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1.
BMC Oral Health ; 24(1): 378, 2024 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-38519948

RESUMO

BACKGROUND: Ameloblastoma (AM) is the most common benign odontogenic tumor, which is more often detected in the mandible than maxilla, especially the mandibular body and mandibular angle. Pediatric AM is a rare disease, especially in patients aged 10 and younger. Compared with the mainstream osteotomy and reconstructive surgery for adult ameloblastoma, there is more room for discussion in the treatment of pediatric ameloblastoma. The postoperative functional and psychological influence can not be ignored. Especially for children in the period of growth and development, an osteotomy is often challenging to be accepted by their parents. We report two patients with ameloblastoma under 10 years old who are treated with curettage and fenestration, which is a beneficial method for children with ameloblastoma. CASE PRESENTATION: We present two cases of classic ameloblastoma in children. We describe in detail the patients' characteristics, treatment processes, and follow-up result. The bone formation and reconstruction in the lesion area after fenestration decompression and curettage are recorded at every clinic review. The surgical details and principles of curettage and decompression are also described and discussed. The two patients have good bone shape recovery and no recurrence. CONCLUSIONS: Children are in the growth and development period and possess an extremely strong ability of bone formation and reconstruction. Based on the principles of minimally invasive and functional preservation, we believe that curettage combined with decompression can be the first choice for treating AM in children, especially for mandibular lesions.


Assuntos
Ameloblastoma , Neoplasias Mandibulares , Criança , Humanos , Ameloblastoma/cirurgia , Curetagem/métodos , Descompressão , Mandíbula/cirurgia , Neoplasias Mandibulares/cirurgia
2.
Luminescence ; 33(1): 119-124, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-28776941

RESUMO

A novel ratiometric fluorescence nanosensor for superoxide anion (O2•- ) detection was designed with gold nanoparticles-bovine serum albumin (AuNPs-BSA)@terbium/guanosine monophosphate disodium (Tb/GMP) nanoscale coordination polymers (NCPs) (AuNPs-BSA@Tb/GMP NCPs). The abundant hydroxyl and amino groups of AuNPs-BSA acted as binding points for the self-assembly of Tb3+ and GMP to form core-shell AuNPs-BSA@Tb/GMP NCP nanosensors. The obtained probe exhibited the characteristic fluorescence emission of both AuNPs-BSA and Tb/GMP NCPs. The AuNPs-BSA not only acted as a template to accelerate the growth of Tb/GMP NCPs, but also could be used as the reference fluorescence for the detection of O2•- . The resulting AuNPs-BSA@Tb/GMP NCP ratiometric fluorescence nanosensor for the detection of O2•- demonstrated high sensitivity and selectivity with a wide linear response range (14 nM-10 µM) and a low detection limit (4.7 nM).


Assuntos
Técnicas Biossensoriais , Fluorescência , Nanoestruturas/química , Polímeros/química , Superóxidos/análise , Animais , Ânions/análise , Bovinos , Ouro/química , Guanosina Monofosfato/química , Soroalbumina Bovina/química , Espectrometria de Fluorescência , Térbio/química
3.
Analyst ; 139(21): 5516-22, 2014 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-25195570

RESUMO

Lanthanide coordination polymers have recently emerged as very fascinating sensing materials due to their tunable structures and unique optical properties. However, a major problem concerning the applications of lanthanide coordination polymers for fluorescent sensing is their unselective recognition to analytes. In this work, a direct post-modification strategy was employed to prepare functionalized lanthanide coordination polymer nanoparticles (Phe/Tb-CPBA CPNPs) with specific response ability to hydrogen peroxide (H2O2) by using phenylalanine (Phe) as bridging ligands, terbium ions (Tb(3+)) as metal nodes and carboxyphenylboronic acids (CPBAs) as guest ligands. Phe/Tb-CPBA CPNPs emit a strong green fluorescence due to the removal of coordinated water molecules and the sensitization effect of CPBA. Upon the addition of H2O2, however, the quenched fluorescence of Phe/Tb-CPBA CPNPs can be observed owing to an intramolecular charge transfer effect. This finding led to a method for the quantitation of H2O2 in the 6 µM to 1 mM concentration range and with a detection limit at 2 µM. Because of the chemoselective H2O2-mediated oxidative deboronation, Phe/Tb-CPBA CPNPs as fluorescent sensors exhibit excellent selectivity to H2O2. Furthermore, Phe/Tb-CPBA CPNPs were successfully used to measure the level of H2O2 in urine samples and showed satisfactory results. We envision that the presented strategy could be extended to design other functionalized coordination polymers with desired functions for various biomedical applications.


Assuntos
Líquidos Corporais/química , Peróxido de Hidrogênio/análise , Elementos da Série dos Lantanídeos/química , Nanopartículas , Polímeros/química , Adulto , Humanos , Limite de Detecção , Microscopia Eletrônica de Varredura , Espectroscopia de Infravermelho com Transformada de Fourier
4.
J Colloid Interface Sci ; 608(Pt 1): 219-226, 2022 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-34626968

RESUMO

Riboflavin (RF) is one of the necessary vitamins. If human body lacks RF, it will lead to inflammation and dysfunction of mouth, lips and skin. Thus sensitive and accurate determination of RF is necessary. Here, an electroactive covalent-organic framework nanobelt (COFTFPB-Thi) with thickness of 1.4 nm was prepared by amine-aldehyde condensation reaction between thionine and 1, 3, 5-tris (p-formylphenyl) benzene, which was then grown vertically on three-dimensional porous carbon derived from kenaf stem (3D-KSC) for double signal ratiometric electrochemical detection of RF. The resulted 3D-KSC/COFTFPB-Thi showed two reduction peaks at -0.08 V and -0.23 V, which came from the reduction of COFTFPB-Thi and the conjugated structure of COFTFPB-Thi, respectively. In the presence of RF, those RF molecules near the electrode surface were oxidized at 0.6 V. Then some oxidized RF (RFox) adsorbed on COFTFPB-Thi would oxidize COFTFPB-Thi into COFTFPB-Thi(ox) while other RFox adsorbed on 3D-KSC kept unchanged. When the potential was scanned from 0.6 V to -0.6 V, both COFTFPB-Thi(ox) and RFox adsorbed on 3D-KSC were reduced at -0.08 V and -0.45 V accordingly, while the reduction peak of -0.23 V of the conjugated structure of COFTFPB-Thi kept constant. When j-0.45/j-0.23 was used as the response signal, the detection limit was 44 nM and the linear range was 0.13 µM -0.23 mM. By using j-0.08/j-0.23 as the response signal, a detection limit of 90 nM and a linear range of 0.30 µM-0.23 mM (S/N = 3) were obtained. By using double signals, the measurement results can be corrected to make the results more accurate and reliable. The sensor also showed good selectivity, reproducibility and stability, which provided a good application prospects.


Assuntos
Técnicas Biossensoriais , Estruturas Metalorgânicas , Carbono , Técnicas Eletroquímicas , Eletrodos , Ouro , Humanos , Limite de Detecção , Fenotiazinas , Reprodutibilidade dos Testes , Riboflavina
5.
Microsc Res Tech ; 71(6): 409-12, 2008 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-18431800

RESUMO

Hybrid materials of polyacrylamide networks and gold nanoparticles were prepared by directly heating an aqueous solution containing HAuCl(4), acrylamide, N,N'-methylenebis-acrylamide, and sodium sulfite (Na(2)SO(3)). Acrylamide, N,N'-methylenebis-acrylamide, and Na(2)SO(3) were used as monomers, crosslinking agent, and initiator, respectively. In the process of polyacrylamide network synthesis, HAuCl(4) was reduced by acrylamide and Na(2)SO(3) into gold nanoparticles and adsorbed on the produced polyacrylamide networks. Transmission electron microscopy proved that the size of gold nanoparticles was in the range of 3-10 nm. Atomic force microscopy showed that the gold nanoparticles homogeneously dispersed into the polyacrylamide networks matrix. The hybrid materials as absorbents may be useful in healthcare, communication technology, building industry, chromatography, water purification, and agriculture.


Assuntos
Resinas Acrílicas/química , Ouro/química , Nanopartículas/química , Polímeros/química , Microscopia de Força Atômica , Microscopia Eletrônica de Transmissão
6.
J Phys Chem B ; 111(2): 461-8, 2007 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-17214498

RESUMO

Recently, we have successfully constructed flat-lying DNA monolayers on a mica surface (J. Phys. Chem. B 2006, 110, 10792-10798). In this work, the effects of various factors including bridge ions, DNA species, and developing temperature on the configuration of DNA monolayers have been investigated by atomic force microscopy (AFM) in detail. AFM results show that the species of bridge ions and developing temperature play a crucial role during the formation process. For example, the divalent cation Zn2+ resulted in many DNA chains stuck side by side in the monolayers due to the strong interactions between it and DNA's bases or the mica surface. Most DNA chain's conglutinations disappeared when the developing temperature was higher than 40 degrees C. Cd2+ and Ca2+ produced more compact DNA monolayers with some obvious aggregations, especially for the DNA monolayers constructed by using Ca2+ as the bridge ion. Co2+ produced well-ordered, flat-lying DNA monolayers similar to that of Mg2+. Furthermore, it was found that the flat-lying DNA monolayers could still form on a mica surface when plasmid DNA pBR 322 and linear DNA pBR 322/Pst I were used as the DNA source. Whereas, it was hard to form DNA monolayers on a (3-aminopropyl)triethoxysilane-mica surface because the strong interactions between DNA and substrate prevented the lateral movement of DNA molecules. These results suggested that the appropriate interactions between divalent cations and DNA or mica surface were important for the formation of flat-lying DNA monolayers. The obtained information is a necessary supplement to our previous studies on the formation kinetics of such monolayers and may be useful for practical application of the monolayers and further theoretical studies.


Assuntos
DNA/química , Membranas Artificiais , Metais/química , Temperatura , Íons/química , Microscopia de Força Atômica/métodos , Tamanho da Partícula , Sensibilidade e Especificidade , Relação Estrutura-Atividade , Propriedades de Superfície
7.
Microsc Res Tech ; 69(12): 998-1004, 2006 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-16981196

RESUMO

A modified tapping mode of the atomic force microscope (AFM) was introduced for manipulation, dissection, and lithography. By sufficiently decreasing the amplitude of AFM tip in the normal tapping mode and adjusting the setpoint, the tip-sample interaction can be efficiently controlled. This modified tapping mode has some characteristics of the AFM contact mode and can be used to manipulate nanoparticles, dissect biomolecules, and make lithographs on various surfaces. This method did not need any additional equipment and it can be applied to any AFM system.


Assuntos
Microscopia de Força Atômica/métodos , Nanoestruturas/ultraestrutura , Nanotecnologia/métodos , Dissecação , Ouro , Plasmídeos/ultraestrutura , Poliestirenos
8.
Microsc Res Tech ; 66(2-3): 156-62, 2005 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-15880504

RESUMO

By controlling the interaction between the atomic force microscope tip and mica, patterns of different sizes and shape have been produced on the surface of mica. Using these operator-constructed patterns as a reliable marker, the original scanned sample location can be re-located and imaged again on the same mica surface by atomic force microscopy (AFM). This location technique can be used to find the same object again even if the sample was removed from the AFM instrument or the sample was imaged in a different mode.


Assuntos
Silicatos de Alumínio/química , Microscopia de Força Atômica/métodos , Microscopia de Força Atômica/instrumentação , Microesferas , Plasmídeos , Poliestirenos , Propriedades de Superfície
9.
Biophys Chem ; 105(1): 1-9, 2003 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-12932574

RESUMO

Calf-thymus DNA-incorporated bilayer lipid membranes supported on a glassy carbon (GC) electrode was prepared by making layers of phosphatidylcholine dimyristoyl (DMPC) on GC electrode. DNA in the BLM was characterized by cyclic voltammetry, IR and AFM, and lipid layers formed on the GC electrode were demonstrated to be a bilayer lipid membrane by electrochemical impedance experiment. In IR and AFM experiments the findings indicated that DNA was incorporated into BLM. The ion channel of bilayer lipid membranes incorporated was studied. The result showed that the ion channel was opened in the presence of the stimulus quinacrine. In the absence of quinacrine the channel was switched. The process can repeat itself many times. The impedance spectroscopy measurements demonstrate that the stimulus quinacrine opens the channel for permeation of marker ion. The mechanism of forming an ion channel was investigated.


Assuntos
Materiais Revestidos Biocompatíveis/química , DNA/química , Bicamadas Lipídicas/química , Animais , Carbono/química , Bovinos , Dimiristoilfosfatidilcolina/química , Impedância Elétrica , Eletroquímica/métodos , Eletrodos , Ferricianetos/química , Canais Iônicos/química , Canais Iônicos/efeitos dos fármacos , Microscopia de Força Atômica , Quinacrina/farmacologia , Espectroscopia de Infravermelho com Transformada de Fourier , Análise Espectral/métodos
10.
Biosens Bioelectron ; 50: 447-52, 2013 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-23907076

RESUMO

The meta-organic coordination polymers have been emerged as fascinating nanomaterials because of their tunable nature. In this work, we employed lanthanide coordination polymer self-assembled from adenosine monophosphate (AMP) and europium ion (Eu(3+)) as receptor reagent and citrate (Cit) as ancillary ligand to construct a fluorescent sensor for the detection of tetracycline (Tc) in milk. The co-coordination of Cit and Tc with Eu(3+) on the surface of the coordination polymer AMP/Eu leads to the formation of ternary complex which emitted strong fluorescence due to the removal of coordinated water molecules and an intramolecular energy transfer from Tc to Eu(3+). The fluorescent intensity of Eu(3+) displayed a good linear response to Tc concentrations in the range of 0.1-20 µM with a detection limit of 60 nM. This method was successfully applied to determine the levels of Tc in milk, which is the first application of coordination polymer as a fluorescent sensor in real sample. Compared with other Eu(3+)-based fluorescent methods for Tc detection, the presented method allows simple, direct analysis of Tc without requiring special reaction media or complicated prepreparation processes. This straightforward strategy could be extended to the preparation of other lanthanide coordination polymer-based fluorescent probes for applications in biosensing, imaging, drug delivery, and so on.


Assuntos
Antibacterianos/análise , Európio/química , Corantes Fluorescentes/química , Leite/química , Polímeros/química , Tetraciclina/análise , Monofosfato de Adenosina/química , Animais , Ácido Cítrico/química , Complexos de Coordenação/química , Limite de Detecção , Espectrometria de Fluorescência/métodos
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