Effect of antioxidants after post-space irrigation on the adhesive interface of glass fiber post cementation.

OBJECTIVE: This study evaluated the effects of antioxidants, 10% sodium ascorbate (SA) or 20% alpha-tocopherol (AT), after post-space irrigation with 2.5% sodium hypochlorite +17% EDTA (SH) or 1% peracetic acid (PA) on the adhesive interface after glass fiber post cementation. MATERIALS AND METHODS: Sixty bovine roots were endodontically treated. After preparation, the post-space was irrigated with SH or PA followed or not by the use of antioxidants (SA or AT) (n = 10). Push-out bond strength test, failure mode, and dentin penetrability analysis using confocal laser microscope were performed in the cervical, middle, and apical thirds. Data from bond strength and dentinal penetrability were evaluated by one-way ANOVA and Tukey post hoc test (p < 0.05). RESULTS: SH showed the lowest bond strength regardless of the third (p < 0.05). In apical third, mixed failure was the most incident in all groups. Only in the cervical third of the post-space, SH-AT provided the greatest tag extension of the cementation system into dentin (p < 0.05). However, in the middle and apical thirds, SH-AT, SH-SA, and PA-SA provided the largest tag extensions (p < 0.05), but similar to each other (p > 0.05). CONCLUSIONS: The use of antioxidants only favored bond strength when SH was used and dentin penetrability of the adhesive and conventional resin cementation, regardless of the solution used to irrigate the post-space. CLINICAL SIGNIFICANCE: The use of antioxidants (10% sodium ascorbate and 20% alpha-tocopherol) after post-space irrigation with sodium hypochlorite appears to increase the bond strength favoring the glass fiber post-cementation.

Impact of cleansing protocols to remove endodontic sealer residues on the adhesive interface: Bonding with universal adhesive systems.

OBJECTIVE: The aim of this study was to evaluate the effectiveness of cleansing protocols to remove sealer residues using different cleaning strategies and the effect of bond strength of two universal adhesives to dentin impregnated with epoxy resin-based sealer. MATERIALS AND METHODS: Fifty bovine dentin specimens were impregnated with epoxy resin-based sealer containing calcium hydroxide (Sealer Plus) and submitted to cleansing protocols (n = 10): negative control (NC), positive control (PC), 95% ethanol (ET), xylol (XI), and amyl acetate (AA). Specimens were evaluated by scanning electron microscope (SEM). Other 100 specimens were submitted to the same protocols (n = 20). Each protocol was divided into subgroups (n = 10) according to the universal adhesive system used: Scotchbond Universal (SU); Ambar Universal (AU). Bond strength was evaluated by micro-shear bond test (µSBT). Persistence of residues data were assessed with Kruskal-Wallis and Dunn's tests. µSBT data were analyzed with two-way ANOVA (α = 5%). RESULTS: ET presented higher persistence of residues compared to AA and XI (p < 0.05). AA and XI were similar from each other (p > 0.05). AU and SU presented similar µSBT values, regardless of the cleansing solution (p > 0.05). SU-NC presented the highest µSBT among all conditions (p < 0.05). CONCLUSION: The bond strength of universal adhesives was not affected by different amounts of sealer residues after different cleaning protocols. CLINICAL SIGNIFICANCE: Non-polar substances such as amyl acetate and xylol are effective for removing residues from epoxy resin-based endodontic sealers.

Cleaning and microstructural effects of amyl acetate on pulp chamber dentin impregnated with epoxy resin-based endodontic sealer.

OBJECTIVE: To evaluate the cleaning potential of 95% ethanol, acetone, and amyl acetate solutions used solely or in association, to remove epoxy resin-based sealer residues from pulp chamber dentin and their microstructural effects. MATERIALS AND METHODS: One hundred and eighty bovine incisor specimens were divided into nine groups according to the cleaning protocol: ET (ethanol); AC (acetone); AA (amyl acetate); E1: AA+AC; E2: AA+ET; E3: AC+ET; E4: AA+AC+ET; PC (positive control), and NC (negative control). All groups were impregnated with epoxy resin-sealer, except NC. Ninety specimens were divided into groups (n = 10) for evaluation of persistence of residues and amount of open dentinal tubules by SEM analysis and evaluation of chemical compounds on the dentin surface after cleaning with electron dispersive spectroscopy. The others 90 specimens were submitted to Knoop microhardness evaluation. Persistence of residues data were submitted to the Kruskal Wallis and Dunn tests (α = 0.05). Open dentinal tubules and microhardness data were submitted to one-way ANOVA and Mann Whitney tests (α = 0.05). RESULTS: AA and E4 protocols showed the lowest persistence of residues. E4 group had the highest incidence of open dentinal tubules. E3 and E4 groups showed no changes in the atomic ratio Ca/P, which was similar to NC group. E4 group did not present W, an element presents in all the other groups. ET and E4 protocols showed the smallest reduction in dentin microhardness. CONCLUSIONS: The combination of amyl acetate, acetone and ethanol is the most effective and safe protocol to remove epoxy sealer residues on pulp chamber dentin. Moreover, it has the lowest microhardness reduction. CLINICAL SIGNIFICANCE: The combined use of amyl acetate, acetone, and ethanol enhanced the cleaning of pulp chamber dentin with minimal microstructural damage.

Influence of different glaze firing protocols on the mechanical properties of CAD-CAM ceramic materials.

STATEMENT OF PROBLEM: The influence of different glaze firing protocols and cooling on the behavior of computer-aided design and computer-aided manufacturing (CAD-CAM) ceramics is unclear. PURPOSE: The purpose of this in vitro study was to investigate the influence of different glaze firing protocols on the abrasion resistance, surface roughness, microhardness, and brittleness index of CAD-CAM ceramics. MATERIAL AND METHODS: Rectangular specimens of IPS e.max CAD (MAX), IPS Empress CAD (PRE), and CEREC Blocs (CER) were obtained and divided into 5 groups according to the glaze firing protocol (n=8): control (CO), 1 conventional glaze firing (GF), 2 conventional glaze firings (GF2), 1 extended glaze firing (EG), and 2 extended glaze firings (EG2). The coefficient of friction (µ) was determined with the pin-on-disk test, and specimens were analyzed with an optical interferometer to calculate volume loss and roughness. Vickers microhardness and the brittleness index were obtained with a microdurometer. Microhardness data were evaluated by 2-way ANOVA and the Sidak tests (α=.05). Data from other tests were assessed with nonparametric ANOVA and Bonferroni tests (α=.05). RESULTS: Groups GF2, EG, and EG2 of MAX and PRE and all groups of CER presented higher levels of coefficient of friction. Groups EG and EG2 of MAX and CER showed high volume loss and surface roughness. Groups EG and EG2 of MAX and PRE showed lower hardness, as did all groups of CER. MAX-CO and groups GF and EG of PRE showed a lower brittleness index (P<.05), while CER groups showed no significant differences (P>.05). CONCLUSIONS: Ceramic specimens with conventional glaze firing presented less surface wear than those with extended glaze firing. The number of firings did not have a statistically significant influence.

Sealing of pulp chamber dentin in endodontics: Influence of bond strategy and time-point application.

Context: Seal the dentin of the pulp chamber during endodontic treatment to avoid interfering with the restorative treatment performed afterward. Aims: The aim was to evaluate the effect of three adhesive systems applied in different bonding strategies (etch-and-rinse, self-etch, and universal adhesive) and time-point application (immediately after the cavity access preparation or after endodontic obturation) on the hybrid layer formation and dentinal penetrability. Materials and Methods: Forty-eight sound molars were randomly distributed into six groups (n = 10) according to the adhesive system used: Forty-eight sound molars were randomly distributed into six groups (n = 10) according to the adhesive system used and the time-point application: Adper Scotchbond Multi-purpose (AS), Clearfil SE (CF) and Scotchbond Universal (SU) in strategy of immediate endodontic sealing (IES) or delayed endodontic sealing (DES). In IES-AS, IES-CF, and IES-SU groups, dentin sealing was performed immediately after the cavity access, while in DES-AS, DES-CF, and DES-SU, after root canal obturation. The specimens were sectioned in the long axis, in a buccal-lingual direction, and the dentinal penetrability of the adhesive systems was evaluated using confocal microscopy images. Hybrid layer formation was analyzed by scanning electron microscopy images. Statistical Analysis Used: Dentinal penetrability data were analyzed with the ANOVA test and the Kruskal-Wallis test was performed for hybrid layer data (α = 0.05). Results: IES-CF showed the lowest dentinal penetrability (P < 0.05), while the other protocols were similar to each other (P > 0.05). No significant differences were found between groups regarding the hybrid layer formation (P > 0.05). Immediate and DES protocols do not influence the hybrid layer formation, regardless of the bond strategy used. Conclusions: Sealing the pulp chamber dentin before endodontic treatment can improve the bond strength of the final restoration but the formation of the hybrid layer was not influenced by the bond strategy.

Fracture resistance and bonding performance after antioxidants pre-treatment in non-vital and bleached teeth.

This study aimed to evaluate the effect of antioxidant solutions on fracture strength and bonding performance in non-vital and bleached (38% hydrogen peroxide) teeth. One hundred and eighty dentin specimens were obtained, 60 for each test: fracture strength, hybrid layer thickness, and bond strength. The groups (n=10) were randomly composed according to post-bleaching protocol: REST - restoration, without bleaching; BL - bleaching + restoration; SA - bleaching, 10% sodium ascorbate solution, and restoration; AT - bleaching, 10% α-tocopherol solution, and restoration; CRAN - bleaching, 5% cranberry solution, and restoration; CAP - bleaching, 0.0025% capsaicin solution, and restoration. Data were analyzed with ANOVA, Kruskal-Wallis, Dunn, and Qui-Square tests (α=0.05). The highest fracture strength values were observed in REST (1508.96 ±148.15 N), without significant difference for the bleached groups (p>0.05), regardless of the antioxidant use. The hybrid layer thickness in the group that was not subjected to bleaching (REST) was significantly higher than in any other group. The bond strength in the bleached and antioxidants-treated groups (SA, AT, CRAN, CAP) has no differences with the bleached group without antioxidants (BL). Adhesive failures were predominant in the groups that did not receive the antioxidant application. In conclusion, the evaluated antioxidants did not show an effect on the fracture strength, hybrid layer thickness, or bond strength of dentin bleached after endodontic treatment. The application of 10% sodium ascorbate, 10% alpha-tocopherol, 5% cranberry, or 0.0025% capsaicin solutions is not an effective step and should not be considered for the restorative protocols after non-vital bleaching.

Microstructural effect of different concentrations of hydrogen peroxide photoactivated with LED/laser.

BACKGROUND: The use of different concentrations of hydrogen peroxide (HP) photoactivated with LED/laser sources is common however, their influence on tooth structure is not yet fully elucidated. This study aimed to evaluate the pH, microhardness and surface roughness of different bleaching protocols photoactivated with LED/laser. METHODS: Forty bovine incisors were sectioned (7 × 7 × 2 mm) and randomized into four groups for analysis of pH (n = 5), microhardness and roughness (n = 10): HP35, HP6_L, HP15_L, HP35_L. The pH analysis was performed in the initial and final minute of the bleaching protocol. Microhardness and roughness were evaluated before and 7 days after the last bleaching session. Results were obtained from two-way ANOVA for repeated measures and Bonferroni post-test at a significance level of 5%. RESULTS: HP6_L showed higher pH and greater stability between the initial and final evaluations, while the other groups showed similar pH with reduced values in the intragroup evaluation. No differences between groups in microhardness and roughness evaluations were observed. CONCLUSIONS: Although HP6_L showed higher alkalinity and pH stability, none of the protocols reduced the microhardness and surface roughness of bovine enamel.

Effect of sodium hypochlorite-based formulations on the adhesion interface after fiber post cementation.

The study evaluated the persistence of residues, bond strength and tags formation of a cementation system after post-space irrigation with different sodium hypochlorite-based irrigants. The groups were divided according to the irrigation protocol (DW: distilled water; SHS: sodium hypochlorite solution; SHG: sodium hypochlorite gel; and, SHT: sodium hypochlorite with surfactant). Forty roots (n=10) were used to evaluate the persistence of residues by scanning electron microscopy. Other forty roots were used to the push-out bond strength test, failure mode and tag formation analyses. Non-parametric data were submitted to Kruskal Wallis and Dunn tests, while parametric data were evaluated by one-way ANOVA. No difference was observed in the persistence of residues. DW showed the highest bond strength and tag formation. Type 2 failure mode was the most frequent in the experimental groups. Compared to control, SHT showed the best performance, since less negative effects on adhesive interface was observed.

Effect of irrigation protocols on chemical smear layer formation over the post-space dentin.

This study evaluated the effect of irrigation protocols using 95% ethanol (ET) or 1% peracetic acid (PA) prior the use of 2% chlorhexidine (CHX) compared to distilled water (DW) on the chemical smear layer (CSL) formation and incidence of open dentin tubules at the apical, medium, and cervical third of the post-space dentin. Scanning electron microscopy (SEM) and electron dispersive spectroscopy (EDS) images were used. Forty bovine roots were endodontically treated. After, post-space preparation was performed and the roots were randomized in four groups (n = 10) according to the irrigation protocol: DW, CHX, CHX-ET and CHX-PA. The chemical composition of CSL and the incidence of open dentin tubules at the post-space thirds were evaluated by EDS (500× magnification) and SEM (2000× magnification) images, respectively. Data from chemical composition of CSL were descriptively analyzed, while the incidence of open dentin tubules was evaluated by scores and submitted to Kruskal-Wallis and Dunn test (p = .05). Cl, Bi, and Si were the chemical elements most found over the dentin after the irrigation with CHX and CHX-ET. Moreover, CHX and CHX-ET showed the highest incidence of CSL (p < .05), but without difference between them (p > .05), regardless of the post-space third. DW and CHX-PA showed similar incidence of CSL (p > .05). No difference on the incidence of open dentin tubules was found for any irrigation protocol and post-space third (p > .05). The use of 1% PA prior the post-space irrigation with CHX decrease the incidence of CSL. RESEARCH HIGHLIGHTS: The post-space irrigation with chlorhexidine results in the formation of chemical smear layer. Ethanol is not capable to remove the chemical smear layer. Peracetic acid is more effective to remove the chemical smear layer.

Anti-erosive profile of an experimental 5% SnCl2 varnish containing different concentrations of NaF.

This in vitro study evaluated the anti-erosive effect of an experimental varnish containing 5% stannous chloride (SnCl2) associated with different concentrations of NaF (NaF-free, 2.5% NaF, or 5.2% NaF) on bovine enamel and root dentin. One hundred samples were pre-eroded (0.3% citric acid, pH 2.6, 10 min) and randomized into five groups (n=10 for each substrate): Negative control - milli-Q water; NaF-free - Experimental varnish SnCl2-free and NaF-free; 2.5 NaF - Experimental varnish 5% SnCl2 associated with 2.5% NaF; 5.2 NaF: Experimental varnish 5% SnCl2 associated with 5.2% NaF and positive control - Commercial varnish containing 5% NaF (Duraphat). After the varnishes were applied, the erosive and abrasive challenges were carried out for five days. Loss of tooth structure (TSL) was determined by optical profilometry, and the loss of calcium (ΔCa2+) using atomic absorption spectroscopy. Dentin analysis was also performed by SEM. A one-way ANOVA/Bonferroni test was performed to analyze the data (α=0.05). The experimental 2.5 NaF and 5.2 NaF groups showed greater effectiveness in preventing TSL when compared to the other groups (p <0.05), regardless of the substrate. In addition, these groups showed lower loss in Ca2+ content when compared to the other groups (p <0.05), for enamel and dentin. Dentin showed greater TSL and ΔCa2+ loss when compared to enamel in all treatments (p <0.05). The 5.2% and 2.5% NaF-containing experimental varnishes showed promising results in both, the prevention of TSL and the loss of Ca2+, regardless of the substrate studied.

Bonding effects of cleaning protocols and time-point of acid etching on dentin impregnated with endodontic sealer.

Objectives: This study aimed to investigate the bonding effects of cleaning protocols on dentin impregnated with endodontic sealer residues using ethanol (E) or xylol (X). The effects of dentin acid etching immediately (I) or 7 days (P) after cleaning were also evaluated. For bonding to dentin, universal adhesive (Scotchbond Universal; 3M ESPE) was used. The persistence of sealer residues, hybrid layer formation and microshear bond strength were the performed analysis. Materials and Methods: One hundred and twenty bovine dentin specimens were allocated into 4 groups (n = 10): G1 (E+I); G2 (X+I); G3 (E+P); and G4 (X+P). The persistence of sealer residues was evaluated by SEM. Confocal laser scanning microscopy images were taken to measure the formed hybrid layer using the Image J program. For microshear bond strength, 4 resin composite cylinders were placed over the dentin after the cleaning protocols. ANOVA followed by Tukey test and Kruskal-Wallis followed by Dunn test were used for parametric and non-parametric data, respectively (α = 5%). Results: G2 and G4 groups showed a lower persistence of residues (p < 0.05) and thicker hybrid layer than the other groups (p < 0.05). No bond strength differences among all groups were observed (p > 0.05). Conclusions: Dentin cleaning using xylol, regardless of the time-point of acid etching, provided lower persistence of residues over the surface and thicker hybrid layer. However, the bond strength of the universal adhesive system in etch-and-rinse strategy was not influenced by the cleaning protocols or time-point of acid etching.

Whitening efficacy of low concentration hydrogen peroxide photoactivated with blue or violet LED.

BACKGROUND: Bleaching protocols using low concentration hydrogen peroxide (HP) photoactivated with LED sources have been widely discussed. Herein, we evaluated the whitening efficacy of 15% HP photoactivated with blue or violet LED compared to 35% HP. METHODS: Thirty bovine crowns were sectioned into 5 × 5 × 2 mm specimens. After staining in black tea, the specimens were randomized into three groups (n = 10): 35% HP, 15% HP + blue LED and 15% HP + violet LED. Two bleaching sessions were performed and the color assessment (∆L*, ∆a*, ∆b*, ∆Eab [CIELab], ∆E00 [CIEDE2000] and WID) was performed before, 24h after each session, 7 days and 1 month after the last session. Data were evaluated by two-way repeated measures ANOVA and Bonferroni post-test at a significance level of 5%. RESULTS: All groups showed effective and similar results over 1 month of follow-up (p > 0.05), with only intragroup differences among the time intervals (p < 0.05). CONCLUSIONS: The use of 15% HP photoactivated with blue or violet LED showed similar whitening efficacy compared to 35% HP. Thus, the association of low concentration bleaching gels with blue or violet LED sources can provide successful and less-aggressive treatment in terms of color change.

Effect of mechanical cleaning protocols in the fiber post space on the adhesive interface between universal adhesive and root dentin.

The aim of this study was to evaluate the effects of cleaning protocols on the presence of residues, open dentinal tubules, and bond strength of a self-etch adhesive plus a dual-cure resin cement to dentin in the fiber-post space. The methods for cleaning utilized were conventional irrigation (CI), rotary brush with continuous rotary movement (RB), and continuous ultrasonic irrigation (CUI). Sixty bovine incisors were used for fiber-post space preparation. Thirty roots were divided into three groups (n = 10) according to the cleaning protocols and submitted to scanning electron microscope to evaluate presence of residues and relative quantity of open dentinal tubules on dentin surface. Similar protocols were performed in the other thirty roots (n = 10) using a self-etching adhesive system (Scotchbond Universal) and a dual-cure resin cement (Relyx Unicem 2). After 6 months cervical, middle, and apical thirds sections of fiber-post space were submitted to push-out testing. To data ANOVA and Tukey's test were employed (α = 5%). RB and CUI showed the lowest presence of residues on dentin surface (p = .015) and the highest bond strength values (p < .001), regardless of the dentin third. CUI presented the highest quantity of open dentinal tubules in middle and apical thirds (p < .001). CI presented the lowest bond strength value of resin cement, regardless of dentin third (p < .001). Mechanical cleaning protocols, such as RB and CUI, presented better surface cleaning capabilities and higher bond strength than CI with dual-cure resin cement employed. The use of a mechanical cleaning protocol seems to cause appropriate condition on the root dentin surface. Mechanical cleaning of root dentin provided greater bond strength of the self-etching system with dual resin cement. Conventional irrigation did not provide adequate root dentin cleaning. RESEARCH HIGHLIGHTS: • The use of a mechanical cleaning protocol seems to cause appropriate condition on the root dentin surface. • Mechanical cleaning of root dentin provided greater bond strength of the self-etching system with dual resin cement. • Conventional irrigation did not provide adequate root dentin cleaning.

Effects of tooth bleaching protocols with violet LED and hydrogen peroxide on enamel properties.

BACKGROUND: This study evaluated the color change, enamel surface roughness and microhardness after different tooth bleaching protocols, using hydrogen peroxide (HP) and/or violet LED. METHODS: Forty bovine specimens (7 × 7 × 2 mm) were randomly distributed into 4 groups: 35% HP, 6% HP, 6% HP + violet LED and violet LED alone. First, the specimens were stained with black tea and then submitted to two bleaching sessions of 30 min with an interval of 7 days. Color change (∆L*, ∆a*, ∆b* and ∆E00) after 24 h of each session and 1 week after the last session was evaluated. Enamel roughness and microhardness were evaluated immediately before the sessions, 24 h and 1 week after the last session. Data were evaluated by ANOVA for repeated measures and Bonferroni post-test or Kruskall-Wallis and Dunn tests (α = 0.05). Representative specimens from each group were analyzed by scanning electron microscopy. RESULTS: 6% HP + violet LED and 35% HP showed the highest color change, while violet LED alone had the lowest results. Enamel roughness analyses showed that 6% HP + violet LED and 35% HP showed changes after two bleaching sessions. No differences were observed regarding enamel microhardness. CONCLUSIONS: The use of 6% HP + violet LED showed enhanced bleaching efficacy compared to 35% HP. However, violet LED used alone exhibited the lowest color change. 6% HP + violet LED and 35% HP promoted changes on enamel roughness, while no microhardness changes was observed for any group.

Influence of agitation methods of irrigants after methylene blue-mediated PDT on the bonding interface of a fiber post cementation system.

BACKGROUND: This study evaluated the effects of final agitation methods of irrigants to remove methylene blue and sodium hypochlorite residues after PDT-assisted endodontic treatment on the bond strength of fiber posts cemented with etch-and-rinse adhesive and conventional resin cement. METHODS: Ninety bovine teeth were endodontically treated. In sequence, post space preparation followed by methylene blue-mediated PDT and sodium hypochlorite (NaOCl) irrigation were performed. Six final irrigations protocols for dye and NaOCl removal were performed prior to cementation with etch-and-rinse adhesive (Adper Scocthbond Multipurpose) and conventional dual resin cement (RelyX ARC): Conventional endodontic irrigation (CEI), passive ultrasonic irrigation (PUI), mechanical agitation with XP Endo Finisher (XPF), XP Clean (XPC) or Easy Clean (ECL) and distilled water (NCO - control). After fiber post cementation, push-out bond strength test was performed at different thirds of the post space. Failure mode was also analyzed. ANOVA and Tukey's post-hoc test was used for data analysis (α=5%). RESULTS: PUI, XPF e XPC protocols showed the highest bond strength values with no difference among them (p > 0.05), although they were similar to NCO, regardless of the post space third. CEI e ECL showed similar bond strength values, regardless of the third (p > 0.05). Adhesive failure was the most incident for CEI and ECL, while mixed and cohesive failures were predominant in PUI, XPF, XPC and NCO groups. CONCLUSIONS: Mechanical agitation of distilled water with XPF, XPC and PUI after methylene blue-mediated PDT and irrigation with 2.5% sodium hypochlorite promoted bond strength of the resin cementation system in post space dentin comparable to control group.

Impact of Finishing Protocols on Color Stability of CAD/CAM Ceramics: A Systematic Review.

PURPOSE: To analyze the current evidence on the impact of surface finishing protocols (such as manual polishing or glazing) on the color stability of in vitro simulated pigmentation of CAD/CAM ceramics. MATERIALS AND METHODS: Five electronic databases were searched on February 12, 2020. In vitro experimental studies were included based on the following strategy: intervention = surface treatment of CAD/CAM ceramics with glazing agents; comparison = surface treatment of CAD/CAM ceramics with manual polishing; and outcomes = color stability. Two reviewers independently assessed the risk of bias. RESULTS: Among 1,390 articles that were screened, 6 in vitro studies were considered for qualitative analysis. Five articles confirmed changes in the color of ceramics when they were immersed in pigmented solutions. One article investigated the color stability of the samples via ultraviolet (UV) aging. Among the 6 studies analyzed, 4 showed clinically acceptable color alteration values represented by ΔE irrespective of the finishing protocol applied (glazing or mechanical polishing). Two articles presented clinically unsatisfactory color variation (ΔE > 3.3 and ΔE > 2.7) following mechanical polishing of a zirconia-reinforced lithium silicate ceramic. Coffee and red wine proved to be the beverages with the greatest potential for ceramic pigmentation. Only 1 article had a high risk of bias. CONCLUSION: For clinicians, most of the studies demonstrated that both manual polishing and glaze application can prevent significant color alterations on CAD/CAM ceramic surfaces. However, due to the relatively limited amount of evidence to support this conclusion, further studies must be conducted.

LED/laser photoactivation enhances the whitening efficacy of low concentration hydrogen peroxide without microstructural enamel changes.

BACKGROUND: The association of low concentration hydrogen peroxide (HP) and a light source has been widely used to achieve efficient bleaching. We investigated the colorimetric and microstructural changes of bovine enamel bleached with 6% HP associated or not with a hybrid light source system of violet light and laser (LED/laser). METHODS: Twenty bovine crowns were used to obtain specimens of 7 × 7 × 2 mm. Then, they were randomized in two groups (n=10): 6% HP and 6% HP + LED/laser. After staining with dark tea solution, three bleaching sessions were performed. Colorimetric evaluation (∆L*, ∆a*, ∆b*, ∆E00 [CIEDE2000] and WID) after 24 hours of each session and 7 days after the final bleaching session was performed. Enamel Vickers microhardness at baseline, 24 hours and 7 days after the last bleaching session were also evaluated. Two-way repeated measures ANOVA and Bonferroni post-test was used at a significance level of 5%. RESULTS: 6% HP and 6% + HP LED/laser showed satisfactory bleaching results. The group photoactivated showed higher WID values (p<0.05). Differences between groups were observed for ∆E00, ∆L* and ∆a* (p<0.05), except for ∆b* (p>0.05). Intra-group differences were also found (p<0.05). Regarding microhardness, no inter or intra-group differences were observed (p>0.05). CONCLUSIONS: The photoactivation with LED/laser enhanced the whitening efficacy of 6% HP compared to the group without photoactivation. Thus, the LED/laser activation appears to be a good option when using low concentration HP-based agents. In addition, both bleaching protocols did not cause changes on enamel microhardness.

Hybrid layer formation and bond strength to dentin impregnated with endodontic sealer after cleaning protocols.

AIMS: This study evaluated the hybrid layer formation and bond strength of two adhesive systems, Scotchbond Universal (U) or Adper Scotchbond Multi Purpose (M), after cleaning protocols using ethanol (E) or xylol (X), to dentin impregnated with an epoxy-resin based endodontic sealer. SETTINGS AND DESIGN: The study design was an Experimental in vitro study. METHODOLOGY: One hundred bovine dentin specimens were randomly allocated into five groups (n = 10): Computed tomography (CT) (control): Only acid etching + M; E+U; X+U; E+M. After the specimen preparation, images were obtained using confocal laser scanning microscopy to evaluate the hybrid layer formation. For microshear bond strength test, the dentin specimens were included in polyvinyl chloride tubes and four resin composite cylinders were placed on the surface. The analysis was performed 24 h after storage. STATISTICAL ANALYSIS USED: For parametric and nonparametric data, analysis of variance followed by Tukey test and Kruskal-Wallis, followed by Dunn test were, respectively, used at a significance level of 5%. RESULTS: Regarding hybrid layer formation, all experimental groups were similar to each other (P > 0.05). However, CT showed higher hybrid layer formation than other groups (P < 0.05), except in relation to X+M (P > 0.05). Bond strength was statistically similar among all groups (P > 0.05). CONCLUSIONS: Hybrid layer formation in dentin impregnated with epoxy resin-based sealer and submitted to different cleaning protocols was similar to the control group only for X+M. No differences were found among the experimental groups. Regarding the bond strength, no effect was observed for any group.

Effect of post-space irrigation with acid solutions on bond strength and dentin penetrability using a self-adhesive cementation system.

BACKGROUND: The aim of this study was to evaluate the effects of surface treatments with 1% peracetic acid (PA), solution containing 17% EDTA (SmearClear, Kerr Endodontics), solution containing a combination of 17% EDTA with 2% chlorhexidine (QMix, Dentsply Sirona) on the post-space root dentin compared to 2.5% sodium hypochlorite (NaOCl) on bond strength and resin tags length in dentin. MATERIAL AND METHODS: Forty human-canine roots were endodontically treated and the post space was prepared. The specimens were randomised into four groups (n = 10): control - irrigation with 2.5% NaOCl solution, PA - irrigation with 1% PA, SmearClear - irrigation with SmearClear solution, and QMix - irrigation with QMix solution. The fibre posts were cemented using a self-adhesive resin system (Relyx U200, 3M ESPE). After six months, the specimens were cross-sectioned and subjected to push-out and confocal laser microscopy tests. One-way ANOVA and Tukey's tests were used to analyse the data (α= 0.05). RESULTS: PA and QMix presented the highest bond strength values compared to the other groups (p< 0.05). There was no significant difference between the resin tags length in dentin by the groups (p = 0.75). CONCLUSIONS: Irrigation of the post space with 1% PA and QMix showed a positive clinical impact on the adhesion between the fiber post and root dentin. However, these materials had no influence on resin tags length in dentin by self-adhesive resin cement. Key words:Fiber post, adhesive cementation, self-adhesive resin cement, root dentin, irrigating solutions.

Antimicrobial activity of probiotics against oral pathogens around orthodontic mini-implants: an in vitro study.

OBJECTIVE: The aim of this in vitro study was to evaluate the antimicrobial effect of five types of non-industrialized and industrialized probiotics on biofilms formed around orthodontic mini-implants. The null hypothesis tested was: there is no difference in the antimicrobial effect between the five types of probiotics tested around orthodontic mini-implants. METHODS: For the experiment, 120 mini-implants were immersed for seven days in Staphylococcus aureus solution for biofilm formation, and were subsequently plated in culture medium containing probiotics. The mini-implants were divided into six different groups, according to the probiotic used: G1)Lactobacillus casei; G2)Lactobacillus brevis; G3)Lactobacillus rhamnosus; G4) Lactobacillus from fermented milk Yakult®; G5) Lactobacillus from fermented milk Batavito® and G6) without use of probiotic, as negative control. Qualitative and quantitative analyses of all groups were performed using the CFU (colony forming unit) count. RESULTS: The study showed that groups G4 and G6 did not present antimicrobial activity, in comparison to groups G1, G2, G3, and G5 (p< 0.05), which demonstrated antimicrobial activity. CONCLUSION: The non-commercial probiotic bacteria, Lactobacillus casei and Lactobacillus rhamnosus, as well as commercially available fermented milk Batavito® presented promising results in the reduction of colonization of mini-implants by S. aureus. Therefore, the null hypothesis was rejected.