[Technique of macroporous resin for tartary buckwheat total flavonoids].

OBJECTIVE: To investigatre appropriate macroporous resins and the optimal technological parameters of the purification process of tartary buckwheat total flavonoids. METHOD: Static and dynamic adsorption-desorption methods were adopted, and evaluated for separating efficiency by macroporous resins absorption rates, desorption rates. RESULT: The DM -2 macroporous resin had the best separating efficiency. The best absorbed condition is H2O as solvent of extracts, diameter vs height: 1: 10, pH 3 - 4, absorption power: 3.0 mL x min(-1). The optimum adsorption condition is the volume of 50% ethanol 80 mL (approximately 5 BV (resin bed volume) as eluting solvent, pH 8, the desorption power: 3.0 mL x min(-1). CONCLUSION: This technology is simple, quick and cost-effective, which is suitable for industrialization.

Augmentation of screw fixation with injectable calcium sulfate bone cement in ovariectomized rats.

The objective of this study was to determine the effect of augmenting screw fixation with an injectable calcium sulfate cement (CSC) in the osteoporotic bone of ovariectomized rats. The influence of the calcium sulfate (CS) on bone remodeling and screw anchorage in osteoporotic cancellous bone was systematically investigated using histomorphometric and biomechanical analyses. The femoral condyles of 55 Sprague-Dawley ovariectomized rats were implanted with screw augmented with CS, while the contralateral limb received a nonaugmented screw. At time intervals of 2, 4, 8, 12, and 16 weeks, 11 rats were euthanized. Six pair-matched samples were used for histological analysis, while five pair-matched samples were preserved for biomechanical testing. Histomorphometric data showed that CS augmented screws activated cancellous bone formation, evidenced by a statistically higher (p < 0.05) percentage of osteoid surface at 2, 4, and 8 weeks and a higher rate of bone mineral apposition at 12 weeks compared with nonaugmented screws. The amount of the bone-screw contact at 2, 8, and 12 weeks and of bone ingrowth on the threads at 4 and 8 weeks was greater in the CS group than in the nonaugmented group (p < 0.05), although these parameters increased concomitantly with time for both groups. The CS was resorbed completely at 8 weeks without stimulating fibrous encapsulation on the screw surface. Also, the cement significantly increased the screw pull-out force and the energy to failure at 2, 4, 8, and 12 weeks after implantation, when compared with the control group (p < 0.05). These results imply that augmentation of screw fixation with CS may have the potential to decrease the risk of implant failure in osteoporotic bone.