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1.
Rheumatology (Oxford) ; 59(10): 3070-3080, 2020 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-32417913

RESUMO

OBJECTIVES: IL-37 has been identified as an important anti-inflammatory and immunosuppressive factor. This study was undertaken to explore how IL-37 affects M1/M2-like macrophage polarization and thus contributes to anti-inflammatory processes in the temporomandibular joint. METHODS: Western blotting, quantitative real-time PCR (qRT-PCR) and immunofluorescence were used to verify the IL-37-induced polarization shift from the M1 phenotype to the M2 phenotype, and the related key pathways were analysed by western blotting. Human chondrocytes were stimulated with M1-conditioned medium (CM) or IL-37-pretreated M1-CM, and inflammatory cytokines were detected. siRNA-IL-1R8 and MCC-950 were used to investigate the mechanism underlying the anti-inflammatory effects of IL-37. Complete Freund's adjuvant-induced and disc perforation-induced inflammation models were used for in vivo studies. Haematoxylin and eosin, immunohistochemical and safranin-O staining protocols were used to analyse histological changes in the synovium and condyle. RESULTS: Western blotting, qRT-PCR and immunofluorescence showed that IL-37 inhibited M1 marker expression and upregulated M2 marker expression. Western blotting and qRT-PCR showed that pretreatment with IL-37 suppressed inflammatory cytokine expression in chondrocytes. IL-37 inhibited the expression of NLRP3 and upregulated the expression of IL-1R8. Si-IL-1R8 and MCC-950 further confirmed that the anti-inflammatory properties of IL-37 were dependent on the presence of IL-1R8 and NLRP3. In vivo, IL-37 reduced synovial M1 marker expression and cartilage degeneration and increased M2 marker expression. CONCLUSION: IL-37 shifting of the polarization of macrophages from the pro-inflammatory M1 phenotype to the beneficial anti-inflammatory M2 phenotype seems to be a promising therapeutic strategy for treating temporomandibular joint inflammation.


Assuntos
Polaridade Celular/efeitos dos fármacos , Interleucina-1/farmacologia , Ativação de Macrófagos/efeitos dos fármacos , Macrófagos/efeitos dos fármacos , Proteína 3 que Contém Domínio de Pirina da Família NLR/metabolismo , Transtornos da Articulação Temporomandibular/terapia , Western Blotting , Condrócitos/efeitos dos fármacos , Condrócitos/metabolismo , Meios de Cultivo Condicionados/farmacologia , Citocinas/metabolismo , Adjuvante de Freund , Furanos , Compostos Heterocíclicos de 4 ou mais Anéis/farmacologia , Humanos , Indenos , Inflamassomos/efeitos dos fármacos , Macrófagos/metabolismo , Côndilo Mandibular/patologia , Osteoartrite/induzido quimicamente , Osteoartrite/metabolismo , Osteoartrite/patologia , Osteoartrite/terapia , RNA Interferente Pequeno/farmacologia , Reação em Cadeia da Polimerase em Tempo Real , Receptores de Interleucina-1/metabolismo , Sulfonamidas , Sulfonas/farmacologia , Membrana Sinovial/patologia , Transtornos da Articulação Temporomandibular/induzido quimicamente , Transtornos da Articulação Temporomandibular/metabolismo , Transtornos da Articulação Temporomandibular/patologia , Regulação para Cima
2.
Artigo em Inglês | MEDLINE | ID: mdl-32693685

RESUMO

The toxic effects of various substances on Daphnia magna (D. magna) observed through traditional waterborne uptake may involve alterations to the nutritional quality of the contaminated algae and culture media. It is essential to find an alternative delivery method that will not affect the nutritional quality of D. magna's diet in order to elucidate the mechanisms of dietary metal toxicity. Therefore, this study examined the application of liposome encapsulation on the dietary toxicity of D. magna. Ag+-laden liposomes were prepared and the Ag encapsulation efficiency and inhibition effect on algae growth were examined. Then, acute and 14-day subchronic studies were performed to examine the effect of Ag+-laden liposomes on D. magna. The EC50 for the 24 h immobilization test was 10.59 µg/L for Ag+-laden liposomes and 3.07 µg/L for Ag+. In terms of subchronic effects, the estimated ECx values under the Ag+-laden liposome condition were always higher than the direct exposure condition. Furthermore, the bioaccumulation of Ag+-laden liposomes was about 1.68 times lower than direct exposure. Generally, Ag+-laden liposomes produced less efficient toxicity than direct exposure, e.g., lower D. magna mortality, production of more neonates, higher intrinsic rate of natural increase (rm), earlier time to first brood, and higher enzyme activities.


Assuntos
Daphnia/efeitos dos fármacos , Prata/toxicidade , Animais , Cápsulas , Lipossomos/química , Prata/química
3.
BMC Genomics ; 20(1): 600, 2019 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-31331262

RESUMO

BACKGROUND: Gerbera hybrida is one of the most popular cut flowers in the world; however, stem bending, which always happens when gerbera flower harvested from the field, greatly limits its vase life. To date the molecular mechanisms underlying stem bending remain poorly understood. RESULTS: In this study, we performed high-throughput transcriptome sequencing of gerbera during stem bending using the Illumina sequencing technology. Three cDNA libraries constructed from mRNAs of gerbera stem at stem bending stage 0, 2 and 4 were sequenced. More than 300 million high-quality reads were generated and assembled into 96,492 unigenes. Among them, 34,166 unigenes were functionally annotated based on similarity search with known protein. Sequences derived from plants at different stem bending stages were mapped to the assembled transcriptome, and 9,406 differentially expressed genes (DEGs) were identified. Through Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, specific pathways were identified during the stem bending process, such as phenylpropanoid biosynthesis pathway, phenylalanine metabolism pathway, starch and sucrose metabolism pathway, and plant hormone signal transduction pathway. A total of 211 transcription factors (TFs), including TF families involved in plant senescence, such as NAC, MYB, WRKY, and AP2/ERF members, as well as TFs related to water stress tolerance, were shown to be regulated during stem bending. Gene Onotology (GO) functional enrichment analysis indicated that key genes involved in responses to osmotic and oxidative stresses were also varied in expression during this process. Furthermore, analysis of DEGs involved in the hormone signaling pathways and determination of endogenous abscisic acid (ABA) content showed that stem bending may be an ethylene-independent process, but regulated by ABA. In short, our findings suggested that the stem bending of cut gerbera may be caused by the involvement of water stress and regulation of ABA during the postharvest life. CONCLUSIONS: The transcriptome sequences provide a valuable resource in revealing the molecular mechanism underlying stem bending of cut flower and offer novel genes that can be used to guide future studies for ornamental plant breeding.


Assuntos
Ácido Abscísico/metabolismo , Asteraceae/anatomia & histologia , Asteraceae/genética , Desidratação/genética , Perfilação da Expressão Gênica , Caules de Planta/anatomia & histologia , Asteraceae/metabolismo , Asteraceae/fisiologia , Lignina/biossíntese , Pressão Osmótica , Estresse Oxidativo/genética , Reguladores de Crescimento de Plantas/metabolismo , Análise de Sequência , Transdução de Sinais/genética , Fatores de Transcrição/metabolismo
4.
J Oral Maxillofac Surg ; 77(8): 1582-1593, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30904552

RESUMO

PURPOSE: Idiopathic condylar resorption (ICR) is an aggressive form of osteoarthritis that is frequently observed in adolescent female patients. We hypothesized that an estrogen-mediated pathway may contribute to ICR development. MATERIALS AND METHODS: An enzyme-linked immunosorbent assay was used to detect the levels of estradiol (E2) and hyaluronan in synovial fluid. Immunohistochemistry, real-time polymerase chain reaction, and Western blotting were used to detect the expression of microRNAs (miRNAs) and related genes after transfection of miRNA-101-3p mimics, inhibitor, or short interfering RNA into synovial fibroblasts. Dual-luciferase activity was determined to identify the direct effect of miRNA-101-3p on hyaluronan synthase 2 (HAS2). Linear regression analysis, the nonparametric Mann-Whitney U test, the Student t test, and 1-way analysis of variance were carried out to analyze the results of each group. RESULTS: The relationship between hyaluronan and E2 was negatively correlated in synovial fluid (Pearson r = -0.3179, P = .0230). Among the screened miRNAs, miRNA-101-3p was the most overexpressed in ICR. E2 mostly upregulated the expression of miRNA-101-3p at a dose of 10 nmol/L 12 hours after transfection in synovial fibroblasts of patients with ICR. However, E2 induction of miRNA-101-3p expression was significantly repressed by estrogen receptor α interference (P = 0.0286). The dual-luciferase assay showed that miRNA-101-3p regulated the expression of HAS2 by directly targeting its 3' untranslated region. CONCLUSIONS: We speculate that E2 regulates HAS2 expression by targeting miRNA-101-3p in synovial fibroblasts of patients with ICR. Thus, the E2-miRNA-101-3p-HAS2 pathway might play an important role in the pathogenesis of ICR.


Assuntos
Reabsorção Óssea , Estrogênios , Hialuronan Sintases , MicroRNAs , Osteoartrite , Adolescente , Reabsorção Óssea/genética , Reabsorção Óssea/patologia , Estrogênios/fisiologia , Feminino , Fibroblastos/metabolismo , Humanos , Hialuronan Sintases/metabolismo , Côndilo Mandibular , MicroRNAs/metabolismo
5.
Biomater Transl ; 4(3): 142-150, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-38283090

RESUMO

Periodontitis is a prevalent oral disease. It can cause tooth loss and has a significant impact on patients' quality of life. While existing treatments can only slow the progression of periodontitis, they are unable to achieve complete regeneration and functional reconstruction of periodontal tissues. As a result, regenerative therapies based on biomaterials have become a focal point of research in the field of periodontology. Despite numerous studies reporting the superiority of new materials in periodontal regeneration, limited progress has been made in translating these findings into clinical practice. This may be due to the lack of appropriate animal models to simulate the tissue defects caused by human periodontitis. This review aims to provide an overview of established animal models for periodontal regeneration, examine their advantages and limitations, and outline the steps for model construction. The objective is to determine the most relevant animal models for periodontal regeneration based on the hypothesis and expected outcomes.

6.
J Stomatol Oral Maxillofac Surg ; 125(4): 101751, 2023 Dec 24.
Artigo em Inglês | MEDLINE | ID: mdl-38145836

RESUMO

OBJECTIVE: To compare the dentofacial characteristics of children with and without adenoid and/or tonsillar hypertrophy. METHODS: A consecutive sample of orthodontic patients aged 6-12 that took pre-treatment lateral cephalograms were included in this study. Those with history of previous orthodontic treatment, adenoidectomy or tonsillectomy, or craniofacial anomalies were excluded. The diagnosis of adenoid and tonsillar hypertrophy was based on Fujioka's and Baroni's methods, according to which the subjects were divided into four groups: the adenoid hypertrophy only (AHO) group; tonsillar hypertrophy only (THO) group; combined adenoid and tonsillar hypertrophy (AH+TH) group; and no adenoid or tonsillar hypertrophy (NH) group. Cephalograms were used for skeletal and dental measurement. Data were analyzed using one-way ANOVA, LSD post-hoc tests and Chi-square test. RESULTS: A total of 598 patients were included. Compared with the NH group, the THO group had significantly larger SNB angle (P < 0.001), as well as significantly smaller ANB angle (P<0.001) and Wits value (P = 0.001). The U1-L1 angle of AHO group was significantly smaller than that in the NH group (P = 0.035). The proportion of adenoid hypertrophy in Class II patients was significantly higher than that in Class III patients (P = 0.001). The proportion of tonsillar hypertrophy in Class III patients was significantly higher than that in Class I patients (P < 0.001) and Class II patients (P < 0.001). CONCLUSION: Over 80 % of children seeking orthodontic treatment had either adenoid or tonsillar hypertrophy. Children with adenoid hypertrophy tend to have skeletal Class II malocclusion, while those with tonsillar hypertrophy tend to have skeletal Class III malocclusion.

7.
Chem Asian J ; 17(19): e202200677, 2022 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-35950549

RESUMO

Materials with negative Poisson's ratio have attracted considerable attention and offered high potential applications as biomedical devices due to their ability to expand in every direction when stretched. Although negative Poisson's ratio has been obtained in various base materials such as metals and polymers, there are very limited works on hydrogels due to their intrinsic brittleness. Herein, we report the use of methacrylated cellulose nanocrystals (CNCMAs) as a macro-cross-linking agent in poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogels for 3D printing of auxetic structures. Our developed CNCMA-pHEMA hydrogels exhibit significant improvements in mechanical properties, which is attributed to the coexistence of multiple chemical and physical interactions between the pHEMA and CNCMAs. Structures printed by using CNCMA-pHEMA hydrogels show auxetic behavior with greatly enhanced toughness and stretchability compared to the hydrogel with a traditional cross-linking agent. Such strong and tough auxetic hydrogels would contribute toward establishing advanced flexible implantable devices such as biodegradable oesophageal self-expandable stents.


Assuntos
Hidrogéis , Poli-Hidroxietil Metacrilato , Celulose , Hidrogéis/química , Poli-Hidroxietil Metacrilato/química , Impressão Tridimensional
8.
Bone ; 164: 116522, 2022 11.
Artigo em Inglês | MEDLINE | ID: mdl-35981698

RESUMO

As the main cells in endochondral osteogenesis, chondrocytes have limited self-repair ability due to weak proliferation activity, low density, and dedifferentiation tendency. Here, a thorough inquiry about the effect and underlying mechanisms of methyltransferase like-3 (Mettl3) on chondrocytes was made. Functionally, it was indicated that Mettl3 promoted the proliferation and hypertrophic differentiation of chondrocytes. Mechanically, Dmp1 (dentin matrix protein 1) was proved to be the downstream direct target of Mettl3 for m6A modification to regulate the differentiation of chondrocytes through bioinformatics analysis and correlated experiments. The Reader protein Ythdf1 mediated Dmp1 mRNA catalyzed by Mettl3. In vivo, the formation of subcutaneous ectopic cartilage-like tissue further supported the in vitro results. In conclusion, the gene regulation of Mettl3/m6A/Ythdf1/Dmp1 axis in hypertrophic differentiation of chondrocytes for the development of endochondral osteogenesis may supply a promising treatment strategy for the repair and regeneration of bone defects.


Assuntos
Condrócitos , Metiltransferases , Diferenciação Celular/genética , Condrócitos/metabolismo , Proteínas da Matriz Extracelular , Humanos , Hipertrofia , Metiltransferases/genética , Metiltransferases/metabolismo , Osteogênese/genética , Fosfoproteínas , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Proteínas de Ligação a RNA/metabolismo
9.
J Med Virol ; 83(4): 616-21, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21328375

RESUMO

The objectives of the study were to estimate the prevalence of anti-hepatitis C virus (HCV) positivity among blood donors from Chengdu, China, and to determine risk factors associated with infection. In this study, data were collected from volunteer blood donors between July 2006 and June 2007. Anti-HCV test was performed in 119,518 donors. To identify risk factors associated with HCV infections a case-control study was conducted in 305 unique HCV-seropositive blood donors and 610 seronegative donors matched for age and sex. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated using logistic regression. The population attributable risk (PAR) to risk factor was estimated according to the Bruzzi's formula. The prevalence of anti-HCV positivity was 0.53% (95% CI: 0.489-0.572%). The final multivariate model included the following independent HCV risk factors: razor sharing (OR=29.16; 95% CI: 12.89-66.00), blood transfusion (OR=20.84; 95% CI: 3.76-115.45), acupuncture (OR=8.01; 95% CI: 3.16-20.30), a history of hospitalization, injections >10 years earlier, a family history of hepatitis B, dental treatment, and ear piercing. The PAR of risk factors are 68.4%, 6.3%, 14.1%, 23.1%, 29.5%, 29.3%, 38.9%, and 27.8%, respectively, and the total PAR is 98.3%. Infection with HCV among blood donors in Chengdu is associated with iatrogenic risk factors and beauty treatment-related risk. Razor sharing is an important risk factor for HCV infection. These results indicate that infection control measures in healthcare settings may reduce the burden of HCV infection and there is a need for development of effective educational programs to improve HCV knowledge among beauty culture professionals, barber cosmetologists, and the general public to avoid risk behaviors.


Assuntos
Doadores de Sangue , Anticorpos Anti-Hepatite C/sangue , Hepatite C/epidemiologia , Hepatite C/virologia , Adolescente , Adulto , Estudos de Casos e Controles , China/epidemiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fatores de Risco , Estudos Soroepidemiológicos , Adulto Jovem
10.
J Phys Chem B ; 113(24): 8357-61, 2009 Jun 18.
Artigo em Inglês | MEDLINE | ID: mdl-19462938

RESUMO

Three kinds of conventional surfactants, namely, two nonionic surfactants [polyethylene glycol (23) lauryl ether (Brij-35) and Triton X-100 (TX-100)], one cationic surfactant [n-tetradecyltrimethyl ammonium bromide (TTAB)], and an anionic surfactant [sodium n-dodecyl sulfate (SDS)}, were mixed into the quaternary ammonium gemini surfactant [C(14)H(29)N(+)(CH(3))(2)](2)(CH(2))(2).2Br(-) (14-2-14) in aqueous solution. The exchange rate constants between 14-2-14 molecules in the mixed micelles and those in the bulk solution were detected using two nuclear magnetic resonance (NMR) methods: one-dimensional (1D) line shape analysis and two-dimensional (2D) exchange spectroscopy (EXSY). The results obtained from these two methods were consistent. Both showed that mixing a nonionic conventional surfactant, either Brij-35 or TX-100, enhanced the exchange process between the 14-2-14 molecules in the mixed micelles and those in the bulk solution. In contrast, the anionic surfactant SDS and the cationic surfactant TTAB slowed the process slightly.


Assuntos
Compostos de Amônio Quaternário/química , Tensoativos/química , Termodinâmica , Cátions/química , Espectroscopia de Ressonância Magnética/métodos , Micelas , Octoxinol/química , Polietilenoglicóis/química , Dodecilsulfato de Sódio/química , Soluções , Compostos de Trimetil Amônio/química
11.
Stem Cell Res Ther ; 10(1): 216, 2019 07 29.
Artigo em Inglês | MEDLINE | ID: mdl-31358056

RESUMO

OBJECTIVES: Temporomandibular joint osteoarthritis (TMJOA) is an inflammatory joint disease. This study investigated whether exosomes (Exos) of stem cells from human exfoliated deciduous teeth (SHEDs) have a therapeutic effect on TMJ inflammation and elucidated the underlying mechanisms. MATERIALS AND METHODS: SHEDs were verified by flow cytometry. SHED-Exos were identified by western blotting, nanoparticle tracking analysis, and transmission electron microscopy. Western blot and RT-qPCR were performed to verify the anti-inflammatory effects of SHED-Exos. MicroRNA (miRNA) array analysis was conducted to determine the miRNA expression profiles of SHED-Exos, and the key pathways were analyzed. After chondrocytes were treated with an miR-100-5p mimic or rapamycin, relative expression of genes was measured by RT-qPCR and western blotting. A luciferase reporter assay was performed to reveal the molecular role of the exosomal miR-100 target, mTOR. RESULTS: MiR-100-5p was enriched in the SHED-Exos. Treatment with SHED-Exos suppressed the expression of interleukin-6 (IL-6), IL-8, matrix metalloproteinase 1 (MMP1), MMP3, MMP9, MMP13, and disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5). Chondrocytes treated with the miR-100 mimic showed lower expression of MMP1, MMP9, MMP13, ADAMTS5, and mTOR. In contrast, miR-100 downregulation upregulated the MMPs and mTOR. Rapamycin treatment upregulated miR-100 and downregulated MMPs and ADAMTS5. Furthermore, the luciferase reporter assay demonstrated that miR-100-5p directly targeted the mTOR 3' untranslated region and that SHED-Exos miR-100-5p repressed mTOR expression. CONCLUSIONS: This study demonstrated that SHED-Exos suppress inflammation in TMJ chondrocytes and may thus be a novel therapeutic agent for TMJ inflammation.


Assuntos
Condrócitos/citologia , Exossomos/metabolismo , MicroRNAs/metabolismo , Células-Tronco/metabolismo , Articulação Temporomandibular/citologia , Dente Decíduo/citologia , Western Blotting , Criança , Pré-Escolar , Condrócitos/metabolismo , Condrócitos/fisiologia , Humanos , Inflamação/metabolismo , Inflamação/terapia , Microscopia Eletrônica de Transmissão , Osteoartrite/metabolismo , Osteoartrite/terapia
12.
Shanghai Kou Qiang Yi Xue ; 27(1): 22-24, 2018 Feb.
Artigo em Zh | MEDLINE | ID: mdl-29946635

RESUMO

PURPOSE: To observe the effect of overdose fluoride on the expression of TGF-ß3 in rat incisor and to explore the possible mechanism of dental fluorosis. METHODS: Twenty Wistar rats were randomly divided into 2 groups. The animals were maintained in standard environmental conditions with free access to food and water (control group) or water added with 100mg/L F (experimental group). The rats were killed at the end of 8th week. The expression of TGF-ß3 was detected by immunohistochemical staining. Statistical analysis was performed using SPSS12.0 software package. RESULTS: The expression of TGF-ß3 in ameloblasts was significantly inhibited in the experimental of group II(P<0.01). The gray value of the control group and the fluorine group were 85.89±7.90, 116.76±8.04, respectively. CONCLUSIONS: Fluoride might disturb the signal transduction between the epithelia and mesenchyma by inhibiting the expression of TGF-ß3 in ameloblasts, which in turn may inhibit the differentiation and function of the tooth-forming cells.


Assuntos
Fluoretos , Fluorose Dentária , Fator de Crescimento Transformador beta3 , Animais , Fluoretos/toxicidade , Incisivo/metabolismo , Ratos , Ratos Wistar , Fator de Crescimento Transformador beta3/efeitos dos fármacos , Fator de Crescimento Transformador beta3/metabolismo
13.
J Colloid Interface Sci ; 476: 158-166, 2016 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-27214146

RESUMO

Due to the high therapeutic efficiency and minimum damage towards normal tissues, phototherapy has drawn a great deal of attention in recent decades. Herein, we reported the synthesis of novel phosphopeptide-decorated magnetic nanoparticles (peptide-Fe3O4 nanoparticles), and their usages in photothermal therapy against solid tumor. By using a classical coprecipitation method and a facile ligand exchange route, these peptide-Fe3O4 nanoparticles were prepared with inexpensive inhesion. Upon the irradiation of a near-infrared (NIR) light, these nanoagents exhibited great photothermal effect with high photo-stability. In vitro biocompatibility studies of these peptide-Fe3O4 nanoparticles indicated their low cytotoxicity, negligible hemolysis, and no effect on blood coagulation. As expected, 4T1 murine breast cancer cells could be effectively damaged by these light-mediated nanoagents. Significantly, animal experiments demonstrated that these nanoagents held great solid tumor ablation effect with the assistance of a NIR laser irradiation. Additional studies focused on the long-term toxicity of these nanoagents indicated their high bio-compatibility. Thus, these peptide-Fe3O4 nanoparticles could bring more opportunities to a new generation of photothermal agents in the field of biomedicine.


Assuntos
Materiais Biocompatíveis/farmacologia , Bioengenharia , Nanopartículas de Magnetita/química , Neoplasias Experimentais/tratamento farmacológico , Fosfopeptídeos/química , Fosfopeptídeos/farmacologia , Fototerapia , Animais , Apoptose/efeitos dos fármacos , Materiais Biocompatíveis/síntese química , Materiais Biocompatíveis/química , Materiais Biocompatíveis/uso terapêutico , Sobrevivência Celular/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos BALB C , Neoplasias Experimentais/patologia , Tamanho da Partícula , Fosfopeptídeos/uso terapêutico , Propriedades de Superfície , Células Tumorais Cultivadas
14.
Colloids Surf B Biointerfaces ; 115: 164-9, 2014 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-24334269

RESUMO

mPEGylated artesunate prodrug was synthesized via esterification between poly(ethylene glycol) monomethyl ether (mPEG) and artesunate (ART). The product was inclined to form nanocapsules in aqueous media due to its amphiphilic nature. These nanocapsules showed narrow size distribution, with an average particle size of 88.7 nm measured by dynamic laser scattering (DLS). Their vesical morphology was further confirmed by transmission electron microscopy (TEM). We found that the release of ART from the nanocapsules was controllable, which was contributed to the easily hydrolyzed property of the ester bond. In addition, the cytotoxicity of the prodrug against L1210 and MCF7 cell lines showed an essential decrease compared with the free ART. These results present a new strategy in designing anti-tumor ART nanocapsules for targeting tumor cells.


Assuntos
Artemisininas/farmacologia , Nanocápsulas/química , Polietilenoglicóis/química , Pró-Fármacos/farmacologia , Animais , Artemisininas/síntese química , Artemisininas/química , Artesunato , Morte Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Humanos , Células MCF-7 , Espectroscopia de Ressonância Magnética , Camundongos , Micelas , Nanocápsulas/ultraestrutura , Tamanho da Partícula , Polietilenoglicóis/síntese química , Pró-Fármacos/síntese química , Pró-Fármacos/química , Espectroscopia de Infravermelho com Transformada de Fourier
15.
Colloids Surf B Biointerfaces ; 100: 138-45, 2012 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-22766290

RESUMO

Biodegradable micelles with sheddable poly(ethylene glycol) shells were fabricated based on poly(ethylene glycol)-block-poly(γ-benzyl L-glutamate) (mPEG-SS-PBLG) diblock copolymer and applied as the carrier of 7-ethyl-10-hydroxy-camptothecin (SN-38) in order to enhance its solubility and stability in aqueous media. The diblock polymer was designed to have the hydrophilic PEG moiety and hydrophobic PBLG moiety linked by biodegradable disulfide bond, so in reducing environment the PEG shells can be detached. The polymer was able to form the micelles of nano-scale in aqueous media, suggesting their passive targeting potential to tumor tissue. Water-insoluble antitumor drug, SN-38, was easily encapsulated into mPEG-SS-PBLG nanomicelles by lyophilization method. When setting theoretical drug loading content at 10 wt%, the drug encapsulation efficiency (EE) was assayed as 73.5%. Owing to the disulfide bond in mPEG-SS-PBLG, intense release of SN-38 occurred in the presence of dithiothreitol (DTT) at the concentration of simulating the intracellular condition, however, micelles showed gradual release of SN-38 in the absence of DTT. Also, the mPEG-SS-PBLG micelles effectively protected the active lactone ring of SN-38 from hydrolysis under physiological condition. Compared with free SN-38, SN-38-loaded nanomicelles showed essentially decreased cytotoxicity against L929 cell line in 24h, bare mPEG-SS-PBLG nanomicelles showed almost non-toxicity.


Assuntos
Antineoplásicos Fitogênicos/química , Materiais Biocompatíveis/síntese química , Camptotecina/análogos & derivados , Preparações de Ação Retardada/síntese química , Portadores de Fármacos/síntese química , Polietilenoglicóis/síntese química , Ácido Poliglutâmico/análogos & derivados , Animais , Antineoplásicos Fitogênicos/farmacologia , Camptotecina/química , Camptotecina/farmacologia , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Dissulfetos/química , Ditiotreitol/química , Composição de Medicamentos , Liofilização , Humanos , Interações Hidrofóbicas e Hidrofílicas , Irinotecano , Cinética , Camundongos , Micelas , Oxirredução , Polietilenoglicóis/química , Ácido Poliglutâmico/síntese química , Solubilidade , Água
16.
Vaccine ; 28(19): 3435-9, 2010 Apr 26.
Artigo em Inglês | MEDLINE | ID: mdl-20197135

RESUMO

To distinguish the antibodies induced by Foot-and-mouth disease virus (FMDV) infection from those induced by vaccination, a recombinant N-terminal truncated FMDV non-structural protein (NSP) of 3AB, designated as r3aB, was constructed by deleting 80 amino acids displayed about 30% homology to transposase IS4 family protein of Escherichia coli, expressed in E. coli BL21 (DE3) and then purified. The r3aB was majorly expressed in soluble fraction and presented as homogeneous monomers after purification. Using r3aB as coating antigen, an indirect ELISA was established to specifically identify antibodies induced by FMDV infection but not those induced by vaccination. Compared with 3AB, r3aB was more specific to catch antibodies against NSP. The performance of this assay was validated by two commercial FMDV NSP ELISA kits. The result suggested that the r3aB coated ELISA could be developed into a kit to better distinguish between infected and vaccinated cattle.


Assuntos
Doenças dos Bovinos/imunologia , Doenças dos Bovinos/prevenção & controle , Febre Aftosa/imunologia , Febre Aftosa/prevenção & controle , Proteínas não Estruturais Virais , Vacinas Virais/imunologia , Animais , Anticorpos Antivirais/sangue , Bovinos , Clonagem Molecular , Diagnóstico Diferencial , Ensaio de Imunoadsorção Enzimática/métodos , Escherichia coli/genética , Expressão Gênica , Proteínas Recombinantes/genética , Proteínas Recombinantes/isolamento & purificação , Sensibilidade e Especificidade , Deleção de Sequência , Proteínas não Estruturais Virais/genética , Proteínas não Estruturais Virais/isolamento & purificação
17.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 42(4): 242-4, 2007 Apr.
Artigo em Zh | MEDLINE | ID: mdl-17683676

RESUMO

OBJECTIVE: To investigate the effects of overdose fluoride on the expression of basic fibroblast growth factor (bFGF) in rat incisors. METHODS: Twenty Wistar rats were randomly divided into 2 groups: group I (control group) distilled water was given; group II (experimental group) 100 mg/L NaF was given. The rats were killed at the end of 8 th week. Immunohistochemical staining was used to study the expression of bFGF in rat incisors. RESULTS: Immunohistochemical results demonstrated the presence of bFGF in ameloblasts, odontoblasts of rat incisors. The expression of bFGF was reduced in group II (P < 0.01). CONCLUSIONS: Overdose fluoride inhibits the expression of bFGF and affects the interaction between dental epithelium and dental mesenchyme, which leads to the enamel demineralization.


Assuntos
Fator 2 de Crescimento de Fibroblastos/metabolismo , Fluorose Dentária/metabolismo , Incisivo/metabolismo , Animais , Modelos Animais de Doenças , Feminino , Masculino , Ratos , Ratos Wistar
19.
Biotechnol Bioeng ; 87(3): 364-75, 2004 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-15281111

RESUMO

Nanophased porous hydroxyapatite beads with particle diameters of 25 microm and 30 microm intended for use in protein and biomolecule separation are characterized with respect to chromatographic characteristics. These particles were produced from a hydroxyapatite gel by a controlled spray process yielding microspheres containing hydroxyapatite nanocrystals. By calcification of the microspheres, nanophased porous hydroxyapatite beads were obtained. As a reference material, ceramic hydroxyapatite Types I and II with a particle diameter of 40 microm was chosen. SEM pictures show that the surface of the nanophased hydroxyapatite is very rough compared to ceramic hydroxyapatite Types I and Type II. The calcium-to-phosphorous ratio of this nanophased hydroxyapatite is 1.6, which is slightly below the theoretical ratio of 1.67 of pure hydroxyapatite. The porosity is greater than 60%. An IgG binding capacity of 60.7 mg/ml for Bio-Rad Type I and 36.0 mg/ml for Type II, 42.0 mg/ml for the nanophased material with 25 microm and 19.7 mg/ml for the nanophased material with 30 microm were observed. The nanophased material with 30 microm had the lowest mass transfer resistancy as indicated by the dependency of the dynamic binding capacity on velocity. It is assumed that the mass transport properties are characterized by a low particle diffusion resistancy or by slight intraparticle convection. The material also showed high selectivity for IgG. When culture supernatant with 5% FCS containing 3 mg/ml was loaded, pure IgG could be eluted by linear gradient with increasing sodium phosphate concentration. This nanophased material comprises a novel stationary phase for IgG separation.


Assuntos
Cromatografia/métodos , Durapatita/química , Imunoglobulina G/isolamento & purificação , Nanotubos/química , Nanotubos/ultraestrutura , Adsorção , Materiais Biocompatíveis/química , Cromatografia/instrumentação , Humanos , Teste de Materiais , Tamanho da Partícula , Transição de Fase , Porosidade
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