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1.
Environ Monit Assess ; 196(5): 430, 2024 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-38578570

RESUMO

Arsenic contamination in soils poses a critical global challenge, yet the influence of surfactants on arsenic adsorption behavior is often underestimated. This study aims to investigate the effects of three representative surfactants, namely cetyltrimethylammonium bromide (CTAB), sodium dodecyl sulfate (SDS), and polyethylene glycol anhydrous sugar alcohol monooleate (Tween 80), on arsenic adsorption behavior in soils. The adsorption isotherm shifts from a single Temkin model without surfactants to both the Langmuir and Temkin models in the presence of surfactants, indicating the simultaneous occurrence of monolayer and multilayer adsorption for arsenic in soils. Moreover, the surfactants can inhibit the adsorption and hasten the attainment of adsorption equilibrium. SDS displayed the most inhibitory effect on arsenic adsorption, followed by Tween 80 and CTAB, due to the competitive adsorption, electrostatic interaction, and hydrophobic interaction. Variations in zeta potential with different surfactants further elucidate this inhibitory phenomenon. Through orthogonal experiment analyses, pH emerges as a primary factor influencing arsenic adsorption in soils, with surfactant concentration and type identified as secondary factors. Temperature notably affects CTAB, with the adsorption inhibition rate plummeting to a mere 0.88% at 50 °C. Sequential extraction analysis revealed that surfactants enhanced the bioavailability of arsenic. The FTIR, XRD, SEM, and CA analyses further support the mechanism underlying the effect of surfactants on arsenic adsorption in soil. These analyses indicate that surfactants modify the composition and abundance of functional groups, hinder the formation of arsenic-containing substances, and improve soil compactness, smoothness, and hydrophilicity. This study provides valuable insights into the effect of surfactants in arsenic-contaminated soils, which is often ignored in previous work.


Assuntos
Arsênio , Tensoativos , Tensoativos/química , Solo/química , Polissorbatos , Cetrimônio , Adsorção , Arsênio/química , Monitoramento Ambiental
2.
Environ Sci Technol ; 57(2): 1167-1176, 2023 01 17.
Artigo em Inglês | MEDLINE | ID: mdl-36599128

RESUMO

Microplastics are readily accumulated in coastal sediments, where active sulfur (S) cycling takes place. However, the effects of microplastics on S cycling in coastal sediments and their underlying mechanisms remain poorly understood. In this study, the transformation patterns of different S species in mangrove sediments amended with different microplastics and their associated microbial communities were investigated using stable isotopic analysis and metagenomic sequencing. Biodegradable poly(lactic acid) (PLA) microplastics treatment increased sulfate (SO42-) reduction to yield more acid-volatile S and elementary S, which were subsequently transformed to chromium-reducible S (CRS). The S isotope fractionation between SO42- and CRS in PLA treatment increased by 9.1‰ from days 0 to 20, which was greater than 6.8‰ in the control. In contrast, recalcitrant petroleum-based poly(ethylene terephthalate) (PET) and polyvinyl chloride (PVC) microplastics had less impact on the sulfate reduction, resulting in 7.6 and 7.7‰ of S isotope fractionation between SO42- and CRS from days 0 to 20, respectively. The pronounced S isotope fractionation in PLA treatment was associated with increased relative abundance of Desulfovibrio-related sulfate-reducing bacteria, which contributed a large proportion of the microbial genes responsible for dissimilatory sulfate reduction. Overall, these findings provide insights into the potential impacts of microplastics exposure on the biogeochemical S cycle in coastal sediments.


Assuntos
Microplásticos , Plásticos , Isótopos de Enxofre/análise , Enxofre , Isótopos/análise , Poliésteres , Sulfatos/análise , Sedimentos Geológicos/análise
3.
Lasers Med Sci ; 37(3): 1531-1537, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-34647190

RESUMO

There are few studies on excimer laser (308 nm) atherectomy in the treatment of infrapopliteal artery disease. The purpose of this retrospective clinical study was to assess the efficacy and safety of excimer laser atherectomy (ELA) in combination with adjuvant drug-coated balloon angioplasty (DCB) compared to DCB for infrapopliteal arterial revascularization in patients with ischemic diabetic foot. From September 2018 to February 2019, a total of 79 patients with diabetic foot were treated for infrapopliteal arterial revascularization at Tianjin First Central Hospital (Tianjin, China). In this project, 35 patients were treated with ELA combined with DCB angioplasty, and 44 patients were treated with DCB angioplasty. The patients' baseline characteristics were similar between the 2 groups. The primary efficacy endpoints through 24 months were clinically driven target lesion revascularization (CD-TLR), wound healing rate, major amputation rate, and target vessel patency rate. The primary safety endpoint through 24 months was all-cause mortality. The primary efficacy results at 24 months of ELA + DCB versus DCB were CD-TLR of 14.3% versus 34.1% (p = 0.044), wound healing rate of 88.6% versus 65.9% (p = 0.019), target vessel patency rate of 80.0% versus 52.3% (p = 0.010), and major amputations rate of 5.7% versus 22.7% (p = 0.036). The safety signal at 24 months of all-cause mortality rate was 2.9% for ELA + DCB group and 4.5% for DCB group (p = 0.957). ELA combined with DCB angioplasty is more effective than DCB in the treatment of infrapopliteal artery disease in patients with ischemic diabetic foot, which can improve the wound healing rate and target vessel patency rate. There was no statistical difference in the safety results between the two groups.


Assuntos
Angioplastia com Balão , Diabetes Mellitus , Pé Diabético , Doença Arterial Periférica , Angioplastia com Balão/efeitos adversos , Aterectomia/efeitos adversos , Aterectomia/métodos , Materiais Revestidos Biocompatíveis , Pé Diabético/terapia , Artéria Femoral , Humanos , Lasers de Excimer/uso terapêutico , Doença Arterial Periférica/cirurgia , Artéria Poplítea/cirurgia , Estudos Retrospectivos , Resultado do Tratamento , Grau de Desobstrução Vascular
4.
Prep Biochem Biotechnol ; 52(6): 611-617, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-34550864

RESUMO

We previously reported an in vitro enzymatic pathway for conversion of nonfood cellulose to starch (PNAS,110 (18): 7182-7187, 2013), in which the two sequential enzymes cellobiose phosphorylase (CBP) from Clostridium thermocellum and potato alpha-glucan phosphorylase (PGP) from Solanum tuberosum were the two key enzymes responsible for the whole conversion rate. In this work CBP and PGP were fused to form a large enzyme and it turned out that the fusion protein could exhibit a good bifunctionality when PGP moiety was put at the N-terminus and CBP moiety at the C-terminus (designated as PGP-CBP). Although the coupled reaction rate of PGP-CBP was decreased by 23.0% compared with the free enzymes, substrate channeling between the two active sites in PGP-CBP was formed, demonstrated by the introduction of the competing enzyme of PGP to the reaction system. The potential of PGP-CBP fusion enzyme being applied to the conversion of cellulose to amylose was discussed.


Assuntos
Celobiose , Solanum tuberosum , Celobiose/metabolismo , Celulose/metabolismo , Glucosiltransferases , Fosforilases/química , Fosforilases/genética , Solanum tuberosum/metabolismo , Amido
5.
Int J Mol Sci ; 22(22)2021 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-34830066

RESUMO

Bacterial canker caused by Pseudomonas syringae pv. actinidiae (Psa) has led to considerable losses in all major kiwifruit-growing areas. There are no commercial products in the market to effectively control this disease. Therefore, the defense resistance of host plants is a prospective option. In our previous study, sulfur could improve the resistance of kiwifruit to Psa infection. However, the mechanisms of inducing resistance remain largely unclear. In this study, disease severity and protection efficiency were tested after applying sulfur, with different concentrations in the field. The results indicated that sulfur could reduce the disease index by 30.26 and 31.6 and recorded high protection efficiency of 76.67% and 77.00% after one and two years, respectively, when the concentration of induction treatments was 2.0 kg/m3. Ultrastructural changes in kiwifruit stems after induction were demonstrated by scanning electron microscopy (SEM) and transmission electron microscopy (TEM), and the activities of phenylalanine ammonia-lyase (PAL), peroxidase (POD) and polyphenol oxidase (PPO), and the accumulation of lignin were determined by biochemical analyses. Our results showed that the morphological characteristics of trichomes and lenticels of kiwifruit stem were in the best defensive state respectively when the sulfur concentration was 3.0 kg/m3 and 1.5 kg/m3. Meanwhile, in the range of 0.5 to 2.0 kg/m3, the sulfur could promote the chloroplast and mitochondria of kiwifruit stems infected with Psa to gradually return to health status, increasing the thickness of the cell wall. In addition, sulfur increased the activities of PAL, POD and PPO, and promoted the accumulation of lignin in kiwifruit stems. Moreover, the sulfur protection efficiency was positively correlated with PPO activity (p < 0.05) and lignin content (p < 0.01), which revealed that the synergistic effect of protective enzyme activity and the phenolic metabolism pathway was the physiological effect of sulfur-induced kiwifruit resistance to Psa. This evidence highlights the importance of lignin content in kiwifruit stems as a defense mechanism in sulfur-induced resistance. These results suggest that sulfur enhances kiwifruit canker resistance via an increase in phenolic components and morphology structure modification in the kiwifruit stems. Therefore, this study could provide insights into sulfur to control kiwifruit canker caused by Psa.


Assuntos
Actinidia/efeitos dos fármacos , Actinidia/microbiologia , Fenóis/metabolismo , Doenças das Plantas/microbiologia , Doenças das Plantas/prevenção & controle , Pseudomonas syringae/efeitos dos fármacos , Enxofre/farmacologia , Actinidia/anatomia & histologia , Catecol Oxidase/metabolismo , Correlação de Dados , Lignina/metabolismo , Peroxidase/metabolismo , Fenilalanina Amônia-Liase/metabolismo , Caules de Planta/anatomia & histologia , Caules de Planta/efeitos dos fármacos , Caules de Planta/microbiologia , Caules de Planta/ultraestrutura , Infecções por Pseudomonas/tratamento farmacológico , Enxofre/uso terapêutico , Tricomas/anatomia & histologia , Tricomas/efeitos dos fármacos , Tricomas/microbiologia
6.
Chin J Traumatol ; 24(1): 57-62, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31757743

RESUMO

Two cases of type Ⅱ odontoid fractures were reported to share our experience in surgery treatment of such cases. A 33-year-old woman with comminuted type Ⅱ odontoid fracture and a 42-year-old man with fracture end hardened type Ⅱ odontoid fracture received surgical treatment in our hospital. Though imaging examination suggested that these two patients were suitable for anterior screw fixation, we encountered difficulties during the operation. The two patients eventually underwent posterior C1-C2 fusion surgery and recovered well. According to the experience of these two cases, we found that the fracture line angle and the degree of comminution are two important factors affecting surgical decision-making. Although anterior screw fixation is the ideal choice for type Ⅱ odontoid fractures with anterior superior to posterior inferior fracture line, it may not be the best choice for comminuted or fracture end hardened type Ⅱ odontoid fractures.


Assuntos
Fixação Interna de Fraturas/métodos , Fraturas Cominutivas/cirurgia , Processo Odontoide/lesões , Processo Odontoide/cirurgia , Fraturas da Coluna Vertebral/cirurgia , Fusão Vertebral/métodos , Adulto , Parafusos Ósseos , Tomada de Decisões , Feminino , Fraturas Cominutivas/diagnóstico por imagem , Fraturas Cominutivas/patologia , Humanos , Masculino , Processo Odontoide/diagnóstico por imagem , Processo Odontoide/patologia , Radiografia , Fraturas da Coluna Vertebral/classificação , Fraturas da Coluna Vertebral/diagnóstico por imagem , Fraturas da Coluna Vertebral/patologia , Tomografia Computadorizada por Raios X
7.
Environ Res ; 183: 109214, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-32044572

RESUMO

Uranium high-efficiency separation from seawater still has some obstacles such as slow sorption rate, poor selectivity and biofouling. Herein, we report a strategy for ultrafast and highly selective uranium extraction from seawater by positively charged conjugated microporous polymers (CMPs). The polymers are synthesized by Sonogashira-Hagihara cross-coupling reaction of 1,3-dibromo-5,5-dimethylhydantoin and 1,3,5-triethynylbenzene, and then modified with oxime and carboxyl via click reaction. The CMPs show an ultrafast sorption (0.46 mg g-1 day-1) for uranium, and possess an outstanding selectivity with a high sorption capacity ratio of U/V (8.4) in real seawater. The study of adsorption process and mechanism indicate that the CMPs skeleton exhibits high affinity for uranium and can accelerate the sorption, and uranium(VI) is adsorbed on the materials by the interaction of oxime/carboxyl ligands and hydantoin. Moreover, the material can be simply loaded onto the filter membrane, and shows remarkable antibiofouling properties against E. coli and S. aureus and excellent uptake capacity for uranium with low concentration in real seawater. This work may provide a promising approach to design adsorbents with fast adsorption rate, high selectivity and antibacterial activity, and expand the thinking over the development of novel and highly efficient adsorbents for uranium extraction from seawater.


Assuntos
Incrustação Biológica , Polímeros , Urânio , Incrustação Biológica/prevenção & controle , Escherichia coli , Água do Mar , Staphylococcus aureus
9.
Soft Matter ; 11(42): 8253-61, 2015 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-26350404

RESUMO

Most existing magnetic hydrogels are weak and brittle. The development of strong and tough magnetic hydrogels would extend their applications into uncultivated areas, such as in actuators for soft machines and guided catheters for magnetic navigation systems, which is still a big challenge. Here a facile and versatile approach to fabricating highly stretchable, exceptionally tough and notch-insensitive magnetic hydrogels, Fe(3)O(4)@Fe-alginate/polyacrylamide (PAAm), is developed, by dispersing alginate-coated Fe(3)O(4) nanoparticles into the interpenetrating polymer networks of alginate and PAAm, with hybrid physical and chemical crosslinks. A cantilever bending beam actuator as well as a proof-of-concept magnetically guided hydrogel catheter is demonstrated. The method proposed in this work can be integrated into other strong and tough magnetic hydrogels for the development of novel hydrogel nanocomposites with both desirable functionality and superior mechanical properties.


Assuntos
Resinas Acrílicas , Alginatos , Compostos Férricos , Hidrogéis/química , Nanopartículas de Magnetita/química , Ácido Glucurônico , Dureza , Ácidos Hexurônicos , Resistência ao Cisalhamento
10.
J Nanosci Nanotechnol ; 13(1): 409-16, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-23646747

RESUMO

In this work, a novel environmental-friendly waterborne polyurethane/ZnAl-layered double hydroxides/ZnO nanoparticles composite (WPU/ZnAl-LDHs/ZnO) was synthesized via in-situ polymerization. ZnAl-LDHs and ZnAl-LDHs/ZnO were synthesized by refluxing in an oil bath. In order to disperse ZnAl-LDHs/ZnO homogeneously into WPU matrix, ZnAl-LDHs/ZnO was firstly functionalized by isophorone diisocyanate. The incorporated content of ZnAl-LDHs/ZnO in the composite has profound effect on such physical properties as mechanical strength, thermal stability and water swelling. It is demonstrated that appropriate amount of ZnAl-LDHs/ZnO with good dispersion in the WPU matrix significantly improves the physical performance of the composites. Finally, the antibacterial activity of the composite was tested against G(-) Escherichia coli and G(+) Staphylococcus aureus. The results indicate that WPU incorporated with ZnAl-LDHs/ZnO shows strong antibacterial activity upon contact.


Assuntos
Hidróxido de Alumínio/administração & dosagem , Fenômenos Fisiológicos Bacterianos/efeitos dos fármacos , Nanopartículas/administração & dosagem , Nanopartículas/química , Poliuretanos/química , Óxido de Zinco/administração & dosagem , Hidróxido de Alumínio/química , Antibacterianos/química , Antibacterianos/efeitos da radiação , Sobrevivência Celular/efeitos dos fármacos , Teste de Materiais , Água/química , Óxido de Zinco/química
11.
Front Neurol ; 13: 1073607, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36588911

RESUMO

Background: Reactivation of latent varicella-zoster virus (VZV) can induce herpes zoster (HZ). Ramsay Hunt syndrome (RHS) occurs through the reactivation and proliferation of VZV in the geniculate ganglion, which can lead to vesicular rash in the ear or oral mucosa, accompanied by neurological disorders. Materials and methods: A 50-year-old man sought a remedy for pain in the right ear and face. Within 1 week, all his lower right teeth fell out, and in the following 3 months, his lower right mandibular alveolar bone gradually became necrotic. In the past 20 days, he experienced blister rash, hearing and taste loss, and slight facial paralysis. Results: After ruling out tumors and other infectious diseases, he was diagnosed with trigeminal HZ and RHS. Conclusion: Ramsay Hunt syndrome with tooth loss and alveolar osteonecrosis is rare. It requires long-term treatment of pain, and prevention and treatment of tooth loss and alveolar bone necrosis are difficult and warrant further study.

12.
J Hazard Mater ; 434: 128898, 2022 07 15.
Artigo em Inglês | MEDLINE | ID: mdl-35460994

RESUMO

Inspired by Namib Desert beetle's back which is patterned with different wetting properties, hydrophobic porous polystyrene microspheres embedded with hydrophilic surface micro-regions (HPHs) were designed and fabricated by the radical copolymerization in the W1/O/W2 double Pickering emulsions with high internal water phase. The synergistic effect of the hydrophobic surface and the hydrophilic surface micro-regions results in HPHs exhibiting superior performances for separating both surfactant-free and surfactant-stabilized O/W emulsions. After 60 s hand-shaking, the oil was absorbed and stored within HPHs which could be separated from the water using a 600-mesh sieve, and the TOC values of purified water could be reduced to 2.06 ± 0.06-67.38 ± 2.02 ppm when the initial oil content was 1 vol%. Meanwhile, HPHs could be recovered and reused through a simple treatment. The excellent oil removal efficiency was kept even after 50 cycles. High oil removal efficiency, general applicability, easy operation and excellent recyclability endow HPHs with great potential for practical applications. And this work provides a facile and general way to prepare porous polymer microspheres with wettability contrast surfaces.


Assuntos
Poliestirenos , Água , Emulsões/química , Microesferas , Porosidade , Água/química
13.
J Biomed Mater Res B Appl Biomater ; 110(4): 755-767, 2022 04.
Artigo em Inglês | MEDLINE | ID: mdl-34637601

RESUMO

Human-treated dentin matrix (hTDM) is a biomaterial scaffold, which can induce implant cells to differentiate into odontoblasts and then form neo-dentin. However, hTDM with long storage or prepared by high-speed handpiece would not to form neo-dentin. In this research, we developed two fresh hTDM with different grinding speeds, which were low-speed hTDM (LTDM) with maximum speed of 500 rpm and high-speed hTDM (HTDM) with a speed of 3,80,000 rpm. Here, we aim to understand whether there were induced regeneration capacity differences between LTDM and HTDM. Scanning electron microscope showed that DFCs grew well on both materials, but the morphology of DFCs and the extracellular matrix was different. Especially, the secreted extracellular matrixes on the inner surface of LTDM were regular morphology and ordered arrangement around the dentin tubules. The transcription-quantitative polymerase chain reaction (qRT-PCR), western blot and immunofluorescence assay showed that the dentin markers DSPP and DMP-1 were about 2× greater in DFCs induced by LTDM than by HTDM, and osteogenic marker BSP was about 2× greater in DFCs induced by HTDM than by LTDM. Histological examinations of the harvested grafts observed the formation of neo-tissue were different, and there were neo-dentin formed on the inner surface of LTDM and neo-cementum formed on the outer surface of HTDM. In summary, it found that the induction abilities of LTDM and HTDM are different, and the dentin matrix is directional. This study lays a necessary foundation for searching the key factors of dentin regeneration in future.


Assuntos
Dentina , Matriz Extracelular , Diferenciação Celular , Células Cultivadas , Humanos , Odontoblastos , Regeneração
14.
J Orthop Surg Res ; 16(1): 74, 2021 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-33478541

RESUMO

BACKGROUND: Mandibular fracture healing is a complex process involving nerves and growth factors. Nerve growth factor (NGF) not only facilitates the maintenance of sympathetic neurite growth but also stimulates other growth factors that can promote the essential osteogenesis and angiogenesis for fracture healing. Therefore, it is necessary to analyze the combined effects of NGF, bone morphogenic protein-9 (BMP-9), and vascular endothelial growth factor (VEGF) to accelerate the healing of mandible fractures. METHODS: The models of mandible fracture with local nerve injury established in 48 rabbits were randomly divided into nerve growth factor group (NGF group), gelatin sponge group (GS group), blank group, and intact group. The recovery of nerve reflex was assessed by observing the number of rabbits with lower lip responses to acupuncture. The fracture healing was observed with visual and CBCT, and then callus tissues from the mandibular fracture area were collected for hematoxylin and eosin (HE) staining observation, and the expression of BMP-9 and VEGF in callus at different stages was detected by quantitative real-time PCR (qRT-PCR). RESULTS: Needling reaction in the lower lip showed the number of animals with nerve reflex recovery was significantly higher in the NGF group than that in the GS and blank groups at the 2nd and 4th weeks after the operation. The combined results of macroscopic observation, CBCT examination, and histological analysis showed that a large number of osteoblasts and some vascular endothelial cells were found around the trabecular bone in the NGF group and the amount of callus formation and reconstruction was better than that in the GS group at the 2nd week after the operation. The qRT-PCR results indicated that the expression levels of BMP-9 and VEGF in the four groups reached the highest values at the 2nd week, while the expression levels of both in the NGF group were significantly higher than that in the GS group. CONCLUSION: The exogenous NGF could accelerate the healing of mandible fractures. This work will provide a new foundation and theoretical basis for clarifying the mechanism of fracture healing, thereby promoting fracture healing and reducing the disability rate of patients.


Assuntos
Consolidação da Fratura/genética , Expressão Gênica/efeitos dos fármacos , Fator 2 de Diferenciação de Crescimento/genética , Fator 2 de Diferenciação de Crescimento/metabolismo , Mandíbula/inervação , Traumatismos Mandibulares/genética , Traumatismos Mandibulares/fisiopatologia , Fator de Crescimento Neural/farmacologia , Traumatismos dos Nervos Periféricos/genética , Fator A de Crescimento do Endotélio Vascular/genética , Fator A de Crescimento do Endotélio Vascular/metabolismo , Animais , Modelos Animais de Doenças , Feminino , Consolidação da Fratura/fisiologia , Masculino , Neovascularização Fisiológica/efeitos dos fármacos , Neovascularização Fisiológica/genética , Osteogênese/efeitos dos fármacos , Osteogênese/genética , Coelhos , Estimulação Química
15.
Bioresour Technol ; 326: 124777, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33540214

RESUMO

To investigate the effect of microplastics (MPs) particles in vermicomposting, polyethylene (PE) particles added into sludge. Results showed that the vermicomposting with high MPs addition obtained lower removal efficiencies for organics than the vermicomposting with low MPs addition. The content of DOC and NH4+-N in M4 reactor (with the highest MPs addition) at 80 days was 8.4 mg/kg and 74.2 mg/kg, respectively. The pH, C/N, electrical conductivity (EC), and germination index (GI) results showed that the addition amount of MPs was directly proportional to the negative effect of composting. The negative effect mainly occurred after 20 days of composting. High MPs addition resulted in apparent oxidative stress and neurotoxicity on earthworm, the values of catalase (CAT) and acetylcholine esterase (AChE) in M4 reactor increased by 2.03 times and 1.60 times. The bacteria in M4 were more barren and lower in terms of diversity.


Assuntos
Compostagem , Oligoquetos , Animais , Microplásticos , Plásticos , Esgotos , Solo
16.
In Vitro Cell Dev Biol Anim ; 57(6): 620-630, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-34212339

RESUMO

Human dental follicle cells (HDFCs) are an ideal cell source of stem cells for dental tissue repair and regeneration and they have great potential for regenerative medicine applications. However, the conventional monolayer culture usually reduces cell proliferation and differentiation potential due to the continuous passage during in vitro expansion. In this study, primary HDFC spheroids were generated on 1% agarose, and the HDFCs spontaneously formed cell spheroids in the agarose-coated dishes. Compared with monolayer culture, the spheroid-derived HDFCs exhibited increased proliferative ability for later passage HDFCs as analysed by Cell Counting Kit-8 (CCK-8). The transcription-quantitative polymerase chain reaction (qRT-PCR), western blot and immunofluorescence assay showed that the expression of stemness marker genes Sox2, Oct4 and Nanog was increased significantly in the HDFC spheroids. Furthermore, we found that the odontogenic differentiation capability of HDFCs was significantly improved by spheroid culture in the agarose-coated dishes. On the other hand, the osteogenic differentiation capability was weakened compared with monolayer culture. Our results suggest that spheroid formation of HDFCs in agarose-coated dishes partially restores the proliferative ability of HDFCs at later passages, enhances their stemness and improves odontogenic differentiation capability in vitro. Therefore, spheroid formation of HDFCs has great therapeutic potential for stem cell clinical therapy.


Assuntos
Técnicas de Cultura de Células , Saco Dentário/crescimento & desenvolvimento , Odontogênese/efeitos dos fármacos , Esferoides Celulares/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacos , Diferenciação Celular/genética , Proliferação de Células/efeitos dos fármacos , Saco Dentário/citologia , Saco Dentário/metabolismo , Humanos , Odontogênese/genética , Sefarose/farmacologia , Esferoides Celulares/citologia , Células-Tronco/efeitos dos fármacos
17.
Nat Nanotechnol ; 15(11): 908-913, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-32868919

RESUMO

Optical tweezers have emerged as a powerful tool for the non-invasive trapping and manipulation of colloidal particles and biological cells1,2. However, the diffraction limit precludes the low-power trapping of nanometre-scale objects. Substantially increasing the laser power can provide enough trapping potential depth to trap nanoscale objects. Unfortunately, the substantial optical intensity required causes photo-toxicity and thermal stress in the trapped biological specimens3. Low-power near-field nano-optical tweezers comprising plasmonic nanoantennas and photonic crystal cavities have been explored for stable nanoscale object trapping4-13. However, the demonstrated approaches still require that the object is trapped at the high-light-intensity region. We report a new kind of optically controlled nanotweezers, called opto-thermo-electrohydrodynamic tweezers, that enable the trapping and dynamic manipulation of nanometre-scale objects at locations that are several micrometres away from the high-intensity laser focus. At the trapping locations, the nanoscale objects experience both negligible photothermal heating and light intensity. Opto-thermo-electrohydrodynamic tweezers employ a finite array of plasmonic nanoholes illuminated with light and an applied a.c. electric field to create the spatially varying electrohydrodynamic potential that can rapidly trap sub-10 nm biomolecules at femtomolar concentrations on demand. This non-invasive optical nanotweezing approach is expected to open new opportunities in nanoscience and life science by offering an unprecedented level of control of nano-sized objects, including photo-sensitive biological molecules.


Assuntos
Nanoestruturas/ultraestrutura , Pinças Ópticas , Animais , Bovinos , Eletricidade , Hidrodinâmica , Nanoestruturas/química , Nanotecnologia/instrumentação , Tamanho da Partícula , Fótons , Poliestirenos/análise , Soroalbumina Bovina/análise , Temperatura
18.
Nanoscale ; 12(38): 19665-19672, 2020 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-32966502

RESUMO

Photothermal therapy (PTT), a powerful tool for non-invasive cancer treatment, has been recognized as an alternative strategy for cancer therapy in the clinic, and it is promoted by optical absorbing agents (photothermal agents) that can intensively convert near-infrared (NIR) light into thermal energy for cancer ablation. Conjugated polymer nanoparticles (CPNs) have recently attracted extensive attention owing to their excellent photothermal properties. However, the absorption of typical CPNs is mostly located in the traditional near-infrared region (NIR-I, 700-900 nm), which suffers from low tissue penetration, so the penetration depth is still limited and severely restricts their further applications. Compared with the NIR-I light, the second near-infrared window light (NIR-II, 1000-1700 nm) could efficiently enhance the tissue penetration depth, however, CPNs which absorb NIR-II region light are still especially limited and need further exploration. Here, a thieno-isoindigo derivative-based Donor-Acceptor (D-A) polymer (BTPBFDTS), which exhibited excellent absorption characteristics from the NIR-I to NIR-II window, was prepared. After formation of nanoparticles and surface functionalization, the prepared nanoparticles (NPsBTPBFDTS@HA NPs) exhibited obvious targeting ability, high photothermal conversion efficiency and photoacoustic imaging effects under 1064 nm irradiation. Both in vitro and in vivo studies demonstrate that our obtained NPsBTPBFDTS@HA nanoparticles possess excellent PTT efficacy including extremely high cancer cell killing ability and admirable tumor elimination efficiency. Hence, this work developed a promising photothermal conversion agent based on CPNs for cancer ablation.


Assuntos
Nanopartículas , Neoplasias , Humanos , Raios Infravermelhos , Neoplasias/diagnóstico por imagem , Neoplasias/tratamento farmacológico , Fototerapia , Terapia Fototérmica , Polímeros
19.
J Cancer Res Ther ; 16(7): 1714-1717, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33565522

RESUMO

Humerus is the long bone second-most commonly affected by metastases. An impending pathologic humeral fracture requires rigid surgical stabilization to prevent it from fully fracturing. In the present study, we report a case of a 71-year-old male patient with a visual analog score of 10 (indicating extreme pain) and an impending pathologic fracture that allowed for combined treatment with percutaneous microwave ablation and simultaneous injection of bone cement during Kirschner wire insertion. The procedure was performed successfully with no complications. The patient reported pain relief and improved quality of life and functional status 1 day, 7 days, 1 month, and 2 months after his procedure.


Assuntos
Adenocarcinoma/complicações , Neoplasias Ósseas/complicações , Dor do Câncer/terapia , Fraturas Espontâneas/terapia , Úmero/patologia , Ablação por Radiofrequência/métodos , Adenocarcinoma/secundário , Adenocarcinoma/terapia , Idoso , Cimentos Ósseos , Neoplasias Ósseas/secundário , Neoplasias Ósseas/terapia , Fios Ortopédicos , Dor do Câncer/diagnóstico , Dor do Câncer/etiologia , Dor do Câncer/psicologia , Fixação Interna de Fraturas/instrumentação , Fixação Interna de Fraturas/métodos , Fraturas Espontâneas/etiologia , Fraturas Espontâneas/patologia , Humanos , Úmero/cirurgia , Masculino , Micro-Ondas/uso terapêutico , Medição da Dor , Qualidade de Vida , Neoplasias Gástricas/patologia , Neoplasias Gástricas/terapia , Resultado do Tratamento
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