RESUMO
Healing of complex wounds requires dressings that must, at least, not hinder and should ideally promote the activity of key healing cells, in particular fibroblasts. This in vitro study assessed the effects of three wound-dressings (a pure Ca2+ alginate: Algostéril®, a Ca2+ alginate + carboxymethylcellulose: Biatain alginate® and a polyacrylate impregnated with lipido-colloid matrix: UrgoClean®) on dermal fibroblast activity. The results showed the pure calcium alginate to be non-cytotoxic, whereas the other wound-dressings showed moderate to strong cytotoxicity. The two alginates stimulated fibroblast migration and proliferation, whereas the polyacrylate altered migration and had no effect on proliferation. The pure Ca2+ alginate significantly increased the TGF-ß-induced fibroblast activation, which is essential to healing. This activation was confirmed by a significant increase in Vascular endothelial growth factor (VEGF) secretion and a higher collagen production. The other dressings reduced these fibroblast activities. The pure Ca2+ alginate was also able to counteract the inhibitory effect of NK cell supernatants on fibroblast migration. These in vitro results demonstrate that tested wound-dressings are not equivalent for fibroblast activation. Only Algostéril was found to promote all the fibroblast activities tested, which could contribute to its healing efficacy demonstrated in the clinic.
Assuntos
Alginatos , Movimento Celular , Proliferação de Células , Fibroblastos , Fator A de Crescimento do Endotélio Vascular , Cicatrização , Fibroblastos/efeitos dos fármacos , Cicatrização/efeitos dos fármacos , Humanos , Alginatos/farmacologia , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Fator A de Crescimento do Endotélio Vascular/metabolismo , Colágeno/metabolismo , Bandagens , Fator de Crescimento Transformador beta/metabolismo , Carboximetilcelulose Sódica , Células Cultivadas , Células Matadoras Naturais/efeitos dos fármacos , Resinas Acrílicas , Ácidos Hexurônicos , Ácido Glucurônico , PeleRESUMO
The treatment of sequelae for burns or other loss of perioral tissues is complex due to the site where they occur, its functional importance, and social and esthetic aspects. Functional consequences of burns to this area are cutaneous retraction and a lack of skin that can lead to an inability to close the oral aperture, compromising the provision of dental hygiene and intubation procedures. The aim of the present publication was to evaluate the efficacy of chin, labial and jugal cutaneous expansions for the treatment of perioral lesions and lesions of the lower half of the face in our retrospective series of patients. We collected data and photography from digital files for each patient. Proportion of scarred skin that could be treated by one or several expansion procedures was evaluated. The main outcome was the resection of 50% or more initial lesions. Side effects were assessed. Out of a total of 33 expanders, 28 were at the jugal level, 5 were chin expanders, and none were labial expanders. This equated to the inclusion of fourteen patients. The average percentage of the lesion that was removed after the perioral expansion protocol was 68.9% (40%-100%). 85% of patients had a positive outcome. 12% of procedures were complicated by hematoma, infection or prosthesis exposure. Each time that the lesional area could be fully (i.e. 100%) treated, only a single expansion was used. Head and neck expansion is the technique of choice for reconstruction of the lower half of the face and the horizontal part of the neck in terms of efficiency and safety.
Le traitement des séquelles de brûlures ou d'autres pertes de substances tissulaires de la région péri buccale est complexe du fait de l'importance fonctionnelle de cette région et des conséquences esthétiques et sociales. Les conséquences des brûlures de cette région sont les rétractions ou la rigidité cutanées entrainant une limitation de la fermeture buccale et de ce fait compromettant l'état dentaire et les procédures d'intubation. Le but de ce travail est d'évaluer l'efficacité de l'expansion cutanée du menton, des lèvres et des joues dans le traitement des lésions de la région péribuccale ou de la face basse par une étude rétrospective de notre série. Nous avons repris les données cliniques et les photographies à partir des dossiers informatisés pour chaque patient. Nous avons évalué la quantité de peau cicatricielle traitée en un ou plusieurs temps. Le résultat principal est que plus de 50% de la lésion initiale a été traitée. Les effets secondaires ont été évalués. 14 patients ont été inclus. 33 expandeurs ont été posés, 28 étaient au niveau de la joue, 5 sur le menton et aucun sur les lèvres. Le pourcentage moyen de lésion excisée après expansion cutanée de la région péribuccale était de 68,9% (40%-100%). 85% des patients ont eu un résultat satisfaisant. 12% des procédures d'expansion se sont compliquées d'hématome, d'infection ou d'exposition de la prothèse. A chaque fois que la lésion pouvait être traitée totalement, une seule procédure a été réalisée. L'expansion cutanée de la tête et du cou est la technique de choix pour la reconstruction de la face basse et de la portion horizontale du cou en termes de résultats et de complications.