RESUMO
In recent years, nanozymes based on two-dimensional (2D) nanomaterials have been receiving great interest for cancer photothermal therapy. 2D materials decorated with nanoparticles (NPs) on their surface are advantageous over conventional NPs and 2D material based systems because of their ability to synergistically improve the unique properties of both NPs and 2D materials. In this work, we report a nanozyme based on flower-like MoS2nanoflakes (NFs) by decorating their flower petals with NCeO2using polyethylenimine (PEI) as a linker molecule. A detailed investigation on toxicity, biocompatibility and degradation behavior of fabricated nanozymes in wild-typeDrosophila melanogastermodel revealed that there were no significant effects on the larval size, morphology, larval length, breadth and no time delay in changing larvae to the third instar stage at 7-10 d for MoS2NFs before and after NCeO2decoration. The muscle contraction and locomotion behavior of third instar larvae exhibited high distance coverage for NCeO2decorated MoS2NFs when compared to bare MoS2NFs and control groups. Notably, the MoS2and NCeO2-PEI-MoS2NFs treated groups at 100µg ml-1covered a distance of 38.2 mm (19.4% increase when compared with control) and 49.88 mm (no change when compared with control), respectively. High-resolution transmission electron microscopy investigations on the new born fly gut showed that the NCeO2decoration improved the degradation rate of MoS2NFs. Hence, nanozymes reported here have huge potential in various fields ranging from biosensing, cancer therapy and theranostics to tissue engineering and the treatment of Alzheimer's disease and retinal therapy.
Assuntos
Materiais Biocompatíveis/toxicidade , Cério/toxicidade , Dissulfetos/toxicidade , Molibdênio/toxicidade , Nanoestruturas/toxicidade , Animais , Materiais Biocompatíveis/administração & dosagem , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacocinética , Cério/administração & dosagem , Cério/química , Cério/farmacocinética , Dissulfetos/administração & dosagem , Dissulfetos/química , Dissulfetos/farmacocinética , Drosophila melanogaster , Trato Gastrointestinal/metabolismo , Larva/efeitos dos fármacos , Larva/crescimento & desenvolvimento , Larva/metabolismo , Locomoção/efeitos dos fármacos , Teste de Materiais , Taxa de Depuração Metabólica , Molibdênio/administração & dosagem , Molibdênio/química , Molibdênio/farmacocinética , Contração Muscular/efeitos dos fármacos , Nanoestruturas/administração & dosagem , Nanoestruturas/química , Polietilenoimina/administração & dosagem , Polietilenoimina/química , Polietilenoimina/farmacocinética , Polietilenoimina/toxicidade , Espécies Reativas de Oxigênio/metabolismoRESUMO
Cerium oxide (CeO2 ) nanoparticles have unique redox properties and exert excellent antioxidant effects in the biological environment. In recent years, many researchers have focused on the CeO2 nanoparticles as an effective antioxidant drug in the prevention and treatment of various diseases. However, the toxicity of CeO2 nanoparticles in vivo remains controversial and still needs intensive research. Therefore, the objective of this study is to investigate the pulmonary and systemic toxicity in rats after 14 days of exposure to the PEGylated CeO2 nanoparticles (abbreviated as CNPs; exposure dose of 2, 10, or 20 mg/kg) through a single intratracheal instillation (IT). We assessed the indicators of lung injury and the pathological damage degree of lung tissue. The bronchoalveolar lavage fluid (BALF) analysis and lung histopathology revealed the occurrence of slight pulmonary inflammation in the 20-mg/kg experimental group rats. However, the inflammation factors in the lung tissue of every group rats did not significantly increase, and the levels of superoxide dismutase (SOD) and glutathione (GSH) in lung tissue homogenate rose considerably in the experimental groups. Collectively, these results indicated that pulmonary exposure by the high dose of CNPs could induce mild pulmonary inflammation but did not cause severe systemic toxicity. Moreover, we speculate that the mechanism of pulmonary toxicity of CNPs in rats was due to the autophagic death of healthy lung epithelial cells mediated by endoplasmic reticulum stress. Our results implicate that CNPs can be safely used as an antioxidant drug for the oxidative stress pulmonary diseases.
Assuntos
Antioxidantes/toxicidade , Cério/toxicidade , Nanopartículas Metálicas/toxicidade , Polietilenoglicóis/toxicidade , Animais , Antioxidantes/farmacologia , Líquido da Lavagem Broncoalveolar , Inflamação/patologia , Pulmão/efeitos dos fármacos , Pneumopatias/patologia , Masculino , Nanopartículas/toxicidade , Estresse Oxidativo/efeitos dos fármacos , Preparações Farmacêuticas , Pneumonia/patologia , Polietilenoglicóis/farmacologia , RatosRESUMO
BACKGROUND: A standard short-term inhalation study (STIS) was applied for hazard assessment of 13 metal oxide nanomaterials and micron-scale zinc oxide. METHODS: Rats were exposed to test material aerosols (ranging from 0.5 to 50 mg/m3) for five consecutive days with 14- or 21-day post-exposure observation. Bronchoalveolar lavage fluid (BALF) and histopathological sections of the entire respiratory tract were examined. Pulmonary deposition and clearance and test material translocation into extra-pulmonary organs were assessed. RESULTS: Inhaled nanomaterials were found in the lung, in alveolar macrophages, and in the draining lymph nodes. Polyacrylate-coated silica was also found in the spleen, and both zinc oxides elicited olfactory epithelium necrosis. None of the other nanomaterials was recorded in extra-pulmonary organs. Eight nanomaterials did not elicit pulmonary effects, and their no observed adverse effect concentrations (NOAECs) were at least 10 mg/m3. Five materials (coated nano-TiO2, both ZnO, both CeO2) evoked concentration-dependent transient pulmonary inflammation. Most effects were at least partially reversible during the post-exposure period.Based on the NOAECs that were derived from quantitative parameters, with BALF polymorphonuclear (PMN) neutrophil counts and total protein concentration being most sensitive, or from the severity of histopathological findings, the materials were ranked by increasing toxic potency into 3 grades: lower toxic potency: BaSO4; SiO2.acrylate (by local NOAEC); SiO2.PEG; SiO2.phosphate; SiO2.amino; nano-ZrO2; ZrO2.TODA; ZrO2.acrylate; medium toxic potency: SiO2.naked; higher toxic potency: coated nano-TiO2; nano-CeO2; Al-doped nano-CeO2; micron-scale ZnO; coated nano-ZnO (and SiO2.acrylate by systemic no observed effect concentration (NOEC)). CONCLUSION: The STIS revealed the type of effects of 13 nanomaterials, and micron-scale ZnO, information on their toxic potency, and the location and reversibility of effects. Assessment of lung burden and material translocation provided preliminary biokinetic information. Based upon the study results, the STIS protocol was re-assessed and preliminary suggestions regarding the grouping of nanomaterials for safety assessment were spelled out.
Assuntos
Nanoestruturas/toxicidade , Administração por Inalação , Aerossóis , Animais , Apoptose/efeitos dos fármacos , Sulfato de Bário/toxicidade , Carga Corporal (Radioterapia) , Líquido da Lavagem Broncoalveolar , Proliferação de Células/efeitos dos fármacos , Cério/toxicidade , Materiais Revestidos Biocompatíveis , Pulmão/patologia , Masculino , Microscopia Eletrônica de Varredura , Nanoestruturas/administração & dosagem , Óxidos/toxicidade , Ratos , Ratos Wistar , Dióxido de Silício/toxicidade , Titânio/toxicidade , Aumento de Peso/efeitos dos fármacos , Óxido de Zinco/toxicidade , Zircônio/toxicidadeRESUMO
Coated nanoparticles (NPs) will end up in the environment due to their proposed use in agricultural applications and may potentially cause toxic effects due to their unique properties. To determine the effects of coated NPs on zebrafish (Danio rerio) development, we tested aqueous poly(acrylic acid) (PAA)-coated metal-oxide NPs including TiO2, ZnO, Fe2O3, and CeO2, as well as the polymer coating alone (nanocapsule). Zebrafish embryos were exposed to NPs over a 72 h period at 1, 10, 50, 100, 200, 400, 800, 1200, 1600, and 2000 mg/L to measure various end points. We also ran free metal controls. Time-dependent changes in physicochemical properties of NPs were characterized using dynamic light scattering. Dissolution experiments over 72 h showed minimal free metals were present in stock suspensions and released from the NPs. Interestingly, nanocapsules (≥ 800 mg/L) cause inhibition of hatch, and we suggest that a low pH environment may explain this effect. This study has also demonstrated that CeO2 NPs and nanocapsules containing Nile red are able to traverse the chorion. Overall, our findings indicate that each NP type is stable and neither the NP or encapsulating PAA coating causes apparent toxicity to developing zebrafish.
Assuntos
Cério/toxicidade , Compostos Férricos/toxicidade , Nanopartículas/química , Polímeros/toxicidade , Titânio/toxicidade , Óxido de Zinco/toxicidade , Animais , Cério/química , Compostos Férricos/química , Polímeros/química , Titânio/química , Peixe-Zebra , Óxido de Zinco/químicaRESUMO
Oxidative stress, which is one of the main harmful mechanisms of pathologies including ischemic stroke, contributes to both neurons and endothelial cell damages, leading to vascular lesions. Although many antioxidants are tested in preclinical studies, no treatment is currently available for stroke patients. Since cerium oxide nanoparticles (CNPs) exhibit remarkable antioxidant capacities, the objective is to develop an innovative coating to enhance CNPs biocompatibility without disrupting their antioxidant capacities or enhance their toxicity. This study reports the synthesis and characterization of functional polymers and their impact on the enzyme-like catalytic activity of CNPs. To study the toxicity and the antioxidant properties of CNPs for stroke and particularly endothelial damages, in vitro studies are conducted on a cerebral endothelial cell line (bEnd.3). Despite their internalization in bEnd.3 cells, coated CNPs are devoid of cytotoxicity. Microscopy studies report an intracellular localization of CNPs, more precisely in endosomes. All CNPs reduces glutamate-induced intracellular production of reactive oxygen species (ROS) in endothelial cells but one CNP significantly reduces both the production of mitochondrial superoxide anion and DNA oxidation. In vivo studies report a lack of toxicity in mice. This study therefore describes and identifies biocompatible CNPs with interesting antioxidant properties for ischemic stroke and related pathologies.
Assuntos
Cério , Nanopartículas , Animais , Antioxidantes/farmacologia , Cério/toxicidade , Células Endoteliais , Humanos , Camundongos , PolímerosRESUMO
Studying the influence of nanomaterials on the microstructure and mechanical properties of cells is essential to guide the biological applications of nanomaterials. In this article, the effects of the first synthesized PDA@CeO2 nanoparticles (NPs) with multiple ROS scavenging activities on cell ultra-morphology and mechanical properties were investigated by atomic force microscopy (AFM). After the cells were exposed to PDA@CeO2 NPs, there was no obvious change in cell morphology, but the Young's modulus of the cells was increased. On the contrary, after the cells were damaged by H2O2, the secreted molecules appeared on the cell surface, and the Young's modulus was decreased significantly. However, PDA@CeO2 NPs could effectively inhibit the reduction of the Young's modulus caused by oxidative stress damage. PDA@CeO2 NPs could also protect F-actin from oxidative stress damage and maintain the stability of the cytoskeleton. This work investigates the intracellular antioxidant mechanism of nanomaterials from the changes in the microstructure and biomechanics of living cells, providing a new analytical approach to explore the biological effects of nanomaterials.
Assuntos
Cério/farmacologia , Sequestradores de Radicais Livres/farmacologia , Indóis/farmacologia , Nanopartículas/química , Polímeros/farmacologia , Células 3T3-L1 , Actinas/metabolismo , Animais , Cério/química , Cério/toxicidade , Módulo de Elasticidade , Sequestradores de Radicais Livres/química , Indóis/química , Indóis/toxicidade , Camundongos , Nanopartículas/toxicidade , Polímeros/química , Polímeros/toxicidadeRESUMO
The increased applications of nanoparticles in a wide range of industrial fields raise the concern about their potential toxicity to human. The aim of this study was to assess and compare the toxicity of four different oxide nanoparticles (Al2O3, CeO2, TiO2 and ZnO) to human lung epithelial cells, A549 carcinoma cells and L-132 normal cells, in vitro. We focused on the toxicological effects of the present nanoparticles on cell proliferation, cell viability, membrane integrity and oxidative stress. The long-term cytotoxicity of nanoparticles was also evaluated by employing the clonogenic assay. Among four nanoparticles tested, ZnO exhibited the highest cytotoxicity in terms of cell proliferation, cell viability, membrane integrity and colony formation in both cell lines. Al2O3, CeO2 and TiO2 showed little adverse effects on cell proliferation and cell viability. However, TiO2 induced oxidative stress in a concentration- and time-dependent manner. CeO2 caused membrane damage and inhibited colony formation in long-term, but with different degree depending on cell lines. Al2O3 seems to be less toxic than the other nanoparticles even after long time exposure. These results highlight the need for caution during manufacturing process of nanomaterials as well as further investigation on the toxicity mechanism.
Assuntos
Óxido de Alumínio/toxicidade , Cério/toxicidade , Células Epiteliais/efeitos dos fármacos , Células Epiteliais/fisiologia , Nanopartículas/toxicidade , Titânio/toxicidade , Óxido de Zinco/toxicidade , Apoptose/efeitos dos fármacos , Materiais Biocompatíveis/toxicidade , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Relação Dose-Resposta a Droga , Células Epiteliais/citologia , Humanos , Teste de MateriaisRESUMO
Nanoceria has a broad variety of industrial and pharmacological applications due to its antioxidant activity. Nanoceria can be modified by surface coating with polyelectrolyte brushes. Brushes can increase the surface charge of nanoceria, providing greater aqueous stability while reducing agglomeration. However, surface-coating also behaves as a barrier around nanoceria, affecting its redox equilibrium and, hence, its biological and toxicological properties. In the present study, we examined whether bare nanoceria (CeO2; 80-150 nm) and nanoceria modified by surface polymer brush, using negatively charged polyacrylic acid (CeO2@PAA) and positively charged poly (2-(methacryloyloxy)ethyl-trimethyl-ammonium chloride (CeO2@PMETAC), could induce systemic toxicity. As CeO2 has limited colloidal stability, which might result in vascular occlusion, intraperitoneal injection was used instead of intravenous administration. C57Bl/6 mice were four times injected with three different doses of each nanoceria-based sample (corresponding to 1.8, 5.3 and 16 mg Ce/kg BW/administration) for a total period of 14 days. CeO2@PMETAC induced a significant dose-dependent neutrophilia. Histopathological evaluation showed inflammatory processes in the capsule of liver, kidney, and spleen of animals at all doses of CeO2@PMETAC, and with the highest dose of CeO2@PAA and CeO2. However, none of the nanoceria-based samples tested increased the level of DNA damage or micronuclei in blood cells, even though Ce was detected by inductively coupled plasma mass spectrometry analyses in the bone marrow. Only CeO2@PMETAC induced the presence of megakaryocytes in the spleen. A higher accumulation of Ce in mononuclear phagocyte system organs (liver, spleen and bone marrow) was observed after CeO2@PMETAC treatment compared with CeO2@PAA and CeO2.
Assuntos
Resinas Acrílicas/química , Cério/toxicidade , Nanopartículas/toxicidade , Ácidos Polimetacrílicos/química , Compostos de Amônio Quaternário/química , Vísceras/efeitos dos fármacos , Animais , Cério/química , Dano ao DNA , Relação Dose-Resposta a Droga , Feminino , Injeções Intraperitoneais , Camundongos , Camundongos Endogâmicos C57BL , Nanopartículas/química , Especificidade de Órgãos , Tamanho da Partícula , Propriedades de Superfície , Termogravimetria , Vísceras/patologiaRESUMO
The presence of abundant extracellular polymeric substances (EPS) play a vital role in affecting heteroaggregation process and toxicity of nanoparticles (NPs) to Microcystis aeruginosa. Interactions between n-CeO2 and cyanobacteria with/without EPS and the toxicity of n-CeO2 to M. aeruginosa were investigated in this study. Aggregation kinetics of n-CeO2 under both soluble EPS (SEPS) and bound EPS (BEPS) indicated the presence of EPS could induced the formation of EPS-NPs aggregates. Heteroaggregation between cells and n-CeO2 was confirmed through co-settling experiment and SEM-EDS observation. SEPS contributed to the observable heteroaggregation using spectral measurement. Heteroaggregation between cells and n-CeO2 under no BEPS was hardly obtained through spectral measurement, but SEM-EDS observation convinced this process. And the DLVO theory explained this heteroaggregation process under various EPS conditions, where the energy barrier decreased with gradual EPS extraction. In addition, the order for 96â¯h half growth inhibition concentration (IC50) was Raw M9 > M9-SEPS > M9+BEPS > M9-BEPS. These results revealed that not all heteroaggregation between cell-NPs can lead to the NPs toxicity to cells. BEPS act more important role in buffering against the toxicity of NPs from ambient adverse factors, but SEPS increase the stability of NPs which could aggravate the adverse effects of NPs in the environment.
Assuntos
Cério/toxicidade , Microcystis/efeitos dos fármacos , Nanopartículas/toxicidade , Polissacarídeos Bacterianos/análise , Cério/química , Concentração de Íons de Hidrogênio , Microcystis/metabolismo , Nanopartículas/química , Polímeros/metabolismoRESUMO
Effects of functionalization and grafting of TiO2, CeO2, and SiO2 nanoparticles (NPs) were investigated, and toxicity of pristine, functionalized, and grafted NP towards Daphnia magna was measured. Surface functionalization of NP with amine groups decreased hydrophobicity of NP. When NPs were hydrophilic, they were less toxic than hydrophobic NP towards D. magna. Grafting agents influenced toxicity: no toxicity of NP was observed when bio-based and hydrogenated synthetic polymers were used, whereas perfluorinated polymers induced a higher toxicity.
Assuntos
Cério/toxicidade , Daphnia/efeitos dos fármacos , Nanopartículas/toxicidade , Polímeros/toxicidade , Dióxido de Silício/toxicidade , Titânio/toxicidade , Animais , Bioensaio , Cério/química , Daphnia/crescimento & desenvolvimento , Nanopartículas/química , Polímeros/síntese química , Dióxido de Silício/química , Titânio/química , Poluentes Químicos da Água/químicaRESUMO
The use of different biomaterials with the ability to accelerate the repair and regeneration processes is of great importance in tissue engineering strategies. On this point, cerium oxide nanoparticles (CNPs or nanoceria) have recently attracted much attention due to their excellent biological properties including anti-oxidant, anti-inflammation and antibacterial activities as well as high angiogenic potential. The results of incorporation of these nano-sized particles into various constructs and scaffolds designed for tissue engineering applications have proven the success of this strategy in terms of improving healing process of different tissues. In this review, we first summarize the physicochemical and biological properties of nanoceria in brief and then present its usability in tissue engineering strategies based on the currently available published reports.
Assuntos
Materiais Biocompatíveis/química , Cério/química , Nanopartículas Metálicas/química , Indutores da Angiogênese/química , Antibacterianos/química , Anti-Inflamatórios/química , Antioxidantes/química , Cério/toxicidade , Sistemas de Liberação de Medicamentos/métodos , Humanos , Tamanho da Partícula , Regeneração , Propriedades de Superfície , Nanomedicina Teranóstica/métodos , Engenharia Tecidual/métodosRESUMO
CeO2 nanoparticles (NPs) have shown promising approaches as therapeutic agents in biology and medical sciences. The physicochemical properties of CeO2-NPs, such as size, agglomeration status in liquid, and surface charge, play important roles in the ultimate interactions of the NP with target cells. Recently, CeO2-NPs have been synthesized through several bio-directed methods applying natural and organic matrices as stabilizing agents in order to prepare biocompatible CeO2-NPs, thereby solving the challenges regarding safety, and providing the appropriate situation for their effective use in biomedicine. This review discusses the different green strategies for CeO2-NPs synthesis, their advantages and challenges that are to be overcome. In addition, this review focuses on recent progress in the potential application of CeO2-NPs in biological and medical fields. Exploiting biocompatible CeO2-NPs may improve outcomes profoundly with the promise of effective neurodegenerative therapy and multiple applications in nanobiotechnology.
Assuntos
Cério/química , Química Verde/métodos , Nanopartículas/química , Biopolímeros/química , Técnicas Biossensoriais , Cério/toxicidade , Enzimas/metabolismo , Nanopartículas/toxicidadeRESUMO
This study examined the impact of surface functionalization and charge on ceria nanomaterial toxicity to Caenorhabditis elegans. The examined endpoints included mortality, reproduction, protein expression, and protein oxidation profiles. Caenorhabditis elegans were exposed to identical 2-5nm ceria nanomaterial cores which were coated with cationic (diethylaminoethyl dextran; DEAE), anionic (carboxymethyl dextran; CM), and non-ionic (dextran; DEX) polymers. Mortality and reproductive toxicity of DEAE-CeO2 was approximately two orders of magnitude higher than for CM-CeO2 or DEX-CeO2. Two-dimensional gel electrophoresis with orbitrap mass spectrometry identification revealed changes in the expression profiles of several mitochondrial-related proteins and proteins that are expressed in the C. elegans intestine. However, each type of CeO2 material exhibited a distinct protein expression profile. Increases in protein carbonyls and protein-bound 3-nitrotyrosine were also observed for some proteins, indicating oxidative and nitrosative damage. Taken together the results indicate that the magnitude of toxicity and toxicity pathways vary greatly due to surface functionalization of CeO2 nanomaterials.
Assuntos
Cério/toxicidade , Nanoestruturas/toxicidade , Polímeros/química , Animais , Caenorhabditis elegans , Proteínas de Caenorhabditis elegans/genética , Proteínas de Caenorhabditis elegans/metabolismo , Cério/química , Regulação da Expressão Gênica/efeitos dos fármacos , Mucosa Intestinal/metabolismo , Intestinos/efeitos dos fármacos , Nanopartículas Metálicas/toxicidade , Nanoestruturas/química , Propriedades de SuperfícieRESUMO
Extracellular polymeric substances (EPS) are key components of the cyanobacterium Microcystis aeruginosa and play an important role in cyanobacteria blooms formation. Here, we analyzed the effects of 48-h exposure to nanosized CeO2 (n-CeO2), CuO (n-CuO), and ZnO (n-ZnO) on the production and composition of EPS of M. aeruginosa. Toxicity experiments revealed that soluble nanoparticles (NPs) (n-ZnO, n-CuO) demonstrated higher toxicity to cells and caused membrane damage. The production of LB-EPS increased by 34.48, 20.09, and 46.33 %, and TB-EPS increased by -5.78, 22.3, and -2.67 % in the presence of n-CeO2, n-CuO, and n-ZnO NPs, respectively, and polysaccharides are the main incremental portion compared with protein and humic acids. Three-dimensional excitation-emission fluorescence spectra revealed the enhancement of fulvic-humic-like and disappearance of tyrosine aromatic substances in TB-EPS compared with the slight changes observed in LB-EPS. Fourier-transform infrared spectroscopy illustrated the susceptibility of -NH2 and double-bonded carbon and oxygen in amides to three types of NPs. These results improve our understanding of the potential influence of NPs on the aggregation behaviors of cyanobacteria and formation process of cyanobacteria blooms. Graphical abstract á .
Assuntos
Microcystis/efeitos dos fármacos , Nanopartículas/toxicidade , Polímeros/metabolismo , Polissacarídeos Bacterianos/metabolismo , Poluentes Químicos da Água/toxicidade , Carbono/metabolismo , Cério/química , Cério/toxicidade , Cobre/química , Cobre/toxicidade , Eutrofização , Substâncias Húmicas/análise , Microcystis/metabolismo , Nanopartículas/química , Polímeros/química , Polissacarídeos Bacterianos/química , Proteínas/metabolismo , Espectroscopia de Infravermelho com Transformada de Fourier , Poluentes Químicos da Água/química , Óxido de Zinco/química , Óxido de Zinco/toxicidadeRESUMO
Despite many studies, the impact of ceramic particles on cell behavior remains unclear. The aim of the present study was to investigate the effects of nano-sized ceramic particles on fibroblastic cells. Fibroblasts (dermal fibroblasts freshly isolated from skin samples and WI26 fibroblastic cells) were cultured in a monolayer in the presence of alumina or cerium-zirconia particles (≈50 nm diameter) at two concentrations (100 or 500 µg ml-1). Fluorescent alumina particles were also used. The following properties were analyzed: cell morphology, cytoplasmic ceramic incorporation (using confocal and transmission electron microscopy) and migration (using a silicon insert). Sedimentation field-flow fractionation (SdFFF) was also used to evaluate the rate of incorporation of ceramic particles into the cells. Finally, after treatment with various concentrations of ceramic particles, fibroblasts were also included in a collagen type I lattice constituting a dermal equivalent (DE), and the collagen lattice retraction and cell proliferation were evaluated. In monolayer conditions, the presence of both alumina and cerium-zirconia ceramic particles did not cause any deleterious effects on cultured cells (dermal fibroblast and WI26 cells) and cell fate was not affected in any way by the presence of ceramic particles in the cytoplasm. Confocal (using fluorescent alumina particles) and electron microscopy (using both alumina and cerium-zirconia particles) showed that ceramic particles were internalized in the WI26 cells. Using fluorescent membrane labeling and fluorescent alumina particles, a membrane was observed around the particle-containing vesicles present in the cytoplasm. Electron microscopy on WI26 cells showed the presence of a classical bilayer membrane around the ceramic particles. Interestingly, SdFFF confirmed that some dermal fibroblasts contained many alumina ceramic particles while others contained very few; in WI26 cells, the uptake of alumina ceramic was more homogeneous. In DE, collagen lattice retraction and cell proliferation were unchanged when WI26 fibroblastic cells contained alumina or cerium-zirconia ceramic particles. Our data suggest that ceramic particles are internalized in the cells by endocytosis. The presence of ceramic particles in the cytoplasm has no affect on cell behavior, confirming the excellent biocompatibility of this material and anticipating a minimal harmful effect of potential wear debris.
Assuntos
Materiais Biocompatíveis/toxicidade , Cerâmica/toxicidade , Fibroblastos/efeitos dos fármacos , Nanopartículas/toxicidade , Óxido de Alumínio/química , Óxido de Alumínio/toxicidade , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacocinética , Linhagem Celular , Movimento Celular/efeitos dos fármacos , Células Cultivadas , Cerâmica/química , Cerâmica/farmacocinética , Cério/química , Cério/toxicidade , Fibroblastos/citologia , Fibroblastos/fisiologia , Humanos , Teste de Materiais , Microscopia Confocal , Microscopia Eletrônica de Transmissão , Nanopartículas/química , Zircônio/química , Zircônio/toxicidadeRESUMO
An aqueous dispersion of poly (acrylic acid)-stabilised cerium oxide (CeO2) nanoparticles (PAA-CeO2) was evaluated for its stability in a range of freshwater ecotoxicity media (MHRW, TG 201 and M7), with and without natural organic matter (NOM). In a 15 day dispersion stability study, PAA-CeO2 did not undergo significant aggregation in any media type. Zeta potential varied between media types and was influenced by PAA-CeO2 concentration, but remained constant over 15 days. NOM had no influence on PAA-CeO2 aggregation or zeta potential. The ecotoxicity of the PAA-CeO2 dispersion was investigated in 72 h algal growth inhibition tests using the freshwater microalgae Pseudokirchneriella subcapitata. PAA-CeO2 EC50 values for growth inhibition (GI; 0.024 mg/L) were 2-3 orders of magnitude lower than pristine CeO2 EC50 values reported in the literature. The concentration of dissolved cerium (Ce(3+)/Ce(4+)) in PAA-CeO2 exposure suspensions was very low, ranging between 0.5 and 5.6 µg/L. Free PAA concentration in the exposure solutions (0.0096-0.0384 mg/L) was significantly lower than the EC10 growth inhibition (47.7 mg/L) value of pure PAA, indicating that free PAA did not contribute to the observed toxicity. Elemental analysis indicated that up to 38% of the total Cerium becomes directly associated with the algal cells during the 72 h exposure. TOF-SIMS analysis of algal cell wall compounds indicated three different modes of action, including a significant oxidative stress response to PAA-CeO2 exposure. In contrast to pristine CeO2 nanoparticles, which rapidly aggregate in standard ecotoxicity media, PAA-stabilised CeO2 nanoparticles remain dispersed and available to water column species. Interaction of PAA with cell wall components, which could be responsible for the observed biomarker alterations, could not be excluded. This study indicates that the increased dispersion stability of PAA-CeO2 leads to an increase in toxicity compared to pristine non-stabilised forms.
Assuntos
Resinas Acrílicas/química , Cério/toxicidade , Clorófitas/efeitos dos fármacos , Nanopartículas/toxicidade , Poluentes Químicos da Água/toxicidade , Cério/química , Água Doce/química , Nanopartículas/química , Poluentes Químicos da Água/químicaRESUMO
In this work, we present new nanocomposite materials derived from segmented copolyesters, comprising ethylene terephthalate (PET) segments and dimerized linoleic acid (DLA), and nanometric cerium oxide particles (CeO2). Nanoparticles were incorporated in situ during polycondensation in various concentrations, from 0.1 up to 0.6 wt.%. It was found that preparation of nanocomposites in situ, during polycondensation, had no significant influence on changes in segmental composition as determined from (1)H and (13)C, as well as 2D NMR. Thermal analysis and calculated degree of crystallinity showed that increasing concentration of ceria nanoparticles lead to an increase in mass content of PET crystallites in hard segments. The XRD investigations also showed an increased intensity of characteristic signals with increasing ceria concentration. Simultaneously, the incorporation of CeO2 led to an increase in tensile strength and elongation at break, indicating a reinforcing and plasticizing effect of ceria nanoparticles. However, the modulus at 10% strain decreased with increasing amount of nanoparticles. The in vitro culture of human cardiac progenitor cells (hCPCs) on the new materials indicated a homogenous cell displacement across the samples after 5 days with no signs of cytotoxicity, indicating good biocompatibility in vitro of CeO2-based nanocomposites and a potential for biomedical applications.
Assuntos
Materiais Biocompatíveis/química , Cério/química , Nanopartículas Metálicas/química , Nanocompostos/química , Poliésteres/química , Idoso , Idoso de 80 Anos ou mais , Materiais Biocompatíveis/toxicidade , Forma Celular/efeitos dos fármacos , Células Cultivadas , Cério/toxicidade , Feminino , Humanos , Masculino , Nanopartículas Metálicas/toxicidade , Pessoa de Meia-Idade , Nanocompostos/toxicidade , Poliésteres/toxicidade , ViscosidadeRESUMO
Silver nanoparticles (Ag NP) have been shown to generate reactive oxygen species; however, the association between physicochemical characteristics of nanoparticles and cellular stress responses elicited by exposure has not been elucidated. Here, we examined three key stress-responsive pathways activated by Nrf-2/ARE, NFκB, and AP1 during exposure to Ag NP of two distinct sizes (10 and 75 nm) and coatings (citrate and polyvinylpyrrolidone), as well as silver nitrate (AgNO3), and CeO2 nanoparticles. The in vitro assays assessed the cellular response in a battery of stable luciferase-reporter HepG2 cell lines. We further assessed the impact of Ag NP and AgNO3 exposure on cellular redox status by measuring glutathione depletion. Lastly, we determined intracellular Ag concentration by inductively coupled plasma mass spectroscopy (ICP-MS) and re-analyzed reporter-gene data using these values to estimate the relative potencies of the Ag NPs and AgNO3. Our results show activation of all three stress response pathways, with Nrf-2/ARE displaying the strongest response elicited by each Ag NP and AgNO3 evaluated here. The smaller (10-nm) Ag NPs were more potent than the larger (75-nm) Ag NPs in each stress-response pathway, and citrate-coated Ag NPs had higher intracellular silver concentrations compared with both PVP-coated Ag NP and AgNO3. The cellular stress response profiles after Ag NP exposure were similar to that of AgNO3, suggesting that the oxidative stress and inflammatory effects of Ag NP are likely due to the cytotoxicity of silver ions.
Assuntos
Nanopartículas Metálicas/toxicidade , Prata/toxicidade , Transporte Biológico , Sobrevivência Celular , Cério/toxicidade , Ácido Cítrico/química , Genes Reporter , Glutationa/metabolismo , Células Hep G2 , Humanos , Luciferases/genética , Nanopartículas Metálicas/química , Fator 2 Relacionado a NF-E2/genética , NF-kappa B/genética , Estresse Oxidativo/efeitos dos fármacos , Tamanho da Partícula , Povidona/química , Proteína de Replicação C/genética , Prata/química , Nitrato de Prata/toxicidadeRESUMO
Effects of CeO2-NPs on anaerobic fermentation were investigated from the processes of acidification and methanation with anaerobic granule sludge and anaerobic flocculent sludge as the targets. Results showed that acidification process was more sensitive to CeO2-NPs than methanation process. Both types of sludge produced less short-chain fatty acid compared to the control, with a reduction of 15-19% for the flocculent sludge at the dosage of 5, 50 and 150 mg CeO2-NPs/g-VSS, and a reduction of 35% for the granular sludge at 150 mg CeO2-NPs/g-VSS. CeO2-NPs caused no inhibition to methanation process. Most of CeO2-NPs distributed on the surface of sludge as revealed by fluorescence labeled CeO2-NPs. The toxicity of CeO2-NPs to anaerobic sludge did not result from reactive oxygen species. Physical penetration and membrane reduction may be important toxic mechanisms.
Assuntos
Cério/toxicidade , Nanopartículas/toxicidade , Esgotos/microbiologia , Anaerobiose/efeitos dos fármacos , Biopolímeros/análise , Espaço Extracelular/química , Ácidos Graxos/metabolismo , Floculação/efeitos dos fármacos , L-Lactato Desidrogenase/metabolismo , Metano/metabolismo , Viabilidade Microbiana/efeitos dos fármacos , Espécies Reativas de Oxigênio/metabolismoRESUMO
Water-dispersible Re(3+) doped CeF(3) colloidal nanocrystals with well controllable morphology and high crystallinity have been successfully synthesized through a solvothermal process. The TEM images illustrate that the Re(3+) doped CeF(3) nanocrystals are rectangular (or cubic) with a mean diameter of ~10 nm. The excellent dispersibility in some of the polar solvents including water is achieved by using polyethyleneimine as the capping agent. The amine groups of the polymer chains on one hand bind to the nanocrystal surface; on the other hand the free ones could link to functional materials including bio-molecules. The CeF(3) nanocrystals doped with Tb(3+) and Dy(3+) ions show the characteristic emission of Tb(3+ 5)D(4)-(7)F(J) (J = 6-3, with (5)D(4)-(7)F(5) green emission at 542 nm as the strongest one) and Dy(3+ 4)F(9/2)-(6)H(15/2) (blue-green color at 478 nm) and (4)F(9/2)-(6)H(13/2) (yellow color at 571 nm) transitions, respectively. The energy transfer from Ce(3+) to Tb(3+) and Dy(3+) was also investigated in detail. In vitro studies of Re(3+) doped CeF(3) colloidal nanocrystals on HepG2 cells confirm their excellent biological compatibility. The obtained solid CeF(3) : Tb(3+)/PDMS nanocomposites are very stable and flexible and exhibit strong green photoluminescence upon UV excitation.