RESUMO
Enterovirus A71 (EV-A71), one of the major pathogens that causes hand, foot and mouth disease (HFMD), has seriously threatened the health and safety of young children. In this study, aminothiazole derivatives were synthesized and screened against EV-A71 in Rhabdomyosarcoma (RD) cells. The best compound (12s), with a biphenyl group, showed activity against EV-A71 (EC50: 0.27 µM) but also against a series of different human enteroviruses without significant cytotoxicity (CC50 > 56.2 µM). Mechanistic studies including time-of-drug-addition assays, viral entry assays and microscale thermophoresis (MST) experiments, showed that 12s binds to EV-A71 capsid and blocks the binding between the viral protein VP1 and the relevant human scavenger receptor class B member 2 (hSCARB2).
Assuntos
Proteínas do Capsídeo , Enterovirus Humano A , Tiazóis , Proteínas do Capsídeo/antagonistas & inibidores , Enterovirus Humano A/efeitos dos fármacos , Infecções por Enterovirus/tratamento farmacológico , Humanos , Tiazóis/farmacologia , Internalização do VírusRESUMO
OBJECTIVE: To study the osteogenesis and ototoxicity of autogenous bone cement. STUDY DESIGN: A randomized, prospective, controlled animal study. SETTING: University laboratory. SUBJECTS AND METHODS: We created a critical defect on the bulla wall of guinea pigs. Fifty-three guinea pigs were divided into 3 groups. The defects were filled with autogenous bone cement (ABC) or autogenous bone dust (ABD) on the left side while the contralateral sides were kept intact as the blank control (BC) group or a defect was induced without obliteration as the operation control (OC) group (groups 1 and 2, n = 25 each). The animals were sacrificed at 2, 4, 8, and 12 weeks postoperatively. Group 3 (n = 3) was the ototoxicity positive control (OPC) group. The critical defects were evaluated for bone repair by histologic observation and computed tomography (CT), and the ototoxicity of the material was assessed by measuring shifts in the hearing threshold and changes in cochlear morphology. Antibiotic susceptibility testing of the material was also performed. RESULTS: Histologic observation and CT scans revealed complete bone repair in the ABC group at 12 weeks postoperatively, but progress was slower in the ABD group. The hearing threshold and cochlear morphology did not differ significantly between the preoperative and 12-week postoperative measurement. Autogenous bone dust inhibited some of the bacterial species commonly associated with chronic suppurative otitis media. CONCLUSIONS: Animals treated with ABC exhibited osteogenesis, with no ototoxicity and bacteriostasis compared with the use of ABD.