RESUMO
This study was investigated the role of magnesium (Mg2+) ion substituted biphasic calcium phosphate (Mg-BCP) spherical micro-scaffolds in osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells (hAT-MSCs). Mg-BCP micro-scaffolds with spherical morphology were successfully prepared using in situ co-precipitation and spray drying atomization process. The in vitro cell proliferation and differentiation of hAT-MSCs were determined up to day 14. After in vitro biological tests, Mg-BCP micro-scaffolds with hAT-MSCs showed more enhanced osteogenicity than pure hAT-MSCs as control group by unique biodegradation of TCP phase and influence of substituted Mg2+ ion in biphasic nanostructure. Therefore, these results suggest that Mg-BCP micro-scaffolds promote osteogenic differentiation of hAT-MSCs.
Assuntos
Hidroxiapatitas/química , Magnésio/química , Células-Tronco Mesenquimais/citologia , Nanosferas/química , Osteoblastos/citologia , Alicerces Teciduais , Adipócitos/citologia , Adipócitos/fisiologia , Substitutos Ósseos/síntese química , Diferenciação Celular/fisiologia , Células Cultivadas , Desenho de Equipamento , Análise de Falha de Equipamento , Humanos , Íons , Teste de Materiais , Células-Tronco Mesenquimais/fisiologia , Nanopartículas Metálicas/química , Nanopartículas Metálicas/ultraestrutura , Nanosferas/ultraestrutura , Osteoblastos/fisiologia , Osteogênese/fisiologia , Tamanho da Partícula , Propriedades de SuperfícieRESUMO
Porous hydroxyapatite (HAp)/chitosan-alginate composite scaffolds were prepared through in situ co-precipitation and freeze-drying for bone tissue engineering. The composite scaffolds were highly porous and interconnected with a pore size of around 50-220 µm at low concentrations of HAp. As the HAp content increased, the porosity of the scaffolds decreased from 84.98 to 74.54%. An MTT assay indicates that the obtained scaffolds have no cytotoxic effects on MG-63 cells, and that they have good biocompatibility. An implantation experiment in mouse skulls revealed that the composite scaffold provides a strong positive effect on bone formation in vivo in mice. Furthermore, that HAp/chitosan-alginate composite scaffold has been shown to be more effective for new bone generation than chitosan-alginate scaffold.