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Electrokinetic DNA transport in 20 nm-high nanoslits: evidence for movement through a wall-adsorbed.
Electrophoresis
; 32(18): 2402-9, 2011 Sep.
Artigo
em Inglês
| MEDLINE
| ID: mdl-21922490
RESUMO
The electrokinetic transport behavior of λ-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.