the existing dental epithelium. The basic aspects of the unlocked tooth renewal can be reproduced with a computer model on tooth development by increasing the intrinsic level of activator production, supporting the role of beta-catenin pathway as an upstream activator of enamel knot formation. These results may implicate Wnt signaling in tooth renewal, a capacity that was all but lost when mammals evolved progressively more complicated tooth shapes.
Assuntos
Mucosa Bucal/metabolismo , Transdução de Sinais/fisiologia , Dente/crescimento & desenvolvimento , Proteínas Wnt/fisiologia , beta Catenina/fisiologia , Animais , Heterozigoto , Camundongos , Camundongos Transgênicos , Mucosa Bucal/fisiologia , Mutação , Técnicas de Cultura de Tecidos , Dente/embriologia , Dente/metabolismo , Proteínas Wnt/metabolismo , beta Catenina/genética , beta Catenina/metabolismoRESUMO
The neo-Darwinian paradigm benefits from the assumption that phenotypic variation is gradual and that phenotype and genotype have a relatively simple relationship. These assumptions are historically inherited from the times of the neo-Darwinian synthesis and, consequently, do not include present understanding about development. In this study, understanding about the dynamics of pattern formation is used to explore to that extent phenotypic variation can be expected to be gradual and simply related to molecular variation. Variation in simple phenotypes seems to fit neo-Darwinian assumptions but variation in complex phenotypes does not. Instead, variation in complex phenotypes would have a tendency to relatively less gradual evolution, even at microevolutionary time scales, that would make phylogenetic reconstructions more difficult. In addition, they will have a tendency to exhibit specific trends in innovation rates over group radiations with early accelerations and late decelerations. This work also explores further consequences of these results in our understanding of phenotypic evolution.
Assuntos
Evolução Biológica , Modelos Biológicos , Morfogênese , Animais , Simulação por Computador , Regulação da Expressão Gênica no Desenvolvimento , Fenótipo , Dente/anatomia & histologia , Dente/embriologia , Dente/crescimento & desenvolvimentoRESUMO
Here we investigate how development and evolution can affect each other by exploring what kind of phenotypic variation is produced by different types of developmental mechanisms. A limited number of developmental mechanisms are capable of pattern formation in development. Two main types have been identified. In morphodynamic mechanisms, induction events and morphogenetic processes, such as simple growth, act at the same time. In morphostatic mechanisms, induction events happen before morphogenetic mechanisms, and thus growth cannot influence the induction of a pattern. We present a study of the variational properties of these developmental mechanisms that can help to understand how and why a developmental mechanism may become involved in the evolution and development of a particular morphological structure. Using existing models of pattern formation in teeth, an extensive simulation analysis of the phenotypic variation produced by different types of developmental mechanisms is performed. The studied properties include the amount and diversity of the phenotypic variation produced, the complexity of the phenotypic variation produced, and the relationship between phenotype and genotype. These variational properties are so different between different types of mechanisms that the relative involvement of these types of mechanisms in evolutionary innovation and in different stages of development can be estimated. In addition, type of mechanism affects the tempo and mode of morphological evolution. These results suggest that the basic principles by which development is organized can influence the likelihood of morphological evolution.
Assuntos
Evolução Biológica , Padronização Corporal , Indução Embrionária/fisiologia , Modelos Biológicos , Dente/crescimento & desenvolvimento , Animais , Morfogênese , Fatores de Tempo , Dente/anatomia & histologiaRESUMO
Generation of morphological diversity remains a challenge for evolutionary biologists because it is unclear how an ultimately finite number of genes involved in initial pattern formation integrates with morphogenesis. Ideally, models used to search for the simplest developmental principles on how genes produce form should account for both developmental process and evolutionary change. Here we present a model reproducing the morphology of mammalian teeth by integrating experimental data on gene interactions and growth into a morphodynamic mechanism in which developing morphology has a causal role in patterning. The model predicts the course of tooth-shape development in different mammalian species and also reproduces key transitions in evolution. Furthermore, we reproduce the known expression patterns of several genes involved in tooth development and their dynamics over developmental time. Large morphological effects frequently can be achieved by small changes, according to this model, and similar morphologies can be produced by different changes. This finding may be consistent with why predicting the morphological outcomes of molecular experiments is challenging. Nevertheless, models incorporating morphology and gene activity show promise for linking genotypes to phenotypes.