RESUMO
The aim of the investigation was to study the integration between native human dental tissue and new-generation biomimetic materials replicating the mineral-organic complex of dentin and enamel using IR microspectroscopy for multidimensional visualization and analysis. MATERIALS AND METHODS: The conditions for stable integration at the interface between biomimetic material and natural hard tissue were identified using a biocomposite buffer system of nanocrystalline carbonate-substituted calcium hydroxyapatite corresponding in its total characteristics to human dentin-enamel apatite and a number of amino acids present in the organic matrix of dentin and enamel: L-histidine, L-lysine hydrochloride, L-arginine hydrochloride, and hyaluronic acid. The finished samples were studied using IR microspectroscopy on IRM channel equipment (The Australian Synchrotron, Melbourne, Australia). RESULTS: The characteristic features of the biomimetic buffer layer at the interface between the enamel and dental material were revealed and visualized based on IR mapping of absorption intensity for particular functional molecular groups with the use of synchrotron radiation, location of the functional groups involved in the processes of biomimetic composite integration was identified.