A regulatory mechanism of a stepwise osteogenesis-mimicking decellularized extracellular matrix on the osteogenic differentiation of bone marrow-derived mesenchymal stem cells.

A cell-derived decellularized extracellular matrix (dECM) plays a vital role in controlling cell functions because of its similarity to the in vivo microenvironment. In the process of stem cell differentiation, the composition of the dECM is not constant but is dynamically remolded. However, there is little information regarding the dynamic regulation by the dECM of the osteogenic differentiation of stem cells. Herein, four types of stepwise dECMs (0, 7, 14, and 21 d-ECM) were prepared from bone marrow-derived mesenchymal stem cells (BMSCs) undergoing osteogenic differentiation for 0, 7, 14, and 21 days after decellularization. In vitro experiments were designed to study the regulation of BMSC osteogenesis by dECMs. The results showed that all the dECMs could support the activity and proliferation of BMSCs but had different effects on their osteogenic differentiation. The 14d-ECM promoted the osteogenesis of BMSCs significantly compared with the other dECMs. Proteomic analysis demonstrated that the composition of dECMs changed over time. The 14d ECM had higher amounts of collagen type IV alpha 2 chain (COL4A2) than the other dECMs. Furthermore, COL4A2 was obviously enriched in the activated focal adhesion kinase (FAK)/phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)/protein kinase B (AKT) signaling pathways. Thus, the 14d-ECM could promote the osteogenic differentiation of BMSCs, which might be related to the high content of COL4A2 in the 14d-ECM by activating the FAK/PI3K/AKT signaling pathways.

Effect of Access Cavities and Canal Enlargement on Biomechanics of Endodontically Treated Teeth: A Finite Element Analysis.

INTRODUCTION: The purpose of this study was to investigate the influence of access cavities and tapers of canal preparations on fracture resistance of endodontically treated first molars by finite element method and Weibull analysis. METHODS: On the basis of the micro-computed tomography data of maxillary first molar, the models of endodontically treated teeth with conservative endodontic cavity, traditional endodontic cavity, and 4 tapers of canal preparations (0.02, 0.04, 0.06, and 0.08) were created. Four static loads (800 N in total) were applied vertically to the contact points. The stress distributions of maximum principal stress were recorded and analyzed. Weibull analysis was performed to analyze the failure risk in enamel and dentin. RESULTS: The stress distributions of maximum principal stress on occlusal surfaces were similar. In cervical region, the tensile stress was mainly concentrated on mesiobuccal root and root furcation. The finite element analysis and Weibull analysis showed that conservative endodontic cavity significantly reduced the maximum principal stress in cervical region and the failure probability, compared with traditional endodontic cavity. No significant difference was detected among tapers of prepared canals. CONCLUSIONS: Preserving coronal dentin by using conservative endodontic cavity significantly reduced the concentration of tensile stress and the failure probability of dentin, although the maximum principal stress and failure probability were less affected by taper of canal preparation.