[Stabilization of G x C-containing DNA duplexes by polyamides with parallel orientation in the minor groove]. / Stabilizatsiia G x C-soderzhashchikh DNK-dupleksov poliamidami s parallel'noi orientatsiei v maloi borozdke.

The polyamides based on 4-amino-1-methylpyrrol-2-carboxylic acid, 4-amino-1-methylimidazole-2-carboxylic acid, and beta-alanine that stabilize oligonucleotide duplexes consisting of G x C pairs through parallel packing in the minor groove were studied. The initial duplex TTGCGCp x GCGCAA melts at 28 degrees C; the TTGCGCp[NH(CH2)3COPyIm betaImNH(CH2)3NH(CH3)2][NH(CH2)3COIm betaImPyNH(CH2)3N(CH3)2] x GCGCAA duplex (bisphosphoramidate with parallel orientation of ligands, where Py, Im, and beta are the residues of 1-methyl-4-aminopyrrol-2-carboxylic and 1-methyl-4-aminoimidazole-2-carboxylic acids and beta-alanine, respectively), at 48 degrees C; and the TTGCGCp[NH(CH2)3COIm betaImPyNH(CH2)3COIm betaImPyNH(CH2)3N(CH3)2] x GCGCAA duplex (a hairpin structure with antiparallel orientation), at 56 degrees C. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2004, vol. 30, no. 5; see also http: // www.maik.ru.

[A rapid and effective solid-phase synthesis of DNA-sequence binding polyamides]. / Bystryi i éffektivnyi tverdofaznyi sintez poliamidov, sviazyvaiushchikhsia s DNK.

A rapid and effective variant of solid-phase synthesis of DNA-sequence-specific polyamides on the basis of 4-amino-1-methylpyrrole-2-carboxylic acid, 4-amino-1-methylimidazole-2-carboxylic acid, beta-alanine, and gamma-aminobutyric acid was suggested. It is based on the use of di- and trimeric oligocarboxamide building blocks, which help reduce the time of synthesis, increase its yield and purity of products, and efficiently use manual synthesis for the synthesis of long oligocarboxamides. The yields of hairpin ligands with up to 10 units are 35-50% and the synthesis takes no more than 6 h.