A long non-coding RNA TSLD8 inhibits hepatocellular carcinoma by stabilizing WWOX.

The hepatocellular carcinoma (HCC) is a common and highly aggressive malignancy especially in China. Accumulating data have shown a critical role of long non-coding RNAs (lncRNAs) during cancer progression. However, the function of lncRNA TSLD8 remains elusive. By lncRNA profiling, we identify a novel lncRNA termed TSLD8 in HCC. TSLD8 expression is significantly lowered in HCC tissues and cell lines. TSLD8 facilitates migration and viability in SMMC-7721 and HepG2 cells. Furthermore, TSLD8 can interact with WWOX and protect WWOX from proteasome-mediated degradation. Using PuPGEA-based nanocomplex for gene delivery, we found that co-delivery of TSLD8 and WWOX may exhibit synergistic and additive effects to inhibit HCC progression. PuPGEA-based nanocomplex delivery does not substantially alter the blood chemistries (e.g. alkaline phosphatase, blood urea nitrogen, aspartate aminotransferase, alanine aminotransferase) or initiate immune responses implying a safe strategy. Collectively, our current study has identified a novel tumor suppressive lncRNA TSLD8 which exerts its tumor suppressive function by stabilizing WWOX. Co-delivery of TSLD8 and WWOX via PuPGEA-based nanocomplexes might provide promising therapeutics for eradicating HCC.

Non-surgical treatment of maxillary lateral incisor double dens invaginatus type â ¢ with apical periodontitis.

Dental invagination is an abnormality of the crown or root development induced during tooth germ development when the enamel-forming apparatus or epithelial root sheath overpopulates and folds into the papilla. In severe cases, the invaginated channels are connected to the pulp and periodontal tissues, often causing endodontic and periapical diseases. The complex anatomical pattern of this disease adds difficulty in its preoperative diagnosis and clinical operation. In this paper, we report a case of non-surgical treatment assisted by cone beam CT and microscopy for maxillary lateral incisor double dens invaginatus type Ⅲ (Ⅲa and Ⅲb) with apical periapical infection. After 1-year follow-up, the affected tooth was asymptomatic and the periapical lesion was significantly reduced.

Meeting the Canadian 24-Hour Movement Guidelines and physical-mental comorbidity among Chinese children and adolescents: Prevalence, associations, and the population impacts.

OBJECTIVES: To examine the association between meeting the Canadian 24-Hour Movement Guidelines and physical-mental comorbidity among children and adolescents in a cross-sectional study. METHODS: A total of 21,061 students aged 11-17 years from Zhejiang Province, China was recruited in the study. We examined the coexistence of five specific physical illnesses - hypertension, high myopia, dental caries, scoliosis, and obesity - with mental illness, specifically depressive symptoms. Generalized linear mixed models were performed to assess the association between overall and specific combinations of movement guidelines and physical-mental comorbidity, presented by odds ratio (OR) and 95% confidence interval (CI). Population attributable fraction (PAF) was calculated to estimate the preventable proportion of comorbid cases via meeting all three movement recommendations. RESULTS: Of the included participants, 19.3% had physical-mental comorbidity. There were 3.8% and 17.0% meeting all three and none of the recommendations, respectively. Meeting at least one recommendation, except for moderate-to-vigorous physical activity recommendation only, was associated with a lower risk of physical-mental comorbidity, with ORs (95% CIs) ranging from 0.72 (0.66-0.79) to 0.40 (0.31-0.51). Meeting more recommendations was associated with decreased comorbid risks, and the association was stronger in 4th-6th graders. The association between specific combinations of recommendations and comorbid risks showed differences by gender and grade. Of the comorbid cases, 42.1% were attributed to not adhering to all three recommendations, and the PAFs varied from 27.4% to 55.7% across different genders and grades. CONCLUSION: Adherence to the 24-h movement guidelines was associated with lower risks of physical-mental comorbidity among children and adolescents.