RESUMO
Most food packages are made of plastics, nanoplastics released from which can be directly ingested and induce serious damage to organisms. Therefore, it is urgent to develop an effective and convenient method for nanoplastic determinations in food packages. In this work, we present a sandwich-based electrochemical strategy for nanoplastic determination. Positively charged Au nanoparticles were coated onto a Au electrode to selectively capture negatively charged nanoplastics in an aqueous environment. Subsequently, the nanoplastics were recognized by the signal molecule ferrocene via the hydrophobic interaction and determined by differential pulse voltammetry. Our sandwich-type detection depends on both electronegativity and hydrophobicity of nanoplastics, which make the method applicable for the assays of packages made of widely commercialized polystyrene (PS), polypropylene (PP), polyethylene (PE), and polyamide (PA). The method displays different sensitivities to above four nanoplastics but the same dynamic range from 1 to 100 µg·L-1. Based on it, the nanoplastics released from several typical food packages were assayed. Teabags were revealed with significant nanoplastic release, while instant noodle boxes, paper cups, and take-out boxes release slightly. The good recoveries in nanoplastic-spiked samples confirm the accuracy and applicability of this method. This work provides a sensitive, low-cost, and simple method without complicated instruments and pretreatment, which is of great significance for the determination of nanoplastics released from food packages.
Assuntos
Nanopartículas Metálicas , Poluentes Químicos da Água , Ouro , Interações Hidrofóbicas e Hidrofílicas , Metalocenos , Microplásticos , Nylons , Plásticos , Polietileno , Polipropilenos , Poliestirenos/química , Eletricidade Estática , Poluentes Químicos da Água/químicaRESUMO
Phospholipase D (PLD) is a critical component of intracellular signal transduction and has been implicated in many important biological processes. It has been observed that there are abnormalities in PLD expression in many human cancers, and PLD is thus recognized as a potential diagnostic biomarker as well as a target for drug discovery. We report for the first time a phospholipid-modified nanoprobe for ratiometric upconversion fluorescence (UCF) sensing and bioimaging of PLD activity. The nanoprobe can be synthesized by a facile one-step self-assembly of a phospholipid monolayer composed of poly(ethylene glycol) (PEG)ylated phospholipid and rhodamine B-labeled phospholipid on the surface of upconversion nanoparticles (UCNPs) NaYF4: 20%Yb, 2%Er. The fluorescence resonance energy transfer (FRET) process from the UCF emission at 540 nm of the UCNPs to the absorbance of the rhodamine B occurs in the nanoprobe. The PLD-mediated hydrolysis of the phosphodiester bond makes rhodamine B apart from the UCNP surface, leading to the inhibition of FRET. Using the unaffected UCF emission at 655 nm as an internal standard, the nanoprobe can be used for ratiometric UCF detection of PLD activity with high sensitivity and selectivity. The PLD activity in cell lysates is also determined by the nanoprobe, confirming that PLD activity in a breast cancer cell is at least 7-fold higher than in normal cell. Moreover, the nanoprobe has been successfully applied to monitoring PLD activity in living cells by UCF bioimaging. The results reveal that the nanoprobe provides a simple, sensitive, and robust platform for point-of-care diagnostics and drug screening in biomedical applications.
Assuntos
Transferência Ressonante de Energia de Fluorescência/métodos , Imagem Molecular/métodos , Nanopartículas/química , Fosfolipase D/análise , Fosfolipídeos/química , Linhagem Celular/enzimologia , Linhagem Celular Tumoral/enzimologia , Fluorescência , Humanos , Hidrólise , Imagem Molecular/instrumentação , Polietilenoglicóis/química , Rodaminas/química , Soluções , Itérbio/química , Ítrio/químicaRESUMO
Nanoplastics, widely existing in the environment and organisms, have been proven to cross the blood-brain barrier, increasing the incidence of neurodegenerative diseases like Alzheimer's disease (AD). However, current studies mainly focus on the neurotoxicity of nanoplastics themselves, neglecting their synergistic effects with other biomolecules and the resulting neurotoxicity. Amyloid ß peptide (Aß), which triggers neurotoxicity through its self-aggregation, is the paramount pathogenic protein in AD. Here, employing polystyrene nanoparticles (PS) as a model for nanoplastics, we reveal that 100 pM PS nanoparticles significantly accelerate the nucleation rate of two Aß subtypes (Aß40 and Aß42) at low concentrations, promoting the formation of more Aß oligomers and leading to evident neurotoxicity. The hydrophobic surface of PS facilitates the interaction of hydrophobic fragments between Aß monomers, responsible for the augmented neurotoxicity. This work provides consequential insights into the modulatory impact of low-dose PS on Aß aggregation and the ensuing neurotoxicity, presenting a valuable foundation for future research on the intricate interplay between environmental toxins and brain diseases.
Assuntos
Doença de Alzheimer , Nanopartículas , Humanos , Peptídeos beta-Amiloides/química , Peptídeos beta-Amiloides/metabolismo , Peptídeos beta-Amiloides/toxicidade , Microplásticos , Fragmentos de Peptídeos/química , Fragmentos de Peptídeos/metabolismo , Fragmentos de Peptídeos/toxicidadeRESUMO
Nanoparticles are usually used as carrier to load more antibody and enzyme to improve the sensitivity of enzyme-linked immunosorbent assay (ELISA). However, limited by high density and complicated modification procedure, the traditional nanoparticles such as Au nanoparticles (AuNPs) usually induce large background signal and poor reproducibility in ELISA. In this work, functional lightweigh nanoparticle polystyrene@polydopamine (PS@PDA) was prepared and induced as the carrier of detection antibody and horseradish peroxidase (HRP) to form PS@PDA@Ab2/HRP biojungates. The appropriate density (close to water) and good hydropilicity ensure the good dispersion of PS@PDA@Ab2/HRP in solution, preventing the physical sedimention and decreasing the background signal even though the bioconjugate's size is close to 200 nm. The large surface area and abundant active group from PDA facilitate the loading of detection antibody and HRP, improving the loading efficiency and stability of biojungates. Based on it, taking interleukin-17A (IL-17A, a biomarker of psoriasis) as the detection target, we developed a PS@PDA-based sandwich ELISA, achieving a sensitive dynamic range from 0.3 to 80 pg/mL and a detection limit of 0.2 pg/mL. Furthermore, the contents of IL-17A were assayed successfully in 10-fold diluted serum samples from psoriasis patients. Compared with those commercial or AuNP-based ELISA, our PS@PDA-based ELISA method exhibits higher sensitivity, lower background interference, and higher stability, which will significantly improve the application of ELISA in the low-abundant biomolecule assays.
Assuntos
Técnicas Biossensoriais , Nanopartículas Metálicas , Psoríase , Humanos , Ouro , Poliestirenos , Interleucina-17 , Reprodutibilidade dos Testes , Ensaio de Imunoadsorção Enzimática , Peroxidase do Rábano Silvestre , Anticorpos , Técnicas Biossensoriais/métodosRESUMO
Polydopamine (PDA) nanoparticles, as a kind of popular polymer material, have attracted a great deal of attention from various areas including materials science, biomedicine, energy, environmental science and so on owing to their striking physicochemical properties. Herein, we reported for the first time the synthesis of intrinsic fluorescent PDA nanoparticles using MnO2 as an oxidant. In the presence of MnO2, dopamine was quickly oxidized into its quinone derivative, and autopolymerized into fluorescent PDA nanoparticles. Using fluorescent PDA nanoparticles as a fluorescence signal indicator, we further established a cost-effective sensor for rapid, sensitive and selective sensing of reduced glutathione (GSH) based on the redox reaction between MnO2 and GSH, and the key role of MnO2 in the formation of fluorescent PDA nanoparticles. GSH has the capability of reducing MnO2 into Mn(2+), which inhibited the formation of the fluorescent PDA nanoparticles. Thus, the concentration of GSH was directly related to the decreased fluorescence signal intensity of the PDA nanoparticles. The sensor showed good sensing performance for GSH detection with high sensitivity and desirable selectivity over other potential interfering species. Additionally, the sensor exhibited excellent practical applications for GSH detection in human whole blood samples, which presents potential applications in biological detection and clinical diagnosis.
Assuntos
Glutationa/sangue , Indóis/química , Compostos de Manganês/química , Nanopartículas , Óxidos/química , Polímeros/química , HumanosRESUMO
Four types of straw, namely, soybean, wheat, corn, and rice, were investigated for use in lactic acid production. These straws were mainly composed of cellulose, hemicellulose, and lignin. After pretreatment with ammonia, the cellulose content increased, whereas the hemicellulose and lignin contents decreased. Analytical results also showed that the liquid enzymatic hydrolysates were primarily composed of glucose, xylose, and cellobiose. Preliminary experiments showed that a higher lactic acid concentration could be obtained from the wheat and soybean straw. However, soybean straw was chosen as the substrate for lactic acid production owing to its high protein content. The maximum lactic acid yield (0.8 g/g) and lactic acid productivity (0.61 g/(l/h)) were obtained with an initial reducing sugar concentration of 35 g/l at 30°C when using Lactobacillus casei (10% inoculum) for a 42 h fermentation period. Thus, the experimental results demonstrated the feasibility of using a soybean straw enzymatic hydrolysate as a substrate for lactic acid production.
Assuntos
Fermentação , Glycine max , Ácido Láctico/isolamento & purificação , Ácido Láctico/metabolismo , Lacticaseibacillus casei/metabolismo , Celobiose/metabolismo , Celulose/metabolismo , Glucose/metabolismo , Hidrólise , Lignina/química , Lignina/metabolismo , Oryza/química , Oryza/metabolismo , Glycine max/química , Glycine max/metabolismo , Triticum/química , Triticum/metabolismo , Difração de Raios X , Xilose/metabolismo , Zea mays/química , Zea mays/metabolismoRESUMO
Blood glucose monitoring has attracted extensive attention because diabetes mellitus is a worldwide public health problem. Here, we reported an upconversion fluorescence detection method based on manganese dioxide (MnO2)-nanosheet-modified upconversion nanoparticles (UCNPs) for rapid, sensitive detection of glucose levels in human serum and whole blood. In this strategy, MnO2 nanosheets on the UCNP surface serve as a quencher. UCNP fluorescence can make a recovery by the addition of H2O2, which can reduce MnO2 to Mn(2+), and the glucose can thus be monitored based on the enzymatic conversion of glucose by glucose oxidase to generate H2O2. Because of the nonautofluorescent assays offered by UCNPs, the developed method has been applied to monitor glucose levels in human serum and whole blood samples with satisfactory results. The proposed approach holds great potential for diabetes mellitus research and clinical diagnosis. Meanwhile, this nanosystem is also generalizable and can be easily expanded to the detection of various H2O2-involved analytes.
Assuntos
Glicemia/análise , Peróxido de Hidrogênio/análise , Compostos de Manganês/química , Nanopartículas Metálicas/química , Óxidos/química , Espectrometria de Fluorescência/métodos , Glucose Oxidase/química , Teste de Materiais , Membranas Artificiais , Nanopartículas Metálicas/ultraestrutura , Oxigenases de Função Mista/química , Tamanho da Partícula , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
Correlation between fractional, biodegradable and spectral characteristics of sludge extracellular polymeric substances (EPS) by different protocols has not been well established. This work extracted sludge EPS using alkaline extractants (NH4OH and formaldehyde + NaOH) and physical protocols (ultrasonication, heating at 80 °C or cation exchange resin (CER)) and then fractionated the extracts using XAD-8/XAD-4 resins. The alkaline extractants yielded more sludge EPS than the physical protocols. However, the physical protocols extracted principally the hydrophilic components which were readily biodegradable by microorganisms. The alkaline extractants dissolved additional humic-like substances from sludge solids which were refractory in nature. Different extraction protocols preferably extracted EPS with distinct fractional, biodegradable and spectral characteristics which could be applied in specific usages.
Assuntos
Biopolímeros/isolamento & purificação , Fracionamento Químico/métodos , Espaço Extracelular/química , Compostos Orgânicos/isolamento & purificação , Esgotos/química , Absorção/efeitos da radiação , Biodegradação Ambiental , Carboidratos/análise , Oxigênio/análise , Proteínas/análise , Solubilidade/efeitos da radiação , Espectrometria de Fluorescência , Espectroscopia de Infravermelho com Transformada de Fourier , Raios UltravioletaRESUMO
The oral and maxillofacial cutaneous or/and oral mucous wound of 20 cases were sutured with BD-018 medical natural absorbable surgical suture. Fifteen cases with class I wound and five cases with class II wound healed well after operation. BD-018 medical natural absorbable surgical suture adapts to oral and maxillofacial cutaneous or/and oral mucous wound suturing.
Assuntos
Cirurgia Bucal/métodos , Suturas , Adolescente , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , CicatrizaçãoRESUMO
Silica nanoparticles were prepared in a microemulsion system, using polyoxyethylene nonylphenyl ether/cyclohexane/ammonium hydroxide. The surface charge of the particle was modified with PLL [poly(L-lysine)]. PAGE demonstrated the ability of PMS-NP (PLL-modified silica nanoparticles) to bind and protect antisense ODNs (oligonucleotides). The intracellular localization of FITC-labelled ODN was investigated by fluorescence microscopy. The results demonstrated that ODN could be delivered to cytoplasm. Flow-cytometry analysis showed a 20-fold enhancement of ODN delivered by PMS-NP compared with free ODN for a serum-free medium. Blocking efficacy of c- myc antisense ODN, delivered by PMS-NP, was examined in HNE1 and HeLa cell lines. Significant down-regulation of c- myc mRNA levels was observed in both the cell lines. However, the cellular uptake efficiency and antisense effects on target gene decreased in the presence of serum-containing medium. The analysis of the filtration assay showed that PMS-NP interacted with serum proteins. These results indicated that PMS-NP was a suitable delivery vector for antisense ODN, although its delivery efficiency decreased in the presence of a serum-containing medium.