Structure and function of the digestive system in molluscs.

The phylum Mollusca is one of the largest and more diversified among metazoan phyla, comprising many thousand species living in ocean, freshwater and terrestrial ecosystems. Mollusc-feeding biology is highly diverse, including omnivorous grazers, herbivores, carnivorous scavengers and predators, and even some parasitic species. Consequently, their digestive system presents many adaptive variations. The digestive tract starting in the mouth consists of the buccal cavity, oesophagus, stomach and intestine ending in the anus. Several types of glands are associated, namely, oral and salivary glands, oesophageal glands, digestive gland and, in some cases, anal glands. The digestive gland is the largest and more important for digestion and nutrient absorption. The digestive system of each of the eight extant molluscan classes is reviewed, highlighting the most recent data available on histological, ultrastructural and functional aspects of tissues and cells involved in nutrient absorption, intracellular and extracellular digestion, with emphasis on glandular tissues.

Glass reinforced plastic (GRP) boats and the impact on coastal environment - Evidence of fibreglass ingestion by marine bivalves from natural populations.

Classified as marine debris, man made materials are polluting the world's oceans. Recently, glass reinforced plastic (GRP) has been shown to degrade and contaminate the coasts. In this pioneering study, fibreglass particles have been detected in the soft parts of oysters and mussels collected from natural populations, in front of an active boatyard. The presence of particulate glass, with concentrations up to 11,220 particles/kg ww in Ostrea edulis and 2740 particles/kg ww in Mytilus edulis, was confirmed by micro Raman spectroscopy. The results showed higher accumulation during the winter months, when boat maintenance activities are peaking and, through repair work, the release of glass fibres in the environment is more likely. Bivalves are considered high risk species due to their sessile nature and extensive filter feeding behaviour. The microparticle inclusion may contribute to adverse impacts on physiological processes and eventually to a decline in the overall health and subsequent death of the animal. The high costs involved in the proper GRP disposal and the lack of recycling facilities worldwide lead to boat abandonement and further contamination of the coasts. For the first time this study presents the extensive fibreglass contamination of natural bivalve populations, in a popular South England sailing harbour, designated a biological and geological site of specific scientific interest (SSRI).

The odd couple: Caffeine and microplastics. Morphological and physiological changes in Mytilus galloprovincialis.

In recent years, the presence of pharmaceuticals and microplastics (MPs) in aquatic ecosystems has raised concerns about their environmental impact. This study explores the combined effects of caffeine, a common pharmaceutical pollutant, and MPs on the marine mussel Mytilus galloprovincialis. Caffeine, at concentrations of 20.0 µg L-1, and MPs (1 mg L-1, 35-50 µm size range), was used to mimic real-world exposure scenarios. Two hundred M. galloprovincialis specimens were divided into four groups: caffeine, MPs, Mix (caffeine + MPs), and Control. After a two-week acclimation period, the mollusks were subjected to these pollutants in oxygen-aerated aquariums under controlled conditions for 14 days. Histopathological assessments were performed to evaluate gill morphology. Cellular volume regulation and digestive gland cell viability were also analyzed. Exposure to caffeine and MPs induced significant morphological changes in M. galloprovincialis gills, including cilia loss, ciliary disk damage, and cellular alterations. The chitinous rod supporting filaments also suffered damage, potentially due to MP interactions, leading to hemocyte infiltration and filament integrity compromise. Hemocytic aggregation suggested an inflammatory response to caffeine. In addition, viability assessments of digestive gland cells revealed potential damage to cell membranes and function, with impaired cell volume regulation, particularly in the Mix group, raising concerns about nutrient metabolism disruption and organ function compromise. These findings underscore the vulnerability of M. galloprovincialis to environmental pollutants and emphasize the need for monitoring and mitigation efforts. RESEARCH HIGHLIGHTS: The synergy of caffeine and microplastics (MPs) in aquatic ecosystems warrants investigation. MPs and caffeine could affect gill morphology of Mytilus galloprovincialis. Caffeine-exposed cells had lower viability than the control group in the NR retention test. MPs and mix-exposed cells struggled to recover their volume.

Exploring the co-exposure effects of environmentally relevant microplastics and an estrogenic mixture on the metabolome of the Sydney rock oyster.

In aquatic environments the concurrent exposure of molluscs to microplastics (MPs) and estrogens is common, as these pollutants are frequently released by wastewater treatment plants into estuaries. Therefore, this study aimed to evaluate the independent and co-exposure impacts of polyethylene microplastics (PE-MPs) and estrogenic endocrine-disrupting chemicals (EEDCs) at environmentally relevant concentrations on polar metabolites and morphological parameters of the Sydney rock oyster. A seven-day acute exposure revealed no discernible differences in morphology; however, significant variations in polar metabolites were observed across oyster tissues. The altered metabolites were mostly amino acids, carbohydrates and intermediates of the Kreb's cycle. The perturbation of metabolites were tissue and sex-specific. All treatments generally showed an increase of metabolites relative to controls - a possible stimulatory and/or a potential hormetic response. The presence of MPs impeded the exposure of adsorbed and free EEDCs potentially due to the selective feeding behaviour of oysters to microplastics, favouring algae over similar-sized PE-MPs, and the formation of an eco/bio-corona involving faeces, pseudo-faeces, natural organic matter, and algae.

Proteomic analysis of digestive tract peptidases and lipases from the invasive gastropod Pomacea canaliculata.

BACKGROUND: The invasive gastropod Pomacea canaliculata has received great attention in the last decades as a result of its negative impact on crops agriculture, yet knowledge of their digestive physiology remains incomplete, particularly the enzymatic breakdown of macromolecules such as proteins and lipids. RESULTS: Discovery proteomics revealed aspartic peptidases, cysteine peptidases, serine peptidases, metallopeptidases and threonine peptidases, as well as acid and neutral lipases and phospholipases along the digestive tract of P. canaliculata. Peptides specific to peptidases (139) and lipases (14) were quantified by targeted mass spectrometry. Digestion begins in the mouth via diverse salivary peptidases (nine serine peptidases; seven cysteine peptidases, one aspartic peptidase and 22 metallopeptidases) and then continues in the oesophagus (crop) via three luminal metallopeptidases (Family M12) and six serine peptidases (Family S1). Downstream, the digestive gland provides a battery of enzymes composed of aspartic peptidase (one), cysteine peptidases (nine), serine peptidases (12) and metallopeptidases (24), including aminopeptidases, carboxypeptidases and dipeptidases). The coiled gut has M1 metallopeptidases that complete the digestion of small peptides. Lipid extracellular digestion is completed by triglyceride lipases. CONCLUSION: From an integrative physiological and anatomical perspective, P. canaliculata shows an unexpected abundance and diversity of peptidases, which participate mainly in extracellular digestion. Moreover, the previously unknown occurrence of luminal lipases from the digestive gland is reported for the first time. Salivary and digestive glands were the main tissues involved in the synthesis and secretion of these enzymes, but plausibly the few luminally exclusive peptidases are secreted by ventrolateral pouches or epithelial unicellular glands. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Microplastic contamination in commercially important bivalves from the southwest coast of India.

Due to the ever-increasing production of plastic litter and its subsequent accumulation as microplastic in the environment, the pollution caused by microplastics is considered as a global menace, especially in the coastal ecosystem. Occurrence of microplastics in water and three commercially important bivalves, Viz. green mussel (Perna viridis), edible oyster (Magallana bilineata) and black clam (Villorita cyprinoides) from five different locations of southwest coast of India was studied. The highest abundance of microplastics was observed in water samples from Periyar River (163.67 items L-1). Among bivalves, the highest abundance of microplastics was observed in clams from Periyar River (digestive gland: 22.8 g-1; gill: 29.6 g-1), whereas the lowest abundance was observed in mussels sampled from Vembanad estuary (digestive gland: 5.6 g-1; gill: 8.5 g -1). Fibers were the most prevalent type of microplastics found in bivalve tissues across each location. Microplastics less than 2 mm were the most prevalent based on size. Polypropylene and high-density polyethylene were the two types of microplastics observed based on the results of Raman spectroscopy. No relationship was observed between shell length, tissue weight and microplastic abundance. A strong positive correlation was observed between the microplastic presence in water and bivalve tissues. The usefulness of sedentary bivalves in assessing the aquatic pollution has been validated through this study.

Dynamic of small polyethylene microplastics (≤10 µm) in mussel's tissues.

MPs' uptake and tissue accumulation were investigated in mussel exposed to a single dose (2.85 mg ind-1, 3 mg l-1) of a heterogeneous mixture of irregularly shaped particles of HDPE (mainly ≤10 µm), followed by a 7 days depuration period. The results showed that mussels efficiently cleared MPs from water during exposure, and that MPs were accumulated in digestive gland and gills during depuration. In digestive gland, the amount and size of the MPs accumulated decreased with time, indicating a slower processing and elimination of small MPs than of larger ones. In gills, MPs' burdens increased with time, the MPs accumulated were the smallest ones, suggesting the translocation of small MPs from the digestive system to the gills. The hazardous potential of the smaller fraction of MPs (≤4 µm) underlined that more focus should be directed towards the accumulation and effects of this fraction of MPs in the marine environment.

Mussel digestive gland as a model tissue for assessing xenobiotics: An overview.

Control strategies and routine biomonitoring programs are commonly performed worldwide using sentinel marine invertebrates, such as mussels of the genus Mytilus, for assessing the "health status" of the aquatic environment. Those species can accumulate and tolerate xenobiotics at levels higher than those being present into the aquatic environment, thus providing accurate and reliable biological endpoints (e.g. physiological, behavioral, cellular, biochemical and molecular indices) that can be measured in their tissues. Taking under consideration the significance of bivalves for assessing the environmental hazard of xenobiotics being present into the water medium, as well as the key role of digestive gland as a target-tissue for the compounds ingested in the organism, the present study aimed to summarize available data on the effects of different categories of xenobiotic compounds, previously characterized as a potential threat for the marine ecosystems. In this context, different types of pharmaceuticals and personal care products (PPCPs), biocides, microplastics (MPs) and nanoparticles (NPs), currently investigated in mussels' digestive gland, using a battery of experimental approaches and analytical methods, as well as stress indices evaluation, are briefly described and further discussed in order to elucidate not only the presence and the toxic mode of action of xenobiotics, but also the important role of the digestive gland as a reliable target-tissue for investigating the effects of xenobiotics at cellular, biochemical, and molecular levels.