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1.
Rheumatology (Oxford) ; 59(10): 3070-3080, 2020 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-32417913

RESUMO

OBJECTIVES: IL-37 has been identified as an important anti-inflammatory and immunosuppressive factor. This study was undertaken to explore how IL-37 affects M1/M2-like macrophage polarization and thus contributes to anti-inflammatory processes in the temporomandibular joint. METHODS: Western blotting, quantitative real-time PCR (qRT-PCR) and immunofluorescence were used to verify the IL-37-induced polarization shift from the M1 phenotype to the M2 phenotype, and the related key pathways were analysed by western blotting. Human chondrocytes were stimulated with M1-conditioned medium (CM) or IL-37-pretreated M1-CM, and inflammatory cytokines were detected. siRNA-IL-1R8 and MCC-950 were used to investigate the mechanism underlying the anti-inflammatory effects of IL-37. Complete Freund's adjuvant-induced and disc perforation-induced inflammation models were used for in vivo studies. Haematoxylin and eosin, immunohistochemical and safranin-O staining protocols were used to analyse histological changes in the synovium and condyle. RESULTS: Western blotting, qRT-PCR and immunofluorescence showed that IL-37 inhibited M1 marker expression and upregulated M2 marker expression. Western blotting and qRT-PCR showed that pretreatment with IL-37 suppressed inflammatory cytokine expression in chondrocytes. IL-37 inhibited the expression of NLRP3 and upregulated the expression of IL-1R8. Si-IL-1R8 and MCC-950 further confirmed that the anti-inflammatory properties of IL-37 were dependent on the presence of IL-1R8 and NLRP3. In vivo, IL-37 reduced synovial M1 marker expression and cartilage degeneration and increased M2 marker expression. CONCLUSION: IL-37 shifting of the polarization of macrophages from the pro-inflammatory M1 phenotype to the beneficial anti-inflammatory M2 phenotype seems to be a promising therapeutic strategy for treating temporomandibular joint inflammation.


Assuntos
Polaridade Celular/efeitos dos fármacos , Interleucina-1/farmacologia , Ativação de Macrófagos/efeitos dos fármacos , Macrófagos/efeitos dos fármacos , Proteína 3 que Contém Domínio de Pirina da Família NLR/metabolismo , Transtornos da Articulação Temporomandibular/terapia , Western Blotting , Condrócitos/efeitos dos fármacos , Condrócitos/metabolismo , Meios de Cultivo Condicionados/farmacologia , Citocinas/metabolismo , Adjuvante de Freund , Furanos , Compostos Heterocíclicos de 4 ou mais Anéis/farmacologia , Humanos , Indenos , Inflamassomos/efeitos dos fármacos , Macrófagos/metabolismo , Côndilo Mandibular/patologia , Osteoartrite/induzido quimicamente , Osteoartrite/metabolismo , Osteoartrite/patologia , Osteoartrite/terapia , RNA Interferente Pequeno/farmacologia , Reação em Cadeia da Polimerase em Tempo Real , Receptores de Interleucina-1/metabolismo , Sulfonamidas , Sulfonas/farmacologia , Membrana Sinovial/patologia , Transtornos da Articulação Temporomandibular/induzido quimicamente , Transtornos da Articulação Temporomandibular/metabolismo , Transtornos da Articulação Temporomandibular/patologia , Regulação para Cima
2.
Exp Brain Res ; 237(8): 2045-2059, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31165915

RESUMO

Common facial expressions of emotion have distinctive patterns of facial muscle movements that are culturally similar among humans, and perceiving these expressions is associated with stereotypical gaze allocation at local facial regions that are characteristic for each expression, such as eyes in angry faces. It is, however, unclear to what extent this 'universality' view can be extended to process heterospecific facial expressions, and how 'social learning' process contributes to heterospecific expression perception. In this eye-tracking study, we examined face-viewing gaze allocation of human (including dog owners and non-dog owners) and monkey observers while exploring expressive human, chimpanzee, monkey and dog faces (positive, neutral and negative expressions in human and dog faces; neutral and negative expressions in chimpanzee and monkey faces). Human observers showed species- and experience-dependent expression categorization accuracy. Furthermore, both human and monkey observers demonstrated different face-viewing gaze distributions which were also species dependent. Specifically, humans predominately attended at human eyes but animal mouth when judging facial expressions. Monkeys' gaze distributions in exploring human and monkey faces were qualitatively different from exploring chimpanzee and dog faces. Interestingly, the gaze behaviour of both human and monkey observers were further affected by their prior experience of the viewed species. It seems that facial expression processing is species dependent, and social learning may play a significant role in discriminating even rudimentary types of heterospecific expressions.


Assuntos
Movimentos Oculares/fisiologia , Expressão Facial , Fixação Ocular/fisiologia , Reconhecimento Visual de Modelos/fisiologia , Estimulação Luminosa/métodos , Adolescente , Adulto , Animais , Cães , Feminino , Haplorrinos , Humanos , Macaca , Masculino , Pan troglodytes , Especificidade da Espécie , Adulto Jovem
3.
Int J Biol Macromol ; 224: 143-155, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36257360

RESUMO

In this paper, ternary DES (choline chloride, glycerol, Lewis acid) was used to pretreat lignocellulose, and the DES solution with dissolved lignin was utilized as the medium of hydrogel to prepare DES-based polyacrylic acid/polyvinyl alcohol (PAA/PVA) double network hydrogels with great mechanical properties, self-adhesion, and high electrochemical sensitivity. The entanglement of PAA with PVA chains, the covalent linkage between Al3+ and PAA chains and the metal phenol network (MPN) formed by Al3+ and lignin improved the mechanical properties of the hydrogels, enabling the prepared hydrogels to achieve a tensile strain of 400 % and an elongation at break of 150 kPa. Secondly, the introduction of DES solution endowed the hydrogel with excellent electrical sensing ability and anti-freezing property, so that the hydrogel still maintains good flexibility and ionic conductivity at -20 °C. It was also found that the above hydrogel can achieve a high gauge factor of 4.19 as a flexible sensor, which provides scientific ideas for the application of the pretreated DES solution in the field of flexible wearable.


Assuntos
Solventes Eutéticos Profundos , Hidrogéis , Lignina , Condutividade Elétrica , Íons
4.
Int J Biol Macromol ; 203: 49-57, 2022 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-35038472

RESUMO

The study presents the preparation of novel biocomposites based on different lignin fractions and polylactic acid (PLA). Based on the improvement of PLA mechanical properties, we proposed a process to assemble a series of lignin samples and PLA matrix using melt blending to investigate the effects on PLA properties from the perspective of the structure and molecular weight for lignin. The lignin was extracted from pine residue using deep eutectic solvent (DES) and was subjected to fractionation with ethanol and acetone as well as esterification modification with succinic anhydride (SAn). The treated lignin samples were used as additives, which not only improved the mechanical properties of PLA on the basis of retaining its thermal stability, but also granted excellent antimicrobial and biocompatibility properties. The results showed that the molecular weight of lignin was negatively correlated with the phenolic hydroxyl content, and the mechanical properties of the composites were also significantly affected by different molecular weights of lignin, indicating that the inhomogeneity of lignin affects its value-added utilization. The enhanced mechanical properties, antibacterial effect, and steady biocompatibility provide potential possibilities for lignin-based composites in biomedical applications.


Assuntos
Lignina , Poliésteres , Fracionamento Químico , Esterificação , Lignina/química , Poliésteres/química
5.
World J Clin Cases ; 9(36): 11425-11436, 2021 Dec 26.
Artigo em Inglês | MEDLINE | ID: mdl-35071574

RESUMO

BACKGROUND: Root canal retreatment is common after root canal therapy in clinical situations. Especially, completing the retreatment of variant root canals can be challenging. This is particularly true for the molars located at the end of the dental arch. However, advancements in digital dental diagnosis and treatment techniques can solve these problems. Here, we describe a case of a maxillary second molar with a variant distobuccal root canal treated via a novel "inlay-guided endodontics" technique based on improved computer-generated programs. CASE SUMMARY: A 63-year-old man complained of a defect in the maxillary left second molar. The tooth, diagnosed with post-treatment endodontic disease, was initially treated by conventional methods, which were ineffective. Our "inlay-guided endodontics" technique was subsequently adopted, with the establishment of a precise integrated three-dimensional (3D) plate model of cone-beam computed tomography data and a digital impression of the dentition. An optimal root canal approach was generated for the "virtual file" in the 3D model. The plate data were imported into a 3D printer and printed. With the help of the guide plate, the file was accurately placed into the cervical third of the distal root canal. The root canal and prosthodontic treatments successfully proceeded subsequently. CONCLUSION: Our newly developed inlay guide plates may facilitate individualized and minimally invasive root canal treatment.

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