New Insights into the Role of Oral Microbiota Dysbiosis in the Pathogenesis of Inflammatory Bowel Disease.

Inflammatory bowel disease (IBD) is a group of chronic intestinal inflammatory disorders with a prolonged duration characterized by recurrent relapse and remission. The exact etiology of IBD remains poorly understood despite the identification of relevant risk factors, including individual genetic susceptibility, environmental triggers, and disruption of immune homeostasis. Dysbiosis of the gut microbiota is believed to exacerbate the progression of IBD. Recently, increasing evidence has also linked oral microbiota dysbiosis with the development of IBD. On the one hand, IBD patients show significantly unbalanced composition and function of the oral microbiota known as dysbiosis. On the other, overabundances of oral commensal bacteria with opportunistic pathogenicity have been found in the gut microbiota of IBD patients. Herein, we review the current information on the causative factors of IBD, especially recent evidence of IBD-associated oral microbiota dysbiosis, which has seldom been covered in the previous literature review, highlighting the pathogenic mechanisms of specific oral bacteria in the development of IBD. Ectopic colonization of several oral bacteria, including a subset of Porphyromonas gingivalis, Streptococcus mutans, Fusobacterium nucleatum, Campylobacter concisus, and Klebsiella pneumoniae, may lead to destruction of the intestinal epithelial barrier, excessive secretion of inflammatory cytokines, disruption of the host immune system, and dysbiosis of gut microbiota, consequently aggravating chronic intestinal inflammation. Studying oral microbiota dysbiosis may open future horizons for understanding IBD pathogenesis and provide novel biomarkers for IBD. This review also presents the current treatment and new perspectives for IBD treatment.

Treatment of Atherosclerotic Femoropopliteal Artery Disease with Supera Interwoven Nitinol Stent: A Real-World Study in China.

BACKGROUND: To analyze the outcomes of Supera stent deployment in Chinese patients with atherosclerotic femoropopliteal artery (FPA) disease in a real-world setting. METHODS: This retrospective cohort study collected and analyzed the medical records of 246 consecutive patients who received Supera stents for FPA disease at the China Academy of Chinese Medical Sciences Xiyuan Hospital between February 2017 and December 2019. All study patients underwent balloon angioplasty and were treated with Supera stents (Abbott Vascular, Santa Clara, CA, USA). The primary outcome was the rate of primary patency 12 months after discharge. RESULTS: The analyses included 246 consecutive patients and 260 lesions. The mean ± SD age was 73.2 ± 9.9 years and most patients (60.2%) were males. Of the 260 treated lesions, Supera stents were deployed in eight (3.1%) cases after a previous stent fracture. Critical limb ischemia was diagnosed in 87.3% of the limbs, and 84 (32.3%) and 83 (31.5%) cases were classified as TransAtlantic Inter-Society Consensus (TASC) C and D, respectively. Most of the lesions were in situ (80.8%) and located in the superficial femoral artery (45.0%) or the FPA (45.8%). The mean lesion length was 147.7 mm. Nominal deployment (-10 to 10% compression) was the most common deployment scenario (84.1%). The 1-year primary patency rate was 80.6%. Lesions that occurred as restenosis (odds ratio [OR]: = 3.34, 95% confidence interval [CI]: 1.03-10.85, P = 0.045) or in-stent restenosis (OR: = 2.88, 95% CI: 1.03-8.07, P = 0.045) were independently associated with occlusion or stenosis after stent deployment. No stent fracture was observed in this study. CONCLUSIONS: Our study indicates that the use of Supera stents is feasible for the treatment of Chinese patients with FPA disease. The long-term results reveal high primary patency.

Rational Design of Stimuli-Responsive Polymers Modified Nanopores for Selective and Sensitive Determination of Salivary Glucose.

The great pain and stress from finger-prick glucose measurements have resulted in great motivation to find noninvasive glucose monitoring technologies where salivary glucose measurement is desirable. However, the relative low concentration of glucose and coexisting chemicals in saliva challenges the sensitive and selective salivary glucose detection. In this article, we have rationally designed and constructed a salivary glucose sensor by modifying the inner wall of the Au-decorated glass nanopore with stimuli-responsive copolymer poly(3-(acryloylthioureido) phenylboronic acid-co-N-isopropylacrylamide) (denoted as PATPBA-co-PNIPAAm) via Au-S interaction. Notably, upon recognition of glucose, the copolymer could undergo a wettability switch and pKa shifts in the boronic acid functional groups, which significantly regulated the ion transport through nanopores, thus showing improved sensitivity with the detection limit of 1 nM. Moreover, benefiting from the multivalent boronic acid-glucose interaction and the cooperation of thiourea units, the copolymer exhibited good selectivity for glucose detection against the coexisting saccharides and other biological molecules in saliva. The nanopores with well-demonstrated analytical performance were finally applied for monitoring glucose in saliva. Together, this work unveiled a new platform for glucose detection in saliva, and promised to provide a new strategy for detecting other biomolecules in accessible biofluid involved in physiological and pathological events.

Eco-Friendly Ca-Montmorillonite Grafted by Non-Acidic Ionic Liquid Used as A Solid Acid Catalyst in Cellulose Hydrolysis to Reducing Sugars.

An effective and friendly method was developed for the production of reducing sugars (RS) from the hydrolysis of cellulose over the solid catalyst of Ca-montmorillonite (Mt) grafted by non-acidic ionic liquid (Mt-IL) in water. The effect of mass ratio, water dosage, reaction temperature, and time were investigated in a batch reactor. Raw Mt showed only a 7.9% total reducing sugars (TRS) yield for the catalytic hydrolysis of cellulose in water. As the Mt was grafted by -SO3H and IL, the TRS yield greatly increased under the same reaction conditions. The highest TRS yield of 35.7% was obtained on the catalyst of Mt grafted by non-acidic IL at 200 °C with the mass ratio of catalyst to cellulose of 0.2 for 120 min. The high TRS yield for Mt-IL should be attributed to the synergistic effect of the dissolution of cellulose by IL and the exposed metal ions on the layer with water. Although the yield of TRS on Mt-IL decreased gradually with recycling runs, the decrease after the first run was not very serious compared to the fresh catalyst. This work provides a promising strategy for efficient cellulose hydrolysis into fine chemicals by Mt with non-acidic IL.

Role of light microplastics in the dispersion process of spilled crude oil in the marine environment.

Oil spill and microplastic (MP) pollution are the main problems in the marine environment. After an oil spill, the oil film may be dispersed into the water column in the form of droplets under the action of ocean waves. In this study, the sea condition was simulated through the batch conical flask oscillation experiment. Merey crude oil was selected as experimental oil, and polyethylene (PE) and polystyrene (PS) were used as experimental MP. The effects of MP properties (type, concentration and size) on the dispersion of spilled oil were investigated. It is found that for each MP, the oil dispersion efficiency (ODE) increased rapidly at first and then tended to be stable, which all reached the maximum at 360 min. When the concentrations of PE and PS increased from 0 to 100 mg/L, the maximum ODE decreased from 32.64 % to 13.72 % and 10.75 %, respectively, indicating that the presence of MP inhibits the oil dispersion. At the same oscillation time, the volumetric mean diameter (VMD) of dispersed oil increased with the MP concentration. When the particle size of PE and PS increased from 13 to 1000 µm, the maximum ODE increased from 24.74 % to 31.49 % and 28.60 %, respectively. However, the VMD decreased with the size of MP. In addition, the time series of the oil adsorption rate by the MP were well fitted by the kinetic models. The results of this research deepen the understanding of the migration law of spilled oil to the marine environment in the presence of MP, and may further improve the ability of marine environmental scientists to predict the fate of oil spill.

Determination of ATP using a double-receptor sandwich method based on molecularly imprinted membrane and fluorescence-labeled uranyl-salophen complex.

A double-receptor sandwich method for the fluorescence determination of adenosine triphosphate (ATP) is proposed in this paper. The solid phase receptor on the surface of glass slides is a molecularly imprinted membrane (MIM) containing an artificial nanocavity. It is constructed by a molecular imprinting technique using adenosine monophosphate (AMP) as a template molecule. The labeled receptor is a uranyl-salophen complex containing a fluorescent group or uranyl-salophen-fluorescein (USF). It is synthesized with salophen, 5-aminofluorescein, and uranyl. In a procedure of determining ATP, ATP in sample solution is first adsorbed on the surface of the glass slide through the combination of the AMP group in ATP with the nanocavity in MIM. Then, the adsorbed ATP binds USF through the coordination reaction of the phosphate group in ATP with uranyl in USF to form a sandwich-type structure of MIM-ATP-USF. The amount of ATP is detected through the fluorescence determination of USF bound on the slide. Under optimal conditions, the linear range for the determination of ATP is 0.3 to 4.8 nmol/mL with a detection limit of 0.041 nmol/mL. The proposed method has been successfully employed for the determination of ATP in real samples with the recoveries of 98.5 to 102.5 %.

Impact of mixing energy and dispersant dosage on oil dispersion and sedimentation with microplastics in the marine environment.

Recently, the fate of spilled oil in the presence of microplastics (MPs) in the sea has attracted attention of researchers. Merey crude oil and polyethylene terephthalate (PET) were used as the experimental materials in this study. The effects of mixing energy and dispersant dosage on oil dispersion and sedimentation in the presence of MPs in the water column were investigated by laboratory experiments simulating actual sea conditions. The increase of mixing energy showed a promoting effect on oil dispersion. When the oscillation frequency increased from 140 rpm to 180 rpm, the oil dispersion efficiency (ODE) ranged from 2.1 %-3.7 % to 17.4 %-30.8 %, and the volumetric mean diameter (VMD) of the suspended oil droplets/MPs-oil agglomerates (MOA) decreased from 99.9-131.4 µm to 76.6-88.2 µm after 2 h oscillation. The application of chemical dispersant led to an increase in both the quantity and size of the formed sunken MPs-oil-dispersant agglomerates (MODA). At the dispersant-to-oil ratio (DOR) of 1:5, the ODE declined from 77.7 % to 62.6 % when the MPs concentration increased from 0 to 150 mg/L, while the oil sinking efficiency (OSE) rose from 3.4 % to 15.6 % when the MPs increased from 25 to 150 mg/L; the maximum size of the sunken MODA reached 13.0 mm, and the total volume of the MODA formed per unit volume oil reached 389.7 µL/mL oil at the MPs concentration of 150 mg/L. Meanwhile, the results showed that the presence of MPs inhibited the oil dispersion by increasing the oil-water interfacial tension. The outcomes of this work may provide assistance in predicting the transport of spilled oil and developing emergency measures.

Multiple H-bonds directed self-assembly of an amphiphilic and plate-like codendrimer with janus faces at water-air interface.

An amphiphilic diblock codendrimer composed of a third generation poly(methallyl dichloride) end-capped by eight hydroxyl groups (PMDC(OH)(8)) and a second generation poly(urethane amide) end-capped by four alkyl groups (PUA(C16)(4)) were found to self-assemble into highly oriented ribbons at the water-air interface. Further investigation on the ribbon formation shows that the ribbons are hierarchically self-organized by the janus and plate-like shape of g3-PMDC(OH)(8)-b-g2-PUA(C16)(4). Sextuple H-bonds existing at different positions of the molecular plate are the main driving force for the one-dimensional growth of the ribbon. The recognition of these H-bonds leads to a highly ordered stacking of the codendrimers, and the crystallization of the alkyl chains results in a primary ribbon with a ca. 7.6 +/- 0.5 nm width. The primary ribbons prefer to organize into secondary ribbons with an average width of 53 +/- 6.0 nm. The manner of recognition and assembly is similar to the organization of a kind of toy building block with janus faces, which provides a new strategy to the design of well-defined nanomaterials.

[Clinical analysis of maxillofacial patients with diabetes on perioperative period].

OBJECTIVE: To investigate the treatment of perioperative period on maxillofacial patients with diabetes. METHODS: The retrospective analysis was taken on clinical data of 24 cases of maxillofacial patients with diabetes. RESULTS: All patients recovered without severe complications by controlling blood sugar during whole stage and preventing infection with antibiotics after surgery. The wounds in 21 cases healed in 10-14 days after the operations and that didn't in 3 cases. With the treatment on local wounds, the wounds in 3 cases healed in 14-28 days after the operations. CONCLUSION: It's the treatment principle of maxillofacial surgical patients with diabetes to monitor the levels of blood glucose on time, to control the levels of blood glucose rigorously at the whole stage and to prevent complication.