Bioaccumulation of Methyl Siloxanes in Common Carp (Cyprinus carpio) and in an Estuarine Food Web in Northeastern China.

Despite the high production volume and widespread use of methyl siloxanes, limited studies have been conducted to investigate the bioconcentration, biomagnification, and trophic magnification potentials of these substances. In the present study, bioconcentration factors (BCFs) of methyl siloxanes were determined with common carp exposed at environmental relevant levels for 32 days. BCF of octamethylcyclotetrasiloxane (D4) was estimated as 6197 L/Kg, indicating strong bioconcentration potential in the common carp. To assess the food chain transfer of methyl siloxanes, 12 aquatic invertebrates and vertebrates species were collected from a food web in Shuangtaizi estuary in northeastern China and concentrations of methyl siloxanes in these species were determined with gas chromatography mass spectrometry. Trophic magnification factors (TMFs) of decamethylcyclopentasiloxane (D5), dodecamethylcyclohexasiloxane (D6), and linear siloxanes (L7-10) were significantly greater than 1 in one food chain selected, which suggest trophic magnification potentials of these methyl siloxanes. Biomagnification factors of D4-D6 and L7-L10 from planktons to Japanese snapping shrimp were greater than 1, indicating biomagnification potentials of these methyl siloxanes from the prey to predator. This is the first study to investigate the bioaccumulation behaviors of methyl siloxanes by coupling BCF and BMF with TMF.

Resin-based dental sealants as a source of human exposure to bisphenol analogues, bisphenol A diglycidyl ether, and its derivatives.

Although studies have examined leaching of bisphenol A (BPA) from dental sealants into saliva, occurrence of BPA, bisphenol A diglycidyl ether (BADGE), and their derivatives in dental sealants themselves has not been investigated. In this study, concentrations of eight bisphenol analogues (BPs), BADGE and its derivatives (BADGEs), including BADGE‧H2O, BADGE‧HCl, BADGE‧2H2O, BADGE‧2HCl, and BADGE‧H2O‧HCl, were determined in 70 dental sealants collected from the U.S. market. Of the 70 dental sealants analyzed, 65 contained at least one of the target chemicals measured. BADGE‧2H2O was the most abundant compound, found at concentrations of up to 1780µg/g. The geometric mean (GM) concentration of total BADGEs was 47.8µg/g, which was two to three orders of magnitude higher than that of total BPs (GM: 539ng/g). BPA was found in 46% of the sealants and BADGEs was found in 87% of the sealants analyzed. Majority of the dental sealants analyzed in this study were manufactured in the United States and Korea; no significant differences were observed in the concentrations of BPs and BADGEs between the two countries. An exposure assessment was made based on the concentrations of BPs and BADGEs measured in sealants and their application rates in dentistry. The worst-case exposure scenario with the highest measured concentration of total BPs and BADGEs and application on 8 teeth at 8mg each yielded an estimated daily intake (EDI) of 1670 and 5850ng/kg·bw/day for adults and children, respectively. Although the EDI is below the specific migration limit set by the European Food Safety Authority, dental sealants are a source of exposure to BPs and BADGEs, especially in children.

Synthetic phenolic antioxidants, including butylated hydroxytoluene (BHT), in resin-based dental sealants.

Resin-based dental sealants (also referred to as pit-and-fissure sealants) have been studied for their contribution to bisphenol A (BPA) exposure in children. Nevertheless, little attention has been paid to the occurrence of other potentially toxic chemicals in dental sealants. In this study, the occurrence of six synthetic phenolic antioxidants (SPAs), including 2,6-di-tert-butyl-4-hydroxytoluene (BHT), 2,6-di-tert-butyl-4-(hydroxyethyl)phenol (BHT-OH), 3,5-di-tert-butyl-4-hydroxy-benzaldehyde (BHT-CHO), 2,6-di-tert-butylcyclohexa-2,5-diene-1,4-dione (BHT-Q), 3,5-di-tert-butyl-4-hydroxybenzoic acid (BHT-COOH) and 2-tert-butyl-4-methoxyphenol (BHA), was examined in 63 dental sealant products purchased from the U.S. market. BHT was found in all dental sealants at median and maximum concentrations of 56.8 and 1020µg/g, respectively. The metabolites of BHT and BHA were detected in 39-67% of samples, at concentration ranges of

Mechanisms of polystyrene microplastic degradation by the microbially driven Fenton reaction.

Natural hydroxyl radical (·OH) production, which partially occurs through the microbially driven Fenton reaction, can enhance the degradation of polystyrene microplastics (PS-MPs). However, ·OH causes damage to microorganisms, which might in turn restrain the microbially driven Fenton reaction. Thus, whether PS-MPs can be continuously degraded by the microbially driven Fenton reaction and how they are degraded are still unknown. A pure-culture system using Shewanella putrefaciens 200 was set up to explore the effect and mechanism of microbially driven Fenton reaction on PS-MP degradation. In a 14-day operation, ·OH produced by the microbially driven Fenton reaction could degrade PS-MPs with a weight loss of 6.1 ± 0.6% and an O/C ratio of 0.6 (v.s. 0.6 ± 0.1% and 0.1, respectively, in the ·OH quenched group). Benzene ring derivatives such as 2-isopropyl-5-methyl-1-heptanol and nonahexacontanoic acid were the main soluble products of PS-MP degradation. The ·OH-induced oxidative damage on microorganisms did not affect ·OH production significantly when there was timely replenishment of organic carbon sources to promote reproduction of microorganisms. Thus, PS-MPs can be continuously degraded by microbially driven Fenton reactions in natural alternating anaerobic-aerobic environments. This study also provides a new microbial technique for MP degradation that is different from previous technologies based on microbial plastic-degrading enzymes.