RESUMO
To investigate whether polymerized human placenta hemoglobin (PolyPHb) improved hemodynamic parameter recovery and cardiac function after hemorrhagic shock, a mean arterial pressure (MAP) of 30 mmHg was maintained for 60min. Then, all the rats were randomly resuscitated with hetastarch (HES), shed blood (Whole Blood), or PolyPHb (PolyPHb). The MAP was greatly improved by PolyPHb, but it was still lower than that of the Whole Blood group. Meanwhile, the cardiac function and enzyme releases in the PolyPHb group were similar to the HES group. Therefore, our findings suggest that PolyPHb moderately improved hemodynamic recovery and provided little cardioprotective effect in hemorrhagic shock.
Assuntos
Substitutos Sanguíneos/farmacologia , Coração/efeitos dos fármacos , Coração/fisiopatologia , Hemodinâmica/efeitos dos fármacos , Hemoglobinas/farmacologia , Placenta/química , Choque Hemorrágico/fisiopatologia , Animais , Biopolímeros/farmacologia , Pressão Sanguínea/efeitos dos fármacos , Modelos Animais de Doenças , Feminino , Humanos , Masculino , Perfusão , Gravidez , Ratos , Ratos Sprague-Dawley , Recuperação de Função Fisiológica/efeitos dos fármacosRESUMO
A new procedure was developed to obtain high-quality polymerized human hemoglobin by modifying purified hemoglobin with PLP and polymerized with GDA. Comparing polymerized hemoglobin products obtained from different methods, the product from the new procedure has similar physical, chemical, and biological properties in the molecular distribution, methemoglobin concentration, oxygen carrier capacity, P(50) and spectral analysis. Furthermore, the new procedure of modification after polymerization can save PLP greatly, and significantly reduce the cost. So the procedure of modification after polymerization is a better way in research and production.