Surface modification of solid-state nanopores for sticky-free translocation of single-stranded DNA.

Nanopore technology is one of the most promising approaches for fast and low-cost DNA sequencing application. Single-stranded DNA detection is primary objective in such device, while solid-state nanopores remain less explored than their biological counterparts due to bio-molecule clogging issue caused by surface interaction between DNA and nanopore wall. By surface coating a layer of polyethylene glycol (PEG), solid-state nanopore can achieve long lifetime for single-stranded DNA sticky-free translocation at pH 11.5. Associated with elimination of non-specific binding of molecule, PEG coated nanopore presents new surface characteristic as less hydrophility, lower 1/f noise, and passivated surface charge responsiveness on pH. Meanwhile, conductance blockage of single-stranded DNA is found to be deeper than double-stranded DNA, which can be well described by a string of blobs model for a quasi-equilibrium state polymer in constraint space.

Pressure-controlled motion of single polymers through solid-state nanopores.

Voltage-biased solid-state nanopores are well established in their ability to detect and characterize single polymers, such as DNA, in electrolytes. The addition of a pressure gradient across the nanopore yields a second molecular driving force that provides new freedom for studying molecules in nanopores. In this work, we show that opposing pressure and voltage bias enables nanopores to detect and resolve very short DNA molecules, as well as to detect near-neutral polymers.

Novel planar-structure electrochemical devices for highly flexible semitransparent power generation/storage sources.

Flexible and transparent power sources are highly desirable in realizing next-generation all-in-one bendable, implantable, and wearable electronic systems. The developed power sources are either flexible but opaque or semitransparent but lack of flexibility. Therefore, there is increasing recognition of the need for a new concept of electrochemical device structure design that allows both high flexibility and transparency. In this paper, we present a new concept for electrochemical device design--a two-dimensional planar comb-teeth architecture on PET substrate--to achieve both high mechanical flexibility and light transparency. Two types of prototypes--dye-sensitized solar cells and supercapacitors--have been fabricated as planar devices and demonstrated excellent device performance, such as good light transparency, excellent flexibility, outstanding multiple large bending tolerance, light weight, effective prevention of short circuits during bending, and high device integration with up-date microelectronics, compared to conventional sandwich structure devices. Our planar design provides an attractive strategy toward the development of flexible, semitransparent electrochemical devices for fully all-in-one elegant and wearable energy management.

Intrinsic and membrane-facilitated α-synuclein oligomerization revealed by label-free detection through solid-state nanopores.

α-Synuclein (α-Syn) is an abundant cytosolic protein involved in the release of neurotransmitters in presynaptic terminal and its aberrant aggregation is found to be associated with Parkinson's disease. Recent study suggests that the oligomers formed at the initial oligomerization stage may be the root cause of cytotoxicity. While characterizing this stage is challenging due to the inherent difficulties in studying heterogeneous and transient systems by conventional biochemical technology. Here we use solid-state nanopores to study the time-dependent kinetics of α-Syn oligomerization through a label-free and single molecule approach. A tween 20 coating method is developed to inhibit non-specific adsorption between α-Syn and nanopore surface to ensure successful and continuous detection of α-Syn translocation. We identify four types of oligomers formed in oligomerization stage and find an underlying consumption mechanism that the formation of large oligomers consumes small oligomers. Furthermore, the effect of lipid membrane on oligomerization of α-Syn is also investigated and the results show that 1,2-dioleoyl-sn-glycero-3-[phospho-L-serine] (DOPS) small unilamellar vesicles (SUVs) dramatically enhances the aggregation rate of α-Syn while do not alter the aggregation pathway.

Ultrafine and smooth full metal nanostructures for plasmonics.

Full metal various nanostructures with extremely smooth surface, small feature size and high aspect ratio can be fabricated by a template stripping method with EBL-patterned PMMA layer as a template. It is further demonstrated that our method is crucial to obtain clear and abundant plasmonic modes in nanocavities with metal reflectors.

Fabrication of polydiacetylene nanowires by associated self-polymerization and self-assembly processes for efficient field emission properties.

The paper described here concerns a challenge of general interest for producing a novel structure of a polymer aggregate, the achievement of nanowires with controlled diameters. We provide a strategy for fabricating a supramolecular polymer, in which ordered polydiacetylene nanowires can be obtained by associated self-polymerization and self-assembly processes. The polymer nanowire film shows excellent field emission properties with the turn-on field of 8.2 V/mum at 10 muA/cm2 and the maximum current density of 5 mA/cm2 at an applied field of 15 V/mum.