RESUMO
In this study, Fe3O4/polyacrylonitrile (PAN) magnetic nanofibers (MNFs) were fabricated by electrospinning method to immobilize the horseradish peroxidase (HRP), making which a complex platform for phenol removal application. Results indicated that, the average diameter of MNFs was about 200-400â¯nm and the maximum saturation magnetic induction was 19.03â¯emu/g. Compared with the free HRP, the modified HRP showed no change in optimum pH, but showed higher catalytic activity. Moreover, HRP immobilized MNFs (H-MNFs) with 40% Fe3O4 nanoparticles loading had the lowest HRP loading, but had the highest relative activity, because of the magnetic synergy with the presence of MNPs. Subsequently, the 40% H-MNFs was used for the remediation of phenol wastewater, achieved the removal efficiency of phenol to 85% in the first round use, and remained 52% of efficiency after 5 recycles using. It was expected that the H-MNFs could be a potential application in wastewater treatment such as phenol removal.