RESUMO
Many biopolymers exhibit a strong complexing ability for multivalent ions. Often such ions form ionic bridges between the polymer chains. This leads to the formation of ionic cross linked networks and supermolecular structures, thus promoting the modification of the behavior of solid and gel polymer networks. Sorption of biopolymers on fiber surfaces and interfaces increases substantially in the case of multivalent ions, e.g., calcium being available for ionic crosslinking. Through controlled adsorption and ionic crosslinking surface modification of textile fibers with biopolymers can be achieved, thus altering the characteristics at the interface between fiber and surrounding matrices. A brief introduction on the differences deriving from the biopolymers, as their interaction with other compounds, is given. Functional models are presented and specified by several examples from previous and recent studies. The relevance of ionic crosslinks in biopolymers is discussed by means of selected examples of wider use.
Assuntos
Biopolímeros/química , Têxteis/análise , Adsorção , Íons , Estrutura MolecularRESUMO
Transient gene expression (TGE) from mammalian cells is an increasingly important tool for the rapid production of recombinant proteins for research applications in biochemistry, structural biology, and biomedicine. Here we review methods for the transfection of human embryo kidney (HEK-293) and Chinese hamster ovary (CHO) cells in suspension culture using the cationic polymer polyethylenimine (PEI) for gene delivery.
Assuntos
Portadores de Fármacos/metabolismo , Vetores Genéticos/administração & dosagem , Polietilenoimina/metabolismo , Transfecção/métodos , Animais , Células CHO , Técnicas de Cultura de Células/instrumentação , Cricetinae , Cricetulus , Desenho de Equipamento , Células HEK293 , Humanos , Proteínas Recombinantes/genética , Vírus/genéticaRESUMO
Transient gene expression (TGE) is a rapid method for the production of recombinant proteins in mammalian cells. While the TGE volumetric productivity has improved significantly over the past decade, the amount of plasmid DNA (pDNA) needed for transfection remains very high. Here, we examined the use of non-specific (filler) DNA to partially replace the transgene-bearing plasmid DNA (coding pDNA) in transfections of Chinese hamster ovary (CHO) and human embryo kidney (HEK-293E) cells. When the optimal amount of coding pDNA for either host was reduced by 67% and replaced with filler DNA, the recombinant protein yield decreased by only 25% relative to the yield in control transfections. Filler DNA did not affect the cellular uptake or intracellular stability of coding pDNA, but its presence lead to increases of the percentage of transfected cells and the steady-state level of transgene mRNA compared to control transfections. Studies of the physicochemical properties of DNA-polyethyleneimine (PEI) complexes with or without filler DNA did not reveal any differences in their size or surface charge. The results suggest that filler DNA allows the coding pDNA to be distributed over a greater number of DNA-PEI complexes, leading to a higher percentage of transfected cells. The co-assembly of filler DNA and coding pDNA within complexes may also allow the latter to be more efficiently utilized by the cell's transcription machinery, resulting in a higher level of transgene mRNA.
Assuntos
DNA/genética , Plasmídeos/genética , Proteínas Recombinantes/biossíntese , Transfecção/métodos , Animais , Células CHO , Cricetinae , Cricetulus , DNA/química , DNA/metabolismo , Expressão Gênica , Células HEK293 , Humanos , Polietilenoimina , RNA Mensageiro/análise , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Proteínas Recombinantes/genéticaRESUMO
The swelling ability of kappa-carrageenan (KC) hydrogels was investigated in simulated body fluid (SBF). The SBF mimics the ionic concentrations in the vasa deferentia of human males. The study clarifies if these hydrogels can be adjusted to occlude the vasa deferentia by swelling. For this purpose, swelling to twice the initial volume is desirable. In this study, hydrogels of different primary potassium concentrations, biopolymer concentrations and ethanol-exchanged gels, were immersed in SBF either directly or after drying (pre-dried). We measured the absolute and relative swelling degree, and the swelling rates of the gels. Extensive pre-drying leads to irreversible gel densification and absolute swelling magnitudes decrease. We found that immersion into the SBF also leads to potassium ion accumulation, and network restructuring in the hydrogels. This markedly increases the storage moduli of the gel networks. The ion content in the gel structures also directly affects the swelling speed, the fastest swelling occurred in ethanol-exchanged and pre-dried gels. We found that by pre-drying and potassium content adjustment, swelling of the hydrogels is sufficient to render KC hydrogels as a possible candidate for the occlusion of the vasa deferentia.
Assuntos
Líquidos Corporais , Hidrogéis , Biopolímeros , Carragenina/química , Etanol , Humanos , Hidrogéis/química , Íons , PotássioRESUMO
Recombinant proteins are of great commercial and scientific interest. Yet, most production methods in mammalian cells involve the time- and labor-consuming step of creating stable cell lines. Production methods based on transient gene expression are advantageous in terms of speed and versatility; yet, depending on the transfection protocol, transient transfection faces some bottlenecks such as a priori complex formation, limitations in terms of transfection and production media used and the need for medium exchange prior to and/or after transfection. Published protocols for transfection of suspension-adapted HEK-293 cells with polyethyleneimine have shown great promise in overcoming some of these bottlenecks, but still require a priori complex formation for optimal yields and limit the choice of transfection and production media. Here, we report successful in situ transfection of suspension-adapted HEK-293 cells with 25-kDa linear polyethyleneimine at densities up to 20 x 10(6) cells/mL in complex media followed by production at lower cell densities (1 x 10(6) cells/mL). After concentrating cells to such high densities, transfection of HEK-293 cells becomes possible in most commonly used media and is not restricted to a specific medium. Furthermore, there is no need to make transfection complexes a priori, a step that prevents inline sterile filtration of the DNA bulk for transfection, an important consideration when scaling processes up to 100 or 1,000 L. Finally, transfecting HEK-293 cells at high density in complex media is superior to existing transfection protocols and doubles yields of recombinant protein obtainable by transient gene expression.
Assuntos
DNA/química , DNA/genética , Portadores de Fármacos/química , Rim/fisiologia , Polietilenoimina/química , Engenharia de Proteínas/métodos , Proteínas Recombinantes/metabolismo , Transfecção/métodos , Linhagem Celular , HumanosRESUMO
Transfection with polyethylenimine (PEI) was evaluated as a method for the generation of recombinant Chinese hamster ovary (CHO DG44) cell lines by direct comparison with calcium phosphate-DNA coprecipitation (CaPO4) using both green fluorescent protein (GFP) and a monoclonal antibody as reporter proteins. Following transfection with a GFP expression vector, the proportion of GFP-positive cells as determined by flow cytometry was fourfold higher for the PEI transfection as compared to the CaPO4 transfection. However, the mean level of transient GFP expression for the cells with the highest level of fluorescence was twofold greater for the CaPO4 transfection. Fluorescence in situ hybridization on metaphase chromosomes from pools of cells grown under selective pressure demonstrated that plasmid integration always occurred at a single site regardless of the transfection method. Importantly, the copy number of integrated plasmids was measurably higher in cells transfected with CaPO4. The efficiency of recombinant cell line recovery under selective pressure was fivefold higher following PEI transfection, but the average specific productivity of a recombinant antibody was about twofold higher for the CaPO4-derived cell lines. Nevertheless, no difference between the two transfection methods was observed in terms of the stability of protein production. These results demonstrated the feasibility of generating recombinant CHO-derived cell lines by PEI transfection. However, this method appeared inferior to CaPO4 transfection with regard to the specific productivity of the recovered cell lines.
Assuntos
Células CHO , Fosfatos de Cálcio/farmacologia , Expressão Gênica/efeitos dos fármacos , Polietilenoimina/farmacologia , Proteínas Recombinantes/genética , Transfecção/métodos , Animais , Fosfatos de Cálcio/química , Precipitação Química , Cricetinae , Cricetulus , DNA/análise , DNA/genética , Feminino , Citometria de Fluxo , Dosagem de Genes/efeitos dos fármacos , Marcação de Genes/métodos , Genes Reporter/efeitos dos fármacos , Vetores Genéticos/efeitos dos fármacos , Vetores Genéticos/metabolismo , Proteínas de Fluorescência Verde/biossíntese , Proteínas de Fluorescência Verde/genética , Indicadores e Reagentes , Plasmídeos/efeitos dos fármacos , Plasmídeos/metabolismo , Polietilenoimina/química , Proteínas Recombinantes/biossíntese , Transgenes/efeitos dos fármacosRESUMO
The kinetics of polyethylenimine (PEI)-mediated gene transfer at early times after transfection of Chinese hamster ovary (CHO) cell in suspension were investigated using a novel in vitro assay. Addition of an excess of competitor DNA to the culture medium at various times after the initiation of transfection inhibited further cellular uptake of PEI-DNA particles. Using this approach, a constant rate of particle uptake was observed during the first 60 min of transfection at a PEI:DNA ratio of 2:1 (w/w) and a cell density of 2 x 10(6) cells/ml under serum-free conditions. The uptake rate declined considerably during the next 2 h of transfection. Both the rate and the level of PEI-DNA uptake in serum-free minimal medium were found to be dependent on the PEI-DNA ratio, the cell density at the time of transfection, and the extent of particle aggregation. These studies of the early phase of PEI-mediated transfection are expected to lead to further opportunities for optimization of gene transfer to suspension cultures of mammalian cells for the purpose of large-scale transient recombinant protein production.
Assuntos
Polietilenoimina , Transfecção/métodos , Animais , Células CHO , Técnicas de Cultura de Células , Cricetinae , Cricetulus , DNA , Cinética , PlasmídeosRESUMO
Large-scale transient gene expression in mammalian cells is being developed for the rapid production of recombinant proteins for biochemical and preclinical studies. Here, the scalability of transient production of a recombinant human antibody in Chinese hamster ovary (CHO) cells was demonstrated in orbitally shaken disposable bioreactors at scales from 50 mL to 50 L. First, a small-scale multiparameter approach was developed to optimize the poly(ethylenimine)-mediated transfection in 50 mL shake tubes. This study confirmed the benefit, both in terms of extended cell culture viability and increased product yield, of mild hypothermic cultivation conditions for transient gene expression in CHO cells. Second, the scalability of the process was demonstrated in disposable shake bioreactors having nominal volumes of 5, 20, and 50 L with final antibody yields between 30 and 60 mg L(-1). Thus, the combination of transient gene expression with disposable shake bioreactors allows for rapid and cost-effective production of recombinant proteins in CHO cells.
Assuntos
Reatores Biológicos , Imunoglobulina G/biossíntese , Proteínas Recombinantes/biossíntese , Animais , Células CHO , Cricetinae , Cricetulus , DNA/administração & dosagem , Iminas/administração & dosagem , Imunoglobulina G/genética , Polietilenos/administração & dosagemRESUMO
Here a simple in vitro assay was used to investigate the disassembly of nanoparticles of polyethylenimine (PEI) and DNA. Particles were formed with various PEIs, allowed to mature for 10 min, and then exposed to different competitors (RNA, DNA, BSA or heparin) or to different conditions of pH or osmolarity. DNA release was determined by gel electrophoresis or spectroscopy. The presence of heparin or high salt yielded complete particle disassembly for all PEIs tested. The addition of RNA to particles formed with linear PEIs or branched 2 kDa PEI resulted in rapid DNA release, but RNA induced only partial disassembly of particles formed with large branched PEIs. In the presence of competitor DNA, slow disassembly was observed with particles made with linear PEIs or branched 2 kDa PEI but not for particles made with larger branched PEIs. The presence of BSA resulted in partial disassembly of PEI-DNA particles, but acidic pH did not affect particle stability. If particles were allowed to mature longer than 10 min in NaCl, subsequent heparin-mediated DNA release decreased as the incubation time and the PEI:DNA ratio increased. However, particles that matured in culture medium were disassembled by heparin independently of maturation time or PEI:DNA ratio. It was concluded that branched PEIs have a higher affinity for DNA than linear PEIs, that the intracellular disassembly of PEI-DNA particles may involve interactions between PEI and cellular RNA, and that extended maturation of PEI-DNA particles in NaCl prior to transfection may limit the intracellular release of plasmid DNA.
Assuntos
DNA/administração & dosagem , Técnicas de Transferência de Genes , Polietilenoimina/química , DNA/genética , Desoxirribonucleases/química , Sistemas de Liberação de Medicamentos , Eletroforese em Gel de Poliacrilamida , Heparina/química , Concentração de Íons de Hidrogênio , Conformação Molecular , Nanopartículas , Concentração Osmolar , Proteínas/química , Proteínas/genética , RNA/química , Soroalbumina Bovina/químicaRESUMO
Transient gene expression (TGE) in human embryonic kidney (HEK-293) and Chinese hamster ovary (CHO) cells is a well-established technology for the rapid generation of recombinant proteins. Although the maximum TGE yields have reached 1 g/L or more, the amount of plasmid DNA (pDNA) required for transfection remains high. Although greater than 10(3) copies of pDNA are present per transfected cell, protein yields are still lower than those achieved in recombinant cell lines with only one or a few copies of the transgene. This indicates a clear limitation to TGE in terms of the maximum level of recombinant protein production. In this study, we investigated the limitations to high-yielding TGE processes with CHO and HEK-293E cells using a monoclonal antibody as a model protein. For either cell host, both the intracellular and intranuclear pDNA levels increased linearly with the amount of pDNA added to the culture. In contrast, transgene mRNA accumulation reached a plateau as the intranuclear pDNA amount increased, suggesting a limitation in pDNA transcription. A post-transcriptional limitation to TGE yields was revealed by calculating the amount of antibody produced per transgene mRNA (mRNA utilization). For both hosts the transgene mRNA utilization decreased dramatically when transfected pDNA amounts increased beyond the level giving the maximum protein yield. The post-transcriptional limitation did not appear to be due to bottlenecks in antibody assembly or secretion, suggesting that transgene mRNA translation may be limiting. The results show that TGE yields are not limited by pDNA delivery into the nuclei, but in pDNA and transgene mRNA utilization.
Assuntos
Polietilenoimina/química , Proteínas Recombinantes/metabolismo , Transfecção/métodos , Animais , Células CHO , Cricetinae , Cricetulus , DNA/genética , DNA/farmacocinética , Células HEK293 , Humanos , Imunoglobulina G/genética , Imunoglobulina G/metabolismo , Plasmídeos/genética , Plasmídeos/farmacocinética , Proteínas Recombinantes/genéticaRESUMO
Although the protein yields from transient gene expression (TGE) with Chinese hamster ovary (CHO) cells have recently improved, the amount of plasmid DNA (pDNA) needed for transfection remains relatively high. We describe a strategy to reduce the pDNA amount by transfecting CHO-DG44 cells with 0.06 µg pDNA/10(6) cells (10% of the optimal amount) in the presence of nonspecific (filler) DNA and various polar solvents including dimethylsufoxide, dimethyl formamide, acetonitrile, dimethyl acetamide (DMA), and hexamethyl phosphoramide (HMP). All of the polar solvents with the exception of HMP increased the production of a recombinant antibody in comparison to the untreated control transfection. In the presence of 0.25% DMA, the antibody yield in a 7-day batch culture was 500 mg/L. This was fourfold higher than the yield from the untreated control transfection. Mechanistic studies revealed that the polar solvents did not affect polyethylenimine-mediated pDNA delivery into cells or nuclei. The steady-state transgene mRNA level was elevated in the presence of each of the polar solvents tested, while the transgene mRNA half-life remained the same. These results indicated that the polar solvents enhanced transgene transcription. When screening a panel of recombinant antibodies and Fc-fusion proteins for production in the presence of the polar solvents, the highest increase in yield was observed following DMA addition for 11 of the 12 proteins. These results are expected to enhance the applicability of high-yielding TGE processes with CHO-DG44 cells by decreasing the amount of pDNA required for transfection.
Assuntos
DNA/química , DNA/isolamento & purificação , Plasmídeos/química , Plasmídeos/isolamento & purificação , Transfecção/métodos , Animais , Células CHO , Cricetinae , Cricetulus , Eletroforese em Gel de Poliacrilamida , Interações Hidrofóbicas e Hidrofílicas , Polietilenoimina/química , Solventes/químicaRESUMO
The High Five (H5) cell line, derived from the lepidopteran Trichoplusia ni, is one of the major insect cell hosts for the production of recombinant proteins using the baculovirus expression vector system (BEVS). Here, we describe a simple polyethylenimine (PEI)-based transient gene expression (TGE) process for the rapid production of recombinant proteins from suspension-adapted H5 cells. The method was optimized using two model proteins, enhanced green fluorescent protein (EGFP) and human tumor necrosis factor receptor-Fc fusion protein (TNFR-Fc). After screening several promoter and enhancer combinations for high levels of TNFR:Fc production, an expression vector containing the Autographa californica multicapsid nucleopolyhedrovirus immediate early 1 (ie1) promoter and homologous region 5 (hr5) enhancer was selected. Cells were transfected at a density of 2×10(6) cells/mL by direct addition of DNA and PEI. Under optimized conditions, a 90% transfection efficiency (percentage of EGFP-positive cells) was obtained. In addition, we observed volumetric TNFR-Fc yields over 150µg/mL within 4 days of transfection. The method was found to be reproducible and scalable to 300mL. This plasmid-based transient transfection process is a simple and efficient alternative to the BEVS for recombinant protein production in H5 cells.
Assuntos
Expressão Gênica , Lepidópteros/citologia , Plasmídeos/metabolismo , Transfecção/métodos , Animais , Contagem de Células , Linhagem Celular , DNA/metabolismo , Humanos , Polietilenoimina/química , Regiões Promotoras Genéticas , Reprodutibilidade dos Testes , Suspensões , TransgenesRESUMO
In tissue engineering, degradable or non-degradable polymer matrices can act as cell-carrier-scaffolds. Cell adhesion and growth on these scaffolds can be promoted by immobilizing extracellular matrix proteins. Therefore, in this study, polymer poly(ethylene terephthalate) (PET) films were surface modified by graft polymerization of acrylic acid, to subsequently allow collagen (types I and III) immobilization and human smooth muscle cell expansion. The surfaces of PET were activated by plasma, followed by acrylic acid graft polymerization, resulting in covalently bound brushes, containing an average of either 0.22+/-0.1 or 5.93+/-0.87 microg/cm2 of poly(acrylic acid) (PAA). Subsequent electrostatic adsorption of collagen gave a surface concentration of 4.96 and 17.2 microg/cm2, respectively, as determined using radiolabelled 125I collagen. Both PET films grafted with 0.22 microg/cm2 of PAA with or without adsorbed collagen were apt for smooth muscle cell adhesion and proliferation. However, films grafted with 5.93 microg/cm2 were not. PAA-grafted PET films, onto which serum proteins of the culture medium adsorbed spontaneously, proved to be better matrices than films on which collagen has been immobilized. It, therefore, can be speculated that other serum proteins are more important than collagen for the human smooth muscle cell adhesion and growth on surface-modified polymer matrices.
Assuntos
Materiais Biocompatíveis , Músculo Liso/citologia , Polietilenotereftalatos , Bexiga Urinária/química , Resinas Acrílicas , Adesão Celular , Divisão Celular , Células Cultivadas , Células Imobilizadas , Colágeno , Humanos , Teste de Materiais , Microscopia Eletrônica de Varredura , Propriedades de Superfície , Engenharia TecidualRESUMO
A method for virus-free transient gene expression from suspension-adapted Sf9 insect cells was developed with the gene of interest being expressed from a plasmid carrying the homologous region 5 enhancer (hr5) and the immediate early 1 (ie1) promoter from Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV). Under the optimal conditions described in the study, cells were transfected at a density of 30×106 cells/mL with 0.9 µg DNA and 1.35 µg of linear 25 kD polyethylenimine (PEI) per million cells. Following transfection, the culture was diluted to 4×106 cells/mL for the protein production phase. The volumetric yield of tumor necrosis factor receptor (ectodomain) fused to an Fc domain (TNFR-Fc) was about 100 µg/mL for cultures at volumes up to 300 mL. As expected, the molecular weight of the dimeric TNFR-Fc produced from Sf9 cells was about 6 kDa less than that produced from a recombinant Chinese hamster ovary (CHO) cell line due to differences in glycosylation between the two hosts. Transient transfection provides an alternative to the baculovirus expression vector system (BEVS) for the rapid production of recombinant proteins from Sf9 cells.
Assuntos
Proteínas Recombinantes/análise , Proteínas Recombinantes/metabolismo , Células Sf9/metabolismo , Transfecção/métodos , Animais , Células CHO , Contagem de Células , Técnicas de Cultura de Células , Cricetinae , Cricetulus , Vetores Genéticos/genética , Nucleopoliedrovírus/genética , Plasmídeos/genética , Polietilenoimina , Regiões Promotoras Genéticas/genética , Receptores do Fator de Necrose Tumoral/análise , Receptores do Fator de Necrose Tumoral/química , Receptores do Fator de Necrose Tumoral/genética , Receptores do Fator de Necrose Tumoral/metabolismo , Proteínas Recombinantes/química , Proteínas Recombinantes/genéticaRESUMO
The large-scale transfection of mammalian cells allows moderate (milligram to gram) amounts of recombinant proteins (r-proteins) to be obtained for fundamental or clinical research. In this article, we describe a one-liter transfection using polyethyleneimine (PEI) for DNA delivery into human embryonic kidney (HEK-293) cells cultivated in serum-free suspension to produce a recombinant human monoclonal antibody that yields up to about 1 g/L in a 10-day process. The method is based on a DNA delivery step performed at high cell density (20×10(6) cells/mL) by direct addition of DNA and PEI to the culture. Subsequently, the cells are diluted 20-fold for the 10-day production phase in the presence of valproic acid (VPA), a histone deacetylase inhibitor. The methods for plasmid purification, antibody quantification by enzyme-linked immunosorbent assay (ELISA), and affinity purification with protein A are also described.
Assuntos
Transfecção/métodos , Animais , Anticorpos/isolamento & purificação , Anticorpos/metabolismo , Técnicas de Cultura de Células , Proliferação de Células , DNA/genética , DNA/metabolismo , Ensaio de Imunoadsorção Enzimática , Células HEK293 , Humanos , Plasmídeos/genética , Plasmídeos/isolamento & purificação , Polietilenoimina/química , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/isolamento & purificação , Proteína Estafilocócica A/biossíntese , Proteína Estafilocócica A/isolamento & purificaçãoRESUMO
Three synthesis lots of linear poly(ethyleneimine) (PEI) are compared to a fully hydrolyzed linear PEI (commercially available as PEI "Max") regarding structure, polyplex formation with plasmid DNA, and transfection of suspension-adapted HEK-293E cells. PEI "Max" binds DNA more efficiently than the other PEIs, but it is the least effective in terms of transient recombinant protein yield. One PEI lot is fractionated by means of SEC. The fractions of high-M(n) PEI are the most efficient for complex formation and transfection. Nevertheless, the highest transient recombinant protein yields are achieved with unfractionated PEI. The results demonstrate that the polydispersity and charge density of linear PEI are important parameters for gene delivery to suspension-adapted HEK-293E cells.
Assuntos
DNA/química , Técnicas de Transferência de Genes , Vetores Genéticos/química , Plasmídeos/química , Polietilenoimina/química , Fracionamento Químico , DNA/genética , Expressão Gênica , Proteínas de Fluorescência Verde/biossíntese , Proteínas de Fluorescência Verde/genética , Células HEK293 , Humanos , Espectroscopia de Ressonância Magnética , Microscopia de Fluorescência , Peso Molecular , Plasmídeos/genética , Eletricidade Estática , TransfecçãoRESUMO
Here we describe a simplified method for transient gene expression (TGE) in suspension-adapted Chinese hamster ovary (CHO) cells using polyethylenimine (PEI) for DNA delivery. Both the transfection and production phases of the bioprocess were performed at a density of 4 × 106 cells/mL at 31 °C. In addition, the amounts of both PEI and plasmid DNA were reduced up to 50% on a per cell basis compared to previously published protocols from this laboratory, resulting in higher cell viability after transfection and higher volumetric recombinant protein yields. In batch cultures of up to 14 days, reproducible recombinant antibody yields up to 300 mg/L were achieved at small scale (5 mL) and up to 250 mg/L at large scale (500 mL). The simplicity and improved yields are expected to increase the utility of CHO cells for the rapid production of recombinant proteins at larger scales by TGE.
Assuntos
Biotecnologia/métodos , Expressão Gênica , Animais , Células CHO , Contagem de Células , Cricetinae , Cricetulus , DNA/genética , Plasmídeos/genética , Polietilenoimina/farmacologia , Proteínas Recombinantes/biossíntese , TransfecçãoRESUMO
Scaffold materials should favor cell attachment and proliferation, and provide designable 3D structures with appropriate mechanical strength. Collagen matrices have proven to be beneficial scaffolds for tissue regeneration. However, apart from small intestinal submucosa, they offer a limited mechanical strength even if crosslinking can enhance their mechanical properties. A more cell-friendly way to increase material strength is to combine synthetic polymer meshes with plastic compressed collagen gels. This work describes the potential of plastic compressed collagen-poly(lactic acid-co-É-caprolactone) (PLAC) hybrids as scaffolds for bladder tissue regeneration. Human bladder smooth muscle and urothelial cells were cultured on and inside collagen-PLAC hybrids in vitro. Scaffolds were analyzed by electron microscopy, histology, immunohistochemistry, and AlamarBlue assay. Both cell types proliferated in and on the hybrid, forming dense cell layers on top after two weeks. Furthermore, hybrids were implanted subcutaneously in the backs of nude mice. Host cell infiltration, scaffold degradation, and the presence of the seeded bladder cells were analyzed. Hybrids showed a lower inflammatory reaction in vivo than PLAC meshes alone, and first signs of polymer degradation were visible at six months. Collagen-PLAC hybrids have potential for bladder tissue regeneration, as they show efficient cell seeding, proliferation, and good mechanical properties.
Assuntos
Caproatos/química , Colágeno/química , Ácido Láctico/química , Lactonas/química , Polímeros/química , Engenharia Tecidual/métodos , Alicerces Teciduais/química , Bexiga Urinária/citologia , Animais , Células Cultivadas , Humanos , Imuno-Histoquímica , Camundongos , Microscopia de Força Atômica , PoliésteresAssuntos
Expressão Gênica/genética , Proteínas Recombinantes/genética , Proteínas Recombinantes/uso terapêutico , Transfecção/métodos , Animais , Fosfatos de Cálcio , Linhagem Celular , Humanos , Mamíferos , Plasmídeos , Polietilenoimina , Proteínas Recombinantes/metabolismo , Fatores de Tempo , Transfecção/instrumentação , Transformação Genética/genéticaRESUMO
Although respiratory syncytial virus (RSV) causes severe lower respiratory tract infection in infants and adults at risk, no RSV vaccine is currently available. In this report, efforts toward the generation of an RSV subunit vaccine using recombinant RSV fusion protein (rRSV-F) are described. The recombinant protein was produced by transient gene expression (TGE) in suspension-adapted human embryonic kidney cells (HEK-293E) in 4 L orbitally shaken bioreactors. It was then purified and formulated in immunostimulating reconstituted influenza virosomes (IRIVs). The candidate vaccine induced anti-RSV-F neutralizing antibodies in mice, and challenge studies in cotton rats are ongoing. If successful in preclinical and clinical trials, this will be the first recombinant subunit vaccine produced by large-scale TGE in mammalian cells.