Contamination characteristics of trace metals in dust from different levels of roads of a heavily air-polluted city in north China.

Concentrations of eight trace metals (TMs) in road dust (RD) (particles < 25 µm) from urban areas of Xinxiang, China, were determined by inductively coupled plasma mass spectrometry. The geometric mean concentrations of Zn, Mn, Pb, As, Cu, Cr, Ni and Cd were 489, 350, 114, 101, 60.0, 39.7, 31.6, and 5.1 mg kg-1, respectively. When compared with TM levels in background soil, the samples generally display elevated TM concentrations, except for Cr and Mn, and for Cd the enrichment value was 69.6. Spatial variations indicated TMs in RD from park path would have similar sources with main roads, collector streets and bypasses. Average daily exposure doses of the studied TMs were about three orders of magnitude higher for hand-to-mouth ingestion than dermal contact, and the exposure doses for children were 9.33 times higher than that for adults. The decreasing trend of calculated hazard indexes (HI) for the eight elements was As > Pb > Cr > Mn > Cd > Zn > Ni > Cu for both children and adults.

A Y-shape-structured electrochemiluminescence biosensor based on carbon quantum dots and locked nucleic acid probe for microRNA determination with single-base resolution.

Since microRNAs (miRNAs) are predictors of tumorigenesis, accurate identification and quantification of miRNAs with highly similar sequences are expected to reflect tumor diagnosis and treatment. In this study, a highly selective and sensitive electrochemiluminescence (ECL) biosensor was constructed for miRNAs determination based on Y-shaped junction structure equipped with locked nucleic acids (LNA), graphene oxide-based nanocomposite to enrich luminophores, and conductive matrix. Specifically, two LNA-modified probes were designed for specific miRNA recognition, that is, a dual-amine functionalized hairpin capture probe and a signal probe. A Y-shaped DNA junction structure was generated on the electrode surface upon miRNA hybridizing across the two branches, so as to enhance the selectivity. Carbon quantum dots-polyethylene imine-graphene oxide (CQDs-PEI-GO) nanocomposites were developed to enrich luminophores CQDs, and thus enhancing the ECL intensity. For indirect signal amplification, an electrochemically activated poly(2-aminoterephthalic acid) (ATA) film decorated with gold nanoparticles was prepared on electrode as an effective matrix to accelerate the electron transfer. The fabricated ECL biosensor achieved sensitive determination of miRNA-222 with a limit-of-detection (LOD) as low as 1.95 fM (S/N = 3). Notably, Y-shaped junction structures equipped with LNA probes endowed ECL biosensor with salient single-base discrimination ability and anti-interference capacity. Overall, the proposed Y-shaped ECL biosensor has considerable promise for clinical biomarker determination.

Release of microplastics from typical rainwater facilities during aging process.

As the demand for urban flood prevention and drainage increases, a large number of plastic rainwater facilities are in use. Microplastics will be released inevitably into stormwater systems during aging and hydraulic scouring processes, which could cause potential pollution risk. This study simulated the release behavior of microplastics from three typical plastic rainwater facilities including a rainwater inspection well, rainwater storage module, and rainwater pipe (mainly composed of high-density polyethylene, polypropylene, and polyvinyl chloride, respectively) under the effects of aging and hydraulic scouring. After 15-45 days of UV aging and 72 h of hydraulic scouring, the surfaces of the three facilities were found to exhibit increases in roughness, cracks, folds, and cavities, with the most pronounced changes occurring in the rainwater storage module. As the aging time increased, oxygen-containing functional groups formed and led to carbon chain scission. Fourier transform infrared spectroscopy (FTIR), two-dimensional correlation spectroscopy (2D-COS) and X-ray photoelectron spectroscopy (XPS) of facility surfaces showed that the formation of oxygen-containing functional groups was an important factor affecting the release of microplastics. The amount of microplastics released from the three facilities ranged from 160 to 1905 items/g (microplastics/facilities), following in the order of rainwater inspection well > rainwater storage module > rainwater pipe. The particle size of the released microplastics ranged from 3 to 1363 µm, with 10-30 µm accounting for the greatest proportion of particles, 50.10%. The size of microplastics released from the rainwater inspection well and rainwater storage module increased with the aging degree, while the release from the rainwater pipe decreased. The release behavior depends mainly on the composition of the materials and the aging time. Thus, microplastics can be released from plastic rainwater facilities under suitable conditions. The results can be used to further evaluate microplastic pollution caused by urban rainwater facilities.

Assessment of Occupational Exposure in Medical Practice in the Region of Hohhot, China, for the Period 2004-2020.

ABSTRACT: Individual monitoring is of great significance in efforts to protect the health of radiation workers and improve the level of radiation protection and management. This paper presents a retrospective analysis of occupational exposure to ionizing radiation from medical practice in the region of Hohhot, China, from 2004 to 2020. Results show that the average annual effective dose of occupationally exposed workers in medical practice significantly declined from 1.44 mSv in 2005 to 0.29 mSv in 2020 (Z = -5.23, P < 0.05). The number of medical radiation workers increased by 181%, the composition of radiation workers whose average annual effective dose exceeded 1 mSv decreased, and the number of radiation workers whose average annual effective dose was less than or equal to the minimum detection level (MDL) increased yearly over the 17-y study period. It was found that the dose of 1.106 mSv received by workers in interventional radiology is significantly higher than the doses of 0.52 mSv in dental radiology, 0.47 mSv in radiotherapy, and 0.33 mSv in all other medical uses (Z = 3.71, 9.13, 5.93, respectively; P < 0.05). The distribution ratios of workers in nuclear medicine and interventional radiology whose annual individual effective dose exceeded 5 mSv were 0.040 and 0.043, respectively, which are significantly higher than those in other occupational categories (χ2 = 307.11, P < 0.05). It was also shown that the average annual effective dose of 0.67 mSv in interventional radiology is significantly higher than that of 0.17 mSv in radiotherapy (Z = 3.39, P < 0.05) in 2020. According to these observations, the exposure of radiation workers in medical practice in Hohhot meets the requirements of the China standard. This study shows that the status of radiation workers in medical practice has obviously improved during the period 2004-2020. However, it is still necessary to focus on the protection of groups with high occupational exposure risk, and the continuous improvement of protection measures, monitoring means, and radiation workers' training, especially for the workers in the fields of interventional radiology and nuclear medicine.

Polarization insensitive 25-Gbaud direct D(Q)PSK receiver based on polymer planar lightwave hybrid integration platform.

We report a direct DPSK receiver based on polymer planar lightwave circuit technology, which incorporates a 2x25 GHz photodiode (PD) array hybridly integrated via 45° mirrors. In this direct DPSK receiver, a half-wave plate and heating electrodes are implemented to eliminate the polarization-dependent frequency-shift (PDFS) of the delay-line interferometer (DLI). By applying a proper heating current, a residual PDFS of practically zero at 1550 nm and within ±125 MHz was achieved over the full C-band. Integrated with the PD array, the peak responsivity is ~0.14 A/W for orthogonal polarizations over the C-band. To characterize this direct receiver, we introduce an adapted common-mode rejection ratio (CMRR), which takes into account the unequal responsivities of the PDs, the uneven split of the input power by the DLI, the phase error and the extinction ratio of the DLI. The measured CMRR under DC condition is below -20 dB over the C-band.

Hierarchical Assembly of Two-Dimensional Polymers into Colloidosomes and Microcapsules.

Hierarchical assembly of two-dimensional (2D) polymers to 3D microstructures provides new means of creating functional materials with exotic properties for extensive applications. Herein, we report an approach of assembling 2D covalent organic framework (COF) colloidosomes or microcapsules from small molecules. We polymerized monomers to produce narrowly distributed COF particles with average particle sizes greater than 490 nm, which were further used as stabilizers to prepare various water-in-oil Pickering emulsions with droplet sizes of 10-120 µm on average. The emulsion droplets were subsequently applied as templates for interfacial polymerization of the same monomers. The COF microcapsules with varied diameters and shell thicknesses of 0.2-3.1 µm were thus obtained, which possessed good stability, high crystallinity, and surface areas no less than 540 m2/g. The approach also permits facile loading of water-soluble substances such as salts, dyes, or proteins. The loaded molecules demonstrated different permeability against the shell, in which 98% of the encapsulated salts could be released in 1 h while only 18% of dye molecules and almost none of the fluorescent proteins diffused out from the microcapsules. Such an assembling approach may greatly extend the applications of 2D polymers and their microcapsules.

Effects of statins on cytokines levels in gingival crevicular fluid and saliva and on clinical periodontal parameters of middle-aged and elderly patients with type 2 diabetes mellitus.

OBJECTIVE: To analyze the effect of statins on cytokines levels in gingival crevicular fluid (GCF) and saliva and on clinical periodontal parameters of middle-aged and elderly patients with type 2 diabetes mellitus (T2DM). METHODS: Systemically healthy controls (C group, n = 62), T2DM patients not taking statins (D group, n = 57) and T2DM patients taking statins (S group, n = 24) were recruited. In each group, subjects (40-85 years) were subclassified into the h (periodontal health)group, the g (gingivitis)group or the p (periodontitis) group according to different periodontal conditions. 17 cytokines in gingival crevicular fluid (GCF) and saliva samples of each subject were measured utilizing the Luminex technology kit. Further, HbA1c (glycated hemoglobin), FPG (fasting plasma glucose), PD (probing depth), CAL (clinical attachment level), BOP (bleeding on probing), GI (gingival index) and PI (periodontal index) were recorded. Data distribution was tested through the Shapiro-Wilk test, upon which the Kruskal-Wallis test was applied followed by Mann-Whitney U test and Bonferroni's correction. RESULTS: Levels of IFN-γ, IL-5, IL-10 and IL-13 in the saliva of the Dh group were significantly lower than those in the Ch group, while factor IL-4 was higher (p<0.05). Levels of MIP-3α, IL-7 and IL-2 in GCF of the Dh group were considerably higher than those in the Ch group (p<0.05), while that of IL-23 was considerably lower. Compared with the Cg group, levels of IFN-γ, IL-4, IL-5, IL-6, IL-10 and IL-13 were significantly lower in the saliva of the Dg group (p<0.05). Lower levels of IFN-γ, IL-5 and IL-10 were detected in the Sg group than those in the Cg group (p<0.05). At the same time, levels of IL-1ß, IL-6, IL-7, IL-13, IL-17, IL-21 and MIP-3α in the gingival crevicular fluid of the Sg group were lower in comparison with the Dg group. In addition, lower levels of IL-4 and higher levels of IL-7 in GCF were identified in the Dg group than those in the Cg group, while in the Sg group, lower levels of IL-4, MIP-1αand MIP-3αwere observed than those in the Cg group (p<0.05). Lower levels of IFN-γ, IL-6, IL-10, IL-13 and I-TAC were found in the Sp group compared with those in the Cp group. The IFN-γ, IL-6 and IL-10 levels were lower in the Dp group than those in the Cp group (p<0.05). Meanwhile, in the Sp group, lower levels of pro-inflammatory factors IFN-γ, IL-1ß, IL-2, IL-6, IL-7, IL-21 and TNF-α, in addition to higher levels of anti-inflammatory factors IL-4 and IL-5 in gingival crevicular fluid, were identified than those in the Dp group. Higher levels of IFN-γ,IL-1ß,IL-2,IL-7,IL-21 and TNF-α and a lower level of IL-5 in the Dp group were identified than those in the Cp group (p<0.05). Moreover, statins were able to substantially reduce PD in T2DM patients with periodontitis, indicating an obvious influence on the levels of cytokines secreted by Th1 cells, Th2 cells and Th17 cells, as revealed by PCA (principal component analysis). CONCLUSION: Statins are associated with reduced PD and cytokines levels in the GCF and saliva of T2DM patients with periodontitis.

Silver Nanowire-Bacterial Cellulose Composite Fiber-Based Sensor for Highly Sensitive Detection of Pressure and Proximity.

Fiber-based sensors are desirable to provide an immersive experience for users in the human-computer interface. We report a hierarchically porous silver nanowire-bacterial cellulose fiber that can be utilized for sensitive detection of both pressure and proximity of human fingers. The conductive fiber was synthesized via continuous wet-spinning at a speed of 20 m/min, with a diameter of 53 µm, the electrical conductivity of 1.3 × 104 S/cm, a tensile strength of 198 MPa, and elongation strain of 3.0% at break. The fibers were coaxially coated with a 10 µm thick poly(dimethylsiloxane) dielectric elastomer to form the fiber sensor element which is thinner than a human hair. Two of the sensor fibers were laid diagonally, and the capacitance changes between the conductive cores were measured in response to pressure and proximity. In the touch mode, a fiber-based sensor experienced monotonic capacitance increase in the pressure range from 0 to 460 kPa, and a linear response with a high sensitivity of 5.49 kPa-1 was obtained in the low-pressure regime (<0.5 kPa). In touchless mode, the sensor is highly sensitive to objects at a distance of up to 30 cm. Also, the fiber can be easily stitched into garments as comfortable and fashionable sensors to detect heartbeat and vocal pulses. A fiber sensor array is able to serve as a touchless piano to play music and accurately determine the proximity of an object. A 2 × 2 array was further shown for two- and three-dimensional location detection of remote objects.

Biodegradable poly (lactic acid-co-glycolic acid) scaffolds as carriers for genetically-modified fibroblasts.

Recent advances in gene delivery into cells allow improved therapeutic effects in gene therapy trials. To increase the bioavailability of applied cells, it is of great interest that transfected cells remain at the application site and systemic spread is minimized. In this study, we tested clinically used biodegradable poly(lactic acid-co-glycolic acid) (PLGA) scaffolds (Vicryl & Ethisorb) as transient carriers for genetically modified cells. To this aim, we used human fibroblasts and examined attachment and proliferation of untransfected cells on the scaffolds in vitro, as well as the mechanical properties of the scaffolds at four time points (1, 3, 6 and 9 days) of cultivation. Furthermore, the adherence of cells transfected with green fluorescent protein (GFP) and vascular endothelial growth factor (VEGF165) and also VEGF165 protein secretion were investigated. Our results show that human fibroblasts adhere on both types of PLGA scaffolds. However, proliferation and transgene expression capacity were higher on Ethisorb scaffolds most probably due to a different architecture of the scaffold. Additionally, cultivation of the cells on the scaffolds did not alter their biomechanical properties. The results of this investigation could be potentially exploited in therapeutic regiments with areal delivery of transiently transfected cells and may open the way for a variety of applications of cell-based gene therapy, tissue engineering and regenerative medicine.

Bioresorption and degradation of biomaterials.

The human body is a composite structure, completely constructed of biodegradable materials. This allows the cells of the body to remove and replace old or defective tissue with new material. Consequently, artificial resorbable biomaterials have been developed for application in regenerative medicine. We discuss here advantages and disadvantages of these bioresorbable materials for medical applications and give an overview of typically used metals, ceramics and polymers. Methods for the quantification of bioresorption in vitro and in vivo are described. The next challenge will be to better understand the interface between cell and material and to use this knowledge for the design of "intelligent" materials that can instruct the cells to build specific tissue geometries and degrade in the process.

Cell-based resorption assays for bone graft substitutes.

The clinical utilization of resorbable bone substitutes has been growing rapidly during the last decade, creating a rising demand for new resorbable biomaterials. An ideal resorbable bone substitute should not only function as a load-bearing material but also integrate into the local bone remodeling process. This means that these bone substitutes need to undergo controlled resorption and then be replaced by newly formed bone structures. Thus the assessment of resorbability is an important first step in predicting the in vivo clinical function of bone substitute biomaterials. Compared with in vivo assays, cell-based assays are relatively easy, reproducible, inexpensive and do not involve the suffering of animals. Moreover, the discovery of RANKL and M-CSF for osteoclastic differentiation has made the differentiation and cultivation of human osteoclasts possible and, as a result, human cell-based bone substitute resorption assays have been developed. In addition, the evolution of microscopy technology allows advanced analyses of the resorption pits on biomaterials. The aim of the current review is to give a concise update on in vitro cell-based resorption assays for analyzing bone substitute resorption. For this purpose models using different cells from different species are compared. Several popular two-dimensional and three-dimensional optical methods used for resorption assays are described. The limitations and advantages of the current ISO degradation assay in comparison with cell-based assays are discussed.

Bioactivation of dermal scaffolds with a non-viral copolymer-protected gene vector.

The use of scaffolds in skin tissue engineering is accompanied with low regeneration rates and high risk of infection. In this study, we activated an FDA-approved collagen scaffold for dermal regeneration by incorporation of copolymer-protected gene vectors (COPROGs) to induce a temporary release of VEGF. In vitro results show that the presence of COPROGs did not affect the distribution, attachment, proliferation and viability of cells in the scaffold. A transient release of VEGF was observed for up to 3 weeks. Moreover a high amount of VEGF was also found in the cells and associated with the scaffold. In a full skin defect model in nude mice, VEGF levels were significantly increased compared to controls in VEGF gene activated scaffolds 14 d after implantation, but not in skin from the wound edge. Results showed an increased amount of non-adherent cells, especially erythrocytes, and von Willebrandt factor (vWF) and a yellow red appearance of gene activated scaffolds in relation to controls. This suggests the presence of leaky vessels. In this work we show that the bioactivation of collagen scaffolds with COPROGs presents a new technology that allows a local release of therapeutic proteins thus enhancing the regenerative potential in vivo.