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1.
Colloids Surf B Biointerfaces ; 201: 111628, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33639509

RESUMO

Novel polysaccharide-platinum conjugated polymers bearing alendronate on Portulaca oleracea polysaccharides (PPS) were designed and synthesized. Their chemical structures and properties were characterized by Fourier transform infrared spectroscopy (FT-IR), 1H NMR and 31P NMR spectroscopy, Thermogravimetric analysis (TGA), X-ray powder diffraction (XRD), UV-vis spectrophotometer (UV-vis) and other analysis methods. The results demonstrated that alendronate can be used as the linker of Portulaca oleracea polysaccharides and platinum compounds. Portulaca oleracea polysaccharides-alendronate (PPS-ALN) conjugates exhibited stronger antioxidant ability than PPS. The cytotoxicity assay to cancer cells was tested in vitro, and the Portulaca oleracea polysaccharides-alendronate-platinum (PPS-ALN-Pt) conjugates strongly inhibited the proliferation of cancer cells than PPS and PPS-ALN. The evaluation of complexes affinity toward supercoiled plasmid DNA, displayed a high DNA interaction. Interestingly, the platinum conjugates displayed immunological competence in HeLa cells by cellular immunofluorescence assay. Besides, the cellular platinum accumulation of PPS-ALN-Pt conjugates was higher than that of cisplatin in HeLa cells, implying that the polysaccharide-platinum conjugated polymers might have a synergistically therapeutic application in metal anticancer drug delivery.


Assuntos
Preparações Farmacêuticas , Portulaca , Células HeLa , Humanos , Platina , Polímeros , Polissacarídeos , Espectroscopia de Infravermelho com Transformada de Fourier
2.
J Neurotrauma ; 27(1): 263-74, 2010 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-19691422

RESUMO

Traumatic spinal cord injury (SCI) causes marked neuropathological changes in the spinal cord, resulting in limited functional recovery. Currently, there are no effective treatments, and the mechanisms underlying these neuropathological changes are not completely understood. In this study, two-dimensional gel electrophoresis coupled with mass spectrometry was used to investigate injury-related changes in the abundance (SYPRO Ruby stain) and phosphorylation (Pro-Q Diamond stain) of proteins from the soluble fraction of the lesion epicenter at 24 h following SCI. Over 1500 SYPRO Ruby-stained spots and 100 Pro-Q Diamond-stained spots were examined. We identified 26 unique proteins within 38 gel spots that differentially changed in abundance, phosphorylation, or both in response to SCI. Protein redundancies among the gel spots were likely due to differences in proteolysis, post-translational modifications, and the existence of isoforms. The proteins affected were blood-related proteins, heat-shock proteins, glycolytic enzymes, antioxidants, and proteins that function in cell structure, cell signaling, DNA damage, and protein degradation. These protein changes post injury may suggest additional avenues of investigation into the underlying molecular mechanisms responsible for the pathophysiological consequences of SCI.


Assuntos
Proteínas do Tecido Nervoso/metabolismo , Proteômica/métodos , Traumatismos da Medula Espinal/metabolismo , Medula Espinal/metabolismo , Animais , Dimetilpolisiloxanos , Modelos Animais de Doenças , Eletroforese em Gel Bidimensional , Metabolismo Energético/fisiologia , Feminino , Glicerol , Hemorragia/metabolismo , Hemorragia/fisiopatologia , Espectrometria de Massas , Degeneração Neural/metabolismo , Degeneração Neural/fisiopatologia , Proteínas do Tecido Nervoso/análise , Compostos Organometálicos , Fosfoproteínas/análise , Fosfoproteínas/metabolismo , Fosforilação , Ratos , Ratos Long-Evans , Transdução de Sinais/fisiologia , Medula Espinal/patologia , Medula Espinal/fisiopatologia , Traumatismos da Medula Espinal/patologia , Traumatismos da Medula Espinal/fisiopatologia , Coloração e Rotulagem , Frações Subcelulares/metabolismo , Fatores de Tempo
3.
Langmuir ; 20(12): 4926-32, 2004 Jun 08.
Artigo em Inglês | MEDLINE | ID: mdl-15984252

RESUMO

Understanding the influence of molecular environment on phospholipids is important in time-of-flight secondary ion mass spectrometry (TOF-SIMS) studies of complex systems such as cellular membranes. Varying the molecular environment of model membrane Langmuir-Blodgett (LB) films is shown to affect the TOF-SIMS signal of the phospholipids in the films. The molecular environment of a LB film of dipalmitoylphosphatidylcholine (DPPC) is changed by varying the film density, varying the sample substrate, and the addition of cholesterol. An increase in film density results in a decrease in the headgroup fragment ion signal at a mass-to-charge ratio of 184 (phosphocholine). Varying the sample substrate increases the secondary ion yield of phosphocholine as does the addition of proton-donating molecules such as cholesterol to the DPPC LB film. Switching from a model system of DPPC and cholesterol to one of dipalmitoylphosphatidylethanolamine (DPPE) and cholesterol demonstrates the ability of cholesterol to also mask the phospholipid headgroup ion signal. TOF-SIMS studies of simplistic phospholipid LB model membrane systems demonstrate the potential use of these systems in TOF-SIMS analysis of cells.


Assuntos
Fosforilcolina/análise , Fosforilcolina/química , Espectrometria de Massa de Íon Secundário , Colesterol/química , Membranas Artificiais , Modelos Biológicos , Fosfatidiletanolaminas/química
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