Microplastics as potential carriers of viruses could prolong virus survival and infectivity.

Microplastics are emerging contaminants in various aquatic environments, leading to human and environmental health concerns. Viruses have also been ubiquitously detected in aquatic environments, and there is an unknown risk of microplastics-mediated virus migration through adsorption. This study applied polystyrene microplastics as the carrier and the T4 bacteriophage (or phage) as the virus model, and a violet side scatter/green fluorescence double-gated flow cytometry approach to investigate the adsorption capacity of viruses on microplastics. Our results show that up to 98.6±0.2% of the dosed viruses can be adsorbed by microplastics, and such adsorptions are dependent on size and surface functional groups. Both Fourier-transform infrared spectroscopy and fluorescence-labelled confocal microscopy confirmed that the virus can successfully adsorb onto microplastics. Zeta potential characterisation revealed that the electrostatic interaction is the primary adsorption mechanism associated with the adsorption of viruses. UV-aging was found to enhance the adsorption capacities of viruses on microplastics. Both pristine and UV-aged microplastics were found to significantly prolong the infectivity of the adsorbed viruses, even under elevated temperatures. Collectively, our findings highlight that microplastics are associated with the biological risks of water-borne viral transmission through virus adsorption.

Lignocellulosic residue as bio-carrier for algal biofilm growth: Effects of carrier physicochemical proprieties and toxicity on algal biomass production and composition.

Five types of lignocellulosic materials were applied as the bio-carriers for low-cost algal biofilm cultivation of three algal strains. The effects of bio-carrier physicochemical properties and toxicity on algal cells growth and attachment were investigated. Rougher and hydrophilic bio-carrier could yield more algal biomass than smoother and hydrophobic bio-carrier. Pine sawdust (diameter: 0.420-0.595 mm) performed the best when cultured Diplosphaera sp. (9.61 g·m-2·day-1) biofilm. Meanwhile, bio-carriers could be leached by the culture medium during cultivation, and their energy conversion proprieties could be improved due to the reduced ash contents and the decreased crystallinities. In addition, Chlorella vulgaris growth tests indicated that pine sawdust (15.45%) leachate promoted cell growth, whereas rick husk (15.48%) and sugarcane bagasse (13.19%) leachate inhibited cell growth. And bio-carriers leachates also modified the chemical compositions (lipid, protein and carbohydrate) of algal cells and increased the corresponding saturated fatty acids methyl ester content (from 48.71 to 55.58-57.08%).

A reagentless and reusable electrochemical aptamer-based sensor for rapid detection of ampicillin in complex samples.

We report the design and fabrication of a "signal-on" electrochemical aptamer-based (E-AB) sensor for detection of ampicillin. The signaling of the sensor is based on target binding-induced changes in the conformation and flexibility of the methylene blue-modified aptamer probe. The sensor's response is fast; signal saturation can be reached in ~ 200s. Since all the sensor components are surface-immobilized, it is regenerable and can be reused for at least three times. It has demonstrated good specificity and is capable of differentiating between ampicillin and structurally similar antibiotics such as amoxicillin. More importantly, it is selective enough to be employed directly in complex samples, including serum, saliva, and milk. Although both alternating current voltammetry (ACV) and square wave voltammetry (SWV) are suitable sensor characterization techniques, our results show that ACV is better suited for target analysis. Even under the optimal experimental conditions, the limit of detection of the sensor obtained in ACV (1µM) is significantly lower than that obtained in SWV (30µM).